期刊
MOLECULAR ECOLOGY RESOURCES
卷 9, 期 3, 页码 853-855出版社
WILEY
DOI: 10.1111/j.1755-0998.2009.02525.x
关键词
Crassostrea gigas; EST-SSR markers; null alleles; Pacific oyster
资金
- EU projects Marine Genomics
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
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