Article
Agriculture, Multidisciplinary
Yang Sheng-di, Guo Da-long, Pei Mao-song, Wei Tong-lu, Liu Hai-nan, Bian Lu, Yu Ke-ke, Zhang Guo-hai, Yu Yi-he
Summary: This study provides new insights into the structure, evolution, and participation of DEAD-box RNA helicase genes in the response to drought stress in grapevines.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Review
Cell Biology
Shringar Rao, Tokameh Mahmoudi
Summary: This review provides an overview of the functions and mechanisms of DEAD-box family proteins in HIV-1 vRNA metabolism. It discusses the implications of these interactions in viral infection, viral latency, and cell death, and speculates on the potential of DEAD-box protein inhibitors as therapeutics for controlling the HIV-1 pandemic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mst. Sufara Akhter Banu, Kazi Md. Kamrul Huda, Md. Harun-Ur-Rashid, Shahanaz Parveen, S. M. Shahinul Islam, Narendra Tuteja
Summary: In this study, a DEAD-box RNA helicase OsDB10 was cloned and functionally characterized. The results showed that OsDB10 expression was upregulated in response to salt, heat, cold, and ABA treatment, and transgenic rice plants overexpressing OsDB10 exhibited improved tolerance to salinity stress. This enhanced tolerance was attributed to improved photosynthesis, ion homeostasis, reactive oxygen species (ROS) scavenging, and transcriptional regulation.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Chi Xu, Zhanhui Zhang, Juan He, Yongsheng Bai, Jie Cui, Lin Liu, Jihua Tang, Guiliang Tang, Xuemei Chen, Beixin Mo
Summary: RCF1 is shown to play a role in pri-miRNA processing and splicing, as well as pre-mRNA splicing in Arabidopsis thaliana. A mutant with miRNA biogenesis defects was identified, and it was found to be caused by a recessive point mutation in RCF1 (rcf1-4). RCF1 promotes D-body formation and facilitates the interaction between pri-miRNAs and HYL1, and rcf1-4 exhibits a global splicing defect in intron-containing pri-miRNAs and pre-mRNAs. This study uncovers the importance of RCF1 in miRNA biogenesis and RNA splicing in Arabidopsis.
Review
Genetics & Heredity
Michael Cargill, Rasika Venkataraman, Stanley Lee
Summary: DEAD-box RNA helicases are crucial regulators of RNA metabolism and are linked to genome stability and cancer development. They play important roles by regulating the expression of factors related to genome stability and repairing DNA damage.
Article
Biochemistry & Molecular Biology
Boris Bonaventure, Antoine Rebendenne, Ana Luiza Chaves Valadao, Mary Arnaud-Arnould, Segolene Gracias, Francisco Garcia de Gracia, Joe McKellar, Emmanuel Labaronne, Marine Tauziet, Valerie Vivet-Boudou, Eric Bernard, Laurence Briant, Nathalie Gros, Wassila Djilli, Valerie Courgnaud, Hugues Parrinello, Stephanie Rialle, Mickael Blaise, Laurent Lacroix, Marc Lavigne, Jean-Christophe Paillart, Emiliano P. Ricci, Reiner Schulz, Nolwenn Jouvenet, Olivier Moncorge, Caroline Goujon
Summary: DDX42 has been identified as an intrinsic antiviral inhibitor, restricting HIV-1 and other RNA viruses. It does not impact the replication of several negative-strand RNA viruses, suggesting a specific mode of action on target cells.
Article
Multidisciplinary Sciences
Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai
Summary: RNA helicases, such as Prp16 and Prp22, promote correct mRNA synthesis by binding and proofreading on their RNA substrates in defined spliceosome complexes. The stability of spliceosome during exon ligation may affect the proofreading activity of helicases. The new method psiCLIP provides key insights into the binding and proofreading activity of spliceosomal RNA helicases.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Olivier Finet, Carlo Yague-Sanz, Lara Katharina Kruger, Phong Tran, Valerie Migeot, Max Louski, Alicia Nevers, Mathieu Rougemaille, Jingjing Sun, Felix G. M. Ernst, Ludivine Wacheul, Maxime Wery, Antonin Morillon, Peter Dedon, Denis L. J. Lafontaine, Damien Hermand
Summary: This study reveals the importance of RNA modifications in gene expression control, and develops a new integrated pipeline to detect modifications in a transcriptome-wide manner. The researchers identify a novel RNA modification and demonstrate its biological function in mRNA of fission yeast. They also find that the modification is evolutionarily conserved in human cells.
Article
Biochemistry & Molecular Biology
Tomoyuki Takase, Motoki Shimizu, Shigekazu Takahashi, Keiichirou Nemoto, Fumina Goto, Chiharu Yoshida, Akira Abe, Masahiro Nishihara
Summary: This study investigated the molecular mechanisms underlying the control of flowering time in Japanese cultivated gentians. Transcriptome profiling and analysis of transcription factor genes revealed their coexpression with the flowering time-related gene GtFT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Uciel Chorostecki, Ester Saus, Toni Gabaldon
Summary: Long non-coding RNAs (lncRNAs) play key cellular functions through interactions with proteins and other molecules. NORAD, an abundant and highly conserved lncRNA, regulates genomic stability by interacting with proteins and microRNAs. Experimental determination of NORAD's secondary structure reveals structural stability in spacer regions and core conserved motifs across repeat units.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jun-Xiu Wang, En-Bo Ma, Jian-Zhen Zhang, Shu-Ping Xing
Summary: Cellular and molecular mechanisms play a key role in maintaining midgut homeostasis, with LmDDX47 acting as an important regulator by controlling 18S rRNA synthesis and protein modification levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Paul T. Winnard, Farhad Vesuna, Venu Raman
Summary: DDX3X, a multifunctional protein utilized by various viruses, plays a crucial role in viral metabolism. Modulating the functions of DDX3 during viral infections could effectively suppress viral production and have a significant impact on safely and efficiently subduing a broad spectrum of viral infections.
