Article
Agriculture, Dairy & Animal Science
Manman Shen, Tao Li, Yuan Feng, Ping Wu, Boris Ramos Serrano, Adileidys Ruiz Barcenas, Liang Qu, Weiguo Zhao
Summary: This study found that the flavonoid quercetin has a regulatory effect on granulosa cells (GCs) in the prehierarchical follicles of chickens. Specifically, at appropriate concentrations, quercetin can promote cell proliferation and progesterone secretion, which is beneficial for follicle development and selection.
Article
Biochemistry & Molecular Biology
Huihua Wu, Rui Zhu, Bo Zheng, Guizhi Liao, Fuxin Wang, Jie Ding, Hong Li, Mingqing Li
Summary: This study provides a comprehensive understanding of the follicular microenvironment at the single-cell level. The functions and cellular components of the follicular microenvironment, including granulosa cells (GCs) and immune cells, are explored. GCs are found to have different functional clusters involved in inflammatory responses and adhesive function, while follicular macrophages play roles in immune responses and extracellular matrix remodeling, assisting GCs in promoting oocyte maturation. The specific subclusters of GCs with high levels of adhesive-related molecules contribute to the recruitment and residence of macrophages, leading to heterogeneity in the proportion of immune cells in preovulatory follicles from different patients.
Article
Biochemistry & Molecular Biology
Rikki N. Nelson, V. Praveen Chakravarthi, Anamika Ratri, Xiaoman Hong, Jan A. Gossen, Lane K. Christenson
Summary: Loss of ADAR1 in GCs leads to increased expression of inflammatory and interferon response genes, resulting in ovulation failure, alterations in oocyte developmental progression, and infertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
E. R. Frost, E. A. Ford, G. Taylor, S. Boeing, E. L. Beckett, S. D. Roman, R. Lovell-Badge, E. A. McLaughlin, J. M. Sutherland
Summary: The need for investigations has driven the development of ovarian cell dissociation techniques, including two methods suitable for different developmental stages of ovarian samples. These methods provide important tools for the study of folliculogenesis and mouse ovarian development.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Daniele Missio, Alexandro Fritzen, Camila Cupper Vieira, Juliana Germano Ferst, Mariani Farias Fiorenza, Leonardo Guedes de Andrade, Bento Martins de Menezes, Monique Tomazele Rovani, Bernardo Gazieira Gasperin, Paulo Bayard Dias Goncalves, Rogerio Ferreira
Summary: The increase in intrafollicular concentrations of BHBA affects follicular growth, but it does not compromise the ovulatory cascade and cellular homeostasis in bovine granulosa cells.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Daniele Missio, Alexandro Fritzen, Camila Cupper Vieira, Juliana Germano Ferst, Mariani Farias Fiorenza, Leonardo Guedes de Andrade, Bento Martins de Menezes Bisneto, Monique Tomazele Rovani, Bernardo Gazieira Gasperin, Paulo Bayard Dias Goncalves, Rogerio Ferreira
Summary: Metabolic stress conditions caused by negative energy balance can reduce fertility in cows. Injection of beta-hydroxybutyrate (BHBA) affects follicle growth in cattle, but does not compromise ovulatory mechanisms and cellular homeostasis.
ANIMAL REPRODUCTION SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Camilla H. K. Hughes, Bruce D. Murphy
Summary: The development of ovarian follicles to ovulation is essential for fertility, with nuclear receptors playing key roles in regulating granulosa cell proliferation, hormone synthesis, and final stages of follicular growth. LRH-1 is a crucial nuclear receptor in these processes, and other receptors like ESR2 and AR also play important roles.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Reproductive Biology
Corie M. Owen, Laurinda A. Jaffe
Summary: The present study reveals that LH stimulation induces granulosa cell ingression in the mural granulosa layer, leading to changes in the follicular structure. This cellular ingression begins shortly after the LH surge and continues until ovulation, with an increase in the proportion of LH receptor-expressing cells in the inner half of the mural granulosa layer. Additionally, the study also observes changes in cell shape and follicular structure during this process.
