Article
Fisheries
Suparat Taengchaiyaphum, Jiraporn Srisala, Piyachat Sanguanrut, Chalermporn Ongvarrasopone, Timothy W. Flegel, Kallaya Sritunyalucksana
Summary: LSNV, discovered in 2006, is proposed as a cause of retarded growth in giant tiger shrimp. Recent research suggests that LSNV and WZSV 9 may be different isolates of the same virus species.
Article
Genetics & Heredity
Md. Mehedi Hasan, Herman W. Raadsma, Peter C. Thomson, Nicholas M. Wade, Dean R. Jerry, Mehar S. Khatkar
Summary: This study investigated the phenotypic and genetic variances and relationships for body weight and body color traits of black tiger shrimp. It found that the color of cooked shrimp can be improved indirectly by genetic selection based on the color of uncooked/live shrimp. Additionally, the intensity of coloration is positively correlated with body weight traits.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Medicinal
Yujian Wang, Jian Zhang, Yuanyuan Sun, Li Sun
Summary: Crus1, a type of antimicrobial peptide from deep-sea shrimp, effectively kills Gram-positive bacteria by inducing membrane leakage and structure damage in a pH, temperature, and disulfide linkage-dependent manner. Serine substitution of cysteine residues in the WAP domain does not affect bacterial binding capacity but completely abolishes the bactericidal activity of rCrus1.
Article
Fisheries
Chao Zhao, Chao Peng, Pengfei Wang, Bo Zhang, Lulu Yan, Chun-Lin Wang, Lihua Qiu
Summary: In this study, a novel TRIM37 homolog called PmTRIM37 was identified, which plays important roles in regulating innate immune processes, particularly antiviral and proinflammatory cytokine responses. PmTRIM37 is highly expressed in immune-related tissues and its expression is up-regulated after white spot syndrome virus (WSSV) infection. Knockdown of PmTRIM37 promotes WSSV replication and VP28 expression, suggesting its negative role in WSSV infection. Furthermore, PmTRIM37 positively regulates the NF-κB pathway and Antimicrobial peptides (AMP) expression during WSSV infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Natthida Rakbanjong, Tomoyuki Okutsu, Wilaiwan Chotigeat, Anida Songnui, Monwadee Wonglapsuwan
Summary: This study compared the efficiency of different cryoprotectants and thawing temperatures on germ cell cryopreservation in two marine shrimp species, finding that 10% DMSO and 10% GLY with vitrification are suitable for Fenneropenaeus merguiensis and Penaeus monodon, respectively. Thawing temperature was also found to have an impact on cell viability.
MARINE BIOTECHNOLOGY
(2021)
Article
Immunology
Yundong Li, Falin Zhou, Qibin Yang, Song Jiang, Jianhua Huang, Lishi Yang, Zhenhua Ma, Shigui Jiang
Summary: This study used single-cell transcriptome sequencing to construct a single-cell transcriptional map of hepatopancreatic cells and haemocytes in shrimp, and analyzed the molecular mechanisms of the immune response to ammonia nitrogen stress. The results identified the developmental starting points of haemocytes and hepatopancreatic cells, and revealed the activation of antioxidant and immune defense systems under ammonia nitrogen stress. Important response genes played a role in shrimp cell differentiation and functional plasticity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rui Fan, Yundong Li, Qibin Yang, Song Jiang, Jianhua Huang, Lishi Yang, Xu Chen, Falin Zhou, Shigui Jiang
Summary: In this study, a novel glutaredoxin (Grx), named PmGrx2, was identified in Penaeus monodon. The study showed that PmGrx2 plays an important role in shrimps' resistance to marine environmental stresses. In addition, inhibition of PmGrx2 resulted in shrimp being exposed to a greater risk of oxidative damage.
