Article
Environmental Sciences
Emad H. M. Hassanein, Basel A. Abdel-Wahab, Fares E. M. Ali, Omnia A. M. Abd El-Ghafar, Magy R. Kozman, Souty M. Z. Sharkawi
Summary: The study demonstrated that TFA has potential protective effects against CDDP-induced testicular injury by reducing oxidative stress and inflammatory response. It also enhanced the cytotoxic effect of CDDP in vitro through induction of apoptosis in both early and late stages. Additionally, TFA modulated various signaling pathways related to inflammation and cytotoxic activity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Zeinab Babaei, Mojtaba Panjehpour, Seyedeh Sara Ghorbanhosseini, Hadi Parsian, Mahsa Khademi, Mahmoud Aghaei
Summary: This study demonstrated the signaling pathways of VEGFR3 in ovarian cancer cells through upregulated expression of miR-1236. Inhibition of VEGFR3 using miR-1236 reduced cell proliferation, migration, and invasion ability, and increased apoptosis rate in SKOV3 and OVCAR3 cells. Additionally, VEGFR3 stimulation increased ERK1/2 and AKT phosphorylation, while VEGFR3 suppression resulted in decreased phosphorylation of ERK1/2 and AKT. These findings suggest that the miR-1236/VEGFR3 axis regulates ovarian cancer cell proliferation by modulating ERK1/2 and AKT signaling, providing a potential therapeutic strategy against ovarian cancer.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Neurosciences
Akihiro Ishii, Miki Furusho, Rashmi Bansal
Summary: Multiple intracellular signaling pathways, including Mek/ERK1/2-MAPK and PI3K/Akt/mTOR, are involved in regulating myelination in the peripheral nervous system. The roles of these pathways may differ during development and adulthood, highlighting the complexity of their interactions in Schwann cells.
Article
Biochemistry & Molecular Biology
Busra Karaca, Elcin Bakir, Mukerrem Betul Yerer, Ahmet Cumaoglu, Zuhal Hamurcu, Ayse Eken
Summary: The study revealed that doxazosin and erlotinib exhibited cytotoxic and apoptotic effects in human endometrial cancer cells, suggesting their potential therapeutic effects by modulating key gene expressions in signaling pathways.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Oncology
Xinghua Gao, Jikai Liu, Daming Fan, Xiaofeng Li, Zhiqing Fang, Keqiang Yan, Yidong Fan
Summary: This study found that berberine (BER) enhances gemcitabine (GEM) induced cytotoxicity in bladder cancer cells by downregulating the expression of RAD51 recombinase (Rad51) through inactivating the PI3K/Akt pathway. This provides a novel therapeutic target for bladder cancer treatment.
Article
Biochemistry & Molecular Biology
Ji-Eun Kim, Duk-Shin Lee, Hana Park, Tae-Hyun Kim, Tae-Cheon Kang
Summary: Our study demonstrates that AMPAR antagonists can restore impaired GRIA1 ubiquitination in responders by regulating the PP2B-ERK1/2-SGK1-NEDD4-2 signaling pathway, but not in non-responders. Co-treatment with CsA can improve the effects of AMPAR antagonists in non-responders, suggesting that dysregulation of PP2B-ERK1/2-SGK1-NEDD4-2-mediated GRIA1 ubiquitination may contribute to refractory seizures in epilepsy patients.
