4.6 Article

Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)

期刊

MOLECULAR BREEDING
卷 27, 期 1, 页码 119-128

出版社

SPRINGER
DOI: 10.1007/s11032-010-9511-4

关键词

SSR markers; Enriched library; Cultivar identification

向作者/读者索取更多资源

The pomegranate (Punica granatum L.) is a temperate climate species requiring high temperatures for proper and complete ripening. The species is consumed as a fresh fruit, but also can be used to obtain transformed products such as juice, jam, or preserve. It is a fruit tree species with a high degree of diversity, but the identification of cultivars by morphological traits is very difficult. Thus, the characterization of genotypes through molecular markers is of great value for germplasm preservation, genetic studies, and plant breeding. The number of simple sequence repeat (SSR or microsatellite) markers developed for this genus is too low, so in this work we report the development of 117 microsatellite loci from a CT/AG-enriched pomegranate genomic library. In order to check their utility, eleven accessions were analyzed. The polymorphism information content (PIC) value across all loci ranged between 0.09 and 0.71, with an average of 0.37. These markers will facilitate genetic diversity studies, mapping, and genotyping of pomegranate.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Plant Sciences

Unravelling consensus genomic regions conferring leaf rust resistance in wheat via meta-QTL analysis

Aduragbemi Amo, Jose Miguel Soriano

Summary: Leaf rust resistance in wheat was studied by collecting 393 QTL from 50 mapping studies and identifying 15 high-confidence MQTL. This research provides valuable insights for developing resistant wheat cultivars.

PLANT GENOME (2022)

Correction Agronomy

Polo-Oltra et al. Cost-Effective and Time-Efficient Molecular Assisted Selection for PPV Resistance in Apricot Based on ParPMC2 Allele-Specific PCR (vol 10, 1292, 2020)

Angela Polo-Oltra, Carlos Romero, Inmaculada Lopez, Maria Luisa Badenes, Elena Zuriaga

AGRONOMY-BASEL (2022)

Article Plant Sciences

Characterization of the Spanish Pomegranate Germplasm Collection Maintained at the Agricultural Experiment Station of Elche to Identify Promising Breeding Materials

Elena Zuriaga, Jitka Pintova, Julian Bartual, Maria Luisa Badenes

Summary: This study analyzed the genetic and morphological characteristics of a pomegranate germplasm collection. The results identified various genetic profiles that can be used for varietal identification and breeding. Further genetic analysis can help select suitable parental materials for hybridization and use molecular assisted selection to obtain breeding offspring.

PLANTS-BASEL (2022)

Article Genetics & Heredity

A consensus map for quality traits in durum wheat based on genome-wide association studies and detection of ortho-meta QTL across cereal species

Ilaria Marcotuli, Jose Miguel Soriano, Agata Gadaleta

Summary: This study focused on identifying QTL hotspots in durum wheat for various quality traits using a collection of GWAS studies. Through the analysis of MTA on different chromosomes, a total of 94 QTL hotspots were identified, with candidate genes associated with biological processes controlling quality traits.

FRONTIERS IN GENETICS (2022)

Article Plant Sciences

Uncovering the genetic basis for quality traits in the Mediterranean old wheat germplasm and phenotypic and genomic prediction assessment by cross-validation test

Venkata Rami Reddy Yannam, Marta Lopes, Carlos Guzman, Jose Miguel Soriano

Summary: This study conducted a genome-wide association analysis using 170 landraces from 24 Mediterranean countries, and identified QTL hotspots related to wheat quality and agronomic traits, as well as candidate genes. The accuracies of genomic prediction were calculated using cross-validation, and five prediction equations were developed to predict bread loaf volume.

FRONTIERS IN PLANT SCIENCE (2023)

Editorial Material Biochemistry & Molecular Biology

New Breeding Technologies in Grasses

Agata Gadaleta, Jose Miguel Soriano

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Plant Sciences

Improving in-season wheat yield prediction using remote sensing and additional agronomic traits as predictors

Adrian Gracia-Romero, Ruben Rufo, David Gomez-Candon, Jose Miguel Soriano, Joaquim Bellvert, Venkata Rami Reddy Yannam, Davide Gulino, Marta S. Lopes

Summary: The development of accurate multivariate models for grain yield (GY) prediction in wheat experimental trials using normalized difference vegetation index (NDVI) and additional agronomic traits is a promising option to replace laborious in-field evaluations. This study proposed improved GY prediction models by incorporating NDVI, plant height, phenology, and ear density from experimental trials. The best models included NDVI, days to heading, and ear density or plant height, which showed a significant increase in prediction accuracy and a decrease in root mean square error.

