期刊
MOLECULAR ECOLOGY RESOURCES
卷 11, 期 5, 页码 872-876出版社
WILEY
DOI: 10.1111/j.1755-0998.2011.03006.x
关键词
Agrostis stolonifera L.; creeping bentgrass; simple sequence repeats; SSRs
资金
- United States Golf Association
- Rutgers Center for Turfgrass Science
- New Jersey Agric. Exp. Stn.
Creeping bentgrass (Agrostis stolonifera L.) is a versatile, cross-pollinated, temperate and perennial turfgrass species. It occurs naturally in a wide variety of habitats and is also cultivated on golf courses, bowling greens and tennis courts worldwide. Isozymes and amplified fragment length polymorphisms (AFLPs) have been used to determine genetic diversity, and restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNA (RAPDs) were used to construct a genetic linkage map of this species. In the current report, we developed and characterized 215 unique genomic simple sequence repeat (SSR) markers in creeping bentgrass. The SSRs reported here are the first available markers in creeping bentgrass to date. Eight hundred and eighteen alleles were amplified by 215 SSR loci, an average of 3.72 alleles per locus. Fifty-nine per cent of those alleles segregated in a 1: 1 Mendelian fashion (P > 0.05). Twenty-two per cent had a distorted segregation ratio (P <= 0.05). These SSR markers will be useful for assessing genetic diversity in creeping bentgrass and will be important for the development of genetic linkage maps and identifying quantitative trait loci. These markers could enhance breeding programmes by improving the efficiency of selection techniques.
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