Article
Biochemistry & Molecular Biology
Xumei Liu, Qiong Shi, Namrta Choudhry, Ting Zhang, Hong Liu, Shenqiu Zhang, Jing Zhang, Dun Yang
Summary: Small molecule inhibitors of aurora kinases are being investigated for their potential role in cancer treatment. This study focused on the effects of a reversible pan-aurora kinase inhibitor, VX-680, on normal human cells. The researchers found that VX-680 could block cell division and induce cell arrest, but a fraction of normal cells were able to resume proliferation after treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jie Dong, Liping Jin, Shihua Bao, Biaobang Chen, Yang Zeng, Yuxi Luo, Xingzhu Du, Qing Sang, Tianyu Wu, Lei Wang
Summary: Aneuploidy, a condition where cells have an abnormal number of chromosomes, significantly affects female fertility and increases the likelihood of birth defects. This study found that the ectopic expression of a specific human gene, TUBB8, in mouse oocytes leads to higher rates of aneuploidy by causing defects in the attachment of kinetochore-microtubules. The study also identified variants in the C-terminal tail of TUBB8 in patients with recurrent miscarriages, further implicating the gene in reproductive complications. This research provides new insights into the mechanisms underlying aneuploidy in human eggs and recurrent miscarriages.
Article
Oncology
Sara Lazaro, Corina Lorz, Ana Belen Enguita, Ivan Seller, Jesus M. Paramio, Mirentxu Santos
Summary: Lung cancer is a leading cause of cancer death worldwide. This study investigates the impact of deleting tumor suppressor genes in adult mice's lungs and identifies a new mouse model for pulmonary sarcomatoid carcinoma (PSC). PSC is rare and lacks effective treatment and preclinical models, making this discovery significant for further research and understanding the relationship between different types of non-small cell lung cancer.
Article
Biochemistry & Molecular Biology
Francesco Bellanti, Domenica Mangieri, Giorgia di Bello, Aurelio Lo Buglio, Giuseppe Pannone, Maria Carmela Pedicillo, Alberto Fersini, Michal Dobrakowski, Aleksandra Kasperczyk, Slawomir Kasperczyk, Gianluigi Vendemiale
Summary: Redox homeostasis plays an important role in the modulation of stem cell fate. This study investigated the impact of redox modifications on the differentiation potential of a human hepatic progenitor cell line (HepaRG). The results reveal that both intra- and extracellular redox balance are crucial in determining the fate of HepaRG cells, highlighting the significance of redox status in pre-clinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Junhua Gong, Minghua Cong, Hao Wu, Menghao Wang, He Bai, Jingyuan Wang, Keting Que, Kaiwen Zheng, Wenfeng Zhang, Xiaoli Yang, Jianping Gong, Hanping Shi, Mingyong Miao, Fangchao Yuan
Summary: The study investigates the role of the p53/miR-34a/SIRT1 positive feedback loop in the termination of liver regeneration and its potential relationship with liver cancer. The overexpression of p53 or miR-34a terminates liver regeneration early and enhances the proapoptotic effect of the positive feedback loop. Additionally, the positive feedback loop is inhibited in hepatocellular carcinoma (HCC) patients with P53 mutations.
Article
Oncology
Kazumasa Aoyama, Daisuke Shinoda, Emi Suzuki, Yaeko Nakajima-Takagi, Motohiko Oshima, Shuhei Koide, Ola Rizq, Sha Si, Shiro Tara, Goro Sashida, Atsushi Iwama
Summary: Deletion of Ezh1 and Ezh2 leads to PRC2 insufficiency, causing dyserythropoiesis and activating the p53 pathway by de-repressing the Cdkn2a gene, contributing to disease progression. Rescuing dyserythropoiesis can be achieved by deleting Cdkn2a and p53.