Article
Fisheries
Kai Xu, Weili Wang, Dong Liu, Chao Wang, Jun Zhu, Binlun Yan, Huan Gao, Guangwei Hu
Summary: This study identified a novel crustin-like peptide, EcCrustin2, which was found to be mainly expressed in immune tissues and demonstrated antibacterial activity. These findings suggest that EcCrustin2 plays a crucial role in the immune response against bacteria and EHP infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Pacharaporn Angthong, Tanaporn Uengwetwanit, Sopacha Arayamethakorn, Wanilada Rungrassamee
Summary: The study identified the establishment of the immune system in shrimp during early larval stages through gene analysis. The Toll pathway was found as a baseline immune system established in the nauplius stage, with immune defense mechanisms like pathogen pattern-recognition proteins, antimicrobial peptides, and heat shock proteins established in later larval stages.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Linxin Dai, Peixun Chen, Danqing Hou, Yue Wang, Yinhuan Zhou, Chengbo Sun
Summary: This study isolated and identified a strain of Vibrio fortis from a biofloc pool, and investigated its pathogenicity and antibiotic sensitivity in Penaeus monodon. The study also explored the immune response and molecular changes in P. monodon infected with V. fortis. The findings provide insights into the study and control of shrimp vibriosis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Md Iftakharul Alam, Sanjida Yeasmin, Mst Muslima Khatun, Md Moshiur Rahman, Moin Uddin Ahmed, Adolphe O. Debrot, Md Nazmul Ahsan, M. C. J. Verdegem
Summary: Color, texture, and flavor are important factors in determining the quality and value of seafood products. This study found that leaf litter from mangrove species can enhance the production and color quality of black tiger shrimp, providing a viable technique for commercial shrimp aquaculture.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Wen-Ya Wei, Jian-Hua Huang, Qi-Bin Yang, Fa-Lin Zhou, Song Jiang, Yun-Dong Li, Shi-Gui Jiang, Li-Shi Yang
Summary: As a highly conserved transcription factor family, DMRTs play important roles in sex determination and sexual differentiation in various animals. In this study, a DM-domain gene named PmDMRT11E was identified in Penaeus monodon, and it was found to be highly expressed in the testis and play a crucial regulatory role during embryonic development. Additionally, PmDMRT11E was found to be closely related to gonadal development and spermatogenesis.
AQUACULTURE REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
T. H. Noble, F. Samsing, N. Wade, S. Osborne, R. Addepalli, J. W. Wynne
Summary: Immune stimulation through feed additives, such as the LPS component in NovacqTM, can effectively combat diseases in shrimp farming and reduce antibiotic usage. In this study, lipopolysaccharide-like molecules from NovacqTM were found to stimulate a rapid and significant induction of the phenoloxidase response in shrimp hemocytes. Transcriptome analysis revealed an up-regulation of immune-related genes and the activation of transcription factors in the hemocytes, indicating the strong immunostimulatory ability of NovacqTM.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2023)
Article
Fisheries
Hui Guo, Jian-An Xian, Pei-Hua Zheng, Yao-Peng Lu, Lei Wang, Xiu-Xia Zhang, Jun-Tao Li, Ze-Long Zhang, An-Li Wang
Summary: Juvenile tiger shrimp fed diets with 40 mg/kg Cu showed the highest weight gain, while excessive Cu intake at 110 mg/kg led to oxidative stress and haemocyte apoptosis. Dietary intake of Cu at 25-55 mg/kg contributed to higher antioxidant enzyme activities and total antioxidant capacity, but 110 mg/kg Cu intake increased oxidative stress.
AQUACULTURE REPORTS
(2022)
Article
Food Science & Technology
Narin Charoenphun, Bharathipriya Rajasekaran, Suguna Palanisamy, Karthikeyan Venkatachalam
Summary: The objective of this study was to evaluate the impact of varying concentrations of longkong pericarp extract on the physicochemical properties of alginate-based edible nanoparticle coatings on shrimp. The highest LPE concentration (1.5%) led to improved quality and extended shelf-life of shrimp during refrigerated storage.
Article
Fisheries
Phattarunda Jaree, Pakpoom Boonchuen, Jumroensri Thawonsuwan, Hidehiro Kondo, Ikuo Hirono, Kunlaya Somboonwiwat
Summary: The study investigated the relationship between metabolic gene expression and antiviral responses in shrimp, identifying over 130 DEGs related to WSSV infection that were associated with innate immunity and metabolic pathways. The results suggested a link between the up-regulation of the rate-limiting enzyme FmGFAT in the hexosamine biosynthesis pathway and WSSV responses, impacting viral replication in shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Virology
Satoshi Kawato, Shoya Fujishima, Reiko Nozaki, Hidehiro Kondo, Tadashi Isshiki, Ikuo Hirono
Summary: This study reports the causative agent of milky hemolymph disease in snow crab, known as Chionoecetes opilio bacilliform virus (CoBV), and reveals its complete genome sequence, confirming its classification as a member of the Nimaviridae family.
