Review
Biochemistry & Molecular Biology
Paola De Magistris
Summary: Nuclear export of mRNA through the NPC is crucial for protein translation in eukaryotic cells, with mRNAs forming ribonuclear particles with proteins for translocation. mRNPs undergo compositional and conformational changes during NPC transport, with multiple quality control steps to ensure proper export of mature transcripts to the cytoplasm. Opportunities for mechanistic understanding of nuclear export are mentioned in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhiling Kuang, Jiyuan Ke, Jiong Hong, Zhongliang Zhu, Liwen Niu
Summary: PCI domain proteins play important roles in post-transcriptional gene regulation. In this study, the structure of yeast Thp3-Csn12-Sem1 ternary complex was determined, revealing significant structural differences compared to the Sac3-Thp1-Sem1 complex. This structure provides a foundation for further exploring its specific recruitment to the spliceosome.
NUCLEIC ACIDS RESEARCH
(2022)
Editorial Material
Cell Biology
Yoseop Yoon, Yongsheng Shi
Summary: In this article, two independent studies successfully reconstituted the processing of human pre-mRNA 3' ends using defined components in vitro. These studies are of great importance for understanding the nature and essential components of this biological process.
GENES & DEVELOPMENT
(2022)
Review
Genetics & Heredity
Lindsey V. Soles, Yongsheng Shi
Summary: APA is a dynamic and highly regulated process that produces multiple mRNA isoforms; mis-regulation of APA has been linked to a variety of diseases. Recent studies have revealed important roles for epigenetic mechanisms in APA regulation.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Ananthanarayanan Kumar, Conny W. H. Yu, Juan B. Rodriguez-Molina, Xiao-Han Li, Stefan M. V. Freund, Lori A. Passmore
Summary: The translation provided describes a detailed study of the essential subunit Fip1 in the CPF/CPSF multiprotein complex, revealing the importance of its dynamics in coordinating cleavage and polyadenylation processes at the 3' end.
GENES & DEVELOPMENT
(2021)
Article
Cell Biology
Ryuta Asada, Andrew Dominguez, Ben Montpetit
Summary: This study investigates the stoichiometry and co-occupancy of 15 RBPs within mRNPs from Saccharomyces cerevisiae using a single-molecule imaging assay. The results show compositional heterogeneity and plasticity in mRNPs under different growth conditions. The major co-occupants of mRNPs are Yra1, Nab2, and Npl3. Yra1-bound mRNPs with multiple copies specifically co-occur with the THO complex and are assembled on mRNAs biased by transcript length and RNA secondary structure. Depletion of Yra1 leads to decreased compaction of nuclear mRNPs, suggesting a packaging function.
Article
Cell Biology
Moritz Schmidt, Florian Kluge, Felix Sandmeir, Uwe Kuehn, Peter Schaefer, Christian Tueting, Christian Ihling, Elena Conti, Elmar Wahle
Summary: In this study, the endonucleolytic cleavage of eukaryotic mRNA 3' ends followed by polyadenylation was reconstituted using overproduced and purified proteins. A minimal list of 14 essential polypeptides and 2 stimulatory polypeptides for RNA processing were identified. Key findings include the stimulation of cleavage by cleavage factor I and the essential requirement of RBBP6 in contacting and activating the endonuclease CPSF73.
GENES & DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Mikhail Metelev, Erik Lundin, Ivan L. Volkov, Arvid H. Gynna, Johan Elf, Magnus Johansson
Summary: Metelev et al. used single-molecule tracking to study translation kinetics in E. coli cells and found that over 90% of ribosomes are engaged in translation at any given time. They also discovered that translation of poly-cistronic mRNAs is predominantly initiated by 70S re-initiation instead of 30S re-initiation. Additionally, they observed significant binding of ribosomes with altered anti-Shine-Dalgarno sequences to endogenous mRNAs.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Daniel Heller-Trulli, Huiyun Liu, Srimoyee Mukherjee, Claire L. L. Moore
Summary: This study reveals the important role of Ube3d in cellular differentiation and its effect on mRNA processing. Knockout of Ube3d inhibits the differentiation of 3T3-L1 cells into mature adipocytes and leads to global changes in mRNA levels. Overexpression of UBE3D or CPSF73 can rescue the differentiation defect and partially restore protein levels.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Fabian Poetz, Joshua Corbo, Yevgen Levdansky, Alexander Spiegelhalter, Doris Lindner, Vera Magg, Svetlana Lebedeva, Jorg Schweiggert, Johanna Schott, Eugene Valkov, Georg Stoecklin
Summary: The study identified RNF219 as an acetylation-regulated cofactor of the CCR4-NOT complex, which inhibits mRNA degradation mediated by the complex through interaction with NOT9. RNF219 attenuates global mRNA turnover in cells with a differential requirement of its RING domain.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jing Li, Luisa Querl, Ivo Coban, Gabriela Salinas, Heike Krebber
Summary: CF IB/Hrp1 is a component of the CPF-CF complex responsible for 3' cleavage and maturation of pre-mRNAs. Its main function in the complex is the nuclear mRNA quality control of proper 3' cleavage. Hrp1 acts as a retention factor that prevents transcripts from leaving the nucleus until processing is completed. Only after proper 3' cleavage, Hrp1 recruits the export receptor Mex67, allowing nuclear export.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Immunology
Aarthi Vijayakumar, Annsea Park, Joan A. Steitz
Summary: Eukaryotic mRNA 3'-end processing is a critical step in gene expression regulation, and disruption of this process by viral proteins can lead to host shutoff and impaired antiviral response. Viruses may utilize the effects of read-through transcripts to modulate host protein production and cellular machinery.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Yali Wang, Weimin Zhang, Wenzhong Liu, Lijie Huang, Yan Wang, Dan Li, Guangchao Wang, Zitong Zhao, Xinming Chi, Yu Xue, Yongmei Song, Xuefeng Liu, Qimin Zhan
Summary: This study revealed a novel lncRNA-guided mechanism of lymph node metastasis in ESCC, in which VESTAR played a key role in lymphatic metastasis. This may provide a potential target for the treatment of ESCC lymphatic metastasis.
