Review
Clinical Neurology
Sheila Alcantara Llaguno, Luis F. Parada
Summary: Recent studies have revealed the complexity of glioma cancer stem cells (CSCs), including developmental origins, plasticity, cellular states, and interactions with the microenvironment. These cells exhibit diverse transcriptional cellular states, susceptible to transitions, playing important roles in treatment resistance and immune evasion.
CURRENT OPINION IN NEUROLOGY
(2021)
Review
Cell Biology
Maoyu Wang, Sen Shen, Feng Hou, Yaohua Yan
Summary: This article reviews the feasibility of treating gliomas by targeting integrins on glioma stem cells, elucidating the functions and mechanisms of integrins in glioma stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Marc-Antoine Da-Veiga, Bernard Rogister, Arnaud Lombard, Virginie Neirinckx, Caroline Piette
Summary: Pediatric high-grade glioma (pHGG) has a poor prognosis, especially in younger patients. The highly malignant glioma stem cells (GSCs) remain poorly investigated in pediatric cases, despite being well described in adult brain tumors. Understanding the role of GSCs in pediatric tumors could lead to targeted treatments for better outcomes.
Review
Cell Biology
Karina Biserova, Arvids Jakovlevs, Romans Uljanovs, Ilze Strumfa
Summary: Cancer stem cells, particularly in glioblastoma, play a crucial role in tumor resistance to treatment and recurrence. The regulatory mechanisms of glioma stem cells include tumor microenvironment, niche concept, metabolism, immunity, genetics, and epigenetics, which serve as potential targets for future treatments.Understanding the molecular markers and treatment resistance of GSCs in glioblastoma may lead to more effective therapeutic strategies in the future.
Article
Cell Biology
Sanchit Gandhi, Ashish Bhushan, Unmesh Shukla, Amit Pundir, Sanjeev Singh, Tapasya Srivastava
Summary: In this study, a newly developed tool called GenOx was used to identify hypoxia regulated long non-coding RNAs (lncRNAs) and discover Hypoxia Response Element (HRE) and Hypoxia Ancillary Sequence (HAS) motifs in their promoter regions. Molecular, functional, and interactome-based analyses showed that these hypoxia-relevant lncRNAs play important roles in primary tumors and cell-line models. TP53TG1 and SNHG1 were identified as highly relevant lncRNAs in glioma progression and downregulation of SNHG1 in hypoxia was associated with worsened prognosis.
Review
Biochemistry & Molecular Biology
Dominique Figarella-Branger, Carole Colin, Nathalie Baeza-Kallee, Aurelie Tchoghandjian
Summary: This review focuses on the expression of A2B5 in the central nervous system and gliomas. A2B5 is found in neural stem cells during brain development and in neurogenic areas and white matter in adults, where it identifies oligodendrocyte precursor cells and cells with neural stem cell properties. A2B5 has been identified as an accurate marker to identify glioma cancer stem cells and its expression is positively correlated with stemness and tumor growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Kouminin Kanwore, Konimpo Kanwore, Gabriel Komla Adzika, Ayanlaja Abdulrahman Abiola, Xiaoxiao Guo, Piniel Alphayo Kambey, Ying Xia, Dianshuai Gao
Summary: This review examines the underlying mechanisms of glioma initiation and metabolism activation, as well as the tumor microenvironment changes that affect epigenetic modifications in immune cells, from an oncological and immunological perspective. The potential for therapeutic intervention is also highlighted.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Sumei Xu, Haiyan Xiao, Tao Song, Youjie Zeng, Chenglong Wei, Tiange Chen, Zhongxu Hu, Ren Guo, Dai Li, Minghao Jiang
Summary: This study reveals the significant association between HOXD9 and the prognosis and survival of glioma patients. The upregulation of HOXD9 is correlated with malignant properties in glioma. High HOXD9 expression is indicative of a poor prognosis for glioma patients and is associated with reduced tumor purity and higher levels of immune invasion. These findings suggest the potential of HOXD9 as a prognostic biomarker and its role in influencing the glioma immune microenvironment.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Review
Neurosciences
Qiyuan Zhuang, Hui Yang, Ying Mao
Summary: Glioma, the most common and lethal primary brain tumor, is highly heterogeneous and its cell state, including glioma stem cells, microglia, neurons, and immune cells, play crucial roles in tumor initiation, progression, and response to therapy.
