Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Shengnan Wang, Danni Qu, Guilan Zhao, Lina Yang, Lijie Zhu, Hong Song, He Liu
Summary: The microstructure and properties of the soy hull polysaccharide interfacial layer are significantly affected by pH, with higher stability at high pH levels but increased structural order at low pH levels. Low pH reduces thermal stability and increases viscosity of the emulsion, while high pH leads to higher viscosity.
Article
Chemistry, Physical
Kaili Zhu, Jun Ma, Liang Chen, Fangfang Wu, Xudong Xu, Mengqiu Xu, Wei Ye, Yao Wang, Peng Gao, Yujie Xiong
Summary: By designing the catalyst based on the interfacial water structure, this study achieved high activity and selectivity in electrochemical semihydrogenation. Experimental results showed that PdFe nanosheets could continuously produce a large amount of alkynes for 264 hours, demonstrating significant advantages over traditional thermal semihydrogenation methods in terms of performance, energy, and cost.
Article
Spectroscopy
M. Kopec, M. Blaszczyk, M. Radek, H. Abramczyk
Summary: This study evaluates the Raman biomarkers to distinguish tumor types, demonstrating that specific frequency bands in the spectra can serve as universal biomarkers for assessing the aggressiveness of human brain tumors. The clinical potential of Raman spectroscopy in oncological diagnostics is confirmed by the presented results.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Joao L. Lagarto, Enrico Baria, Melis Goktas, Patrick Igoche Onoja, Kerstin G. Blank, Francesco S. Pavone, Juergen Popp, Christoph Krafft, Riccardo Cicchi
Summary: The study demonstrates the potential of nondestructive optical imaging techniques such as SHG, RS, and FLIM in monitoring collagen degradation and assessing mechanical properties in tissues. These techniques can provide label-free monitoring of collagen degradation and rough evaluation of mechanical properties in a nondestructive manner.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Darena Schymanski, Barbara E. Ossmann, Nizar Benismail, Kada Boukerma, Gerald Dallmann, Elisabeth von der Esch, Dieter Fischer, Franziska Fischer, Douglas Gilliland, Karl Glas, Thomas Hofmann, Andrea Kappler, Silvia Lacorte, Julie Marco, Maria E. L. Rakwe, Jana Weisser, Cordula Witzig, Nicole Zumbulte, Natalia P. Ivleva
Summary: This study uses spectroscopic methods to identify and quantify microplastic particles, aiming to enhance the reliability of analysis, improve sample preparation, detection planning, and facilitate better understanding of existing research evaluations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Ying Li, Yanyan Wu, Mengke Yuan, Hongru Hao, Zhe Lv, Lingling Xu, Bo Wei
Summary: In this study, operando spectroscopy techniques were used to investigate the surface reconstruction behavior of FeOOH@NiFe layered double hydroxides (LDH) catalysts during the oxygen evolution reaction (OER). It was found that FeOOH@NiFe LDH transformed into highly active FeOOH@β-Ni(Fe)OOH phases. The FeOOH@NiFe LDH interface promoted interfacial charge transfer, delayed Ni oxidation, and induced the transformation of NiFe LDH phases into highly reactive β-Ni(Fe)OOH. The FeOOH@β-Ni(Fe)OOH exhibited excellent OER performance.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2022)
Article
Biochemistry & Molecular Biology
Szymon Tott, Marek Grosicki, Jacek Glowacz, Tasnim Mohaissen, Kamila Wojnar-Lason, Stefan Chlopicki, Malgorzata Baranska
Summary: Raman spectroscopy was utilized to characterize the biochemical phenotype of primary murine endothelial cells. The findings showed an increased protein content in heart EC and increased lipid content in lung EC in mice with heart failure. No organ-specific features were observed using Raman-based analysis of freshly isolated primary EC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Multidisciplinary Sciences
Czeslawa Paluszkiewicz, Maciej Roman, Natalia Piergies, Ewa Pieta, Monika Wozniak, Mariangela Cestelli Guidi, Katarzyna Miskiewicz-Orczyk, Magdalena Markow, Wojciech Scierski, Maciej Misiolek, Bogna Drozdzowska, Wojciech M. Kwiatek
Summary: This study investigated the feasibility of using FT-IR and RS methods to explore biochemical alterations in tumors, revealing significant differences in lipid, protein, carbohydrate, and nucleic acid content between healthy and tumoral tissues. Additionally, a novel approach to eliminate the influence of lipids on the elucidation of biochemical changes was proposed.
