Review
Cell Biology
Vicente Barrios, Julie A. Chowen, Alvaro Martin-Rivada, Santiago Guerra-Cantera, Jesus Pozo, Shoshana Yakar, Ron G. Rosenfeld, Luis A. Perez-Jurado, Juan Suarez, Jesus Argente
Summary: The GH/IGF axis is crucial in human growth, with recent research focusing on the role of the PAPP-A2 gene mutations in affecting physiology. Understanding the implications of PAPP-A2 can provide insights for controlling growth processes.
Review
Endocrinology & Metabolism
Claus Oxvig, Cheryl A. Conover
Summary: PAPP-A and PAPP-A2 are specific proteolytic enzymes involved in IGF signaling regulation by cleaving IGFBPs and increasing IGF bioactivity. This molecular network plays a significant role in the IGF system, but its precise operation needs further investigation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biochemical Research Methods
Shuvashis Dey, Kevin M. Koo, Emtiaz Ahmed, Matt Trau
Summary: The analysis of secreted protein biomarkers through a nanophotonic immunoarray sensor enables high-throughput, sensitive, and portable detection of sPD-L1. The sensor offers advantages such as surface-enhanced Raman scattering analysis, improved sensitivity compared to ELISA, and compatibility with handheld detection. The performance evaluation demonstrated successful quantitative detection of sPD-L1 in contrived human plasma samples.
Article
Nanoscience & Nanotechnology
Nan-Fu Chiu, Ming-Jung Tai, Devi Taufiq Nurrohman, Ting-Li Lin, Ying-Hao Wang, Chen-Yu Chen
Summary: A highly sensitive SPR biosensor using carboxyl-MoS2 film was developed for evaluating PAPP-A2 levels in maternal serum samples for Down's syndrome screening. The technology showed acceptable accuracy and recovery rates, suggesting potential advantages over conventional ELISA.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Shreyasee Das, Nele Dewit, Dirk Jacobs, Yolande A. L. Pijnenburg, Sjors G. J. G. In't Veld, Salome Coppens, Milena Quaglia, Christophe Hirtz, Charlotte E. Teunissen, Eugeen Vanmechelen
Summary: This study describes a novel Nf-L enzyme-linked immunosorbent assay (ELISA) with well-defined specificity and epitopes, which can accurately measure Nf-L analytes in cerebrospinal fluid and discriminate different diseases in clinical diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Lee D. Gibbs, Kelsey Mansheim, Sayantan Maji, Rajesh Nandy, Cheryl M. Lewis, Jamboor K. Vishwanatha, Pankaj Chaudhary
Summary: Annexin A2 (AnxA2) is overexpressed in tumor tissues and serum samples of breast cancer patients, especially in the TNBC subtype, where its expression is associated with poor prognosis. The phosphorylation of AnxA2 at tyrosine 23 appears to be a critical step for its secretion from TNBC cells and could serve as a potential diagnostic biomarker in TNBC patients.
Article
Medicine, General & Internal
Abirami Moorthy, Divyambika Catakapatri Venugopal, Vidyarani Shyamsundar, Yasasve Madhavan, Soundharya Ravindran, Mehanathan Kuppuloganathan, Arvind Krishnamurthy, Sathasivasubramanian Sankarapandian, Vani Ganapathy, Vijayalakshmi Ramshankar
Summary: Oral Submucous Fibrosis (OSMF) is a chronic disease more commonly found in the South-East Asian population and has the potential to progress to oral malignant disorders. The study indicates that EGFR overexpression is significantly expressed in OSMF and OSCC, serving as a potential diagnostic biomarker.
Article
Immunology
Harriet N. Garlant, Kalaiarasan Ellappan, Matthew Hewitt, Prem Perumal, Simon Pekeleke, Nadina Wand, Jo Southern, Saka Vinod Kumar, Harish Belgode, Ibrahim Abubakar, Sanjeev Sinha, Seshadri Vasan, Noyal Mariya Joseph, Karen E. Kempsell
Summary: Tuberculosis (TB) is a major global health crisis and the leading cause of death among infectious diseases. The diagnosis of TB is challenging and hinders its control and eradication. This study evaluates the diagnostic performance of nine biomarkers as protein targets in TB diagnosis. Some of these biomarkers show great potential for developing diagnostic tests for TB.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Hee-Sung Ahn, Jung Yoon Ho, Jiyoung Yu, Jeonghun Yeom, Sanha Lee, Soo Young Hur, Yuyeon Jung, Kyunggon Kim, Youn Jin Choi
Summary: Most hereditary ovarian cancer is associated with BRCA1/2 variants, and plasma protein biomarkers, specifically SPARC and THBS1, may indicate increased risk of developing ovarian cancer in BRCA1/2 carriers. Monitoring these protein concentrations could potentially help in deciding whether to undergo oophorectomy for disease prevention.
