Article
Anatomy & Morphology
Sarah K. Mierke, Kelsey L. Rapier, Anna M. Method, Brooke A. King, Paul S. Kingma
Summary: Surfactant protein D (SP-D) is a host defense protein that clears infectious pathogens from the lung. This study found that SP-D is also present in plasma and other non-pulmonary tissues. The researchers hypothesized that systemic SP-D may clear infectious pathogens and regulate host defense cells in extrapulmonary systems. Their results suggest that circulating SP-D decreases systemic inflammation and raises the possibility that its increased levels during infection serve to scavenge infectious pathogens and limit inflammation-induced tissue injury.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2023)
Article
Engineering, Biomedical
Marinus A. Wesdorp, Andrea Schwab, Ezgi Irem Bektas, Roberto Narcisi, David Eglin, Martin J. Stoddart, Gerjo J. V. M. Van Osch, Matteo D'Este
Summary: This study presents an in vitro culture model that describes the important functions of neutrophils in tissue repair. The response of neutrophils to different biomaterials was investigated, and differences in neutrophil survival, oxidative burst, myeloperoxidase and neutrophil elastase expression, and cytokine secretion were observed.
BIOACTIVE MATERIALS
(2023)
Article
Environmental Sciences
Pilar Requena, Celia Perez-Diaz, Vicente Mustieles, Francisco M. Peinado, Josefa Leon, Francisco M. Perez-Carrascosa, Hanne Frederiksen, Inmaculada Salcedo-Bellido, Rocio Barrios-Rodriguez, Juan Pedro Arrebola
Summary: This study investigates the associations between human phthalate exposure and the onset of chronic diseases with an immunological component. The results suggest that certain phthalates are correlated with cytokines and acute phase proteins, indicating potential subclinical immuno-inflammatory actions of these pollutants.
ENVIRONMENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ito Hirasawa, Haruka Odagiri, Giri Park, Rutvi Sanghavi, Takaya Oshita, Akiko Togi, Katsunori Yoshikawa, Koji Mizutani, Yasuo Takeuchi, Hiroaki Kobayashi, Sayaka Katagiri, Takanori Iwata, Akira Aoki
Summary: Cold atmospheric plasma (CAP) shows promise as a treatment for diseases involving chronic inflammation and bacterial infections. This study aimed to elucidate the anti-inflammatory mechanisms of CAP and found that it suppresses the inflammatory response by promoting nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2).
Review
Dentistry, Oral Surgery & Medicine
Lena Larsson, Carlos Garaicoa-Pazmino, Farah Asa'ad, Rogerio Moraes Castilho
Summary: This review presents the current understanding of endotoxin tolerance (ET) in chronic inflammatory diseases and explores its potential connection with periodontitis. Mechanisms and pathways such as Toll-like receptors, nuclear factor κB, immune cell apoptosis, epigenetics, and microRNAs regulate ET and contribute to chronic inflammatory diseases including periodontitis. While the direct relationship between ET and periodontal destruction is not fully understood, evidence suggests potential tolerization of periodontal ligament cells and gingival tissues with reduced levels of Toll-like receptors. Further research is needed to investigate the crucial role of ET in the pathogenesis of periodontal diseases.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Review
Clinical Neurology
Guy C. Brown, Marta Camacho, Caroline H. Williams-Gray
Summary: The endotoxin hypothesis suggests that lipopolysaccharide (LPS) endotoxins contribute to the pathogenesis of Parkinson's disease through gut dysfunction, elevated LPS levels, inflammation, and neurodegeneration. Evidence supporting this hypothesis includes early gut dysfunction, increased LPS levels in some PD patients, and the ability of LPS to induce alpha-synuclein expression and neurotoxicity.