Article
Biology
Abby Sarkar, Yinhua Jin, Brian C. DeFelice, Catriona Y. Logan, Yan Yang, Teni Anbarchian, Peng Wu, Maurizio Morri, Norma F. Neff, Huy Nguyen, Eric Rulifson, Matthew Fish, Avi Gurion Kaye, Azalia M. Martinez Jaimes, Roel Nusse, Pramod Mistry
Summary: Intermittent fasting promotes rapid hepatocyte proliferation and maintains liver homeostasis. This study challenges the widely held view that liver tissue is mostly quiescent and provides the first example of dietary influence on adult hepatocyte proliferation.
Article
Cell Biology
Kevin Hoffseth, Emily Busse, Josue Jaramillo, Jennifer Simkin, Michelle Lacey, Mimi C. Sammarco
Summary: Mouse digit amputation serves as a useful model for studying bone growth after injury, showing that intramembranous bone formation is promoted in adult animals. Aged mice display altered trabecular spacing and patterning, as well as increased mineral density in the regenerated bone. Analysis of elasticity using imaging techniques suggests that regenerated bone has decreased elasticity compared to uninjured bone, but no significant difference in elasticity between aged and young regenerated bone.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Graham S. Jackson, Jacqueline Linehan, Sebastian Brandner, Emmanuel A. Asante, Jonathan D. F. Wadsworth, John Collinge
Summary: Transgenic mice have provided valuable insights into the understanding of human prion diseases and the study of protein misfolding in neurodegenerative diseases. Overexpression of the wild-type mouse prion protein can cause a protein misfolding disorder similar to authentic prion disease.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Yuan Du, Wencheng Zhang, Hua Qiu, Canjun Xiao, Jun Shi, Lola M. Reid, Zhiying He
Summary: Murine models have genetic and physiological similarities with humans, making them valuable tools for studying biological processes and diseases. They are particularly advantageous for studying liver-related functions and diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuan Wang, Huazhang Wu, Nannan Dong, Xu Su, Mingxiu Duan, Yaqin Wei, Jun Wei, Gaofeng Liu, Qingjie Peng, Yunli Zhao
Summary: The study demonstrated that SFN impairs colony-forming ability in GC cells, suppresses cell proliferation by arresting cell cycle at S phase, enhances cell apoptosis, and upregulates p53, p21 while downregulating CDK2 expression, suggesting a potential therapeutic application of SFN in GC treatment and prevention.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Stephen R. Wellard, Yujiao Zhang, Chris Shults, Xueqi Zhao, Matthew McKay, Stephen A. Murray, Philip W. Jordan
Summary: The study highlights the critical role of centriole duplication, centrosome maturation, and separation in establishing bipolar spindles during meiotic divisions. The findings show that Polo-like kinase 1 and Aurora A kinase are essential for centrosome maturation and separation, and PLK1 is required to inhibit the second round of centriole duplication until late anaphase I to ensure accurate chromosome segregation during spermatogenesis.
Article
Cell Biology
Yao Xiao, Lantao Peng, Hongjuan Xu, Ming Huang, Chao Yang, Guodong Liu, Xiwu Ouyang, Xiaoli Li, Yuanjing Wang, Langqing Sheng, Denggao Zhai, Ling Lin, Ling Liu, Gang Liu, Liansheng Gong
Summary: Liver cancer is a highly lethal tumor and surgical resection is the main treatment. Insufficient residual liver volume after hepatectomy often leads to liver failure, posing a challenge in liver surgery. Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) has been shown to effectively reduce the occurrence of liver failure by inducing rapid proliferation of residual liver tissue. However, the molecular mechanism of residual liver tissue regeneration after primary ALPPS remains unknown.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mikhail L. Vorobev, Bashar A. Alhasan, Irina I. Suvorova