Article
Medicine, Research & Experimental
Jirapak Ruttanapattanakul, Nitwara Wikan, Siriporn Okonogi, Mingkwan Na Takuathung, Pensiri Buacheen, Pornsiri Pitchakarn, Saranyapin Potikanond, Wutigri Nimlamool
Summary: Boesenbergia rotunda extract promotes wound healing by stimulating cell proliferation, activating the ERK1/2 and Akt signaling pathways, but does not enhance cell migration. These findings support the potential of BR as a wound-healing promoting agent.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Anuradha Kirtonia, Amit Kumar Pandey, Balaji Ramachandran, Durga Prasad Mishra, David W. Dawson, Gautam Sethi, Trivadi S. Ganesan, H. Phillip Koeffler, Manoj Garg
Summary: LAMC2 plays a crucial role in the tumorigenesis of PDAC by repressing epithelial-mesenchymal transition and modulating the EGFR/ERK1/2/AKT/mTOR axis, thus inhibiting PDAC tumor growth and metastasis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Developmental Biology
Colin J. Dinsmore, Philippe Soriano
Summary: Understanding how cells activate intracellular signaling pathways in response to external signals is an important goal in cell and developmental biology. Live-cell signaling reporters have greatly contributed to our understanding of signaling dynamics. Translocation reporters have received attention due to their ease of use and fast response times. This study created two new mouse reporter lines to investigate the ERK1/2 and AKT signaling pathways.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yuxiang Song, Kexin Wang, Juan J. Loor, Qianming Jiang, Yuchen Yang, Shang Jiang, Siyuan Liu, Jiyuan He, Xiancheng Feng, Xiliang Du, Lin Lei, Wenwen Gao, Guowen Liu, Xinwei Li
Summary: This study investigated the effects of BHB on neutrophil apoptosis and the underlying regulatory mechanisms during ketosis. The results showed that high concentrations of BHB inhibit apoptosis in bovine neutrophils by activating the ERK1/2 and AKT signaling pathways. These findings have important implications for understanding systemic inflammation in ketotic cows.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Oncology
Aurimas Stulpinas, Matas Sereika, Aida Vitkeviciene, Ausra Imbrasaite, Natalija Krestnikova, Audrone V. V. Kalvelyte
Summary: Cell signaling manipulation is crucial for anticancer therapy, and the cell state determines the response to drugs. Therefore, understanding the relationship between cell state and therapeutic sensitivity is essential for the development of cancer treatments. Patient-derived ex vivo cell models show promise in translating research findings into clinical applications in the era of personalized medicine.
FRONTIERS IN ONCOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jinjun Wu, Muyan Kong, Yanmei Lou, Leyan Li, Chunlin Yang, Huifang Xu, Yuqi Cui, Hong Hao, Zhenguo Liu
Summary: This study found that FMNT significantly enhances endothelial function and promotes angiogenesis through activating Erk1/2- and Akt-mediated eNOS/NO signaling pathway. Simultaneous activation of Erk1/2 and Akt signaling is required for FMNT-induced promotion of endothelial function, providing evidence for potential clinical applications in conditions related to vascular insufficiency.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Radhika Adhikari, Jaehun Jung, Saugat Shiwakoti, Eun-Young Park, Hyun-Jung Kim, Ju-Young Ko, Jaeyoon You, Minho Lee, Min -Ho Oak
Summary: Calcific aortic valve stenosis (CAVS), the third most prevalent cardiovascular disorder, imposes a significant burden on patients but lacks pharmaceutical treatment options. Aortic valve replacement is the only option, but its effectiveness is uncertain and complications are common. Capsaicin, known for its anti-inflammatory and antioxidant properties, has been found to inhibit arterial calcification. In this study, capsaicin was shown to reduce calcium deposition in calcified aortic valve interstitial cells (VICs) and downregulate calcification markers, suggesting its potential as a treatment for CAVS. The mechanism of action involves inhibition of the NF kappa B/AKT/ERK1/2 signaling pathway, oxidative stress, and AGE-RAGE signaling pathways.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Farheen Badrealam Khan, Irfa Anwar, Elrashdy M. Redwan, Abdulrasheed Palakkott, Arshida Ashraf, Jaleel Kizhakkayil, Rabah Iratni, Sajid Maqsood, Mohammed Akli Ayoub
Summary: Lactoferrin has been found to have antidiabetic properties by activating insulin receptors and their downstream signaling pathways. This suggests that lactoferrin may be the bioactive component responsible for the antidiabetic properties of camel milk.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shiyu Hao, Chunyan Yang, Peng Song, Hewen Shi, Ying Zou, Meiyang Chen, Xingli Wu, Yancun Yin, Zhenhai Yu, Weiwei Zhu, Minjing Li
Summary: The tight junction protein CLDN4 is closely related to the development of acute myeloid leukemia (AML). Up-regulated expression of CLDN4 in AML cells is associated with decreased cell growth and increased apoptosis. Knockdown of CLDN4 inhibits the expression of PIK3R3 and MAP2K2, suppressing the activation of AKT and ERK1/2. Higher expression of CLDN4 is correlated with worse survival and shorter disease-free survival in AML patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Shao-Hua Wang, Chih-Yu Huang, Chun-Yen Chen, Chia-Che Chang, Chun-Yung Huang, Cheng-Di Dong, Jo-Shu Chang
Summary: Fucoidans, sulfated polysaccharides of marine origin, were extracted from brown macroalgae using microwave-assisted and ultrasound-assisted extraction methods. The purified fucoidan product showed nearly 84.0% purity and exhibited anti-lipogenesis activity with a 28.9% decrease in lipid synthesis compared to the control.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Jyh-Cheng Chen, Jen-Chung Ko, Yong-Cing Taso, Hsiang-Hung Cheng, Tzu-Ying Chen, Ting-Chuan Yen, Yun-Wei Lin
Summary: The study revealed the regulatory effect of bevacizumab on XPC protein expression, and the synergistic cytotoxic effect of 17-AAG in combination with bevacizumab. These results provide a rationale for combining bevacizumab with Hsp90 inhibitors to enhance therapeutic effects for lung cancer.