BIOLOGY OF REPRODUCTION
(2023)
Article
Pharmacology & Pharmacy
Yinda Qiu, Bo Fang, Nguyen Thi Thanh Thuy, Aoding Li, Hee Min Yoo, Xiaohui Zheng, Namki Cho
Summary: Narciclasine inhibits proliferation and migration of esophageal cancer cells through FAK/JNK and p38 pathways, accompanied by DNA damage, cell cycle arrest, and apoptosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Cell Biology
Iman Dilower, Asef J. Niloy, Vishnu Kumar, Ayushi Kothari, Eun Bee Lee, M. A. Karim Rumi
Summary: The hedgehog (HH) signaling pathway plays a crucial role in development and reproductive functions, involving various molecules, receptors, and transcription factors. It has significant roles in the central nervous system, reproductive organs, and skeletal development, as well as essential physiological functions in adult testes and ovaries.
Article
Agriculture, Dairy & Animal Science
Xiaoxu Shen, Xiyu Zhao, Haorong He, Yao Zhang, Qing Zhu, Huadong Yin
Summary: SLC5A5 plays a crucial role in the development and function of chicken ovarian follicle granulosa cells, influencing cell proliferation, steroid hormone synthesis, and apoptosis. This discovery sheds light on the broader context of chicken ovarian follicle development and potential ways to improve egg-laying performance through molecular marker-assisted breeding technology.
Article
Endocrinology & Metabolism
Bo Pan, Xiaoshu Zhan, Julang Li
Summary: This study found that miR-574 expression decreases in porcine granulosa cells during follicular development, accompanied by an increase in phosphorylated ERK1/2. It was further discovered that miR-574 is involved in regulating estradiol production in granulosa cells through the TIMP3-pERK1/2 cascade.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Oncology
Yuxi Cheng, Juan Chen, Yuxin Shi, Xiaodan Fang, Zhangui Tang
Summary: The aim of this review is to summarize our studies on the signaling pathways involved in the development of oral cancer and several molecular targeting technologies combined with immune checkpoint therapies to provide new therapeutic strategies for oral squamous cell carcinoma. Oral squamous cell carcinoma accounts for 95% of human head and neck squamous cell carcinoma cases and has a poor prognosis. The mitogen-activated protein kinase (MAPK) signaling pathway is activated in over 50% of human oral cancer cases. We review the research progress on the MAPK signaling pathway and discuss its potential as a therapeutic strategy for oral squamous cell carcinoma.
Article
Cell Biology
Yuan Luo, Qi Yao
Summary: This study found that circRNA_0085315 plays an important role in colon cancer. It acts as a ceRNA to activate the MAPK signaling pathway through the miR-1200/MAP3K1 axis, promoting the progression of colon cancer.
Article
Chemistry, Multidisciplinary
Ainy Zehra, Hassan Alshemmari, Yasar N. Kavil, Abdul Majid Khan, Muhammad Zaffar Hashmi
Summary: The study focused on assessing the toxic effects of PCB70 and PCB75 on HeLa cells, showing PCB70 promotes cell proliferation at low doses and inhibits at high doses, while PCB75 causes membrane integrity deformities in a dose and time-dependent manner. The exposure to PCB congeners also induced the expression of ERK1/2 and JNK in a dose- and time-dependent manner in HeLa cells.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Oncology
Jian-Qiu Wang, Feng-Qin Yan, Li-Hui Wang, Wen-Juan Yin, Ting-Yu Chang, Jun-Ping Liu, Kou-Juey Wu
GENES CHROMOSOMES & CANCER
(2020)
Article
Biochemistry & Molecular Biology
Wei Liu, Xiuying Duan, Lingna Xu, Weina Shang, Jiayao Zhao, Liquan Wang, Jian-Chiuan Li, Chun-Hong Chen, Jun-Ping Liu, Chao Tong
CELL DEATH AND DIFFERENTIATION
(2020)
Review
Pharmacology & Pharmacy
Ning Liu, Xue-Hua Guo, Jun-Ping Liu, Yu-Sheng Cong
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2020)
Article
Pharmacology & Pharmacy
Zhiguo Wang, Jianfeng Li, Jun Liu, Lihui Wang, Yanhua Lu, Jun-Ping Liu
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2020)
Article
Medicine, General & Internal
Cheng-Xian Guo, Li He, Ji-Ye Yin, Xiang-Guang Meng, Wei Tan, Guo-Ping Yang, Tao Bo, Jun-Ping Liu, Xin-Jian Lin, Xiang Chen
Article
Biochemistry & Molecular Biology
Zhaoying Yang, Xiaocui Zhao, Weina Shang, Yang Liu, Jun-Feng Ji, Jun-Ping Liu, Chao Tong
Summary: P5CS, an enzyme responsible for synthesizing pyrroline-5-carboxylate, plays a crucial role in proline and ornithine synthesis. Dysfunctional P5CS can lead to various human diseases, affecting proliferation rate, cellular stress sensitivity, and lipid-droplet accumulation. The study reveals the importance of P5CS in regulating lipid metabolism and mitochondrial respiratory complex organization, shedding light on its role in metabolic disorders.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Jun Liu, Jun-Ping Liu
Article
Biochemistry & Molecular Biology
Jun Liu, Xiaojing Hong, Chao-Ya Liang, Jun-Ping Liu
Article
Biology
Lingna Xu, Xi Wang, Jia Zhou, Yunyi Qiu, Weina Shang, Jun-Ping Liu, Liquan Wang, Chao Tong
Article
Cell Biology
Jun Liu, Xiaojing Hong, Lihui Wang, Chao-Ya Liang, Jun-Ping Liu
Summary: Telomere shortening leads to cellular senescence, and this study reveals a dual opposing control mechanism of sub-telomeric Y' element recombination by Sir3/4 and Rif1, regulating telomere length and cell senescence in yeast Saccharomyces cerevisiae. Sir4 repression of Y' element recombination is negatively regulated by Rif1, mediating senescence-evasion induced by Sir4 deficiency.
