Article
Pharmacology & Pharmacy
Yingying Luo, Jipeng Yin, Rutang Fang, Jingtao Liu, Lu Wang, Haiping Zhang, Ming Zhang, Zhijie Lei, Shuhui Liang, Wei Cui, Zhiyong Zhang, Kaichun Wu, Xiaoli Hui
Summary: The specificity of the peptide GX1 to retinal neovasculature has been confirmed, with dimeric GX1 showing enhanced antiangiogenic effects compared to the monomer. This suggests that dimeric GX1 could be a promising agent for antiangiogenic therapy in retinal angiogenesis-related diseases.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Chemistry, Applied
Fengchun Zhao, Ruirui Shi, Ruxia Liu, Yuan Tian, Zhengyou Yang
Summary: The review summarized the phage-display technique as a new method for producing antibody and antigen substitutes, as well as their applications and advantages in immunoassays.
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Microbiology
Ye Yang, Shuo Liu, Yufeng Luo, Bolun Wang, Junyi Wang, Juan Li, Jiaxin Li, Buqing Ye, Youchun Wang, Jianzhong Jeff Xi
Summary: We report a single-cell screening assay that allows rapid and high-throughput optimization of a human antibody displayed on a mammalian cell surface. Our approach can improve antibody affinity with an EC50 below 5 ng/mL within 2 weeks. This provides an effective strategy for fighting against evolving COVID variants.
Article
Multidisciplinary Sciences
Justina Rutkauskaite, Simon Berger, Stavros Stavrakis, Oliver Dressler, John Heyman, Xavier Casadevall i Solvas, Andrew deMello, Linas Mazutis
Summary: In this study, a droplet microfluidic approach was developed for high-throughput screening and sorting of antibody-secreting cells. The method utilizes Forster resonance electron transfer (FRET) signal to mediate the binding of antibodies and probes, enabling functional characterization of individual cells. The system efficiently screens antigen-specific cells and provides important data on antibody secretion rate and membrane-bound fraction.
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Ruiqi Huang, Gabrielle Warner Jenkins, Yunjeong Kim, Robyn L. Stanfield, Amrinder Singh, Maria Martinez-Yamout, Gerard J. Kroon, Jonathan L. Torres, Abigail M. Jackson, Abigail Kelley, Namir Shaabani, Baisen Zeng, Michael Bacica, Wen Chen, Christopher Warner, Jasmina Radoicic, Joongho Joh, Krishani Dinali Perera, Huldah Sang, Tae Kim, Jianxiu Yao, Fangzhu Zhao, Devin Sokd, Dennis R. Burton, Jeff Allen, William Harriman, Waithaka Mwangi, Donghoon Chung, John R. Teijaro, Andrew B. Ward, H. Jane Dyson, Peter E. Wright, Ian A. Wilson, Kyeong-Ok Chang, Duncan Mcgregor, Vaughn V. Smider
Summary: Antibodies derived from cows have the potential to be a new class of therapeutics, capable of neutralizing various SARS-CoV-2 variants and providing protection against disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Tianyang You, Yuan Ding, He Chen, Guangyue Song, Lianrun Huang, Minghua Wang, Xiude Hua
Summary: Competitive and noncompetitive immunoassays for clothianidin based on phage-displayed peptides were developed to improve sensitivity and specificity. The noncompetitive method showed the best specificity and sensitivity for clothianidin, with no cross-reactivity with analogs. The results demonstrated good recoveries and consistency with high-performance liquid chromatography in the analysis of blind samples.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Claire Milsted, Bo Dai, Nelson Garcia, Lu Yin, Yan He, Shahryar Kianian, Wojciech Pawlowski, Changbin Chen
Summary: This study used phage display to investigate the interactions of maize RAD51A1 with other proteins. The results revealed several peptide sequences that bind to ZmRAD51A1, supporting a potential role for ZmRAD51A1 in transcriptional regulation and plant defense.