Review
Biochemistry & Molecular Biology
Vytaute Boreikaite, Lori A. Passmore
Summary: The formation of the 3' end of a eukaryotic mRNA is a crucial step in mRNA maturation. Protein factors such as CPSF and CPF are responsible for cleaving the pre-mRNA, adding a poly(A) tail, and regulating transcription. The selection of the cleavage site and the length of the poly(A) tail play important roles in mRNA translation, stability, and localization.
ANNUAL REVIEW OF BIOCHEMISTRY
(2023)
Article
Virology
Zhimin Tang, Wangtai Luo, Zhihong Huang, Meijin Yuan, Wenbi Wu, Kai Yang
Summary: The study revealed that Spodoptera frugiperda mRNA export factor (SfMEF) interacts with Autographa californica multiple nucleopolyhedrovirus orf34 (ac34) and mediates its nuclear import. Mutations in a potential zinc finger motif of Ac34 disrupted the interaction between Ac34 and SfMEF, leading to decreased production of budded virions (BV). This interaction presents a new nucleocytoplasmic transport pathway utilized by alphabaculoviruses for successful viral replication within infected cell nuclei.
Article
Biochemistry & Molecular Biology
Becky Fusby, Soojin Kim, Benjamin Erickson, Hyunmin Kim, Martha L. Peterson, David L. Bentley
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Nova Fong, Kristopher Brannan, Benjamin Erickson, Hyunmin Kim, Michael A. Cortazar, Ryan M. Sheridan, Tram Nguyen, Shai Karp, David L. Bentley
Editorial Material
Biochemistry & Molecular Biology
David L. Bentley
Article
Biochemical Research Methods
Ryan M. Sheridan, David L. Bentley
Review
Biochemistry & Molecular Biology
Tassa Saldi, Michael A. Cortazar, Ryan M. Sheridan, David L. Bentley
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Nova Fong, Tassa Saldi, Ryan M. Sheridan, Michael A. Cortazar, David L. Bentley
Article
Cell Biology
Christopher C. Ebmeier, Benjamin Erickson, Benjamin L. Allen, Mary A. Allen, Hyunmin Kim, Nova Fong, Jeremy R. Jacobsen, Kaiwei Liang, Ali Shilatifard, Robin D. Dowell, William M. Old, David L. Bentley, Dylan J. Taatjes
Article
Cell Biology
Tassa Saldi, Nova Fong, David L. Bentley
GENES & DEVELOPMENT
(2018)
Article
Cell Biology
Nova Fong, Hyunmin Kim, Yu Zhou, Xiong Ji, Jinsong Qiu, Tassa Saldi, Katrina Diener, Ken Jones, Xiang-Dong Fu, David L. Bentley
GENES & DEVELOPMENT
(2014)
Article
Biochemistry & Molecular Biology
Kris Brannan, Hyunmin Kim, Benjamin Erickson, Kira Glover-Cutter, Soojin Kim, Nova Fong, Lauren Kiemele, Kirk Hansen, Richard Davis, Jens Lykke-Andersen, David L. Bentley
Review
Genetics & Heredity
David L. Bentley
NATURE REVIEWS GENETICS
(2014)
Article
Biochemistry & Molecular Biology
Stephane Larochelle, Ramon Amat, Kira Glover-Cutter, Miriam Sanso, Chao Zhang, Jasmina J. Allen, Kevan M. Shokat, David L. Bentley, Robert P. Fisher
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2012)
Article
Cell Biology
Brianna J. Klein, Lianhua Piao, Yuanxin Xi, Hector Rincon-Arano, Scott B. Rothbart, Danni Peng, Hong Wen, Connie Larson, Xi Zhang, Xia Zheng, Michael A. Cortazar, Pedro V. Pena, Anthony Mangan, David L. Bentley, Brian D. Strahl, Mark Groudine, Wei Li, Xiaobing Shi, Tatiana G. Kutateladze
Article
Oncology
Jessica Finlay-Schultz, Austin E. Gillen, Heather M. Brechbuhl, Joshua J. Ivie, Shawna B. Matthews, Britta M. Jacobsen, David L. Bentley, Peter Kabos, Carol A. Sartorius
Review
Biochemistry & Molecular Biology
Kris Brannan, David L. Bentley
GENETICS RESEARCH INTERNATIONAL
(2012)