Article
Multidisciplinary Sciences
Jinkwan Jo, Min-Young Kang, Kyung Seok Kim, Hye Rim Youk, Eun-Jo Shim, Hongsup Kim, Jee-Soo Park, Sung-Chur Sim, Byung Cheon Yu, Jin-Kee Jung
Summary: In this study, improvements were made to the investigation method for plant variety protection by utilizing core single nucleotide polymorphism markers in a high-throughput genotyping system. The results showed that the core marker sets could effectively identify different cabbage varieties and traits, and they were used in practical applications in related fields.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Meriem Wafaa Khalifa, Noureddine Rouag, Mariem Bouhadida
Summary: This study isolated Pseudomonas rhizobacteria with plant growth-promoting properties to control chickpea wilt disease caused by Fusarium oxysporum f. sp. ciceris. The isolated rhizobacteria produced antifungal compounds and caused morphological changes in Foc. They also produced various plant-growth-promoting compounds and significantly increased chickpea growth and reduced wilt disease.
Article
Genetics & Heredity
Long Tong, Cunbao Zhao, Jinhui Liu, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Jialei Ji, Bifeng Kuang, Kelan Tang, Zhiyuan Fang, Ryo Fujimoto, Honghao Lv
Summary: Cabbage Fusarium wilt is a major threat to cabbage production. This study found that some cabbage lines have resistance to both races of the pathogen, while others are susceptible to race 2. Genetic analysis revealed that resistance to race 2 is controlled by multiple genes. These findings have implications for breeding and gene mining for resistance to Fusarium wilt in cabbage.
Article
Biology
Keziban Yazici, Betul Goenuelkirmaz, Mehtap Sahin Cevik
Summary: In this study, molecular markers associated with seed hardness in pomegranates were developed to distinguish soft-seeded cultivars. These markers enable quick and accurate identification of soft-seeded pomegranate varieties, meeting the market demand.
Article
Agronomy
Zhiliang Xiao, Congcong Kong, Fengqing Han, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Jialei Ji, Zhansheng Li, Zhiyuan Fang, Honghao Lv
Summary: By resequencing cabbage lines, researchers were able to develop accurate markers MBoHL1 and KBoHL2 for rapid identification of HL genes in cabbage. These markers showed 100% accuracy in eight inbred lines and play an important role in preventing genetic death during breeding processes.
Article
Plant Sciences
Naomi Miyaji, Mst Arjina Akter, Chizuko Suzukamo, Hasan Mehraj, Tomoe Shindo, Takeru Itabashi, Keiichi Okazaki, Motoki Shimizu, Makoto Kaji, Masahiko Katsumata, Elizabeth S. Dennis, Ryo Fujimoto
Summary: Fusarium yellows in Brassica rapa L. is caused by Fusarium oxysporum f. sp. rapae or F. oxysporum f. sp. conglutinans. While a resistance gene against F. oxysporum f. sp. conglutinans has been identified, a resistance gene against F. oxysporum f. sp. rapae has not been found. Inoculation tests showed cross-resistance between the two pathogens in resistant and susceptible lines. However, disease resistance prediction based on a DNA marker was not always accurate. QTL-seq analysis identified a specific locus on chromosome A03 related to disease resistance. Amino acid sequence comparisons revealed differences between susceptible and resistant alleles, with the presence of FocBr1 gene essential for resistance to F. oxysporum f. sp. rapae.
Article
Microbiology
Huacai Fan, Ping He, Shengtao Xu, Shu Li, Yongfen Wang, Wenlong Zhang, Xundong Li, Hui Shang, Li Zeng, Si-Jun Zheng
Summary: By sequencing the soil microbial diversity in six main banana-producing areas in Yunnan, this study found that Chujaibacter, Bacillus, and Sphingomonas were significantly enriched. Further correlation analysis revealed a significant negative correlation between Bacillus and the soil pathogen Foc TR4. Thus, a B. velezensis strain YN1910 was isolated from disease-suppressive soils, which showed significant control (78.43-81.76%) of banana Fusarium wilt and significant growth promotion on banana plants in vitro and pot experiments.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Raman Thangavelu, Esack Edwinraj, Muthukathan Gopi, Periyasamy Pushpakanth, Kotteswaran Sharmila, Manivasakan Prabaharan, Murugan Loganathan, Subbaraya Uma
Summary: This study identified the genomic differences and effector gene profiles of Indian Foc isolates, and developed specific molecular markers for Indian Foc R1, R4, and TR4. These markers can be used for accurate diagnosis and detection of the pathogen.