Article
Biochemistry & Molecular Biology
K. M. Koss, T. Son, C. Li, Y. Hao, J. Cao, M. A. Churchward, Z. J. Zhang, J. A. Wertheim, R. Derda, K. G. Todd
Summary: Microglia play critical roles in the development and healthy function of the brain and spinal cord, but they are also involved in the pathology of various neuropsychiatric disorders. In this study, researchers explored the functional phenotypes of microglia and mixed microglia/astrocytes using in vitro models. They hypothesized that the biomolecular profile of these cells undergoes phenotypical changes, and they searched for novel peptide binders using a custom phage library. By studying the secretion of cytokines and nitric oxide and analyzing the expression of inducible nitric oxide synthase, they identified 58 unique peptides with potential functions related to glial support, inflammation activation, and regulation in neurodegenerative and neuropsychiatric disorders.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mouri R. J. Faruque, Nivedita Cukkemane, Cuicui Fu, Kamran Nazmi, Marja L. Laine, Floris J. Bikker
Summary: The objective of this study was to identify peptide sequences with specific selectivity for salivary MUC5B using phage display. The results showed that peptides MBP-12 and MBP-14 had the highest affinity to MUC5B and MBP-12 moderately stabilized the spinnbarkeit of mucous saliva. These findings suggest that combinatorial peptide libraries may contribute to the development of novel treatments for xerostomia.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Keitaro Yoshioka, Haruki Yamashita, Kazunori Shimizu, Sayako Shimomura, Takahiro Shibata, Jun-ichi Miyazaki, Hiroyuki Honda
Summary: This study identified new peptides with FFAR1 agonist activity as potential new drug candidates for treating type 2 diabetes mellitus. By utilizing phage display and physiochemical property prediction methods, peptides with high FFAR1 agonist activity can be screened. These findings provide new insights for drug development in treating type 2 diabetes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Mona Meng Wang, Chuanfei Chen, Myoe Naing Lynn, Carlos R. Figueiredo, Wei Jian Tan, Tong Seng Lim, Sarah E. Coupland, Anita Sook Yee Chan
Summary: UM is the most common primary adult intraocular malignancy, causing vision loss and poor survival rate. Current prognosis tools are based on tumor size, gene expression profile, and chromosomal rearrangements. However, preclinical evidence of metastasis and biomarkers for targeted therapy remain elusive.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Microbiology
Alison Felipe Alencar Chaves, Patricia Xander, Lavinia Maria Dal'Mas Romera, Fernando Luiz Affonso Fonseca, Wagner Luiz Batista
Summary: The global scenario of antimicrobial resistance is concerning, with slow progress in the development of new drugs. Difficulties in finding new targets for therapy, high development costs, and the mismatch between drug introduction and microbial adaptation are constraints in drug discovery.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ilana Cruz-Silva, Andrezza Justino Gozzo, Viviane Abreu Nunes, Aparecida Sadae Tanaka, Mariana da Silva Araujo
Summary: The study successfully cloned, expressed, and purified two recombinant CeEI isoinhibitors, which showed significant inhibitory effects on human neutrophil elastase, providing new insights for drug development targeting pulmonary disorders.
Review
Microbiology
Alison Felipe Alencar Chaves, Marina Valente Navarro, Yasmin Nascimento de Barros, Rafael Souza Silva, Patricia Xander, Wagner Luiz Batista
Summary: The dimorphic fungi of the Paracoccidioides genus are the causative agents of paracoccidioidomycosis (PCM), which is endemic in Latin America and primarily affects workers in rural areas. This fungus exhibits different morphologies at different temperatures and demonstrates strong adaptability to stress conditions within the host's internal environment.
Article
Biochemistry & Molecular Biology
Veronica Moraes Manzato, Ricardo Jose Soares Torquato, Francisco Jose Alves Lemos, Erika Nishiduka, Alexandre Keiji Tashima, Aparecida Sadae Tanaka
Summary: In Brazil, the main vector of arboviruses is Aedes aegypti. A pacifastin protease inhibitor library was constructed and a mutant, TiPI6, was successfully selected to inhibit proteolytic activities of Ae. aegypti larvae. TiPI6 can control all three digestive enzyme activities present in the larval midgut extract and may be a candidate for further larvicidal studies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Eloisa D. Castro, Pedro Paulo M. Mathias, Wagner L. Batista, Alex Yuri S. Sato, Mayte S. Toledo, Victor T. Almeida, Marli F. Curcio, Paulo E. Costa, Arnold Stern, Hugo P. Monteiro
Summary: The alternative splicing variant of NOS2, S3, plays a crucial role in regulating intracellular NO levels and cell viability in colon cancer cells. Inhibition of NOS2 S3 expression leads to higher intracellular NO levels, triggering autophagy and reducing cell viability. These findings highlight the potential significance of NOS2/NO-based therapies in colon cancer treatment.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Mycology
Giannina Ricci, Emeline Boni Campanini, Angela Satie Nishikaku, Rosana Puccia, Mariangela Marques, Ralf Bialek, Anderson Messias Rodrigues, Wagner Luiz Batista
Summary: This study identified six phylogenetic lineages belonging to the P. brasiliensis complex, with two lineages not grouping with any of the four recognized species and a coinfection involving two lineages. Additionally, the study found five parsimony-informative sites with potential non-synonymous substitutions that could lead to changes in the protein.
Article
Immunology
Camilla Ioshida Vasconcelos, A. Cronemberger-Andrade, Normanda Souza-Melo, Juliana Terzi Maricato, Patricia Xander, Wagner Luiz Batista, Rodrigo Pedro Soares, Sergio Schenkman, Ana Claudia Torrecilhas
Summary: This study evaluated the release and internalization of EVs from Trypanosoma cruzi under different stress conditions. It was found that EV release is dependent on membrane structure and parasite integrity, and stress conditions do not affect the functional properties of EVs during interaction with host cells. EVs released under stress conditions maintained their proinflammatory activity and stimulated the expression of certain genes in preactivated macrophages. Variations in EV release under stress conditions may be a physiological response against environmental changes.