Summary: Autophagy plays a critical role in early embryonic development, particularly in blastocyst formation. The absence of p53 does not affect autophagy activation in pluripotent cells but hinders differentiation induced by retinoic acid. Despite p53 deletion, Ulk1-dependent autophagy is still activated in response to serum deprivation in mESCs, but impaired during differentiation induced by retinoic acid.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Xiaoqing Qian, Rui Ma, Xinwei Wang, Xinda Xu, Juanmei Yang, Fanglu Chi, Dongdong Ren
Summary: The study found that inducing injury and overexpressing Atoh1 can effectively promote hair cell regeneration, leading to the formation of more developed hair cell subtypes and providing a promising source of hair cells in neonatal vestibular organs.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Chi-Hsiao Yeh, Zhao-Qing Shen, Shao-Yu Hsiung, Pei-Chun Wu, Yuan-Chi Teng, Yi-Ju Chou, Su-Wen Fang, Chian-Feng Chen, Yu-Ting Yan, Lung-Sen Kao, Cheng-Heng Kao, Ting-Fen Tsai
Article
Oncology
Yi-Fan Chen, Tzu-Yu Chou, I-Hsuan Lin, Chung-Guang Chen, Cheng-Heng Kao, Guo-Jen Huang, Liang-Kung Chen, Pei-Ning Wang, Ching-Po Lin, Ting-Fen Tsai
JOURNAL OF PATHOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Zhao-Qing Shen, Yi-Long Huang, Yuan-Chi Teng, Tai-Wen Wang, Cheng-Heng Kao, Chi-Hsiao Yeh, Ting-Fen Tsai
Summary: CISD2 protein plays a crucial role in maintaining cellular homeostasis, and it has been identified as a pro-longevity gene, while also being down-regulated in natural aging. It is associated with cell survival and control of cell death.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yi-Long Huang, Zhao-Qing Shen, Chen-Hua Huang, Yuan-Chi Teng, Chao-Hsiung Lin, Ting-Fen Tsai
Summary: This study demonstrates that Cisd2 dosage modulates the severity of NAFLD induced by Western diet, with haploinsufficiency exacerbating the progression to NASH and increased copy number attenuating liver pathogenesis. Transcriptomic analysis reveals significant changes in biological processes related to inflammation, lipid metabolism, and DNA replication/repair, suggesting potential therapeutic targets for WD-induced fatty liver diseases.
Article
Cell Biology
Ning- Yang, Chi-Hsiao Yeh, Tsung-Hsien Tsai, Yi-Ju Chou, Paul Wei-Che Hsu, Chun-Hsien Li, Yun-Hsuan Chan, Li-Tang Kuo, Chun-Tai Mao, Yu-Chiau Shyu, Ming-Jui Hung, Chi-Chun Lai, Huey-Kang Sytwu, Ting-Fen Tsai
Summary: The study demonstrates that an AI-assisted identification of SNP signatures combined with clinical parameters is effective in identifying asymptomatic high-risk individuals predisposed to HF and predicting HF progression.
Article
Biochemistry & Molecular Biology
Chen-Hua Huang, Yi-Long Huang, Zhao-Qing Shen, Chao-Hsiung Lin, Ting-Fen Tsai
Summary: Cisd2 protects the liver from age-related fat accumulation, ameliorates dysregulation of lipid metabolism and protein homeostasis, and is considered a novel molecular target for therapies targeting fatty liver diseases. It is likely to prevent malignant progression to cirrhosis and hepatocellular carcinoma.
Article
Cell Biology
Yi-Long Huang, Zhao-Qing Shen, Chen-Hua Huang, Chao-Hsiung Lin, Ting-Fen Tsai
Summary: The liver plays a crucial role in mammalian aging, and the decrease in Cisd2 protein levels in the livers of old male mice may lead to non-alcoholic fatty liver disease. Maintaining high levels of Cisd2 protein can attenuate age-related dysregulation in lipid metabolism and other pathologies, while also protecting the liver from oxidative stress and mitochondrial DNA deletions. This suggests that enhancing Cisd2 expression could be a promising target for therapeutic interventions to slow down liver aging.
