Article
Biochemistry & Molecular Biology
Cristina Cacheiro-Llaguno, Elena Hernandez-Subira, Manuel D. Diaz-Munoz, Manuel Fresno, Juan M. Serrador, Miguel A. Iniguez
Summary: This research investigates the inhibitory effect of dexamethasone on COX-2 transcriptional induction during T cell activation, revealing the immunomodulatory properties of glucocorticoids in human T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yue Leng, Yonghai Sun, Chengyu Lv, Zhuolin Li, Cuiping Yuan, Jie Zhang, Tiezhu Li, Yongjun Wang
Summary: The study found that treatment with beta-sitosterol cannot induce GR-mediated transactivation. Beta-sitosterol has the potential to inhibit the expression of GR target transrepressed genes without activating transactivation dependent genes. Molecular docking revealed that beta-sitosterol can bind to the ligand binding domain of GR but does not activate GR.
PROTEIN AND PEPTIDE LETTERS
(2021)
Article
Gastroenterology & Hepatology
Gangarao Davuluri, Nicole Welch, Jinendiran Sekar, Mahesha Gangadhariah, Khaled Alsabbagh Alchirazi, Maradumane L. Mohan, Avinash Kumar, Sashi Kant, Samjhana Thapaliya, McKenzie Stine, Megan R. McMullen, Rebecca L. McCullough, George R. Stark, Laura E. Nagy, Sathyamangla V. Naga Prasad, Srinivasan Dasarathy
Summary: Despite the high clinical significance of sarcopenia in alcohol-associated cirrhosis, the mechanisms of ethanol-induced impaired protein homeostasis are poorly understood. This study identified PP2A as a key mediator in ethanol-induced dysregulation of protein balance, suggesting potential therapeutic approaches targeting the PI3K gamma-PP2A axis.
Article
Biochemistry & Molecular Biology
Junjun Wei, Wei Sun, Xinhang Zheng, Shanshan Qiu, Shuangyu Jiao, Kevin Babilonia, Hisashi Koiwa, Ping He, Libo Shan, Wenxian Sun, Fuhao Cui
Summary: CPL1 functions as a negative regulator in plant defense, suppressing MAMP-induced MAPK cascade activation and immunity against bacterial pathogens.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Binna Lv, Lele Fan, Shidong Li, Manhong Sun
Summary: This study investigated the role of the mitogen-activated protein kinase (MAPK)-encoding gene Crmapk in mycoparasitism of Clonostachys chloroleuca 67-1. A high-quality yeast two hybrid (Y2H) library was constructed and proteins interacting with Crmapk were identified. Bioinformatics and transcriptome analysis revealed differentially expressed genes involved in the Fus3/Kss1-MAPK pathway.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jeong Hyeon Lee, Myoung Eun Choi, Hongchan An, Ju Won Moon, Hye Jin Yeo, Youngsup Song, Sung Eun Chang
Summary: BCI-215 effectively inhibits melanogenesis by suppressing the expression of microphthalmia-associated transcription factor (MITF) and its downstream enzymes. This study provides a new therapeutic strategy for treating pigmentation disorders using DUSP-related signaling modulators.
Article
Nutrition & Dietetics
Jacob Sellers, Abigail Brooks, Savanie Fernando, Gabrielle Westenberger, Sadie Junkins, Shauri Smith, Kisuk Min, Ahmed Lawan
Summary: The liver plays a crucial role in regulating glucose and lipid homeostasis in response to nutritional signals. Activation of MKP-1 in overnutrition helps control lipid metabolism in the liver, while the impact of MKP-1 in response to fasting is still unclear. Fasting induces upregulation of MKP-1, leading to resistance to hepatic steatosis and changes in key transcription factors involved in fatty acid and cholesterol metabolism, suggesting a beneficial role of MKP-1 in hepatic lipid utilization during fasting.
Article
Clinical Neurology
Anlong Jiang, Chanjuan Zhou, James Samsom, Shuxin Yan, Dian Zheng Yu, Zheng-ping Jia, Albert H. C. Wong, Fang Liu
Summary: A study found that disrupting the GR-FKBP51 complex can block the storage or retrieval of fear conditioned memories, providing a novel approach to alleviate fear associated memory in PTSD. However, this disruption does not impair other types of memory, suggesting that the GR-FKBP51 interaction could be a promising target for treating psychiatric disorders.