Article
Mycology
Loan Le Thi, Arne Mertens, Dang Toan Vu, Tuong Dang Vu, Pham Le Anh Minh, Huy Nguyen Duc, Sander de Backer, Rony Swennen, Filip Vandelook, Bart Panis, Mario Amalfi, Cony Decock, Sofia I. F. Gomes, Vincent S. F. T. Merckx, Steven B. Janssens
Summary: Fusarium oxysporum f. sp. cubense is an important plant pathogenic fungus that causes losses in banana cultivation, with predictions showing increasing impact in Vietnam. This study identified and assessed the different Foc isolates in northern Vietnam using a combined morphological-molecular approach, and estimated the proportion of different Fusarium races infecting bananas.
Review
Forestry
Dario Grattapaglia
Summary: GS has rapidly evolved from a hyped topic to a leading area of applied research in tree breeding, surpassing the initial phases of the hype cycle and moving towards wide application. By integrating modern DNA typing, quantitative genetics, and mixed-model analysis, GS has revolutionized the breeding approach by predicting complex phenotypes through genomic effects, leading to enhanced genetic gain and breeding value accuracy in forest trees.
Review
Plant Sciences
Xiaoyi Wang, Renbo Yu, Jingyang Li
Summary: Bananas are an important fruit crop threatened by the fungus Fusarium oxysporum f. sp. cubense (Foc) which causes Fusarium wilt. Functional genetic studies of the Musa genome and genome editing experiments can lead to the development of banana varieties with both Foc resistance and ideal plant architecture.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Bimala Pant, Tingting Bai, Chanjuan Du, Suraj Baidya, Prem Bahadur Magar, Shrinkhala Manandhar, Jiban Shrestha, Miguel Dita, Mathieu Rouard, Gang Fu, Si-Jun Zheng
Summary: Fusarium wilt of banana caused by F. oxysporum f. sp. cubense was observed in Malbhog cultivar in Nepal. Thirteen strains of F. oxysporum causing FWB symptoms were identified. The study reported the occurrence of FWB in Nepal for the first time.
Review
Biotechnology & Applied Microbiology
S. Nakkeeran, S. Rajamanickam, R. Saravanan, M. Vanthana, K. Soorianathasundaram
Summary: Banana, a major cash crop worldwide, is threatened by Fusarium wilt; introducing endophytes from resistant genotypes into banana plants can enhance immunity against Fusarium and ongoing research focuses on exploring the potential of these endophytes to develop effective control methods.
Review
Agronomy
Liqiang Song, Ruihui Wang, Xueju Yang, Aimin Zhang, Dongcheng Liu
Summary: Wheat, an essential cereal crop, provides a significant amount of calories and proteins consumed by humans. Wheat breeders face challenges in developing new varieties to meet production requirements. Marker-assisted selection (MAS) has advantages in terms of time-saving, cost-effectiveness, and goal orientation. This review provides updated information on different molecular markers and their potential applications in wheat breeding programs.