Article
Oncology
Sheng-Kai Liang, Li-Ta Keng, Chia-Hao Chang, Yueh-Feng Wen, Meng-Rui Lee, Ching-Yao Yang, Jann-Yuan Wang, Jen-Chung Ko, Jin-Yuan Shih, Chong-Jen Yu
Summary: The study suggests that afatinib may not provide longer overall survival compared to first-generation TKIs in patients with EGFR mutant lung adenocarcinoma, but may have a survival benefit in smokers. Pemetrexed may be the preferred choice for subsequent chemotherapy.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chun-Yen Chen, Shao-Hua Wang, Chih-Yu Huang, Cheng-Di Dong, Chun-Yung Huang, Chia-Che Chang, Jo-Shu Chang
Summary: This study found that the decrease in molecular mass of fucoidan extracted from brown seaweed can enhance its antioxidant activity, anti-lipogenesis, and anti-inflammatory activities. Additionally, the degree of sulfation of fucoidan also affects its antioxidant and anti-inflammatory capabilities.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Environmental Sciences
Yun-Wei Chiang, Chun-Hung Su, Han-Yin Sun, Shih-Pin Chen, Chun-Jung Chen, Wen-Ying Chen, Chia-Che Chang, Chuan-Mu Chen, Yu-Hsiang Kuan
Summary: BPA, an estrogen-like compound, can enter the human body, affecting ARPE-19 cells and leading to cytotoxicity.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Engineering, Chemical
En-Ting Lin, Yi-Chieh Lee, Hui-Min David Wang, Chen-Yaw Chiu, Yu-Kaung Chang, Chun-Yung Huang, Chia-Che Chang, Pei-Chien Tsai, Jo-Shu Chang
Summary: Fucoidan is a polysaccharide with excellent antioxidant, anti-inflammatory, anti-angiogenic, antitumor, and antibacterial capabilities. Subcritical water extraction is an effective method for extracting fucoidan and preserving its bioactivity.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Pharmacology & Pharmacy
Jen-Chung Ko, Jyh-Cheng Chen, Jou-Min Hsieh, Pei-Yu Tseng, Chen -Shan Chiang, Li -Ling Liu, Chin-Cheng Chien, I-Hsiang Huang, Qiao-Zhen Chang, Bo-Cheng Mu, Yun-Wei Lin
Summary: This study found that the Hsp90 inhibitor 17-AAG can enhance the cytotoxicity of tamoxifen and erlotinib in NSCLC cells by regulating TP expression.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Min-Yung Kuo, Wei-Ting Yang, Yann-Jen Ho, Ge-Man Chang, Hsiung-Hao Chang, Chao-Yu Hsu, Chia-Che Chang, Yi-Hsin Chen
Summary: Bladder cancer is a common malignancy worldwide. STAT3 is a transcription factor commonly hyperactivated in cancers, including bladder cancer. HME, a compound derived from a medicinal mushroom, was found to be cytotoxic to bladder cancer cells by blocking STAT3 activation and suppressing the downstream survivin signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Infectious Diseases
Chia-Hao Chang, Lih-Yu Chang, Jen-Chung Ko, Yueh-Feng Wen, Chien-Jen Chang, Li-Ta Keng, Ping-Hsien Tsou, Kai-Lun Yu, Jann-Yuan Wang, Chong-Jen Yu
Summary: This prospective cohort study investigates the incidence, risk factors, and renal outcomes of acute kidney injury (AKI) during anti-tuberculosis (TB) treatment. The study finds that older age, higher baseline estimated glomerular filtration rate (eGFR), and higher blood eosinophil count are associated with a higher risk of AKI during TB treatment. However, these complications do not impact TB treatment completion, and no permanent renal impairment occurs.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Chao-Yu Hsu, Wei-Ting Yang, Ju-Hwa Lin, Chien-Hsing Lu, Kai-Cheng Hu, Tsuo-Hung Lan, Chia-Che Chang