MOVEMENT DISORDERS
(2023)
Article
Medicine, General & Internal
Farah Omran, Alice M. Murphy, Awais Z. Younis, Ioannis Kyrou, Jana Vrbikova, Vojtech Hainer, Petra Sramkova, Martin Fried, Graham Ball, Gyanendra Tripathi, Sudhesh Kumar, Philip G. McTernan, Mark Christian
Summary: This study investigated the impact of endotoxin on mitochondrial function and browning capacity in human adipocytes. The results showed that endotoxin directly contributes to impaired mitochondrial function and reduced browning capacity of adipocytes, exacerbating metabolic consequences. Bariatric surgery, which reduces endotoxin levels, is associated with improved adipocyte functionality.
Article
Cardiac & Cardiovascular Systems
Michael Resl, Matthias Wolfgang Heinzl, Carmen Klammer, Margot Egger, Roland Feldbauer, Johannes Pohlhammer, Benjamin Dieplinger, Martin Clodi
Summary: Acute inflammation has a significant impact on the cardiovascular system, leading to changes in a large number of cardiovascular biomarkers. Experimental low-dose inflammation treatment resulted in enormous pressure on the cardiovascular system in response, affecting the majority of the biomarkers tested.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Xin Shi, Shaopin Zhu, Huiyi Jin, Junwei Fang, Xindan Xing, Yihan Wang, Hanying Wang, Chingyi Wang, Tian Niu, Kun Liu
Summary: The study revealed that KS23 had therapeutic effects on endotoxin-induced uveitis in rats, reduced infiltration of inflammatory cells, and levels of inflammatory cytokines. It also showed inhibitory effects on LPS-stimulated RAW 264.7 cells, suggesting KS23 as a promising therapeutic candidate for ocular inflammation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhengjiang Qian, Chunhua Liu, Hongchao Li, Haiyang Yang, Jianhao Wu, Jing Liu, Yanjiao Li, Xuhui Chen, Jianyang Xu, Xiang Li
Summary: This study demonstrates the protective role of osteocalcin (OCN) in macrophages against lipopolysaccharide (LPS)-caused acute inflammation through the activation of G protein-coupled receptor 37 (GPR37). OCN can regulate inflammatory responses and macrophage-associated functions by interacting with GPR37. The findings suggest that targeting the OCN/GPR37 regulatory axis may be a potential therapeutic strategy for inflammatory diseases.
Article
Immunology
Sven Benson, Franziska Labrenz, Simone Kotulla, Lisa Brotte, Philipp Roedder, Bastian Tebbe, Nina Theysohn, Harald Engler, Sigrid Elsenbruch
Summary: This study tested the interaction effects of acute systemic inflammation and sad mood on the expectation and experience of visceral pain. The results indicate that there is an interplay between inflammation and negative mood in the perception and interpretation of visceral pain.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Pharmacology & Pharmacy
Wael A. A. Alanazi, Turki Alharbi, Doaa M. M. El-Nagar, Abdullah M. M. Albogami, Mohammed Alswayyed
Summary: In this study, it was found that the SGLT2 inhibitor dapagliflozin attenuated lipopolysaccharide-induced hypotension by inhibiting vasodilation and reducing cytokine levels. This protective effect was independent of glycemic control and applicable to patients with different glucose levels.
Article
Medicine, Research & Experimental
Hassan Ahmed, Urooj Amin, Xiaolun Sun, Demetrius R. Pitts, Yunbo Li, Hong Zhu, Zhenquan Jia
Summary: Lipopolysaccharide (LPS) is an endotoxin that can cause severe inflammation and septic shock. This study shows that the triterpenoid compound CDDO-IM can reduce LPS-induced inflammation in macrophage cells and protect them from cytotoxicity by upregulating antioxidant molecules.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Huanling Yao, Guojie Cao, Zheng Liu, Yue Zhao, Zhanchao Yan, Senzhen Wang, Yuehua Wang, Zhengwei Guo, Yanming Wang
Summary: Inhibition of NET formation can alleviate inflammatory damage and prolong the survival time of mice under endotoxic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Tucker J. Piergallini, Julia M. Scordo, Paula A. Pino, Larry S. Schlesinger, Jordi B. Torrelles, Joanne Turner
Summary: The study demonstrates that an early and transient inflammatory environment induced by lipopolysaccharide treatment enhances protection against Mycobacterium tuberculosis infection in mice for up to 6 months postinfection. Neutrophils from lipopolysaccharide-treated mice exhibited increased association with M. tuberculosis and had a greater innate capacity for killing the bacteria. The results suggest that acute inflammation at the time of M. tuberculosis infection can positively alter disease outcome by potentially improving immune responses.
