期刊
MOLECULAR GENETICS AND GENOMICS
卷 285, 期 5, 页码 403-413出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00438-011-0614-3
关键词
Coliphage HK022; Integrase; Site-specific recombination; Human embryonic kidney cell culture
资金
- Leon Reich Scholarship Fund
- BARD [MB-8713-08]
- United States-Israel Binational Agricultural Research and Development Fund
- G.I.F. [1062/2008]
- German Israeli Foundation for Scientific Research and Development
The integrase encoded by the lambdoid phage HK022 (Int-HK022) resembles its coliphage counterpart (Int-lambda) in the roles of the cognate DNA arm binding sites and in controlling the direction of the reaction. We show here that within mammalian cells, Int-HK022 does not exhibit such a control. Rather, Int-HK022 recombined between all ten possible pairwise att site combinations, including attB x attB that was more effective than the conventional integrative attP x attB reaction. We further show that Int-HK022 depends on the accessory integration host factor (IHF) protein considerably less than Int-lambda and exhibits stronger binding affinity to the att core. These differences explain why wild-type Int-HK022 is active in mammalian cells whereas Int-lambda is active there only as an IHF-independent mutant.
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