NEUROSCIENCE BULLETIN
(2023)
Review
Oncology
Soumaya Rafii, Sarah Kandoussi, Amina Ghouzlani, Oumayma Naji, Konala Priyanka Reddy, Rizwan Ullah Sadiqi, Abdallah Badou
Summary: Gliomas are highly malignant cancers with poor prognosis even after conventional therapies. The immunosuppressive microenvironment of gliomas hampers the anti-tumor immune response. Recent studies have shown promising results in reversing immune resistance and activating anti-tumor immune response through different immunotherapy strategies. Understanding the components and mechanisms of glioma's immune microenvironment is crucial for developing effective therapeutic pathways.
FRONTIERS IN ONCOLOGY
(2023)
Review
Oncology
A. B. Carpenter, A. M. Carpenter, R. Aiken, S. Hanft
Summary: Gliomas are challenging to treat due to their resistance to conventional therapies, but immunotherapy, particularly oncolytic viruses, has shown promising results in recent studies. The review provides a comprehensive overview of completed human clinical investigations on oncolytic viruses for glioma treatment.
ANNALS OF ONCOLOGY
(2021)
Article
Cell Biology
Jiajie Lu, Kairong Liang, Renheng Zou, Yuecheng Peng, Haojian Wang, Rihong Huang, Zhaorong Zeng, Zejia Feng, Yongyang Fan, Shizhen Zhang, Yunxiang Ji, Xiao Pang, Yezhong Wang, Hongri Zhang, Zhaotao Wang
Summary: This study evaluates the functions of 8 TRIM family members in gliomas using bioinformatics tools. The results show that except for TRIM17, the expression levels of the other 7 members are higher in gliomas than in normal tissues. High expression of TRIM5/21/22/24/28/34/47 is associated with poor prognosis in glioma patients. Furthermore, genetic alterations in the TRIM family are correlated with longer survival in glioma patients. Functional enrichment analysis suggests that these TRIM family members may affect glioma tumorigenesis by influencing immune infiltration and the expression of immune checkpoint molecules. Finally, a 6-gene signature predicts overall survival in glioma patients.
Article
Cell Biology
Ava Nasrolahi, Shirin Azizidoost, Klaudia Radoszkiewicz, Sajad Najafi, Farhoodeh Ghaedrahmati, Omid Anbiyaee, Seyed Esmaeil Khoshnam, Maryam Farzaneh, Shahab Uddin
Summary: Glioma is a common malignant brain tumor that arises from glial tissue. Glioma cancer stem cells (GCSCs) have been identified as playing important roles in tumor initiation and metastasis. Targeting GCSCs and their associated signaling pathways, as well as non-coding RNAs, could be a novel strategy for glioma treatment. In this review, critical signaling pathways involved in GCSC tumorigenesis and invasiveness are summarized.
CELLULAR SIGNALLING
(2023)
Article
Oncology
Ssu-Han Chen, Hong-Han Lin, Yao-Feng Li, Wen-Chiuan Tsai, Dueng-Yuan Hueng
Summary: High expression of TRIP13 in gliomas is associated with poor prognosis, potentially influencing tumor development by regulating co-expressed genes related to tumorigenesis and therapeutic resistance. Aberrant expression of TRIP13 may be regulated by mechanisms such as DNA methylation and miRNA targeting, leading to high aneuploidy levels and reduced CD8(+)/Treg ratio, which worsen patient outcomes.
Article
Immunology
Cheng Wei, Bo Wang, Dazhao Peng, Xiaoyang Zhang, Zesheng Li, Lin Luo, Yingjie He, Hao Liang, Xuezhi Du, Shenghui Li, Shu Zhang, Zhenyu Zhang, Lei Han, Jianning Zhang
Summary: Comprehensive bioinformatics analysis showed that ALKBH5 is upregulated in multiple solid tumors and promotes the development and progression of cancer through regulation of tumor immunity, as well as cell proliferation, migration, and infiltration.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Clinical Neurology
Daisuke Ito, Satoru Morimoto, Shinichi Takahashi, Kensuke Okada, Jin Nakahara, Hideyuki Okano
Summary: Significant progress has been made in drug screening for amyotrophic lateral sclerosis using patient-derived induced pluripotent stem cells, leading to the identification of candidate drugs and the advancement of clinical trials. This stem cell-based approach shows safety and positive effects, reducing costs and time compared to animal testing.
