期刊
MOLECULAR ECOLOGY RESOURCES
卷 8, 期 5, 页码 1062-1064出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1755-0998.2008.02155.x
关键词
Aplexa marmorata; Gastropoda; microsatellites; Mollusca; snail
资金
- JCJC program of the French Agence Nationale de la Recherche
- cosystemes Tropicaux [00356]
- Ministere de l'Ecologie et do Developpement Durable
We describe 15 new variable microsatellites in the freshwater snail Aplexa (Physa) marmorata, as well as conditions for multiplexing and simultaneous genotyping sets of loci. Two to 11 alleles were detected per locus over the five populations studied, and up to eight alleles per locus and population. The observed heterozygosity was extremely low, most probably as the consequence of high selfing rates. The genetic differentiation among populations was large. These markers will constitute efficient tools for investigating the population structure in fragmented habitats. Cross-species amplification in three Physa species (P acuta, P. fontinalis and P. gyrina) was rather unsuccessful.
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