Article
Biochemistry & Molecular Biology
Kang Hee Kho, Zahid Parvez Sukhan, Shaharior Hossen, Yusin Cho, Won-Kyo Lee, Ill-Sup Nou
Summary: This study analyzed the expression of QTLs for growth-related traits in Pacific abalone at different ages. It was found that different QTLs influence growth-related traits at different ages, but there are also age-specific multiple-trait QTLs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Junyu Liu, Wenzhu Peng, Feng Yu, Weihong Lin, Yawei Shen, Wenchao Yu, Shihai Gong, Huiyang Huang, Weiwei You, Xuan Luo, Caihuan Ke
Summary: This study developed a novel multiple single-nucleotide polymorphism (mSNP) liquid array, named Baoxin-I, for genome-wide evaluation of the economically important Pacific abalone. The performance evaluation of Baoxin-I showed high efficiency and accuracy in population separation testing and genomic selection.
Article
Multidisciplinary Sciences
Sungwoo Yoon, Mi Ae Kim, Jung Sick Lee, Young Chang Sohn
Summary: This study identified the LFRFa and short neuropeptide F receptor in Pacific abalone, demonstrating their key roles in food intake and reproduction. The expression levels of LFRFa are significantly higher in mature female abalone, and injection of LFRFa induces egg release and spawning behavior while suppressing food intake.
Article
Biochemistry & Molecular Biology
Md. Rajib Sharker, Soo Cheol Kim, Shaharior Hossen, Kanij Rukshana Sumi, Sang Ki Choi, Kap Seong Choi, Kang Hee Kho
Summary: This study isolated and characterized a CA I from the mantle tissue of Pacific abalone, Haliotis discus hannai. The CA I protein showed higher expression levels in the mantle and strong signals in the tissue responsible for nacreous layer formation, indicating its potential role in shell biomineralization of abalone.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Fisheries
Wenzhu Peng, Feng Yu, Yiyu Wu, Yifang Zhang, Chengkuan Lu, Yi Wang, Zekun Huang, Yisha Lu, Nan Chen, Xuan Luo, Weiwei You, Caihuan Ke
Summary: This study conducted a genome-wide association study on growth traits of Pacific abalone, identifying 263 significant SNPs associated with growth traits and validating 7 reliable growth-associated markers. Additionally, 62 candidate genes were found to potentially affect growth-related traits.
Article
Genetics & Heredity
Jianfang Huang, Mingcan Zhou, Weiwei You, Xuan Luo, Caihuan Ke
Summary: This study aimed to better understand the characterization and biological function of BMP7 of Haliotis discus hannai (hdh-BMP7) via cloning and sequencing analysis. The results showed that hdh-BMP7 mRNA was widely expressed in all the examined tissues of H. discus hannai and was associated with growth traits. Silencing hdh-BMP7 led to decreased expression levels of hdh-BMPR I, hdh-BMPR II, hdh-smad1, and hdh-MHC, as well as reduced shell length, shell width, and total weight in H. discus hannai. The results suggest that BMP7 gene plays a positive role in the growth of H. discus hannai.
Article
Virology
Tomomasa Matsuyama, Ikunari Kiryu, Mari Inada, Tomokazu Takano, Yuta Matsuura, Takashi Kamaishi
Summary: The study revealed that H. gigantea, H. discus discus, and H. discus hannai are susceptible to Abalone asfa-like virus (AbALV), while H. diversicolor is not. Through experimental infection and immunohistological observations, it confirmed that AbALV is the etiological agent of abalone amyotrophia.
Article
Biochemistry & Molecular Biology
Shaharior Hossen, Md. Rajib Sharker, Yusin Cho, Zahid Parvez Sukhan, Kang Hee Kho
Summary: The study aimed to improve the sperm cryopreservation technique for Pacific abalone using AFPIII, which significantly enhanced post-thaw sperm quality parameters. Combining AFPIII with DMSO resulted in improved motility, acrosome integrity, and plasma membrane integrity of the sperm after cryopreservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Yiyu Wu, Wenzhu Peng, Yi Wang, Zekun Huang, Yu Feng, Zhaofang Han, Xuan Luo, Weiwei You, Caihuan Ke
Summary: This study identified the sex-determination region and developed sex-specific markers in the Pacific abalone. These markers can be utilized for sex discrimination in breeding projects. Additionally, candidate genes involved in the sex determination were identified through haplotype analysis and quantitative gene expression.
Article
Fisheries
Wenchao Yu, Junyu Liu, Feng Yu, Yawei Shen, Shihai Gong, Yisha Lu, Wenzhu Peng, Yi Wang, Yang Gan, Qizhen Xiao, Xuan Luo, Weiwei You, Caihuan Ke
Summary: This study measured the growth and feed efficiency traits of Pacific abalone and established an individual-based accurate measurement method. The results showed that residual feed intake (RFI) can be used as a suitable trait for indicating the feed efficiency of abalone, and it is genetically correlated with other traits.
Article
Biochemistry & Molecular Biology
Yanglei Jia, Fei Xu, Xiao Liu
Summary: Aquaporins (AQPs) are a group of proteins that mediate specific permeation of water and small solutes, and play important roles in osmoregulation and nutrition in aquatic animals. Genome-wide characterization of AQPs in Pacific abalone showed that tandem duplication and retroduplication led to the expansion and diversification of AQP genes. Structural analysis revealed abnormal characteristics in tandem duplicated AQPs, which altered pore diameter and properties of the inner surface, allowing the passage of molecules other than water. Functional analysis indicated that the abnormal region of tandem duplicated AQPs led to different permeability, suggesting neofunctionalization. Mutation analysis further supported the mechanism of neofunctionalization. These findings provide strong evidence for the structural and functional diversity of AQPs in Pacific abalone and shed light on the evolution of AQPs in molluscs.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Environmental Sciences
Jianpeng Zhang, Yi Wang, Zekun Huang, Mingyi Cai, Weiwei You, Ying Lu, Xuan Luo, Caihuan Ke
Summary: In this study, chromosome-specific probes were developed for Pacific abalone and used to study chromosome evolution in related species. The probes were successfully anchored onto pseudochromosomes of the Pacific abalone genome and validated for their specificity. The study also revealed a chromosome rearrangement event between Pacific abalone and Xishi abalone. This research provides important tools for cytogenetics and genomics research in the family Haliotidae.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Hanjiao Luo, Junzhu Xiao, Yonghua Jiang, Yizhou Ke, Caihuan Ke, Mingyi Cai
Summary: This study identified sex-linked genomic segments and markers in Pacific abalone through genome resequencing, providing valuable information for further research on the molecular mechanism of sex determination and sex-control breeding in this species.
Article
Fisheries
Mi-Jin Choi, Young Dae Oh, Yeo Reum Kim, Han Kyu Lim, Jong-Myoung Kim
Summary: Analysis of intestinal microbial communities in Pacific abalones revealed higher microbial species diversity in small abalones with slower growth rates, with certain microbial genera more prevalent in large abalones.
Article
Biology
Jianfang Huang, Mingcan Zhou, Jianming Chen, Caihuan Ke
Summary: The Haliotis discus hannai is an economically important species in China, but slow growth and small size have caused losses to farmers. Molecular genetic manipulation is the most effective way to breed high-quality abalone with rapid growth rates. This study revealed that the myostatin gene played a critical role in the growth regulation of H. discus hannai and provided a reference for mollusk breeding using molecular markers for growth traits.