Review
Biochemistry & Molecular Biology
Gloria De Mori, Guido Cipriani
Summary: Breeding fruit trees is challenging due to their large trees, long juvenile periods, and environmental variability. The use of molecular markers to assist breeders in selecting genetically superior parents and progeny has been a difficult task. Important fruit traits such as size, weight, sugar and acid content, and ripening time are of interest to fruit breeders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Kang Zhang, Yinqing Yang, Jian Wu, Jianli Liang, Shumin Chen, Lei Zhang, Honghao Lv, Xiaona Yin, Xin Zhang, Yiyue Zhang, Lingkui Zhang, Yangyong Zhang, Michael Freeling, Xiaowu Wang, Feng Cheng
Summary: Leaf heading is an important horticultural trait in vegetables. This study identifies the transcriptional regulation mechanism and developmental switch involved in the transition to leaf heading in Chinese cabbage and cabbage. The launch of the transition stage is closely associated with ambient temperature, and the ethylene pathway is hypothesized to have been targeted for selection during the domestication of Chinese cabbage to form the heading trait. These findings provide new insights for future studies of leafy head crops.
Review
Biochemistry & Molecular Biology
Jialei Ji, Limei Yang, Zhiyuan Fang, Yangyong Zhang, Mu Zhuang, Honghao Lv, Yong Wang
Summary: This review focuses on the advances of the plant SWEETs, including their discovery, protein structure characteristics, evolution, physiological functions, and applications in plant breeding. It provides more in-depth and comprehensive information to elucidate the molecular basis of SWEETs' function in plants.
Article
Biochemistry & Molecular Biology
Congcong Kong, Henan Su, Siping Deng, Jialei Ji, Yong Wang, Yangyong Zhang, Limei Yang, Zhiyuan Fang, Honghao Lv
Summary: In this study, the regulatory roles of DNA methylation and miRNA in early microspore embryogenesis (ME) were investigated in cabbage. The results showed that heat shock induced DNA methylation and miRNA, which influenced ME by modulating reactive oxygen species (ROS) response and abscisic acid (ABA) signaling. These findings provide new insights into the molecular regulation of ME induced by heat shock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Wendi Chen, Bin Zhang, Wenjing Ren, Li Chen, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Yangyong Zhang
Summary: In this study, the self-compatibility of 58 winter cabbage inbred lines was identified using open-flower self-pollination and molecular techniques. A new S-haplotype-specific marker was developed and 9 distinct S-haplotypes were found in the cabbage inbred lines. A Tri-Primer-PCR amplification method was established for rapid selection of plants with specific S-haplotypes. The study also overcame parental cross-incompatibility by selecting an inbred line with a specific S-haplotype.
Review
Biochemistry & Molecular Biology
Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
Summary: Hybrid production using Ogura CMS has become an important approach to harness heterosis in vegetables. This article provides an overview of the mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops, and discusses the current challenges and future research directions in the application of Ogura CMS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bin Zhang, Jiao Wang, Li Chen, Wenjing Ren, Fengqing Han, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Yangyong Zhang
Summary: This study investigates the molecular regulatory mechanism of petal color formation in cabbage and identifies key genes involved in carotenoid biosynthesis in petals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Li Chen, Wenjing Ren, Bin Zhang, Huiling Guo, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Xilin Hou, Yangyong Zhang
Summary: In this study on cabbage, the cytology and transcriptome analysis were performed to investigate the microspore development process and gene expression changes in Ogura cytoplasmic male sterility (CMS) lines. The results showed that early degradation of tapetal cells in Ogura CMS caused pollen abortion. Transcriptome analysis revealed the dysregulated expression of genes related to energy metabolism in Ogura CMS, leading to decreased ATP content and excessive ROS accumulation in anthers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Peng Wang, Wenxue Cao, Limei Yang, Yangyong Zhang, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Shanhan Cheng, Jialei Ji
Summary: The synthesis of glucosinolates (GSLs) in cabbage was studied by analyzing the cabbage genome and identifying 193 GSLs biosynthetic genes (GBGs). It was found that the expression levels of these GBGs were significantly altered by different exogenous hormones. This study provides a foundation for regulating GSLs synthesis in cabbage through gene editing and overexpression.
