期刊
MOLECULAR BREEDING
卷 30, 期 4, 页码 1799-1809出版社
SPRINGER
DOI: 10.1007/s11032-012-9745-4
关键词
Vicia faba L.; KBiosciences competitive allele-specific PCR (KASP); Single nucleotide polymorphism (SNP); Haplotype
资金
- Defra as part of the Pulse Crop Genetic Improvement Network II (PCGINII) project
The aim of this study was to convert existing faba bean (Vicia faba L.) single nucleotide polymorphism (SNP) markers from cleaved amplification polymorphic sequences and SNaPshot(A (R)) formats, which are expensive and time-consuming, to the more convenient KBiosciences competitive alleleaEurospecific PCR (KASP) assay format. Out of 80 assays designed, 75 were validated, though a core set of 67 of the most robust markers is recommended for further use. The 67 best KASP SNP assays were used across two generations of single seed descent to detect unintended outcrossing and to track and quantify loss of heterozygosity, a capability that will significantly increase the efficiency and performance of pure line production and maintenance. This same set of assays was also used to examine genetic relationships between the 67 members of the partly inbred panel, and should prove useful for line identification and diversity studies in the future.
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