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Chuwei Lin, Aneirin Alan Lott, Wei Zhu, Craig P. Dufresne, Sixue Chen
Summary: This study used metabolomics and proteomics to investigate the metabolites and protein profiles of the mpk4 mutant and the wild type plants. It revealed the involvement of MPK4 in polyamine synthesis and photosynthesis, providing insights into the metabolic networks potentially regulated by MPK4.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Fouad S. El-mayet, Clinton Jones
Summary: Bovine alphaherpesvirus 1 (BoHV-1) infections in cows cause respiratory tract disorders and weaken immune responses, leading to bacterial pneumonia. After the initial infection, the virus enters a latent state in sensory neurons and cells in the pharyngeal tonsil. Stress can reactivate the virus from latency, and the cellular transcription factor Sp1 plays a role in enhancing viral replication and reactivation. The antibiotic mithramycin A has the potential to inhibit Sp1 function and reduce viral replication. The interaction between the glucocorticoid receptor and Sp1 stimulates viral gene expression and replication in response to stress.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Chari M. Noddings, Ray Yu-Ruei Wang, Jill L. Johnson, David A. Agard
Summary: The study reveals the cryo-electron microscopy structure of human GR-maturation complex (GR-Hsp90-p23), showing that the GR ligand-binding domain is restored to folded, ligand-bound conformation and threaded through the Hsp90 lumen, with p23 directly stabilizing native GR through a C-terminal helix, enhancing ligand binding. This client bound to Hsp90 structure contrasts with the unfolded kinase-Hsp90 structure, indicating that Hsp90 can dictate client-specific folding outcomes through direct co-chaperone-client interactions.
Article
Biochemistry & Molecular Biology
Hitesh Soni, Ravi Kumar, Praghalathan Kanthakumar, Adebowale Adebiyi
Summary: IL-1 beta induces Ca2+ entry via TRPA1 channels, leading to MAPK-dependent mesangial cell proliferation without stimulating fibronectin production.
Article
Biochemistry & Molecular Biology
Hong Seok Kim, Yun Hee Kang, Jisu Lee, Seung Ro Han, Da Bin Kim, Haeun Ko, Seyoun Park, Myung-Shin Lee
Summary: Hypoxia, a characteristic of the tumor microenvironment, induces the activation of HIF-1 alpha through factors like MAPK. MKP-3, a phosphatase specific to Erk1/2, plays a role in regulating HIF-1 alpha levels in hypoxic colon cancer cells, potentially serving as a therapeutic target for colon cancer treatment.
MOLECULES AND CELLS
(2021)
Article
Biochemistry & Molecular Biology
Qianqian Xu, Peiyu Zhang, Xiaoyan Han, Huwei Ren, Weiyue Yu, Wei Hao, Bowen Luo, Muhammad Imran Khan, Ni Chen
Summary: This study investigates the pivotal role of Nuclear factor-erythroid 2-related factor 2 (NRF2) in the activation of DNA damage repair in lung cancer cells after x-rays exposure. The study shows that NRF2 knockdown disrupts damaged DNA repair and homologous recombination, and NRF2 activation mediates DNA damage response via the MAPK pathway. These findings suggest that NRF2 plays a critical role in the development of radioresistance, which is of great significance for the development of radiotherapies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Agnes Jakab, Tamas Emri, Kinga Csillag, Anita Szabo, Fruzsina Nagy, Edina Baranyai, Zsofi Sajtos, Dora Geczi, Karoly Antal, Renato Kovacs, Krisztina Szabo, Viktor Dombradi, Istvan Pocsi
Summary: The study showed that the combination of oxidative stress treatment induced by menadione sodium bisulfite (MSB) and deletion of the CaPPZ1 gene can increase redox imbalance and reduce growth in Candida albicans cultures pre-treated with the glucocorticoid betamethasone (BM). The presence of BM significantly increased the number of differentially expressed genes in the MSB treated KO cultures, suggesting a synergistic effect between MSB treatment and CaPpz1 inhibition in developing a novel combinatorial antifungal strategy for steroid therapy.