Article
Multidisciplinary Sciences
Ann-Marie K. Shorrocks, Samuel E. Jones, Kaima Tsukada, Carl A. Morrow, Zoulikha Belblidia, Johanna Shen, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Andrew N. Blackford
Summary: The study reveals that the BTR complex contains multiple conserved motifs that interact cooperatively with the single-stranded DNA-binding protein RPA, facilitating BLM recruitment to stalled replication forks for promoting their restart after replication stress.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Deepak Bhat, Samuel Hauf, Charles Plessy, Yohei Yokobayashi, Simone Pigolotti, Armita Nourmohammad
Summary: In this study, a new method was proposed to infer the dynamics of Replisomes from DNA abundance distribution. The method was applied to measure the DNA abundance distribution in Escherichia coli populations growing at different temperatures. The results showed that the speed of Replisomes increases with temperature and exhibits wave-like variations along the genome, which correlates with the variation of mutation rate. This study provides insights into the replication dynamics of bacteria.
Review
Microbiology
Benoit Arcangioli, Serge Gangloff
Summary: This article describes how Schizosaccharomyces pombe, a variant of the budding yeast, relies on efficient genetic sex determination and alternating haploid/diploid phases to achieve high reproductive efficiency in response to environmental conditions. By exploring the mating-type switching process and the interaction between genetics and epigenetics, it underscores the significance of basic research in gaining a better understanding of chromatin biology.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Michael A. Boemo
Summary: This paper introduces a new software, DNAscent v2, for high-throughput, single-molecule detection of DNA replication dynamics with fast and accurate detection of the thymidine analogue BrdU. The software excels in accuracy, speed, and versatility across different experimental protocols.
Article
Multidisciplinary Sciences
Karel Naiman, Eduard Campillo-Funollet, Adam T. Watson, Alice Budden, Izumi Miyabe, Antony M. Carr
Summary: Replication forks restarted by homologous recombination utilize Pol delta to synthesize both strands without maturing to a delta/epsilon configuration, while the template for the lagging strand remains as a gap filled in by Pol delta later. These restarted forks are able to progress through barriers that arrest canonical forks, demonstrating stability of the 3' single strand during increased resection.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
P. Logan Schuck, Lauren E. Ball, Jason A. Stewart
Summary: The research suggests that the ssDNA-binding protein CST helps in the maintenance of sister chromatid cohesion (SCC), particularly under replication stress conditions. CST associates with the cohesin complex and is involved in maintaining or remodeling SCC after DNA replication fork stalling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Ilona Faustova, Mart Loog
Summary: Study by Hossain et al. (2021) reveals that ORC1 and CDC6 interact during pre-replicative complex formation in G1, mediated by SLiMs in IDRs and regulated by CDKs.
Article
Cell Biology
Andrew Dolson, Safia Mahabub Sauty, Kholoud Shaban, Krassimir Yankulov
Summary: Dbf4-Dependent Kinase (DDK) plays an essential role in DNA replication and repair, although some of its activities are not essential for viability. Recent studies have shown that DDK also plays a key role in regulating genome-wide origin firing and replication-coupled chromatin assembly.
Article
Biochemistry & Molecular Biology
Chia-Lun Guh, Kai-Hang Lei, Yi-An Chen, Yi-Zhen Jiang, Hao-Yen Chang, Hungjiun Liaw, Hung-Wen Li, Hsin-Yung Yen, Peter Chi
Summary: This study provides evidence for an unknown function of RAD51 paralogs in synergizing with RAD51 nucleoprotein filament to prevent degradation of stressed replication forks.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Anjali Mann, Miguel Angel Ramirez-Otero, Anna De Antoni, Yodhara Wijesekara Hanthi, Vincenzo Sannino, Giorgio Baldi, Lucia Falbo, Anna Schrempf, Sara Bernardo, Joanna Loizou, Vincenzo Costanzo
Summary: POLO plays an important role in repairing DNA double-strand breaks in HR-defective tumors. It is found that POLO processes stalled Okazaki fragments, preventing the accumulation of ssDNA gaps on lagging strands in the absence of RAD51. Inhibition of POLO's DNA polymerase activity leads to unprotected fork gaps, which are cleaved by the MRE11-NBS1-CtIP endonuclease, causing asymmetric single-ended DSBs that impede the survival of BRCA2-defective cells.
Article
Multidisciplinary Sciences
Mihyun Kim, Hyun-Suk Kim, Areetha D'Souza, Kaitlyn Gallagher, Eunwoo Jeong, Kateryna Ogorodnik Le Meur, Chi-Lin Tsai, Miaw-Sheue Tsai, Minyong Kee, John A. Tainer, Jung-Eun Yeo, Walter J. Chazin, Orlando D. Scharer
Summary: The XPA and RPA proteins play essential roles in the assembly of the preincision complex in the nucleotide excision repair pathway. Mutations in the interaction sites of XPA and RPA inhibit the physical interaction and reduce the NER activity. The interaction between XPA-N and RPA32C is important for the initial association of XPA with NER complexes, while the interaction between XPA DBD and RPA70AB is needed for structural organization of the complex to license the dual incision reaction. The shape of the NER bubble is not colinear as previously thought, but rather the unwound DNA assumes a U-shape with the junctions localized in close proximity. The interaction between XPA and RPA70 is key for the organization of the NER preincision complex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Kumar Somyajit, Julian Spies, Fabian Coscia, Ufuk Kirik, Maj-Britt Rask, Ji-Hoon Lee, Kai John Neelsen, Andreas Mund, Lars Juhl Jensen, Tanya T. Paull, Matthias Mann, Jiri Lukas
Summary: The inhibition of ribonucleotide reductase leads to metabolic imbalance and accumulation of reactive oxygen species in cell nuclei, triggering a cascade of reactions involving ATM kinase and MRE11 nuclease. This process affects DNA replication and repair, impacting cancer evolution and tumor therapy.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Sai Li, Michael R. Wasserman, Olga Yurieva, Lu Bai, Michael E. O'Donnell, Shixin Liu
Summary: This study found that the ORC can eject histones from a nucleosome in an ATP-dependent manner. It selectively evicts H2A-H2B dimers but leaves the (H3-H4)2 tetramer on DNA. It also discriminates canonical H2A from the H2A.Z variant, evicting the former while retaining the latter. These findings suggest that the ORC is a bona fide nucleosome remodeler that functions to create a local chromatin environment optimal for origin activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Martin Meagher, Alexander Myasnikov, Eric J. Enemark
Summary: The ATPase ring of the replication forks is essential for DNA replication and disruption of this ring can lead to genetic instability and diseases such as cancer. Using cryo-EM, researchers have found that the ATPase ring of the archaeal organism Saccharolobus solfataricus binds to the DNA in two different modes, revealing a mechanism for the alternating steps of DNA dissociation and re-association during DNA translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Evgeniya N. Andreyeva, Alexander Emelyanov, Markus Nevil, Lu Sun, Elena Vershilova, Christina A. Hill, Michael-C Keogh, Robert J. Duronio, Arthur Skoultchi, Dmitry Fyodorov
Summary: Asynchronous replication of chromosome domains during S phase is essential for genome function, but the mechanisms establishing early versus late replication domains in different cell types remain incompletely understood. A study in Drosophila found that the complex SUMM4, composed of SUUR and Mod(Mdg4)-67.2, can delay the replication of intercalary heterochromatin by attenuating the progression of replication forks, thereby implicating a chromatin barrier in replication control.