Article
Clinical Neurology
Feng Zhang, Guangshun Hou, Guangjian Hou, Congan Wang, Bin Shi, Yuekun Zheng
Summary: This study found that serum irisin levels were significantly lower in VD patients compared to controls, and irisin was positively correlated with cognitive function. Serum irisin levels can serve as a biological marker for cognitive impairment in VD patients, even after adjustment for risk factors.
FRONTIERS IN NEUROLOGY
(2021)
Article
Chemistry, Analytical
Isabelle C. Samper, Catherine J. McMahon, Melissa S. Schenkel, Kaylee M. Clark, Wisarut Khamcharoen, Loran B. R. Anderson, James S. Terry, Emily N. Gallichotte, Gregory D. Ebel, Brian J. Geiss, David S. Dandy, Charles S. Henry
Summary: A highly sensitive electrochemical immunoassay has been developed for quantitatively detecting the presence of SARS-CoV-2 virus in patient nasopharyngeal samples. The assay demonstrates high specificity and sensitivity, meeting the criteria for POC COVID-19 diagnostic tests set by the World Health Organization.
ANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Meiyan Luo, Qian Zhang, Yingchun Hu, Changfeng Sun, Yunjian Sheng, Cunliang Deng
Summary: This study identified differentially expressed proteins in septic patients' plasma using DIA and ELISAs, discovering their involvement in cell movement, stress response, cell proliferation, and immune response. LGALS3BP was highlighted as a potential plasma biomarker with diagnostic and prognostic value in sepsis.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Oncology
Klaus Felix, Kazufumi Honda, Kengo Nagashima, Ayumi Kashiro, Keiko Takeuchi, Takashi Kobayashi, Sascha Hinterkopf, Matthias M. Gaida, Hien Dang, Niall Brindl, Joerg Kaiser, Markus W. Buechler, Oliver Strobel
Summary: This study found that serum apoA2-i has potential clinical value in risk stratification of IPMN and associated cancer. It showed higher accuracy and sensitivity in diagnosing IPMN-associated cancer compared to the routine biomarker CA 19-9. Further validation in larger and independent international cohort studies is needed for clinical application.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Suman Dutta, Simon Hornung, Hash Brown Taha, Karl Biggs, Ibrar Siddique, Lea M. Chamoun, Hedieh Shahpasand-Kroner, Carter Lantz, Marcos Herrera-Vaquero, Nadia Stefanova, Joseph A. Loo, Gal Bitan
Summary: A new method for sensitive detection of pS129-alpha-syn has been developed, which can be applied to various biological samples. This method provides a valuable tool for discovering and monitoring neurodegenerative diseases associated with abnormal accumulation of synaptic proteins.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Microbiology
Yuan Ren, Lilan Hao, Juntao Liu, Pei Wang, Qiuxia Ding, Chen Chen, Yingna Song
Summary: In this study, the changes in gut microbiota composition in pregnant women with and without pregestational type 2 diabetes mellitus (PGDM) were investigated. The results showed that the gut microbiota in the PGDM group decreased as the pregnancies progressed, and certain bacteria taxa were enriched in the PGDM group. Moreover, the gut microbiota in the PGDM group was correlated with blood glucose levels during pregnancy. This study is important for understanding the relationship between PGDM and gut microbiota.
Article
Gastroenterology & Hepatology
Susanne Haas, Anette Hojer Mikkelsen, Camilla Jensenius Skovhus Kronborg, Birthe T. Oggesen, Pia F. Moller, Janne Fassov, Nina Abild Frederiksen, Marianne Krogsgaard, Charlotte Graugaard-Jensen, Lise Venzel, Peter Christensen, Katrine Jossing Emmertsen
Summary: This study examines the extent of late treatment-related sequelae in colorectal cancer survivors and provides evidence-based management strategies, aiming to facilitate identification and treatment of these conditions. Results show common symptoms and provide recommendations and strategies for their management.
COLORECTAL DISEASE
(2023)
Article
Oncology
Anna Cecilie Lef, Eva Serup-Hansen, Katrine Smedegaard Storm, Karen Lycke Wind, Camilla Kronborg, Karen-Lise Garm Spindler
Summary: The purpose of this study was to report patient-reported outcome (PRO) and National Cancer Institute-Common Terminology Criteria for Adverse Events (NCI-CTCAE) data for patients with squamous cell carcinoma of the anus (SCCA) treated with modern radiation therapy (RT) 1 year after treatment. The study found that functional mean scores were comparable to a normal population cohort, and there were minor improvements in global health status/quality of life, emotional functioning, insomnia, and appetite loss. Bowel and bladder symptoms showed minor improvements or stable conditions, while some symptoms worsened moderately.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Review
Endocrinology & Metabolism
Claus Oxvig, Cheryl A. Conover
Summary: PAPP-A and PAPP-A2 are specific proteolytic enzymes involved in IGF signaling regulation by cleaving IGFBPs and increasing IGF bioactivity. This molecular network plays a significant role in the IGF system, but its precise operation needs further investigation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Oncology
Karen Lycke Wind, Karen-Lise Garm Spindler, Christina Maria Lutz, Lars Nyvang, Camilla Kronborg
Summary: This study aimed to estimate the dose of loco-regional recurrences (LRRs) in anal cancer using a point of origin-based method. It was found that the majority of LRRs were located within the high dose volume, indicating radioresistance as the main cause of recurrence in anal cancer.
