Article
Horticulture
Cara Still, Jacob Snelling, Shawn Mehlenbacher, Ryan Contreras
Summary: This study evaluated the use of SSR markers developed in Buddleja for cultivar identification. The markers Bud_03, Bud_10, and Bud_13 were found to be polymorphic and capable of accurately identifying cultivars. These markers may be useful for breeders in protecting their intellectual property and for identifying mislabeled cultivars.
Article
Horticulture
Xing Li, Bin Zheng, Wentian Xu, Xiaowei Ma, Songbiao Wang, Minjie Qian, Hongxia Wu
Summary: This study aimed to establish a rapid and accurate molecular detection method to identify the authenticity of hybrids in mango breeding. By using SSR primers and capillary electrophoresis, 62 true hybrids were successfully identified, and different SSR loci showed different genetic segregation patterns. Cluster analysis results showed that the parents and hybrids could be classified into two categories, which is helpful for mango variety protection and genetic research.
Article
Food Science & Technology
Talucci Giulia, Giulia Vallauri, Vera Pavese, Nadia Valentini, Paola Ruffa, Roberto Botta, Daniela Torello Marinoni
Summary: Establishing an efficient traceability system is crucial for avoiding fraudulent practices in hazelnut products. This study successfully identified a specific hazelnut cultivar through testing the efficiency of SSR markers.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Plant Sciences
Jian Zhang, Jingjing Yang, Yanling Lv, Xiaofei Zhang, Changxuan Xia, Hong Zhao, Changlong Wen
Summary: By constructing DNA fingerprints and analyzing the genetic structure of 259 melon varieties, it was found that Chinese melon varieties can be classified into five subgroups. The ssp. agrestis subgroup has a narrow genetic background and requires increased gene exchange with the ssp. melo subgroup. Core sets of markers were selected for efficient application in variety identification, monitoring, and intellectual property rights protection.
Article
Ecology
Haixia Zhong, Fuchun Zhang, Xiaoming Zhou, Mingqi Pan, Juan Xu, Jingzhe Hao, Shouan Han, Chuang Mei, He Xian, Min Wang, Jiahui Ji, Wei Shi, Xinyu Wu
Summary: The study utilized whole-genome sequencing to reveal the genetic information of the Munake grape cultivar, including millions of mutation markers and thousands of tags. The research validated some of the markers and delved deeper into the genetic makeup of the Munake cultivar.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Jingdian Liu, Xiyong Wang, Ting Lu, Jiancheng Wang, Wei Shi
Summary: Ammopiptanthus is a relict species with ancestral traits, surviving from the ancient Mediterranean retreat and climate drought. It is the only genus of super xerophytic, evergreen, broad-leaved shrubs. The genetic diversity study of Ammopiptanthus nanus, a species predominantly found in extremely arid and frigid environments, is crucial for its survival and ex situ conservation efforts.
Article
Agronomy
Jordana Caroline Nagel, Tales Poletto, Marlove Fatima Briao Muniz, Igor Poletto, Joana Nascimento Merces de Oliveira, Valdir Marcos Stefenon
Summary: This study used plastid SSR markers to identify pecan cultivars in southern Brazil and found possible misassignments among some cultivars. It also revealed the parental relationship and genetic diversity among certain cultivars. The plastid microsatellite markers used in this study are important tools for advancing pecan breeding programs.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Agronomy
Weiqing Dong, Fanglian He, Shaolong Wei, Zuyang Qiu, Qi Chen
Summary: A set of SSR markers was developed in this study to aid research on taro germplasm management. A total of 4438 SSRs were identified using RAD sequencing, with 3919 primer pairs designed based on the SSR data. Trinucleotide repeats were the most abundant type of SSR, and AT was the most common motif type. Through PCR amplification efficiency and phylogenetic tree analysis, the SSR markers identified in this study provide a useful resource for taro genetic and germplasm research.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Xinggu Lin, Shiou Yih Lee, Jianzhong Ni, Xiaomin Zhang, Xing Hu, Peishan Zou, Wei Wang, Guofeng Liu
Summary: This study sequenced and assembled the chloroplast genomes of Bougainvillea spectabilis 'Splendens' and compared it with other Bougainvillea species. The results provide valuable genetic resources for Bougainvillea breeding and offer molecular markers for species identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ambika, Venkatraman Hegde, M. S. Nimmy, C. Bharadwaj, Shailesh Tripathi, Rajesh Kumar Singh, Rajendra Kumar
Summary: Chickpea plants exhibit genetic and molecular marker differences between indeterminate and semi-determinate types, with early SSR markers proving effective in selecting different stem growth habits in chickpea plants.