SCIENTIFIC REPORTS
(2021)
Article
Spectroscopy
Sylwia Terpilowska, Ewa Pieta, Maciej Roman, Czeslawa Paluszkiewicz, Wojciech M. Kwiatek
Summary: This study investigates the cellular response to various transition metals at the single-cell level using Raman and Fourier-transform infrared spectroscopic imaging. The results show that these transition metals can induce apoptosis and alter cellular metabolism at specific concentrations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Oncology
Monica Marro, Anna M. Rodriguez-Rivero, Cuauhtemoc Araujo-Andrade, Maria Teresa Fernandez-Figueras, Laia Perez-Roca, Eva Castella, Jordi Navines, Antonio Mariscal, Joan Francesc Julian, Pau Turon, Pablo Loza-Alvarez
Summary: In this study, the biochemical composition of breast cancer microcalcifications was investigated, revealing the natural adsorption and encapsulation of DNA within hydroxyapatite formations in breast cancer tissue. Other relevant biomolecules such as lipids, proteins, cytochrome C, and polysaccharides were also found to be encapsulated in these calcifications, representing an important breakthrough in understanding the role of hydroxyapatite in malignant tissues.
Article
Biochemistry & Molecular Biology
Michele Di Foggia, Carla Boga, Gabriele Micheletti, Benedetta Nocentini, Paola Taddei
Summary: Many restoring formulations for damaged hair keratin have been developed, claiming repair occurs through the reconstruction of disulfide bridges of keratin. Experimental results showed that shikimic acid and maleate-based restoring agents interacted with hair fibers, modifying the hair structure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Susniak, Mikolaj Krysa, Dominika Kidaj, Monika Szymanska-Chargot, Iwona Komaniecka, Katarzyna Zamlynska, Adam Choma, Jerzy Wielbo, Leopold L. Ilag, Anna Sroka-Bartnicka
Summary: Multimodal spectroscopic imaging methods were used to monitor the changes in distribution of selected metabolites involved in nitrogen fixation in pea root nodules, showing that biofertilizer containing Nod factors enhanced growth of pea plants and caused changes in leghaemoglobin distribution. Enhanced nitrogen fixation and accelerated maturation of nodules were observed with the use of biofertilizer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Santosh Kumar Paidi, Joel Rodriguez Troncoso, Mason G. Harper, Zhenhui Liu, Khue G. Nguyen, Sruthi Ravindranathan, Lisa Rebello, David E. Lee, Jesse D. Ivers, David A. Zaharoff, Narasimhan Rajaram, Ishan Barman
Summary: The use of Raman spectroscopy and machine learning allows for the identification of metastatic phenotype in tumors. Spectral analysis of tumors can predict their metastatic potential and evaluate the risk of metastasis in primary tumor biopsies of clinical patients.