Article
Biotechnology & Applied Microbiology
Ryota Tamura, Hiroyuki Miyoshi, Kent Imaizumi, Masahiro Yo, Yoshitaka Kase, Tsukika Sato, Mizuto Sato, Yukina Morimoto, Oltea Sampetrean, Jun Kohyama, Munehisa Shinozaki, Atsushi Miyawaki, Kazunari Yoshida, Hideyuki Saya, Hideyuki Okano, Masahiro Toda
Summary: This study demonstrates the potential benefit of gene-edited induced pluripotent stem cells (iPSCs) for the treatment of invasive glioma stem cells (GSCs). Neural stem cells (NSCs) derived from CRISPR/Cas9-edited iPSCs expressing a suicide gene showed enhanced tumor-trophic migratory capacity and significant in vivo antitumor effects. The study also identified key signaling pathways and gene expressions associated with the efficacy of therapeutic iPSC-NSCs.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Dermatology
Shigeki Ohta, Aya Misawa, Chaw Kyi-Tha-Thu, Naomi Matsumoto, Yoshie Hirose, Yutaka Kawakami
Summary: Melanoma has been extensively studied as a model for cancer immunology research, and investigations into anti-tumor T-cell responses have provided valuable insights for immunotherapies. Individual differences in cancer-immune status are influenced by cancer cell characteristics, genetic backgrounds, and environmental and lifestyle factors. Recent studies using advanced technologies have led to advancements in human tumor immunology and the development of personalized immunotherapies for melanoma patients.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Cell Biology
Satoru Morimoto, Koichi Saeki, Masaru Takeshita, Kunio Hirano, Mariko Shirakawa, Yumiko Yamada, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano
Summary: The study developed an intranasal vaccine for COVID-19 by using a Sendai virus vector loaded with the receptor binding domain of SARS-CoV-2 as an antigen. The vaccine induced a strong immune response, with increased levels of S-RBD-specific antibodies in serum and bronchoalveolar lavage fluid. The booster dose further enhanced the production of neutralizing antibodies, indicating the effectiveness of the vaccine.
Article
Cell Biology
Sho Yoshimatsu, Mayutaka Nakajima, Iki Sonn, Rie Natsume, Kenji Sakimura, Ena Nakatsukasa, Toshikuni Sasaoka, Mari Nakamura, Takashi Serizawa, Tsukika Sato, Erika Sasaki, Hongkui Deng, Hideyuki Okano
Summary: This study compared the gene expression profiles of mouse, human, and common marmoset EPSCs using transcriptomic approaches. The marmoset EPSC-like cells displayed a distinct morphology but exhibited a pluripotent state similar to embryonic stem cells. They also showed interspecies chimeric contribution to mouse embryos. Furthermore, the gene expression of the marmoset EPSC-like cells resembled that of human EPSCs. Overall, this study evaluated the efficacy of the method for deriving marmoset EPSCs.
Article
Biochemistry & Molecular Biology
Tosho Kondo, Ihori Ebinuma, Hirotaka Tanaka, Yukitoshi Nishikawa, Takaki Komiya, Mitsuru Ishikawa, Hideyuki Okano
Summary: In this study, an effective high-throughput screening system was established using induced pluripotent stem cells (iPSCs) to generate motor neurons. The generated motor neurons exhibited characteristics similar to ALS neurons and showed abnormal protein accumulation and hyperexcitability. Treatment with rapamycin and retigabine normalized activity and improved neuronal survival. This study provides a valuable platform for discovering novel ALS therapeutics and personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Shota Okura, Honoka Ishii, Ayano Suzuki, Chika Saegusa, Ko Fujiki, Kenshi Sugano, Noriomi Suzuki, Tsubasa Saeki, Saeko Matsuzaki, Hiroyuki Ozawa, Masato Fujioka, Makoto Hosoya, Hideyuki Okano
Summary: We have established two human-induced pluripotent stem cell (iPSC) lines from individuals without auditory disorders and confirmed normal hearing through extensive audiometry tests. These iPSC lines express pluripotency genes and are capable of differentiating into the three germ layers. They will be used as controls for pathological analysis and drug screening for ear disorders.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ryotaro Imai, Ryota Tamura, Masahiro Yo, Mizuto Sato, Mariko Fukumura, Kento Takahara, Yoshitaka Kase, Hideyuki Okano, Masahiro Toda
Summary: Despite limited treatment options for traumatic brain injury (TBI), neural stem/progenitor cells (NS/PCs) derived from induced pluripotent stem cells (iPSCs) expressing yCD-UPRT showed promising results in functional recovery. The transplanted iPSC-derived NS/PCs displayed stable expression of the therapeutic gene and concentrated around the damaged cortex, leading to improved behavioral performance and prevention of secondary brain injury in a TBI mouse model. In addition, selective elimination of undifferentiated NS/PCs using 5-fluorocytosine (5-FC) demonstrated the safety of this regenerative medicine approach.