MICROBIOLOGY SPECTRUM
(2021)
Article
Pediatrics
Helene Widowski, Daan R. M. G. Ophelders, Anais J. C. N. van Leeuwen, Peter G. J. Nikkels, Carmen A. H. Severens-Rijvers, Vanessa L. S. LaPointe, Jack P. M. Cleutjens, Matthias C. Hutten, Matthew W. Kemp, Matthew S. Payne, Masatoshi Saito, Haruo Usuda, John P. Newnham, Alan H. Jobe, Boris W. Kramer, Tammo Delhaas, Tim G. A. M. Wolfs, Niki L. Reynaert
Summary: In an ovine chorioamnionitis model, exposure to LPS or Ureaplasma parvum in utero resulted in altered lung function and changes in stem/progenitor cell pools, potentially disrupting normal lung development and leading to adverse postnatal outcomes.
PEDIATRIC RESEARCH
(2021)
Article
Cell & Tissue Engineering
Ruth Gussenhoven, Daan R. M. G. Ophelders, Jeroen Dudink, Kay Pieterman, Martin Lammens, Robert W. Mays, Luc J. Zimmermann, Boris W. Kramer, Tim G. A. M. Wolfs, Reint K. Jellema
Summary: This study demonstrated that early systemic MAPC therapy improved structural injury of the preterm cerebellum following global HI in a preclinical large animal model. Microstructural improvement was detectable with DTI. These findings support the potential of MAPC therapy for the treatment of HIE and the added clinical value of DTI for the detection of cerebellar injury and the evaluation of cell-based therapy.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Charlotte van Gorp, Ilse H. de Lange, Kimberly R. I. Massy, Lilian Kessels, Alan H. Jobe, Jack P. M. Cleutjens, Matthew W. Kemp, Masatoshi Saito, Haruo Usada, John Newnham, Matthias Hutten, Boris W. Kramer, Luc J. Zimmermann, Tim G. A. M. Wolfs
Summary: Research in an ovine chorioamnionitis model revealed a biphasic reduction in goblet cell numbers, with the second decrease associated with intestinal inflammation, apoptosis, and ER stress. The findings suggest that ER stress leads to apoptosis of maturing goblet cells, ultimately reducing their numbers. This insight could be important for understanding the pathogenesis of NEC and developing potential therapeutic strategies targeting ER stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Helene Widowski, Niki L. Reynaert, Daan R. M. G. Ophelders, Matthias C. Hutten, Peter G. J. Nikkels, Carmen A. H. Severens-Rijvers, Jack P. M. Cleutjens, Matthew W. Kemp, John P. Newnham, Masatoshi Saito, Haruo Usuda, Matthew S. Payne, Alan H. Jobe, Boris W. Kramer, Tammo Delhaas, Tim G. A. M. Wolfs
Summary: The study utilized a sheep model to investigate the impact of antenatal infection and inflammation on lung development in preterm infants. Re-exposure to inflammatory stimuli may result in impaired alveolarization and abnormal pulmonary vascular development. Antenatal infection may play a significant role in lung development.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Nathalie Vaes, Simone L. Schonkeren, Glenn Rademakers, Amy M. Holland, Alexander Koch, Marion J. Gijbels, Tom G. Keulers, Meike de Wit, Laura Moonen, Jaleesa R. M. Van der Meer, Edith van den Boezem, Tim G. A. M. Wolfs, David W. Threadgill, Jeroen Demmers, Remond J. A. Fijneman, Connie R. Jimenez, Pieter Vanden Berghe, Kim M. Smits, Kasper M. A. Rouschop, Werend Boesmans, Robert M. W. Hofstra, Veerle Melotte
Summary: The absence of Ndrg4 does not cause functional or morphological abnormalities in the gastrointestinal tract, but it is associated with larger intestinal adenoma development. ENS-derived Nidogen-1 and Fibulin-2 enhance colorectal carcinogenesis by boosting organoid growth and promoting migration capacities of CRC cells.
