Article
Biochemistry & Molecular Biology
Paula Calvo, Antonia Costa-Bauza, Felix Grases
Summary: This study evaluated the effect of phytate on the crystallization of calcium oxalate, hydroxyapatite and brushite in artificial urine. The results showed that all lower InsPs seemed to inhibit the crystallization of calcium salts, especially InsP6 and InsP5 for calcium oxalate, and InsP5 and InsP4 for brushite. However, phytate had weak effects on the crystallization of hydroxyapatite in vitro, indicating a different mechanism for its in vivo crystallization.
Article
Microbiology
Bradley Benjamin, Angad Garg, Nikolaus Jork, Henning J. Jessen, Beate Schwer, Stewart Shuman
Summary: We studied the biochemical properties and mutations of the autonomous Asp1 kinase domain in fission yeast. The results showed that under optimal kinase conditions, the reaction of Asp1 kinase with IP6 and ATP resulted in both IP6 phosphorylation to 1-IP7 and hydrolysis of the ATP gamma-phosphate with near-equal partitioning between productive 1-IP7 synthesis and unproductive ATP hydrolysis.
Article
Biochemistry & Molecular Biology
Guangning Zong, Stephen B. Shears, Huanchen Wang
Summary: In this study, the biochemical and structural analysis of ITPKs from maize and soybeans were conducted, providing insights into the inositol phosphate kinases family in plants. By introducing a new enzyme activity assay and determining the crystal complex structure, the catalytic mechanism of these kinases was elucidated. The study discovered a signaling molecule related to phosphate homeostasis and revealed functional differences between different isoforms.
Review
Biochemistry & Molecular Biology
Becky Tu-Sekine, Sangwon F. Kim
Summary: Cells rely on nutrients to provide energy and carbon building blocks, and interact with the inositol phosphate signaling network to meet cellular needs in response to energy storage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Jae Hee Lee, Byeongwook Jeon, Mijeong Park, Jimin Ha, Soo Jung Kim, Mi Kwon Son, Seungho Wang, Joo Han Lee, Youn Kyoung Jeong
Summary: The study showed that BR101801, a DNA-PK inhibitor, can effectively enhance genomic instability induced by ionizing radiation and increase sensitivity in human solid cancer cells through multiple pathways. It affects factors in both the non-homologous end-joining repair pathway and homologous recombination.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Takashi Ushiki, Tomoharu Mochizuki, Katsuya Suzuki, Masami Kamimura, Hajime Ishiguro, Tatsuya Suwabe, Tomoyuki Kawase
Summary: This study found a correlation between ATP and polyphosphate (polyP), suggesting the involvement of ATP in the production of polyP in resting platelets of non-athletes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Olga Shadrina, Irina Garanina, Andrey Anisenko, Igor Kireev, Marina Gottikh
Summary: DNA-dependent protein kinase (DNA-PK) and its subunits, Ku70, Ku80, and DNA-PKcs, play important roles in various cellular processes. Depletion of these subunits results in different effects on gene expression, with Ku70 playing a more critical role in regulating multiple processes.
Article
Cell Biology
Yao Fan, Yi Liang, Yu Liu, Hui Fan
Summary: Hepatitis B virus (HBV) infection is a major global health problem due to its high risk of liver failure and hepatocellular carcinoma. This study identified PRKDC as an effective activator of HBV cccDNA transcription and revealed the mechanism through which it interacts with POLR2A and POLR2B to recruit RNA polymerase II (Pol II) to the HBV cccDNA minichromosome. The findings provide new insights into cccDNA transcription regulation and offer potential targets for HBV treatment.
CELL DEATH & DISEASE
(2022)
Article
Virology
Arushi Vats, Neva Skrabar, Giannino Del Sal, Lawrence Banks
Summary: This study demonstrates that knocking down the E6AP gene in cervical cancer cells leads to an increase in phosphorylation of the E6 oncoprotein. The phosphorylation of E6 requires the transcriptional activity of p53 and the presence of the enzyme DNA PK. This study defines a feedback loop where activation of p53 induces phosphorylation of E6, which in turn inhibits p53 transcriptional activity independently of E6's ability to degrade p53.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Roberto Docampo, Guozhong Huang
Summary: Trypanosoma cruzi and the T. brucei group of parasites are neglected diseases affecting millions of people globally. These microorganisms have complex life cycles involving insect vectors and mammalian hosts, and possess unique signaling pathways and receptor adaptations that differentiate them from their mammalian counterparts.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Oncology
Amr Ghaleb, Lucia Roa, Natalia Marchenko
Summary: The biological consequences of low-dose radiation (LDR) in breast cancer are unclear, while high-dose radiation (HDR) promotes DNA repair and cell cycle arrest. Mutant p53 promotes mammary tumorigenesis following LDR, but has negligible effects on tumors carrying the wild-type p53 allele.
Review
Immunology
Guy Tran Van Nhieu, Patricia Latour-Lambert, Jost Enninga
Summary: Shigella's type III effector IpgD plays a key role in disrupting the cortical actin filaments of epithelial cells by decreasing PI4,5P(2) levels, facilitating bacterial invasion and cell migration. Additionally, IpgD also stimulates various signaling pathways and regulates endosomal trafficking and immune responses, making it a versatile bacterial enzyme.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Liang Zhang, Jianong Zhang, Yan Liu, Pingzhao Zhang, Ji Nie, Rui Zhao, Qin Shi, Huiru Sun, Dongyue Jiao, Yingji Chen, Xiaying Zhao, Yan Huang, Yao Li, Jian-Yuan Zhao, Wei Xu, Shi-Min Zhao, Chenji Wang
Summary: STAT5A interacts with mitochondrial PDC to disrupt its integrity, impacting cellular glycolysis and oxidative phosphorylation. Increased mitochondrial translocation of STAT5A under hypoxia enhances the Warburg effect in cancer cells.
CELL DEATH & DISEASE
(2021)
Article
Veterinary Sciences
Peter Hekman, Johan Schefferlie, Ronette Gehring
Summary: The study investigated the impact of age-dependent pharmacokinetics (PK) on the clinical efficacy of oxytetracycline (OTC) in treating Bovine Respiratory Disease (BRD) in beef cattle. The results showed that the treatment outcomes varied in different dosing regimens and age groups.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Oncology
Cho R. Hong, Chantal D. Buckley, Way W. Wong, Praju Anekal, Benjamin D. Dickson, Gib Bogle, Kevin O. Hicks, Michael P. Hay, William R. Wilson
Summary: This study evaluated the radiosensitizing effects of the DNA-PK inhibitor AZD7648 on HNSCC cells, tumors, and normal tissues. The results demonstrated that AZD7648 had potent radiosensitizing effects on tumor cells and also significantly enhanced radiosensitivity in stem cells in the small intestine and oral mucosa.
RADIOTHERAPY AND ONCOLOGY
(2022)