Article
Biochemistry & Molecular Biology
Shuang Wang, Heping Li, Yu Ren, Fan Yu, Dongliang Song, Lizhe Zhu, Shibo Yu, Siyuan Jiang, Haishan Zeng
Summary: The study utilized Confocal Raman microspectral imaging to detect the pathological features of ductal carcinoma in situ (DCIS) and lobular hyperplasia (LH) in breast tissues compared to healthy tissue. The peak intensity variations in all three tissue types were evaluated using one-way ANOVA and Tukey's HSD post hoc tests, while linear discrimination analysis and principal component analysis were employed to classify spectral features and visualize histopathological variations. Spectral datasets were obtained and reconstructed for further pathologic visualization using multivariate imaging methods, providing detailed insights into the malignant transformation of breast cancer.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Oncology
Halina Abramczyk, Beata Brozek-Pluska, Monika Kopec, Jakub Surmacki, Maciej Blaszczyk, Maciej Radek
Summary: This study demonstrated the impact of cancerogenesis on the redox status of mitochondrial cytochromes in human breast cancer and brain tumors using Raman spectroscopy and imaging. The correlation between cytochromes Raman bands intensity and malignancy grade was shown. Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer for noninvasive grading and differential diagnosis.
Article
Biochemistry & Molecular Biology
Julia B. Krajewska, Jakub Wlodarczyk, Damian Jacenik, Radzislaw Kordek, Przemyslaw Taciak, Remigiusz Szczepaniak, Jakub Fichna
Summary: Inflammatory bowel diseases (IBD) are among the most common gastrointestinal diseases worldwide, yet effective and side-effect-free treatments are currently lacking. This study introduced a new concept for anti-inflammatory treatment utilizing gold (III) complexes and demonstrated the therapeutic potential of these complexes in treating IBD through in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hubert Zatorski, Maciej Salaga, Marta Zielinska, Kinga Majchrzak, Agata Binienda, Radzislaw Kordek, Ewa Malecka-Panas, Jakub Fichna
Summary: The study demonstrated that activation of AdipoR1 and AdipoR2 with the agonist AdipoRon reduces the occurrence of gastric ulcers by exerting anti-inflammatory and antioxidative effects, thus alleviating gastric tissue damage.
Article
Medicine, General & Internal
Renata Krupa, Wojciech Malecki, Piotr Czarny, Justyna Strycharz, Maciej Jablkowski, Radzislaw Kordek, Janusz Szemraj, Tomasz Sliwinski
Summary: The study investigated the potential of miRNAs regulating genes involved in iron homeostasis as diagnostic or prognostic factors for HCV-related hepatocellular carcinoma. It found that miRNA expression profiles were altered in HCC patients, with some miRNAs showing potential as diagnostic factors by correlating with iron metabolism proteins.ROC curve analyses showed that 15 miRNAs could effectively discriminate between HCC patients and healthy controls, with higher sensitivity and specificity than alpha-fetoprotein.
ARCHIVES OF MEDICAL SCIENCE
(2021)
Article
Nutrition & Dietetics
Maciej Salaga, Adrian Bartoszek, Agata Binienda, Julia B. Krajewska, Adam Fabisiak, Paula Mosinska, Katarzyna Dziedziczak, Karolina Niewinna, Marcin Talar, Aleksandra Tarasiuk, Radzislaw Kordek, Jakub Fichna
Summary: This study characterized the anti-inflammatory activity and effect on intestinal barrier function of synthetic FFAR agonists in mouse models of colitis. The FFAR4 agonist GSK137647 attenuated colitis and restored tight junction gene expression in the colon, indicating a potential therapeutic target for intestinal inflammation.
Article
Oncology
Halina Abramczyk, Jakub Maciej Surmacki, Beata Brozek-Pluska, Monika Kopec
Summary: Research using Raman scattering revealed a correlation between the redox status of cytochromes in cancer cells and cancer aggressiveness, with increased reduced cytochrome c concentration observed in cancer tissues.
Article
Biochemistry & Molecular Biology
Agata Binienda, Adam Makaro, Marcin Talar, Julia B. Krajewska, Aleksandra Tarasiuk, Adrian Bartoszek, Adam Fabisiak, Paula Mosinska, Karolina Niewinna, Katarzyna Dziedziczak, Mikolaj Swierczynski, Radzislaw Kordek, Maciej Salaga, Jakub Fichna
Summary: The study examined the potential interaction between opioid receptors (ORs) and free fatty acid receptors (FFARs) ligands in colitis using a mouse model. The results showed that treatment with ORs and FFARs ligands could impact immune cells in inflammation, however, no significant influence on the severity of colitis or synergistic effect was observed.