Review
Nutrition & Dietetics
Ilse H. de Lange, Charlotte van Gorp, Laurens D. Eeftinck Schattenkerk, Wim G. van Gemert, Joep P. M. Derikx, Tim G. A. M. Wolfs
Summary: This article summarizes the pathogenesis of necrotizing enterocolitis (NEC), highlighting the immaturity of the intestinal immune system in preterm infants and abnormal microbial colonization as key factors. Enteral feeding is considered an important factor influencing NEC pathogenesis, with bioactive components in breast milk being effective in preventing NEC.
Article
Multidisciplinary Sciences
Cathelijne Heymans, Marcel den Dulk, Kaatje Lenaerts, Lara R. Heij, Ilse H. de Lange, Mhamed Hadfoune, Chantal van Heugten, Boris W. Kramer, Alan H. Jobe, Masatoshi Saito, Matthew W. Kemp, Tim G. A. M. Wolfs, Wim G. van Gemert
Summary: Research indicates that intra-amniotic LPS exposure causes time-dependent changes in fetal liver and enterohepatic circulation in lambs, including hepatic inflammation and increased bile acid levels, but EHC alterations normalize over time. These changes may have postnatal consequences, highlighting the potential importance of the duration of intra-amniotic LPS exposure.
SCIENTIFIC REPORTS
(2021)
Article
Pediatrics
Kathryn A. Martinello, Christopher Meehan, Adnan Avdic-Belltheus, Ingran Lingam, Tatenda Mutshiya, Qin Yang, Mustafa Ali Akin, David Price, Magdalena Sokolska, Alan Bainbridge, Mariya Hristova, Ilias Tachtsidis, Cally J. Tann, Donald Peebles, Henrik Hagberg, Tim G. A. M. Wolfs, Nigel Klein, Boris W. Kramer, Bobbi Fleiss, Pierre Gressens, Xavier Golay, Nicola J. Robertson
Summary: Therapeutic hypothermia did not show protection in a piglet model of inflammation-sensitized hypoxia-ischemia, as evidenced by aEEG, MRS, and immunohistochemistry. The immunosuppressive effects of hypothermia and the counteracting neuroinflammation by LPS may have led to the lack of efficacy of hypothermia in this context. Other immunomodulatory strategies may be more effective in treating inflammation-sensitized hypoxia-ischemia injuries.
PEDIATRIC RESEARCH
(2022)
Article
Pediatrics
Daan R. M. G. Ophelders, Agnes W. Boots, Matthias C. Hutten, Salwan Al-Nasiry, Reint K. Jellema, Owen B. Spiller, Frederik-Jan van Schooten, Agnieszka Smolinska, Tim G. A. M. Wolfs
Summary: Chorioamnionitis is a major risk factor for preterm birth and postnatal morbidity. This study found that a specific volatile organic compound (VOC) profile in the exhaled breath of pregnant sheep may accurately diagnose intra-amniotic infection. The distinct VOC signature induced by Ureaplasma parvum infection can differentiate between infected and non-infected animals, providing insight into the temporal dynamics of intra-amniotic infection.
FRONTIERS IN PEDIATRICS
(2021)
Article
Pediatrics
Lieke A. Hoogenboom, A. Titia Lely, Matthew W. Kemp, Masatoshi Saito, Alan H. Jobe, Tim G. A. M. Wolfs, Michiel F. Schreuder
Summary: This study investigated the impact of premature birth and chorioamnionitis on kidney development using a sheep model. The findings suggest that chorioamnionitis induced by LPS leads to inflammation, podocyte injury, and potential fibrotic changes in the glomerular mesangium.
