Article
Biotechnology & Applied Microbiology
Haiyang Wang, Lulu Zhai, Shouwen Wang, Botian Zheng, Honglu Hu, Xuyan Li, Shaomin Bian
Summary: In this study, the roles of MYB family in blueberry in response to anthocyanin biosynthesis were explored. A total of 437 unique MYB sequences were identified and characterized in blueberry, and their transcriptional patterns during fruit development and fruit tissues related to anthocyanin biosynthesis were investigated. The results provide valuable insights into the functional roles of VcMYBs and their regulatory mechanisms for anthocyanin production and accumulation in blueberry.
Article
Agronomy
Chan Bi, Chaoxiong Wei, Shuai Tian, Jinghui Li, Yufeng Zhang, Jun Ma, Mingshan You
Summary: The purple color of wheat grain is controlled by two regulatory genes, TaPpm1 and TaPpb1, which regulate the accumulation of anthocyanins in the pericarp. Through crosses between nonpurple-grained DH lines and an EMS-induced mutant, the purple character was successfully recovered in all the progeny, although the seed color was visually lighter than that of the purple-grained parent. The expression patterns of the regulatory genes and structural genes involved in anthocyanin biosynthesis were found to contribute to the dosage effect of wheat purple grain color.
Article
Multidisciplinary Sciences
Shuangping Heng, Changbin Gao, Mengdi Cui, Jing Fu, Sujing Ren, Kaiyun Xin, Congan He, Aihua Wang, Liping Song, Liguang Tang, Bincai Wang, Xueli Zhang
Summary: This study investigated the metabolites and gene expression patterns involved in dark red taproot color formation in radish. The findings suggest that pelargonin and pelargonidin 3-O-beta-D-glucoside are the main pigments responsible for the coloration, and most genes involved in anthocyanin biosynthesis are up-regulated in dark red taproots. The coexpression of RsMYB1 and RsMYB2 transcription factors may contribute to the formation of dark red color. This work provides valuable insights for breeding anthocyanin-rich radish.
Article
Biochemistry & Molecular Biology
Shaozhe Wen, Ning Li, Shuhui Song, Ning Liu, Yunhua Ding
Summary: Due to the high content of anthocyanin, broccoli varieties with purple curds have gained popularity, but the genetic mechanisms of anthocyanin biosynthesis pathways are largely unknown. A study bred near-isogenic lines with green and purple curds, and found that genes related to anthocyanin biosynthesis were significantly upregulated in the purple curds. Metabolic analysis also showed differential accumulation of metabolites, particularly anthocyanins, in the purple curds. The study identified specific genes and metabolic factors contributing to the purple curds, which could aid in breeding purple broccoli varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Yaolei Mi, Ruikun He, Huihua Wan, Xiangxiao Meng, Di Liu, Wenjun Huang, Yanjun Zhang, Zubaida Yousaf, Hongwen Huang, Shilin Chen, Ying Wang, Wei Sun
Summary: In this study, the anthocyanidins of eight different Epimedium species with different floral pigmentation phenotypes were analyzed. The expression levels of catalytic enzyme genes DFR and ANS were significantly decreased in four species showing loss of floral pigmentation. Complementation and overexpression experiments confirmed the functional role of these genes at the protein level.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Qing Xia, Yang Shui, Hui Zhi, Aamir Ali, Zhenping Yang, Zhiqiang Gao
Summary: Through transcriptomics and metabolomics, it was found that selenium biofortification increases the concentrations of selenium, anthocyanins, chlorophyll a and b, and carotenoids in colored-grain wheat. Upregulation of genes related to the biosynthesis of anthocyanins, phenylpropanoids, and flavonoids after selenium treatment leads to the accumulation of anthocyanin metabolites in colored-grain wheat.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agronomy
Yongjun He, Shaohang Li, Yanxiao Dong, Xintong Zhang, Dalu Li, Yang Liu, Huoying Chen
Summary: Through the study of the specific gene SmFTSH10 in photosensitive and non-photosensitive eggplants, this research provides new insights into the molecular mechanisms of anthocyanin biosynthesis in non-photosensitive eggplants.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Van Giap Do, Youngsuk Lee, Jeong-Hee Kim, Young-Soon Kwon, Jong-Taek Park, Sangjin Yang, Juhyeon Park, Nay Myo Win, Seonae Kim
Summary: The accumulation of anthocyanin is responsible for the coloration of apple fruit, and this accumulation depends on the expression of genes related to anthocyanin biosynthesis. Light plays a role in inducing fruit coloration by regulating genes involved in anthocyanin biosynthesis. This study investigates the effects of light and genetic factors on the coloration and accumulation of anthocyanin in apple fruits. The findings suggest that light, together with the overexpression of genes in the anthocyanin biosynthesis pathway, induces the coloration and accumulation of anthocyanin in apple fruits by upregulating the expression of these genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luhong Leng, Xiaoni Zhang, Weichao Liu, Zhiqiang Wu
Summary: Carnations are popular ornamental flowers with varied flower colors, which are mainly due to the accumulation of flavonoid compounds. MYB and bHLH transcription factors regulate the expression of anthocyanin biosynthetic genes. This study identified 106 MYB and 125 bHLH genes in the carnation genome and revealed key genes (DcaMYB13 and DcabHLH125) responsible for the formation of red petals in carnations. The findings provide valuable information for further studies on the regulation of anthocyanin biosynthesis in carnations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Gautam Saripalli, Laxman Adhikari, Cameron Amos, Ashraf Kibriya, Hanin Ibrahim Ahmed, Matthias Heuberger, John Raupp, Naveenkumar Athiyannan, Thomas Wicker, Michael Abrouk, Sydney Wallace, Seyedali Hosseinirad, Parveen Chhuneja, Janelle Livesay, Nidhi Rawat, Simon G. Krattinger, Jesse Poland, Vijay Tiwari
Summary: This study utilized genomic advancements to map agronomic traits in einkorn wheat, resulting in the identification of candidate genes and a domestication locus. This resource can be utilized to improve wheat cultivars and explore agronomically important traits.