Review
Pharmacology & Pharmacy
Da-Yong Zhang, Michael J. Monteiro, Jun-Ping Liu, Wen-Yi Gu
Summary: CSCs, a small population of tumor cells with self-renewal and aberrant differentiation abilities, play crucial roles in tumor development and recurrence. Research indicates that CSCs can undergo cell senescence, allowing them to evade the cytotoxic effects of cancer therapy.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2021)
Article
Cell Biology
Xiuying Duan, Lingna Xu, Yawen Li, Lijun Jia, Wei Liu, Wenxia Shao, Vafa Bayat, Weina Shang, Liquan Wang, Jun-Ping Liu, Chao Tong
Summary: Through experiments involving overexpression and mutations, it was found that dTBC1D22 interacts with Rab40, regulating lipid droplet accumulation and mobilization, dependent on the distribution of Lamp1. The impact of Rab40 on lipophagy and Golgi function may lead to lipid homeostasis imbalance.
Article
Cell Biology
Kexiong Zhang, Lihui Wang, Xiaojing Hong, Hao Chen, Yao Shi, Yingying Liu, Jun Liu, Jun-Ping Liu
Summary: The Telomerase RNA component (TERC) knockout leads to p53-dependent premature replicative senescence and apoptosis of pulmonary stem cells, which can be significantly reduced by p53 deficiency. These findings demonstrate the association between TERC deficiency-induced telomere DDR and pulmonary aging.
Review
Cell Biology
Xiaojing Hong, Lihui Wang, Kexiong Zhang, Jun Liu, Jun-Ping Liu
Summary: Pulmonary senescence, accelerated by unresolved DNA damage response, increases susceptibility to pulmonary fibrosis. Recent studies have shown that SARS-Cov-2 viral infection induces acute pulmonary epithelial senescence followed by fibrosis, although the underlying mechanism is unclear. This study explores the roles of alveolar epithelial stem cell senescence and senescence-associated differentiation disorders in pulmonary fibrosis, and investigates the mechanisms involved in mediating and preventing fibrogenic crisis. The TGF-beta signaling pathway mediates alveolar epithelial stem cell senescence through suppression of the telomerase reverse transcriptase gene, while telomere uncapping caused by stress-induced telomeric shelterin protein TPP1 degradation mediates DNA damage response, pulmonary senescence, and fibrosis. Targeted intervention of cellular senescence disrupts pulmonary remodeling and fibrosis by clearing senescent cells or preventing senescence using telomere dysfunction inhibitor (TELODIN). Studies suggest that senescence-associated differentiation disorders can be reprogrammed and reversed by inhibiting stem cell replicative senescence in pulmonary fibrosis, providing a framework for targeted intervention of the molecular mechanisms of alveolar stem cell senescence and pulmonary fibrosis.
Article
Biochemical Research Methods
Lihui Wang, Zhiguo Wang, Jun -Ping Liu
Summary: The study utilized the TIF assay to screen synthetic peptides to prevent telomere dysfunctions caused by environmental radiation, demonstrating a potential intervening therapeutic drug candidate. This work presents a novel approach for identifying telomere dysfunction inhibitors.