Summary: Sertindole has been found to have antiproliferative effects on bladder cancer cells by inhibiting the STAT3/BCL-xL signaling pathway, suggesting its potential as a therapeutic strategy for bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chih-Pin Liao, Ya-Chu Hsieh, Chien-Hsing Lu, Wen-Chi Dai, Wei-Ting Yang, Kur-Ta Cheng, Modukuri V. Ramani, Gottumukkala V. Subbaraju, Chia-Che Chang
Summary: This study presents the first report on the anti-TNBC effect and action mechanism of Methoxyhispolon Methyl Ether (MHME). It was found that MHME induces apoptosis in TNBC cells by inhibiting the activation of STAT3 and downregulating BCL-2 expression. Additionally, MHME suppresses SRC activation. These findings suggest the potential of MHME as a TNBC chemotherapy agent.
Article
Biochemistry & Molecular Biology
Ya-Chu Hsieh, Yuan-Chang Dai, Kur-Ta Cheng, Wei-Ting Yang, Modukuri V. Ramani, Gottumukkala V. Subbaraju, Yi-Ju Chen, Chia-Che Chang
Summary: Colorectal cancer (CRC) is the third most prevalent human cancer globally, and current chemotherapy has limited effectiveness. This study found that Dehydroxyhispolon methyl ether (DHME) has a strong cytotoxic effect on CRC cells while being less toxic to normal colon epithelial cells. DHME induces CRC cell apoptosis by blocking the SRC/STAT3/BCL-2 axis, suggesting its potential as a CRC chemotherapy agent.
Article
Toxicology
Jen-Chung Ko, Jyh-Cheng Chen, Pei-Yu Tseng, Jou-Min Hsieh, Chen-Shan Chiang, Li-Ling Liu, Chin-Cheng Chien, I-Hsiang Huang, Yun-Wei Lin
Summary: The combination therapy of nitroglycerin (NTG) and multitargeted antifolate agent pemetrexed shows synergistic cytotoxicity and cell growth inhibition against non-small-cell lung cancer (NSCLC) cells. This effect is attributed to the downregulation of RAD52 and inactivation of AKT and ERK1/2, leading to enhanced DNA repair suppression and cell growth inhibition.
TOXICOLOGY RESEARCH
(2022)
Article
Food Science & Technology
Chang-Ming Chern, Chung-Kuang Lu, Kuo-Tong Liou, Yea-Hwey Wang, Keng-Chang Tsai, Chia-Lin Chang, Chia-Che Chang, Yuh-Chiang Shen
Summary: This study demonstrates the neuroprotective effect of medicarpin on stroke-induced injury, reducing brain infarction, protecting the blood-brain barrier, and promoting brain repair through stimulation of neurogenesis and expression of neuroprotective proteins.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2021)
Review
Biotechnology & Applied Microbiology
Yu-Chen Liao, Chia-Che Chang, Dillirani Nagarajan, Chun-Yen Chen, Jo-Shu Chang
Summary: Hydrocolloids are a type of food additives widely used in the food industry to develop structure in food ingredients. Algae-derived hydrocolloids such as carrageenan, agar, and alginate are commonly used as thickening, gelling, and emulsifying agents. Despite the debate surrounding carrageenan, algal hydrocolloids are considered a safe alternative to synthetic additives.