FRONTIERS IN PLANT SCIENCE (2023)

Article Plant Sciences

Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness

Patricio Olmedo, Baltasar Zepeda, Joaquin Delgado-Rioseco, Carol Leiva, Adrian A. Moreno, Karen Sagredo, Francisca Blanco-Herrera, Romina Pedreschi, Rodrigo Infante, Claudio Meneses, Reinaldo Campos-Vargas

Summary: This study compared the changes in metabolite abundance in two nectarine varieties (Andes Nec-2 and Andes Nec-3) with different susceptibility to mealiness during cold storage using GC-MS analysis. The results showed that in unripe nectarines, cold storage led to an increase in the accumulation of amino acids, whereas in ripe nectarines, it resulted in a decrease in amino acid accumulation and an increase in the abundance of sugars and organic acids. Pathway reconstruction revealed that cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle in ripe nectarines, leading to differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines.

PLANTS-BASEL (2023)

Article Biology

COVID-19 in Italy: Is the Mortality Analysis a Way to Estimate How the Epidemic Lasts?

Pietro M. Boselli, Jose M. Soriano

Summary: Every epidemic brings health, economic, social, and environmental problems that need to be addressed promptly. This study uses a mathematical model to analyze the trends of infected and deceased individuals, aiming to predict the duration and phases of the epidemic. The analysis of cumulative deaths provides more accurate forecasts of the epidemic's development.

BIOLOGY-BASEL (2023)

Article Plant Sciences

Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.)

Tania Dorta, Francisco Gil-Munoz, Fany Carrasco, Elena Zuriaga, Gabino Rios, Manuel Blasco

Summary: This study investigated the involvement of effectors and transcriptional regulators in persimmon fruit maturation by analyzing the expression of genes involved in ethylene biosynthesis and response pathways. Results showed different gene expression patterns during fruit development, with some genes up-regulated and some genes down-regulated. The study also identified molecular mechanisms for high ethylene production in immature fruits and for fruit softening and skin colour change during natural ripening.

PLANTS-BASEL (2023)

Article Plant Sciences

Single-Bud Expression Analysis of Bud Dormancy Factors in Peach

Ana Puertes, Helin Polat, Luis Andres Ramon-Nunez, Matilde Gonzalez, Gema Ancillo, Elena Zuriaga, Gabino Rios

Summary: Transcriptomic and gene expression analysis help identify and characterize transcriptional regulatory factors and effectors in bud development. Single-bud gene analysis in peach flower buds reveals the variability in gene expression during dormancy release, contributing to the understanding of co-expression modules and regulatory aspects in this tissue.

PLANTS-BASEL (2023)

Proceedings Paper Agricultural Engineering

The Chilean nectarine breeding program toward development of chilling injury-resistant cultivars

R. Infante

Summary: Chile has a long-standing tradition of exporting nectarines, but faced challenges with maintaining quality during long shipping periods. In response, a breeding program was initiated to develop cultivars suitable for extended postharvest periods.

X INTERNATIONAL PEACH SYMPOSIUM (2022)

Article Horticulture

Transcriptomic Analysis of Sex-Associated DEGs in Female and Male Flowers of Kiwifruit (Actinidia deliciosa [A. Chev] C. F. Liang & A. R. Ferguson)

Patricio Zapata, Makarena Gonzalez, Igor Pacheco, Claudia Jorquera, Claudia Silva-Andrade, Marco Isaac Garrido, Rodrigo Infante, Juan Alfonso Salazar

Summary: This study analyzed the molecular mechanisms involved in sexual differentiation in kiwifruit and identified key genes and pathways related to hormonal signaling pathways, development of floral organs, and biological and metabolic processes. The findings provide insights into the sex determination of kiwifruit and the development of flower organs.

HORTICULTURAE (2022)

暂无数据