PHYSICS & IMAGING IN RADIATION ONCOLOGY
(2023)
Review
Endocrinology & Metabolism
Cheryl A. Conover, Claus Oxvig
Summary: PAPP-A is a placental protein that was initially discovered in pregnant women and later found to be a metalloproteinase expressed by nonplacental cells. It regulates local insulin-like growth factor (IGF) activity by cleaving high-affinity IGF binding proteins (IGFBPs). This proteolytic regulation is important for cell proliferation, differentiation, migration, and survival in normal and cancer cells.
Article
Chemistry, Applied
Simon Gregersen Echers, Ali Jafarpour, Betul Yesiltas, Pedro J. Garcia-Moreno, Mathias Greve-Poulsen, Dennis K. Hansen, Charlotte Jacobsen, Michael Toft Overgaard, Egon Bech Hansen
Summary: In this study, a novel workflow was presented to produce a potato protein hydrolysate with improved emulsifying and foaming properties. The targeted hydrolysis design was facilitated using quantitative proteomics and bioinformatic prediction. This interdisciplinary approach shows promise for the production of functional hydrolysates from alternative protein sources.
FOOD HYDROCOLLOIDS
(2023)
Article
Cardiac & Cardiovascular Systems
Morten Krogh Christiansen, Kasper Kjaer-Sorensen, Natacha C. Clavsen, Sven Dittmann, Maja Fuhlendorff Jensen, Halvor Osterby Guldbrandsen, Lisbeth Norum Pedersen, Rikke Hasle Sorensen, Dorte Launholt Lildballe, Klara Mueller, Patrick Mueller, Kira Vogel, Boris Rudic, Martin Borggrefe, Claus Oxvig, Christian Aalkjaer, Eric Schulze-Bahr, Vladimir Matchkov, Henning Bundgaard, Henrik Kjaerulf Jensen
Summary: This study investigated the prevalence of potential disease-causing variants in SQTS patients, with a focus on the SLC4A3 gene. Results showed that nonsynonymous variants in SLC4A3 were the most common cause of SQTS, highlighting the importance of including SLC4A3 in the genetic screening of SQTS or sudden cardiac death patients.
Article
Gastroenterology & Hepatology
Yun Huang, Sara E. Stinson, Helene Baek Juel, Morten A. V. Lund, Louise Aas Holm, Cilius E. Fonvig, Trine Nielsen, Niels Grarup, Oluf Pedersen, Michael Christiansen, Elizaveta Chabanova, Henrik S. Thomsen, Aleksander Krag, Stefan Stender, Jens-Christian Holm, Torben Hansen
Summary: This study investigated the effects of eight previously reported genome-wide significant steatogenic variants on liver and cardiometabolic traits in children and adolescents. The results showed that these variants were associated with higher liver fat and distinct patterns of plasma lipids. The genetic risk score (GRS) based on these variants was also associated with higher prevalence of hepatic steatosis and had potential clinical utility for risk stratification.
LIVER INTERNATIONAL
(2023)
Review
Multidisciplinary Sciences
Paula L. Hedley, Christian M. Hagen, Casper Wilstrup, Michael Christiansen
Summary: Early pregnancy screening is important for preventing pre-eclampsia. This study aims to develop risk prediction algorithms through the application of artificial intelligence and machine learning. A systematic review and assessment of studies on the application of AI/ML in early pregnancy screening will be conducted.
Article
Orthopedics
Paula L. Hedley, Ulrik Lausten-Thomsen, Kristin M. Conway, Klaus Hindso, Paul A. Romitti, Michael Christiansen
Summary: This study used publicly available data to investigate the prevalence and co-occurring anomalies of clubfoot in Denmark from 1994 to 2021. The findings showed that the prevalence of nonsyndromic clubfoot remained stable during the study period, and the reduction in maternal smoking rates did not significantly impact the prevalence. These results support the suspected multifactorial etiopathogenesis of clubfoot and provide a valuable population-based resource for future investigations.
