期刊
MOLECULAR ECOLOGY RESOURCES
卷 9, 期 1, 页码 405-408出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1755-0998.2008.02254.x
关键词
cross-species amplification; delta smelt; Hypomesus transpacificus; microsatellites; Osmeridae; primers
资金
- CESU [813327J011]
We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy-Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).
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