期刊
MOLECULAR ECOLOGY RESOURCES
卷 10, 期 1, 页码 193-196出版社
WILEY
DOI: 10.1111/j.1755-0998.2009.02726.x
关键词
genotyping; high-resolution melting analysis; nuclear DNA; population studies; SNP; swordfish
资金
- McDaniel Charitable Foundation
- Texas Institute of Oceanography (TIO)
High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.
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