JOURNAL OF IMMUNOLOGY RESEARCH
(2021)
Article
Microbiology
Marina Valente Navarro, Yasmin Nascimento de Barros, Wilson Dias Segura, Alison Felipe Alencar Chaves, Grasielle Pereira Jannuzzi, Karen Spadari Ferreira, Patricia Xander, Wagner Luiz Batista
Summary: The study revealed the crucial role of PbDrk1 in cell wall modulation of P. brasiliensis, affecting fungal resistance to different cell wall-disturbing agents and influencing cell wall morphogenesis by regulating various genes associated with cell wall modulation. It also showed an increased phagocytic index and proinflammatory cytokine levels in response to PbDrk1, suggesting its significance as a potential target for antifungal molecular activity.
Article
Biochemistry & Molecular Biology
Ilana Cruz-Silva, Viviane Abreu Nunes, Mariana Rydlewski, Andrezza Justino Gozzo, Priscila Praxedes-Garcia, Adriana Aparecida Ferraz Carbonel, Aparecida Sadae Tanaka, Mariana da Silva Araujo
Summary: Eczema is a skin condition characterized by itchy and inflammatory patches, with an imbalance between neutrophils and enzymes as a contributing factor. A mouse model utilizing neutrophil elastase (NE) was used to mimic eczema features, showing potential for protease inhibitor-based drugs in the management of this skin condition.
Article
Biochemistry & Molecular Biology
Larissa de Oliveira Passos Jesus, Aline Aparecida de Souza, Heron Fernandes Vieira Torquato, Vanessa Silva Gontijo, Rossimirian Pereira de Freitas, Tarsis Ferreira Gesteira, Vivien Jane Coulson-Thomas, Ricardo Jose Soares Torquato, Aparecida Sadae Tanaka, Edgar Julian Paredes-Gamero, Wagner Alves de Souza Judice
Summary: This study demonstrates the cytotoxic activity of alkyltriazole compounds against acute lymphoblastic lineages, making them a promising scaffold for the development of related molecules.
Article
Oncology
Maisoon Matareed, Eleftheria Maranou, Saara A. Koskela, Arfa Mehmood, Helen Kalirai, Sarah E. Coupland, Carlos R. Figueiredo
Summary: Loss of BAP1 gene expression in metastatic uveal melanoma triggers immunosuppressive mechanisms in the tumor microenvironment. PLIN2 expression serves as a prognostic biomarker in uveal melanoma. Loss of adipophilin expression is significantly associated with monosomy 3 status and nuclear BAP1 losses in uveal melanoma tumors.
JOURNAL OF PATHOLOGY
(2023)
Article
Immunology
Mona Meng Wang, Saara A. Koskela, Arfa Mehmood, Miriam Langguth, Eleftheria Maranou, Carlos R. Figueiredo
Summary: Immune Checkpoint Therapies (ICT) have greatly improved the treatment of metastatic melanoma, but not all patients respond well to it. Deficient beta 2-microglobulin (beta 2M) expression affects antigen presentation to T cells, leading to resistance to ICT. This study investigated alternative biomarkers associated with ICT resistance by analyzing transcriptomic expression and epigenetic control in melanoma patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Rafael de Souza Silva, Wilson Dias Segura, Reinaldo Souza Oliveira, Patricia Xander, Wagner Luiz Batista
Summary: Paracoccidioidomycosis (PCM) is a prevalent fungal infection in Latin America, caused by fungi from the Paracoccidioides genus. This study characterized the modulation of acid proteases in Paracoccidioides brasiliensis and found that these proteases are regulated by environmental conditions and fungal thermo-dimorphism. The findings provide new possibilities for studying the role of aspartyl proteases in the host-pathogen relationship and P. brasiliensis biology.
Article
Toxicology
Karla A. Oliveira, Ricardo J. S. Torquato, Daniela C. G. Garcia Lustosa, Tales Ribeiro, Bruno W. L. Nascimento, Lilian C. G. de Oliveira, Maria A. Juliano, Thaysa Paschoalin, Virginia S. Lemos, Ricardo N. Araujo, Marcos H. Pereira, Aparecida S. Tanaka
Summary: This study investigated the role of a serine protease activity found in the saliva of Triatoma infestans in vasomotor modulation, showing a preference for cleaving PAR peptides, particularly PAR-2. The serine protease induced vasodilation through NO release, with a significant effect on endothelium-dependent relaxation in vascular tissues, and was found to activate PAR-2 receptors.
JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES
(2021)