Article
Oncology
Wojciech Kuncman, Magdalena Orzechowska, Lukasz Kuncman, Radzislaw Kordek, Katarzyna Taran
Summary: This study aimed to identify the expression profiles of biomarkers in breast cancer, revealing differences and heterogeneity between primary tumors and axillary lymph node metastases, which have significant implications for the clinical subtype and characteristics of breast cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Physical
Halina Abramczyk, Jakub Maciej Surmacki, Beata Brozek-Pluska
Summary: This study presents a non-invasive approach to study the redox status of cytochromes in brain tumors and breast cancers using Raman spectroscopy in in vitro and in vivo models. The results show that the amount of reduced cytochrome is abnormally high in cancer cells and lower in normal cells. Additionally, mitochondrial dysfunction and alterations in chemical composition were detected through Raman imaging.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Jakub Maciej Surmacki, Isabel Quiros-Gonzalez, Sarah Elizabeth Bohndiek
Summary: This study demonstrates the capability of Raman spectroscopy in monitoring redox biochemical changes in cells under different antioxidant and pro-oxidant environments. The results show that Raman spectroscopy can accurately characterize the intracellular conditions associated with oxidative stress, providing valuable insights for further research in this area.
Article
Chemistry, Physical
Pawel Krzyczmonik, Marta Klisowska, Andrzej Leniart, Katarzyna Ranoszek-Soliwoda, Jakub Surmacki, Karolina Beton-Mysur, Beata Brozek-Pluska
Summary: The research aimed to develop a conductive composite material and an electrochemical enzyme sensor based on it. The conductive composite, consisting of poly(3,4-ethylenedioxy-thiophene-poly(4-lithium styrenesulfonic acid)/chitosan-AuNPs-glutaraldehyde, was created via electrochemical production. The sensor showed high catalytic activity towards the oxidation of polyphenols and could be used for their determination.
Article
Oncology
Halina Abramczyk, Jakub Maciej Surmacki
Summary: The multiple functions of cytochrome c go beyond respiration, apoptosis, ROS scavenging, and oxidation of cardiolipine. It is involved in the propagation of mitogenic signals and its redox status is regulated by retinoic acid. The study reveals the importance of retinoic acid in cellular processes and the role of cytochrome c in mitochondrial energy homeostasis.
Article
Chemistry, Multidisciplinary
Halina Abramczyk, Jakub Surmacki
Summary: This study investigates the impact of COVID-19 mRNA vaccine on human lung epithelial carcinoma cells using Raman spectroscopy and imaging. The results demonstrate that the vaccine reduces the concentration of cytochrome c, leading to a decrease in oxidative phosphorylation, reduced apoptosis, and decreased ATP production. Additionally, the vaccine increases lipid synthesis in lipid droplets and alters the biochemical composition temporarily.
Article
Multidisciplinary Sciences
Halina Abramczyk, Jakub Maciej Surmacki, Monika Kopec, Karolina Jarczewska, Beata Romanowska-Pietrasiak
Summary: This paper evaluates the oxidation and oxygenation effects in heme proteins using spectroscopy and imaging techniques. The study extends the current understanding of these effects and provides evidence for their strong coupling. The results also demonstrate the mechanism of electronic-vibrational coupling in the Q band splitting. The research has important implications for the role of heme proteins in maintaining biological functions.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Agnieszka Wosiak, Damian Wodzinski, Katarzyna Michalska, Jacek Pietrzak, Radzislaw Kordek, Ewa Balcerczak
Summary: This study aimed to investigate the association between the polymorphisms of SMAD3 and SMAD4 genes and the risk of colorectal cancer, but the results showed no significant correlation, indicating the need for further research to confirm the findings.
PHARMACOGENOMICS & PERSONALIZED MEDICINE
(2021)