ARCHIVES OF VIROLOGY
(2023)
Article
Fisheries
Liu Wang, Keisuke Yoshii, Naoya Murase, Hidetoshi Yamada, Yutaka Fukuda, Ikuo Hirono, Hidehiro Kondo
Summary: This study aimed to develop a better immunogenic and protective fish vaccine and compare the effectiveness of poly I:C and formalin-killed cells (FKCs) as adjuvants against pathogen infection. The results showed that co-inoculation with poly I:C and FKC significantly increased the expression levels of specific antibodies and provided better protection compared to FKC vaccine alone.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Mahmoud Mostafa Mahmoud, Mohamed Abdelsalam, Satoshi Kawato, Shogo Harakawa, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study compared the whole genome sequences of three different serotypes of Lactococcus garvieae isolated from diseased cultured striped jack in Ehime prefecture, Japan. The serotypes showed different virulence in a challenge test. Two serotypes were identified as L. garvieae and one as L. formosensis. The genome sizes ranged from 1.9 to 2.0 Mb, GC content ranged from 38.2% to 38.9%, and the number of predicted protein-coding sequences ranged from 1922 to 1959. Only one serotype harbored two plasmids.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Jayasundara Mudiyanselage Sajani Nisansala Rathnapala, Wafaa Ragab, Satoshi Kawato, Miho Furukawa, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: By sequencing the genomes of three strains of Vibrio nigripulchritudo, it was found that they possess various virulence factors and mobile genetic elements, as well as multidrug resistance. Comparative genomic analysis showed that all three strains belong to the same clade.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, Drop-seq technology was used to investigate the impact of virus infection on hemocyte populations in shrimp. The findings revealed that virus infection reduced specific cell populations in circulating hemolymph and inhibited the expression of antimicrobial peptides. Unknown genes with antimicrobial activity were also identified. Additionally, the study discussed the effects of methanol fixation on Drop-seq data.
MARINE BIOTECHNOLOGY
(2023)
Article
Fisheries
Chalermkwan Eurlaphan, Reiko Nozaki, Motohiko Sano, Keiichiro Koiwai, Ikuo Hirono, Hidehiro Kondo
Summary: This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus). The results showed that viral gene transcripts were detected at 3 and 5 days post-injection, and certain host genes were differentially expressed during the infection process. The downregulation of certain inflammation-related genes may contribute to the increased susceptibility of this fish to the virus.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Hidehiro Kondo, Haruka Kamei, Fuyuka Murotani, Reiko Nozaki, Ryosuke Yazawa, Reoto Tani, Keiichiro Koiwai, Ikuo Hirono
Summary: A novel immunoglobulin heavy chain-like gene (tsIgH) was identified in the liver of the banded houndshark Triakis scyllium through preliminary transcriptomic analysis. The tsIgH gene had low amino acid sequence similarities (< 30%) to shark Ig genes. It encoded one variable domain (VH) and three conserved domains (CH1-CH3) with a predicted signal peptide. The gene structure and expression pattern suggest that this gene may provide insights into the evolution of immunoglobulin genes in cartilaginous fishes.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
Article
Fisheries
Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
Summary: The live attenuated vaccine strain P7-P8 demonstrates high protective efficacy against herpesviral haematopoietic necrosis caused by cyprinid herpesvirus 2 (CyHV-2) in goldfish at temperatures ranging from 15 to 30 degrees C. The vaccine virus load over 104 DNA copies/mg may elicit sufficient acquired immunity, and cell-mediated immunity may play a fundamental role in protection.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, the genomes of 27 endogenous nimaviruses were recovered from crustacean genome data. Phylogenetic analysis revealed different lineages and specific insertion preferences for each nimavirus species. These findings suggest that endogenous nimaviruses are giant transposable elements occupying different sequence niches.
MICROBIOLOGY SPECTRUM
(2023)