期刊
MOLECULAR CELL
卷 53, 期 5, 页码 843-853出版社
CELL PRESS
DOI: 10.1016/j.molcel.2014.01.031
关键词
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资金
- Netherlands Organization for Scientific Research
- European Research Council
- Cancer Genomics Center.nl
- Swedish Research Council
- Swedish Foundation for Strategic Research
- Swedish Cancer Society
- Swedish Childhood Cancer Foundation
During the cell cycle, DNA duplication in S phase must occur before a cell divides in mitosis. In the intervening G2 phase, mitotic inducers accumulate, which eventually leads to a switch-like rise in mitotic kinase activity that triggers mitotic entry. However, when and how activation of the signaling network that promotes the transition to mitosis occurs remains unclear. We have developed a system to reduce cell-cell variation and increase accuracy of fluorescence quantification in single cells. This allows us to use immunofluorescence of endogenous marker proteins to assess kinetics from fixed cells. We find that mitotic phosphorylations initially occur at the completion of S phase, showing that activation of the mitotic entry network does not depend on protein accumulation through G2. Our data show insights into how mitotic entry is linked to the completion of S phase and forms a quantitative resource for mathematical models of the human cell cycle.
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