FRONTIERS IN PEDIATRICS
(2022)
Article
Nanoscience & Nanotechnology
Tianyu Yao, Teun van Nunen, Rebeca Rivero, Chadwick Powell, Ryan Carrazzone, Lilian Kessels, Paul Andrew Wieringa, Shahzad Hafeez, Tim G. A. M. Wolfs, Lorenzo Moroni, John B. Matson, Matthew B. Baker
Summary: This study developed an angiogenic scaffold by covalently attaching a hydrogen sulfide donor to a polycaprolactone scaffold, enabling sustained release of hydrogen sulfide. In vitro experiments showed that the functionalized scaffold promoted endothelial cell proliferation and formation of tight cell-cell junctions. In vivo experiments demonstrated increased neovascularization. These findings suggest that this functionalized scaffold provides localized control over vascularization and has potential applications in tissue engineering.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nutrition & Dietetics
Ilse H. de Lange, Charlotte van Gorp, Kimberly R. I. Massy, Lilian Kessels, Nico Kloosterboer, Ann Bjornshave, Marie Stampe Ostenfeld, Jan G. M. C. Damoiseaux, Joep P. M. Derikx, Wim G. van Gemert, Tim G. A. M. Wolfs
Summary: Many whey proteins, peptides, and protein-derived amino acids can improve gut health through their anti-oxidant, anti-microbial, barrier-protective, and immune-modulating effects. This study investigated the effects of whey protein fractions with different degrees of enzymatic hydrolysis on the intestinal epithelium, as well as their anti-microbial and immune effects. The results highlight the importance of hydrolysis degree in influencing the biological effects of whey protein fractions, and provide new insights for improving immune tolerance and promoting intestinal health.
Article
Engineering, Biomedical
Tianyu Yao, Honglin Chen, Rong Wang, Rebeca Rivero, Fengyu Wang, Lilian Kessels, Stijn M. Agten, Tilman M. Hackeng, Tim G. A. M. Wolfs, Daidi Fan, Matthew B. Baker, Lorenzo Moroni
Summary: This study focuses on the functionalization of scaffolds using a polymer mixing approach to immobilize VEGF-mimetic peptides. The functionalized scaffolds were validated and tested, showing potential for promoting angiogenesis and vascular tissue regeneration.
BIOACTIVE MATERIALS
(2023)
Article
Medicine, Research & Experimental
Charlotte van Gorp, Ilse H. de Lange, Matthias C. Hutten, Carmen Lopez-Iglesias, Kimberly Massy, Lilian Kessels, Boris Kramer, Willine van de Wetering, Brad Spiller, George M. Birchenough, Wim G. van Gemert, Luc J. Zimmermann, Tim Wolfs
Summary: Disruption of the intestinal mucus barrier and intestinal epithelial endoplasmic reticulum stress contribute to necrotizing enterocolitis. In this study, the authors investigated the effects of chorioamnionitis on goblet cells, and found that exposure to Ureaplasma parvum serovar-3 led to thickened ileal mucus layer and mucus hypersecretion from goblet cells in fetal lambs. Furthermore, similar cellular morphological alterations were observed in human NEC samples, highlighting the clinical relevance of these in utero findings.
Article
Immunology
Luise Klein, Daan R. M. G. Ophelders, Daniel van den Hove, Maurits Damoiseaux, Bart P. F. Rutten, Chris P. M. Reutelingsperger, Leon J. Schurgers, Tim G. A. M. Wolfs
Summary: Systemic and cerebral inflammation following antenatal infection and dysregulation of the blood brain barrier can lead to abnormal neonatal brain development. Administration of multipotent adult progenitor cells (MAPCs) can modulate the immune response and protect the integrity of the blood brain barrier. In a preclinical ovine model, prenatal administration of MAPCs increased blood brain barrier integrity and protected the neonatal brain against inflammation caused by infection.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)