SCIENTIFIC REPORTS
(2021)
Article
Horticulture
Zhenshan Liu, Jing Zhang, Yan Wang, Hao Wang, Lei Wang, Lu Zhang, Muran Xiong, Wen He, Shaofeng Yang, Qing Chen, Tao Chen, Ya Luo, Yong Zhang, Haoru Tang, Xiaorong Wang
Summary: In this study, a comparative genomics strategy was used to construct a database containing thousands of polymorphic SSR loci for genetic identification and trait-association analysis of Chinese cherry. The results showed that these markers exhibited high levels of informativeness and transferability, and were significantly associated with traits such as soluble solids and fruit size in Chinese cherry.
Article
Fisheries
Fan Sigang, Huang Hao, Liu Yong, Wang Pengfei, Zhao Chao, Yan Lulu, Qiao Xiuting, Qiu Lihua
Summary: A large number of SSRs were identified from the genome of the spotted sea bass, with 155,905 SSR markers successfully designed for assessing genetic diversity and cross-species transferability. The study also demonstrated high polymorphism information content (PIC) of most SSR markers and the potential for future genetic breeding and genomic studies of spotted sea bass.
AQUACULTURE REPORTS
(2021)
Article
Horticulture
Zhilei Wang, Ying Wang, Xiao Cao, Dong Wu, Miao Hui, Xing Han, Fei Yao, Yihan Li, Hua Li, Hua Wang
Summary: This study investigates the possibility of breeding disease-resistant grape varieties through hybridization and molecular marker techniques. The results show that the resistance to downy mildew is controlled by minor disease-resistance genes in V. vinifera and can be bred through intraspecific hybridization and recurrent selection.
Article
Plant Sciences
Huahao Jiang, Muhammad Waseem, Yong Wang, Sana Basharat, Xia Zhang, Yun Li, Pingwu Liu
Summary: Sugarcane is an important agricultural crop used for sugar production and biofuel. The genetic complexity of sugarcane makes it a challenging crop to improve. This study developed SSR markers from sugarcane ESTs, which can be used for genetic characterization and breeding efforts. Functional annotation analysis showed that the markers cover molecular, cellular, and biological pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Mukhtor M. Darmanov, Abdusalom K. Makamov, Mirzakamol S. Ayubov, Naim N. Khusenov, Zabardast T. Buriev, Shukhrat E. Shermatov, Ilkhom B. Salakhutdinov, Khurshida A. Ubaydullaeva, Jurabek K. Norbekov, Maftuna M. Kholmuradova, Sardor E. Narmatov, Ilyos S. Normamatov, Ibrokhim Y. Abdurakhmonov
Summary: Marker-assisted selection (MAS) is an effective method in cotton breeding to shorten breeding time, reduce resources and efforts. In this study, two novel MAS-derived cotton cultivars were developed by transferring LD-blocks with superior fiber quality QTLs from donor genotypes to recipient cultivars through backcrossing. The resulting cultivars showed improved fiber length, strength, uniformity and micronaire compared to the original parents. These results demonstrate the feasibility and success of MAS in cotton breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Aduragbemi Amo, Jose Miguel Soriano
Summary: Leaf rust resistance in wheat was studied by collecting 393 QTL from 50 mapping studies and identifying 15 high-confidence MQTL. This research provides valuable insights for developing resistant wheat cultivars.
Correction
Agronomy
Angela Polo-Oltra, Carlos Romero, Inmaculada Lopez, Maria Luisa Badenes, Elena Zuriaga
Article
Plant Sciences
Elena Zuriaga, Jitka Pintova, Julian Bartual, Maria Luisa Badenes
Summary: This study analyzed the genetic and morphological characteristics of a pomegranate germplasm collection. The results identified various genetic profiles that can be used for varietal identification and breeding. Further genetic analysis can help select suitable parental materials for hybridization and use molecular assisted selection to obtain breeding offspring.