COMMUNICATIONS BIOLOGY
(2023)
Article
Agronomy
Zhaofang Chen, Honghong Deng, Bo Xiong, Sichen Li, Liancong Yang, Youting Yang, Shengjia Huang, Liping Tan, Guochao Sun, Zhihui Wang
Summary: This study evaluated the effect of three commonly used rootstocks on the features, color index, physicochemical parameters, anthocyanin accumulation, gene expression, and enzyme activity of blood orange during fruit development and ripening. Results showed that the Trifoliate orange rootstock promotes a high anthocyanin accumulation in blood orange, with a strong correlation between anthocyanin content and gene expression/enzyme activity.
Article
Plant Sciences
Xiaoning Luo, Daoyang Sun, Shu Wang, Sha Luo, Yaqi Fu, Lixin Niu, Qianqian Shi, Yanlong Zhang
Summary: The study revealed differences in pigment content and gene expression levels between yellow and purple-red tree peony cultivars, with the yellow cultivar showing higher levels of yellow pigments and flavonoids in the S3 stage, as well as upregulation of transcription factor genes related to yellow pigmentation.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Irina G. Adonina, Andrey B. Shcherban, Maremyana V. Zorina, Sabina P. Mehdiyeva, Ekaterina M. Timonova, Elena A. Salina
Summary: This article studied three triticale-wheat hybrid lines with differences in vaviloid spike branching. Molecular genetic analysis revealed a deletion of the wheat domestication gene Q in the studied hybrid lines.
Article
Horticulture
Yaqiong Wu, Tianyu Han, Lianfei Lyu, Weilin Li, Wenlong Wu
Summary: Anthocyanins are important secondary metabolites in plants, which determine the color of flowers and fruits. They have various nutritional and medicinal functions, such as antioxidant, anticancer, and cardiovascular disease prevention. This review discusses the biosynthesis pathway of plant anthocyanins, the key structural genes involved, the impact of transcription factors and microRNAs, the influence of environmental factors, and the effects of six major exogenous hormones on anthocyanin synthesis and metabolism. Future research directions and trends in anthocyanins are also considered to provide a reference for future studies and applications.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Maria-Dolores Rey, Carmen Ramirez, Azahara C. Martin
Summary: The study reports the production of new hybrids formed by genomes from three different genera, with research on meiotic behavior showing multiple chromosome associations.
High levels of crossover formation in (HDR)-D-ch suggest the possibility of meiotic recombination between different genomes.
Successful duplication of the ABH(ch)R genome and characterization of several amphiploids indicate the potential for recombination between genera of three economically important crops.
Article
Plant Sciences
S. Mursalimov, A. Glagoleva, E. Khlestkina, O. Shoeva
Summary: The study discovered that melanin synthesis in barley seeds is controlled by two genes, Blp1 and Alm1, with Blp1 regulating melanin synthesis and Alm1 controlling chlorophyll synthesis. Although different genetic systems are responsible for these traits, they are localized in the same organelle. Cytological analysis revealed that husk cells have more developed internal structures, and melanin biosynthesis may depend on the presence of plastids with well-developed internal membranes.
Article
Agronomy
Anastasiya Glagoleva, Tatjana Kukoeva, Sergey Mursalimov, Elena Khlestkina, Olesya Shoeva
Summary: This study explored the effects of combined accumulation of anthocyanins and melanins in barley, and found that their presence positively influences the color and anthocyanin content of the grain. Additionally, the presence of pigments does not decrease plant productivity. These findings will contribute to the understanding of genetic mechanisms underlying pigment biosynthesis and breeding strategies for enriching barley varieties with polyphenols.