Article
Multidisciplinary Sciences
Cecile Thirant, Agathe Peltier, Simon Durand, Amira Kramdi, Caroline Louis-Brennetot, Cecile Pierre-Eugene, Margot Gautier, Ana Costa, Amandine Grelier, Sakina Zaidi, Nadege Gruel, Irene Jimenez, Eve Lapouble, Gaelle Pierron, Deborah Sitbon, Herve J. Brisse, Arnaud Gauthier, Paul Freneaux, Sandrine Grossetete, Laura G. Baudrin, Virginie Raynal, Sylvain Baulande, Angela Bellini, Jaydutt Bhalshankar, Angel M. Carcaboso, Birgit Geoerger, Hermann Rohrer, Didier Surdez, Valentina Boeva, Gudrun Schleiermacher, Olivier Delattre, Isabelle Janoueix-Lerosey
Summary: Noradrenergic and mesenchymal identities have been characterized in neuroblastoma cell lines. However, their relationship and contribution in patient tumors remain unclear. This study documents spontaneous and reversible plasticity between the two identities, associated with epigenetic reprogramming. The microenvironment plays a role in driving cell identity, as xenografts eventually harbor a noradrenergic phenotype.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Miho Isoda, Tsukasa Sanosaka, Ryo Tomooka, Yo Mabuchi, Munehisa Shinozaki, Tomoko Andoh-Noda, Satoe Banno, Noriko Mizota, Ryo Yamaguchi, Hideyuki Okano, Jun Kohyama
Summary: A single-cell approach identified cellular properties of neural and mesenchymal origins in human induced pluripotent stem cells, shedding light on the causes of undesired grafts after transplantation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hitomi Ono-Minagi, Tsutomu Nohno, Takashi Serizawa, Yu Usami, Takayoshi Sakai, Hideyuki Okano, Hideyo Ohuchi
Summary: This study investigates the embryonic origin of salivary and lacrimal glands using transgenic mice and demonstrates that these glands contain cells derived from all three germ layers. Specifically, a subset of cells derived from neural crest differentiate into epithelial cells and express high levels of stem cell markers. These cells play an important role in the regenerative response following tissue damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Hiroya Kobayashi, Koji Ueda, Satoru Morimoto, Mitsuru Ishikawa, Nicolas Leventoux, Ryogen Sasaki, Yoshifumi Hirokawa, Yasumasa Kokubo, Hideyuki Okano
Summary: The proteins in extracellular vesicles derived from astrocytes in patients with Kii ALS/PDC exhibit distinctive patterns compared to healthy individuals, with increased proteins related to proteostasis and decreased proteins related to anti-inflammation.
NEUROLOGICAL SCIENCES
(2023)
Review
Medicine, General & Internal
Takahiro Shibata, Syoichi Tashiro, Masaya Nakamura, Hideyuki Okano, Narihito Nagoshi
Summary: Cell transplantation therapy using human-induced pluripotent stem cell-derived neural stem/progenitor cells has shown efficacy in treating the subacute phase of spinal cord injury. However, there is a lack of focus on treatment in the chronic phase, which poses challenges in overcoming factors specific to chronic SCI. Combining different therapies and addressing issues in severe injury models and functional recovery are important for regenerative therapy in chronic SCI.
MEDICINA-LITHUANIA
(2023)
Article
Geriatrics & Gerontology
Shohei Komaki, Masatoshi Nagata, Eri Arai, Ryo Otomo, Kanako Ono, Yukiko Abe, Hideki Ohmomo, So Umekage, Natsuko Shinozaki, Tsuyoshi Hachiya, Yoichi Sutoh, Yayoi Otsuka-Yamasaki, Yasumichi Arai, Nobuyoshi Hirose, Akio Yoneyama, Hideyuki Okano, Makoto Sasaki, Yae Kanai, Atsushi Shimizu
Summary: This study explored the epigenetic signatures of exceptional healthy longevity and found that it depends not only on maintaining young epigenetic states but also on advanced states of specific epigenetic regions.
LANCET HEALTHY LONGEVITY
(2023)
Review
Biochemistry & Molecular Biology
Hideyuki Okano, Satoru Morimoto, Chris Kato, Jin Nakahara, Shinichi Takahashi
Summary: In recent years, clinical trials using iPSC technology for disease modeling and drug discovery have shown promising results, particularly for the treatment of ALS. ROPi has been identified as a potential ALS treatment and has undergone clinical trials and reverse translational research, demonstrating its ability to delay disease progression and prolong survival in ALS patients.
JOURNAL OF NEUROCHEMISTRY
(2023)