Article
Plant Sciences
Kaiwen Yuan, Xinyu Zhao, Wenru Sun, Limei Yang, Yangyong Zhang, Yong Wang, Jialei Ji, Fengqing Han, Zhiyuan Fang, Honghao Lv
Summary: This study identified the gene BoNA1, which is responsible for the absence of anthocyanins in curly kale. The function of BoNA1 was confirmed through gene editing technology, improving our understanding of the regulation mechanism of anthocyanin accumulation in Brassica oleracea subspecies.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Jiao Wang, Bin Zhang, Huiling Guo, Li Chen, Fengqing Han, Chao Yan, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Yangyong Zhang
Summary: In this study, the molecular regulatory mechanism underlying flowering time regulation in cabbage was investigated using transcriptome analysis. Two candidate genes, BoSEP2-1 and BoSEP2-2, were identified. These findings provide new insights into the molecular mechanisms underlying flowering time regulation in cabbage.
Article
Microbiology
Yuankang Wu, Bin Zhang, Shaobo Liu, Zhiwei Zhao, Wenjing Ren, Li Chen, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Fengqing Han, Yangyong Zhang
Summary: This study presents the first whole-genome sequence of Hyaloperonospora parasitica isolate BJ2020, a pathogen that causes downy mildew in cabbage. The genome contains 4631 contigs and 9991 protein-coding genes, with a size of 37.10 Mb. Comparative analysis revealed the close phylogenetic affinity between H. parasitica and H. brassicae, providing important insights into the pathogenic mechanisms of H. parasitica BJ2020.
Article
Agronomy
Longxiang Yan, Zhiyuan Fang, Na Zhang, Limei Yang, Yangyong Zhang, Mu Zhuang, Honghao Lv, Jialei Ji, Yong Wang
Summary: This study examined 10 BoGGPPS genes from B. oleracea and found that they are mainly expressed in chloroplasts and plastids. Similar BoGGPPS genes have similar structure and motifs. Distribution, collinearity, and Ka/Ks analysis revealed multiple duplications in the BoGGPPS gene family. Cis-acting element analysis suggests that cabbage BoGGPPS genes may participate in light and hormone responses. Three-dimensional structure analysis shows the abundance of α-helices and random coils among BoGGPPS members, indicating their important functions. The findings provide a basis for further exploration and functional analysis of BoGGPPS genes and their relationship with terpenoids in chlorophyll synthesis in B. oleracea.
Article
Horticulture
Dan Wu, Miao Ni, Xin Lei, Liping Zhang, Wei Zhang, Huangying Shu, Zhiwei Wang, Jie Zhu, Shanhan Cheng, Pingwu Liu, Honghao Lv, Limei Yang
Summary: This study analyzed the genome-wide CCR gene family in pepper and identified 38 CCR genes, and characterized the enzymic kinetic parameters and regulatory function of two of them. The study found that CCRs have an impact on capsaicin content, with CCR2 playing a more important role in regulating pungency formation.
Article
Plant Sciences
Mingzhao Zhu, Yong Wang, Shujin Lu, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Zhiyuan Fang, Xilin Hou
Summary: In this study, a total of 36 CKX genes were identified in cabbage using a genome-wide search method. Phylogenetic analysis classified these genes into three groups. These genes were unevenly distributed across nine chromosomes in B. oleracea, with 15 genes not containing any introns. The expression patterns of these genes in various tissues and organs were shown through a heatmap. Three genes were found to be relatively highly expressed in all investigated tissues under normal conditions, while three other genes showed significant down-regulation and up-regulation in susceptible and resistant materials after inoculation, indicating their potential roles in resistance to clubroot disease.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Plant Sciences
Mingzhao Zhu, Limei Yang, Yangyong Zhang, Mu Zhuang, Jialei Ji, Xilin Hou, Zhansheng Li, Fengqing Han, Zhiyuan Fang, Honghao Lv, Yong Wang
Summary: In this study, clubroot resistance (CR) genes from Brassica rapa were introduced into the cabbage genome, making it resistant to clubroot disease. The mechanism of gene introgression was explored through cytoplasmic replacement and distant hybridization, revealing homoeologous exchange as the basis for the introgression of CR resistance. The successful introduction of CR into the cabbage genome provides valuable insights for creating introgression lines in other species of interest.
HORTICULTURE RESEARCH
(2022)