Article
Genetics & Heredity
Ilaria Marcotuli, Jose Miguel Soriano, Agata Gadaleta
Summary: This study focused on identifying QTL hotspots in durum wheat for various quality traits using a collection of GWAS studies. Through the analysis of MTA on different chromosomes, a total of 94 QTL hotspots were identified, with candidate genes associated with biological processes controlling quality traits.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Venkata Rami Reddy Yannam, Marta Lopes, Carlos Guzman, Jose Miguel Soriano
Summary: This study conducted a genome-wide association analysis using 170 landraces from 24 Mediterranean countries, and identified QTL hotspots related to wheat quality and agronomic traits, as well as candidate genes. The accuracies of genomic prediction were calculated using cross-validation, and five prediction equations were developed to predict bread loaf volume.
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Agata Gadaleta, Jose Miguel Soriano
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Adrian Gracia-Romero, Ruben Rufo, David Gomez-Candon, Jose Miguel Soriano, Joaquim Bellvert, Venkata Rami Reddy Yannam, Davide Gulino, Marta S. Lopes
Summary: The development of accurate multivariate models for grain yield (GY) prediction in wheat experimental trials using normalized difference vegetation index (NDVI) and additional agronomic traits is a promising option to replace laborious in-field evaluations. This study proposed improved GY prediction models by incorporating NDVI, plant height, phenology, and ear density from experimental trials. The best models included NDVI, days to heading, and ear density or plant height, which showed a significant increase in prediction accuracy and a decrease in root mean square error.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Patricio Olmedo, Baltasar Zepeda, Joaquin Delgado-Rioseco, Carol Leiva, Adrian A. Moreno, Karen Sagredo, Francisca Blanco-Herrera, Romina Pedreschi, Rodrigo Infante, Claudio Meneses, Reinaldo Campos-Vargas
Summary: This study compared the changes in metabolite abundance in two nectarine varieties (Andes Nec-2 and Andes Nec-3) with different susceptibility to mealiness during cold storage using GC-MS analysis. The results showed that in unripe nectarines, cold storage led to an increase in the accumulation of amino acids, whereas in ripe nectarines, it resulted in a decrease in amino acid accumulation and an increase in the abundance of sugars and organic acids. Pathway reconstruction revealed that cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle in ripe nectarines, leading to differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines.
Article
Biology
Pietro M. Boselli, Jose M. Soriano
Summary: Every epidemic brings health, economic, social, and environmental problems that need to be addressed promptly. This study uses a mathematical model to analyze the trends of infected and deceased individuals, aiming to predict the duration and phases of the epidemic. The analysis of cumulative deaths provides more accurate forecasts of the epidemic's development.
Article
Plant Sciences
Tania Dorta, Francisco Gil-Munoz, Fany Carrasco, Elena Zuriaga, Gabino Rios, Manuel Blasco
Summary: This study investigated the involvement of effectors and transcriptional regulators in persimmon fruit maturation by analyzing the expression of genes involved in ethylene biosynthesis and response pathways. Results showed different gene expression patterns during fruit development, with some genes up-regulated and some genes down-regulated. The study also identified molecular mechanisms for high ethylene production in immature fruits and for fruit softening and skin colour change during natural ripening.
Article
Plant Sciences
Ana Puertes, Helin Polat, Luis Andres Ramon-Nunez, Matilde Gonzalez, Gema Ancillo, Elena Zuriaga, Gabino Rios
Summary: Transcriptomic and gene expression analysis help identify and characterize transcriptional regulatory factors and effectors in bud development. Single-bud gene analysis in peach flower buds reveals the variability in gene expression during dormancy release, contributing to the understanding of co-expression modules and regulatory aspects in this tissue.
Proceedings Paper
Agricultural Engineering
R. Infante
Summary: Chile has a long-standing tradition of exporting nectarines, but faced challenges with maintaining quality during long shipping periods. In response, a breeding program was initiated to develop cultivars suitable for extended postharvest periods.
X INTERNATIONAL PEACH SYMPOSIUM
(2022)
Article
Horticulture
Patricio Zapata, Makarena Gonzalez, Igor Pacheco, Claudia Jorquera, Claudia Silva-Andrade, Marco Isaac Garrido, Rodrigo Infante, Juan Alfonso Salazar
Summary: This study analyzed the molecular mechanisms involved in sexual differentiation in kiwifruit and identified key genes and pathways related to hormonal signaling pathways, development of floral organs, and biological and metabolic processes. The findings provide insights into the sex determination of kiwifruit and the development of flower organs.