ANTIVIRAL RESEARCH
(2021)
Article
Microbiology
Hugo L. de Araujo, Beatriz A. Picinato, Alan P. R. Lorenzetti, Nisansala S. Muthunayake, I. W. Rathnayaka-Mudiyanselage, Naara M. dos Santos, Jared Schrader, Tie Koide, Marilis V. Marques
Summary: The absence of the RNA helicase RhlB in Caulobacter crescentus causes important changes in gene expression and cell physiology. These changes are likely due, at least in part, to inefficient RNA processing by the RNA degradosome, especially at low-temperature conditions.
MICROBIOLOGY SPECTRUM
(2023)
Review
Cell Biology
Lu Zhang, Xiaogang Li
Summary: RNA helicases play essential roles in regulating the cell cycle by influencing various cellular processes such as cell proliferation, cell cycle arrest, and apoptosis, impacting multiple diseases. Studies have shown their involvement in regulating progression through different phases of the cell cycle.
Article
Biochemical Research Methods
Ravi Shankar Singh, Ananna Bhadra Arna, He Dong, Manisha Yadav, Aanchal Aggarwal, Yuliang Wu
Summary: DDX43, a member of the DEAD-box protein family, exhibits ATP-dependent RNA and DNA helicase activity. Its KH domain selectively binds pyrimidine-rich ssDNA and ssRNA. DDX43 is overexpressed in many tumors and thus holds potential as a target for cancer therapy.
Article
Biochemical Research Methods
Amy L. Cherry, Csaba Finta, Mikael Karlstrom, Qianren Jin, Thomas Schwend, Juan Astorga-Wells, Roman A. Zubarev, Mark Del Campo, Angela R. Criswell, Daniele de Sanctis, Luca Jovine, Rune Toftgard
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2013)
Article
Biochemical Research Methods
Mark Del Campo, Alan M. Lambowitz
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2009)
Article
Biochemistry & Molecular Biology
Georg Mohr, Mark Del Campo, Sabine Mohr, Quansheng Yang, Huijue Jia, Eckhard Jankowsky, Alan M. Lambowitz
JOURNAL OF MOLECULAR BIOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Mark Del Campo, Sabine Mohr, Yue Jiang, Huijue Jia, Eckhard Jankowsky, Alan M. Lambowitz
JOURNAL OF MOLECULAR BIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Georg Mohr, Mark Del Campo, Kathryn G. Turner, Benjamin Gilman, Rachel Z. Wolf, Alan M. Lambowitz
JOURNAL OF MOLECULAR BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Jeffrey P. Potratz, Mark Del Campo, Rachel Z. Wolf, Alan M. Lambowitz, Rick Russell
JOURNAL OF MOLECULAR BIOLOGY
(2011)
Article
Multidisciplinary Sciences
Anna L. Mallam, Mark Del Campo, Benjamin Gilman, David J. Sidote, Alan M. Lambowitz
Article
Multidisciplinary Sciences
Yingfeng Chen, Jeffrey P. Potratz, Pilar Tijerina, Mark Del Campo, Alan M. Lambowitz, Rick Russell
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2008)
Article
Multidisciplinary Sciences
Anna L. Mallam, Inga Jarmoskaite, Pilar Tijerina, Mark Del Campo, Soenke Seifert, Liang Guo, Rick Russell, Alan M. Lambowitz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Multidisciplinary Sciences
Jonathan S. Edwards, Laurie Betts, Monica L. Frazier, Rebecca M. Pollet, Stephen M. Kwong, William G. Walton, W. Keith Ballentine, Julianne J. Huang, Sohrab Habibi, Mark Del Campo, Jordan L. Meier, Peter. B. Dervan, Neville Firth, Matthew R. Redinbo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
Dmitriy A. Markov, Maria Savkina, Michael Anikin, Mark Del Campo, Karen Ecker, Alan M. Lambowitz, Jon P. De Gnore, William T. McAllister
Article
Chemistry, Inorganic & Nuclear
Prabhakaran Rajasekar, Swechchha Pandey, Joseph D. Ferrara, Mark Del Campo, Pierre Le Magueres, Ramamoorthy Boomishankar
INORGANIC CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Khai-Nghi Truong, Sho Ito, Jakub M. Wojciechowski, Christian R. Goeb, Christian J. Schuermann, Akihito Yamano, Mark Del Campo, Eiji Okunishi, Yoshitaka Aoyama, Tomohiro Mihira, Naoki Hosogi, Jordi Benet-Buchholz, Eduardo Carmelo Escudero-Adan, Fraser J. White, Joseph D. Ferrara, Robert Buecker
Summary: This paper discusses best practices for structure determination using three-dimensional electron diffraction. By studying cryo-transfer of the sample, simplified and fast workflow for dynamical diffraction calculations, and optimal crystal-to-detector distance, the paper identifies key factors that are crucial for improving the accuracy of structure determination.
Article
Biochemistry & Molecular Biology
Quansheng Yang, Mark Del Campo, Alan M. Larnbowitz, Eckhard Jankowsky