Article
Biochemistry & Molecular Biology
Yi-Long Huang, Chao-Hsiung Lin, Tsung-Hsien Tsai, Chen-Hua Huang, Jie-Ling Li, Liang-Kung Chen, Chun-Hsien Li, Ting-Fen Tsai, Pei-Ning Wang
Summary: This study utilized untargeted metabolomics analysis to characterize plasma metabolites in MCI patients progressing to AD and stable MCI patients. A 20-metabolite signature panel associated with the presence and progression of AD was identified using machine learning, offering predictive value for conversion from MCI to AD. Additionally, the bacteria-generated metabolite indole-3-propionic acid was identified as a predictor of AD progression, suggesting a role for gut microbiota in AD pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Chi-Chin Sun, Shao-Yun Lee, Cheng-Heng Kao, Li-Hsien Chen, Zhao-Qing Shen, Chia-Hui Lai, Tsai-Yu Tzeng, Jong-Hwei Su Pang, Wen-Tai Chiu, Ting-Fen Tsai
Summary: The findings illustrate the crucial role of Cisd2 in the cornea and suggest that targeting the Ca2+ signaling pathway could be a potential therapeutic strategy for corneal epithelial diseases.
Article
Biochemistry & Molecular Biology
Chi-Hsiao Yeh, Yi-Ju Chou, Tsung-Hsien Tsai, Paul Wei-Che Hsu, Chun-Hsien Li, Yun-Hsuan Chan, Shih-Feng Tsai, Soh-Ching Ng, Kuei-Mei Chou, Yu-Ching Lin, Yu-Hsiang Juan, Tieh-Cheng Fu, Chi-Chun Lai, Huey-Kang Sytwu, Ting-Fen Tsai
Summary: A study in Taiwan found a high proportion of clopidogrel resistance among diabetic PAD patients, suggesting that platelet function test-guided therapy could reduce adverse events. AI-assisted genome-wide association study identified a SNP signature composed of 20 SNPs in 9 protein-coding genes, revealing a protein interaction network related to the endocytosis and recycling of the drug target of clopidogrel. The findings suggest potential for developing precision medicine for antiplatelet therapy in diabetic PAD patients.
Letter
Endocrinology & Metabolism
I-Wen Wu, Tsung-Hsien Tsai, Chi-Jen Lo, Yi-Ju Chou, Chi-Hsiao Yeh, Mei-Ling Cheng, Chi-Chun Lai, Huey-Kang Sytwu, Ting-Fen Tsai
Article
Health Care Sciences & Services
I-Wen Wu, Tsung-Hsien Tsai, Chi-Jen Lo, Yi-Ju Chou, Chi-Hsiao Yeh, Yun-Hsuan Chan, Jun-Hong Chen, Paul Wei-Che Hsu, Heng-Chih Pan, Heng-Jung Hsu, Chun-Yu Chen, Chin-Chan Lee, Yu-Chiau Shyu, Chih-Lang Lin, Mei-Ling Cheng, Chi-Chun Lai, Huey-Kang Sytwu, Ting-Fen Tsai
Summary: AI-assisted models using machine learning algorithms were developed to identify biomarker signatures for diabetic kidney disease. The study reveals molecular mechanisms underlying the interaction between diabetes and chronic kidney disease, improving precision in diagnosing renal impairment.
NPJ DIGITAL MEDICINE
(2022)
Article
Cell Biology
Chi-Hsiao Yeh, Zhao-Qing Shen, Tai-Wen Wang, Cheng-Heng Kao, Yuan-Chi Teng, Teng-Kuang Yeh, Chung-Kuang Lu, Ting-Fen Tsai
Summary: This study found that hesperetin, a promising Cisd2 activator, when orally administered late in life, enhances Cisd2 expression and prolongs healthspan in old mice. Hesperetin functions mainly in a Cisd2-dependent manner to ameliorate age-related metabolic decline, body composition changes, glucose dysregulation, and organ senescence. Furthermore, a youthful transcriptome pattern is regained after hesperetin treatment during old age.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chi-Hsiao Yeh, Yi-Ju Chou, Ting-Kuan Chu, Ting-Fen Tsai
Summary: The study demonstrates that inducing cardiac-specific Cisd2 overexpression (Cisd2 icOE) at a late-life stage can prevent age-associated cardiac dysfunction, improve cardiac structure, and modulate aging-related pathways, rejuvenating the heart as it enters old age.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yi-Long Huang, Zhao-Qing Shen, Chia-Yu Wu, Yuan-Chi Teng, Chen-Chung Liao, Cheng-Heng Kao, Liang-Kung Chen, Chao-Hsiung Lin, Ting-Fen Tsai