Article
Plant Sciences
Anastasiia Y. Glagoleva, Alexander V. Vikhorev, Nikolay A. Shmakov, Sergey V. Morozov, Elena I. Chernyak, Gennady V. Vasiliev, Natalia V. Shatskaya, Elena K. Khlestkina, Olesya Y. Shoeva
Summary: The current study reveals the features of the phenylpropanoid biosynthesis pathway functioning in melanin-accumulating barley grains. It shows that the pathway is redirected during melanin synthesis, leading to unique transcriptomic patterns and changes in phenylpropanoid metabolite concentrations. This study provides valuable insights into the genetics and metabolism of pigmentation in barley grains.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Galina Smolikova, Ksenia Strygina, Ekaterina Krylova, Aleksander Vikhorev, Tatiana Bilova, Andrej Frolov, Elena Khlestkina, Sergei Medvedev
Summary: The transition from seed to seedling is a crucial step in the plant life cycle. In this study, the authors investigated the changes in water and oxidative status, as well as gene expression, during this transition in Pisum sativum seeds. They found that the accumulation of ROS and the shift in water and oxidative status were correlated with the loss of desiccation tolerance. RNA sequencing analysis revealed differentially expressed genes related to metabolism, photosynthesis, cell wall biosynthesis, redox status, and stress responses. The findings suggest that specific genes, such as LTI65, LTP4, and HVA22E, may play a crucial role in the desiccation tolerance loss during the transition.
Editorial Material
Biochemistry & Molecular Biology
Elena Khlestkina, Yuri Shavrukov
Article
Plant Sciences
Elena Antonova, Nadezhda S. Shimalina, Anna M. Korotkova, Ekaterina Kolosovskaya, Sophia Gerasimova, Elena K. Khlestkina
Summary: The genes NUD and WIN1 play a regulatory role in cuticle organization in barley. Mutations in these genes may affect plant adaptation mechanisms and lead to alterations in root system and leaf morphology and color.
Article
Biochemistry & Molecular Biology
B. R. Kuluev, E. V. Mikhailova, A. R. Kuluev, A. A. Galimova, E. A. Zaikina, E. K. Khlestkina
Summary: This review discusses recent advances in genomic editing of cultivated plants in the Triticeae tribe, with a focus on bread wheat and barley. It also examines the tools and methods commonly used in this field, as well as approaches to overcome the low regenerative capacity observed in Triticeae plants.
Article
Genetics & Heredity
K. Strygina, A. G. Elatskova, Yu A. Elatskov, G. A. Tekhanovich, E. K. Khlestkina
Summary: Studying the genes related to dwarf watermelon plants is crucial for accelerating selection processes. By analyzing the unique VIR collection of melon crops and selection forms at the Kuban experimental station of VIR, potentially new genes determining dwarfism in watermelon were identified.
RUSSIAN JOURNAL OF GENETICS
(2022)
Article
Agronomy
Elena Gordeeva, Olesya Shoeva, Sergey Mursalimov, Irina Adonina, Elena Khlestkina
Summary: This study successfully created black-grained substitution lines in the genetic background of bread wheat using marker-assisted technology, allowing for the simultaneous accumulation of anthocyanins in the aleurone and pericarp layers with a high total anthocyanin content. Although chromosome substitution had a positive effect on the main spike length, it negatively affected other yield-related traits.
Article
Agriculture, Multidisciplinary
Olesya Yu. Shoeva, Marina A. Mukhanova, Shakhira Zakhrabekova, Mats Hansson
Summary: In this study, the molecular function of the Ant13 locus in barley was determined. It was found that Ant13 encodes a regulatory protein that plays a crucial role in the transcriptional activation of structural genes involved in flavonoid biosynthesis. Additionally, the mutants deficient in the Ant13 locus exhibited decreased root and shoot growth and yield-related parameters.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Irina V. Rozanova, Yuriy N. Grigoriev, Vadim M. Efimov, Alexander V. Igoshin, Elena K. Khlestkina
Summary: This study conducted a genome-wide association study on 94 barley varieties and identified 64 SNP markers significantly associated with spike productivity traits. Among them, 23 SNP markers were validated and 14 markers on chromosomes 2H, 4H and 5H were suggested for marker-assisted breeding programs.
Article
Plant Sciences
Ekaterina A. Krylova, Aleksandra S. Mikhailova, Yulia N. Zinchenko, Irina N. Perchuk, Mayya P. Razgonova, Elena K. Khlestkina, Marina O. Burlyaeva
Summary: This study focused on the pigmentation of legumes and their connection to flavonoids. By using various methods and analyses, the relationship between the genes in the MYB cluster on chromosome 5 and anthocyanin pigmentation was explored. The results showed that there is a correlation between the color of the seed coat and the anthocyanin content in Vigna unguiculata.
Article
Biochemistry & Molecular Biology
Daria V. Mikhailova, Oksana G. Shevchenko, Denis A. Golubev, Elena Y. Platonova, Nadezhda V. Zemskaya, Olesya Yu. Shoeva, Elena I. Gordeeva, Sergey A. Patov, Mikhail V. Shaposhnikov, Elena K. Khlestkina, Alexey Moskalev
Summary: This study examines the antioxidant and geroprotective properties of wheat bran extracts with high anthocyanin levels. The results show that the extracts with anthocyanins have higher radical scavenging and membrane protective activities, and can extend the lifespan of Drosophila. However, other metabolites in wheat bran may also contribute to its antioxidant and geroprotective potential.