期刊
MOLECULAR ECOLOGY RESOURCES
卷 9, 期 1, 页码 354-356出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1755-0998.2008.02443.x
关键词
Chelonia mydas; green turtle; microsatellites; primers; sea turtles; tetranucleotide repeats; trinucleotide repeats
资金
- NOAA Fisheries' Southwest Fisheries Science Center
We describe primers and polymerase chain reaction conditions to amplify 12 microsatellite loci from the green turtle (Chelonia mydas), including one dinucleotide, four trinucleotide and seven tetranucleotide loci. The primers were tested on 78 individuals from a Pacific population nesting in the Hawaiian Islands. The primer pairs developed in this study yielded an average of 8.33 alleles per locus (range of 3-15 alleles), an average observed heterozygosity of 0.668 (range 0.309-0.910), and an average polymorphic information content of 0.647 (range 0.287-0.894).
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