BMC MUSCULOSKELETAL DISORDERS
(2023)
Article
Cardiac & Cardiovascular Systems
Lia Crotti, Carla Spazzolini, Mette Nyegaard, Michael T. Overgaard, Maria-Christina Kotta, Federica Dagradi, Luca Sala, Takeshi Aiba, Mark D. Ayers, Anwar Baban, Julien Barc, Cheyenne M. Beach, Elijah R. Behr, J. Martijn Bos, Marina Cerrone, Peter Covi, Bettina Cuneo, Isabelle Denjoy, Birgit Donner, Adrienne Elbert, Hakan Eliasson, Susan P. Etheridge, Megumi Fukuyama, Francesca Girolami, Robert Hamilton, Minoru Horie, Maria Iascone, Juan Jimenez Jaimez, Henrik Kjaerulf Jensen, Prince J. Kannankeril, Juan P. Kaski, Naomasa Makita, Carmen Munoz-Esparza, Hans H. Odland, Seiko Ohno, John Papagiannis, Alessandra Pia Porretta, Christopher Prandstetter, Vincent Probst, Tomas Robyns, Eric Rosenthal, Ferran Roses-Noguer, Nicole Sekarski, Anoop Singh, Georgia Spentzou, Fridrike Stute, Jacob Tfelt-Hansen, Jan Till, Kathryn E. Tobert, Jeffrey M. Vinocur, Gregory Webster, Arthur A. M. Wilde, Cordula M. Wolf, Michael J. Ackerman, Peter J. Schwartz
Summary: The International Calmodulinopathy Registry (ICalmR) is an international collaborative study that aims to understand the clinical manifestations and molecular mechanisms of calmodulinopathy caused by CALM gene mutations. The registry has enrolled 140 patients, mainly presenting with CALM-LQTS and CALM-CPVT, with some patients also exhibiting neurological symptoms. The study shows a reduced frequency and severity of cardiac events in recent years.
EUROPEAN HEART JOURNAL
(2023)
Article
Biochemical Research Methods
Rasmus Hejlesen, Kasper Kjaer-Sorensen, Angela Fago, Claus Oxvig
Summary: By using the CRISPR/Cas system, three distinct zebrafish mb knockout lines were generated, all of which did not exhibit significant phenotypic changes during development. The validated knockout line, mb(Auzf13.2), showed no signs of genetic compensation or off-target effects in adult zebrafish, suggesting promise for investigating the biological role of Mb.
TRANSGENIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Anne Skakkebaek, Kasper Kjaer-Sorensen, Vladimir V. Matchkov, Lise-Lotte Christensen, Jesper Just, Cagla Comert, Niels Holmark Andersen, Claus Oxvig, Claus Hojbjerg Gravholt
Summary: The genetic basis of the QT interval, which represents the time for heartbeat repolarization, is not fully understood. This study explores the contribution of the pseudoautosomal gene SLC25A6 to QT interval variation using human participants and zebrafish models. The results show that higher expression of SLC25A6 is associated with shorter QTc intervals, and pharmacological modulation of K-ATP channels can restore the interval duration. This indicates that SLC25A6 plays a role in QT interval variation.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Pernille Bogetofte Thomasen, Alena Salasova, Kasper Kjaer-Sorensen, Lucie Woloszczukova, Josef Lavicky, Hande Login, Jeppe Tranberg-Jensen, Sergio Almeida, Sander Beel, Michaela Kavkova, Per Qvist, Mads Kjolby, Peter Lund Ovesen, Stella Nolte, Benedicte Vestergaard, Andreea-Cornelia Udrea, Lene Niemann Nejsum, Moses Chao, Philip Van Damme, Jan Krivanek, Jeremy Dasen, Claus Oxvig, Anders Nykjaer
Summary: SorCS2 is a receptor for PGRN that plays an important role in MN diversification and axon outgrowth, as well as affecting neuromuscular junction morphology and fish motility. Deficiency of SorCS2 disrupts cell-fate decisions of brachial MNs and leads to innervation deficits of posterior nerves. In addition, SorCS2 knockout mice exhibit slower motor nerve regeneration. The interaction between primitive macrophages expressing high levels of PGRN and SorCS2-positive motor axons is crucial for axon pathfinding.
Article
Physiology
Arimantas Lionikas, Ana I. Hernandez Cordero, Audrius Kilikevicius, Andrew M. Carroll, Guy S. Bewick, Lutz Bunger, Aivaras Ratkevicius, Lora K. Heisler, Mette Harboe, Claus Oxvig
Summary: This study suggests that STC2 inhibits muscle growth by inhibiting the activity of PAPP-A, a protease that promotes muscle growth through the IGF axis. Mice lacking STC2 showed increased muscle mass and larger muscle fibers. Functional overload increased muscle mass and the levels of IGF axis components, including IGF1, IGF2, IGFBP-4, PAPP-A, and STC2.
PHYSIOLOGICAL REPORTS
(2023)
