Article
Biochemical Research Methods
Eeva-Christine Brockmann, Mikko Pyykko, Heidi Hannula, Kamran Khan, Urpo Lamminmaki, Tuomas Huovinen
Summary: This study discusses the impact of loop length variation on the structural diversity of antibody libraries. Introducing loop length variation in specific antibody fragments can enhance affinity, especially in CDR-L1 and CDR-H2 loops. The results lay the foundation for developing novel synthetic antibody libraries with unique loop length combinations.
Article
Microbiology
Shuai Hu, Guangbo Yang, Zhou Chen, Qiuye Li, Bin Liu, Ming Liu, Dawei Zhang, Shan Chang, Ren Kong
Summary: This study presents a method combining docking and site specific phage display to obtain single chain variable fragment (scFv) with high affinity to Salbutamol (SAL). Anti-SAL scFv antibodies were generated through phage display and new libraries were created by randomizing specific residues located on H3 and L3 based on docking results. High efficiency anti-SAL scFv antibodies were identified and used to develop a rapid and low-cost one step ELISA for SAL detection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jin-xing Song, Meng-xiang Wang, Yi-xuan Zhang, Bo Wan, Yong-kun Du, Guo-qing Zhuang, Zi-bin Li, Song-lin Qiao, Rui Geng, Ya-nan Wu, Gai-ping Zhang
Summary: This study generated an ASFV antibody phage-display library, screened a specific single-chain antibody against ASFV major capsid protein, and identified a conserved epitope peptide that can promote the maturation of immune cells and be effectively uptaken by dendritic cells.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Sukyo Jeong, Hyun Joo Ahn, Kyung Jin Min, Jae Won Byun, Hyun Mi Pyo, Mi Young Park, Bok Kyung Ku, Jinju Nah, Soyoon Ryoo, Sung Hwan Wee, Sang Jick Kim
Summary: A phage-displayed scFv library was constructed from FMDV-immune cattle, resulting in specific and high-affinity scFv antibodies for FMDV detection. Using natural host-derived antibodies such as bovine scFv in cELISA showed feasibility and effectiveness in serological detection of antibody response to FMDV in susceptible animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pierre-Emmanuel Baurand, Jeremy Balland, Chloe Reynas, Melanie Ramseyer, Delphine Vivier, Pierre-Simon Bellaye, Bertrand Collin, Catherine Paul, Franck Denat, Kamal Asgarov, Jean-Rene Pallandre, Laurence Ringenbach
Summary: The study developed an ScFv mouse phage-display library to obtain small fragment antibodies against human LRRC15 for molecular imaging purposes, demonstrating the relevance for the fast-track development of small antibodies for imaging and/or immunotherapy purposes. The library had a high insertion rate and enrichment through screening, leading to the identification of specific candidates for further development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Santiago Rodriguez, Aina Garcia-Garcia, Eduardo Garcia-Calvo, Vanesa Esteban, Carlos Pastor-Vargas, Araceli Diaz-Perales, Teresa Garcia, Rosario Martin
Summary: This study describes the use of phage display technology to generate a recombinant antibody for specific detection of hen's ovomucoid, addressing the need for reliable egg allergen detection methods. The developed phage enzyme-linked immunosorbent assay (ELISA) demonstrated good sensitivity and accuracy, and showed consistent results with a commercial egg detection assay in the analysis of various food products. In silico modeling of the antigen-antibody complex provided insights into the interaction mechanism.
Article
Immunology
Chao-Yang Huang, Ying-Yung Lok, Chia-Hui Lin, Szu-Liang Lai, Yen-Yu Wu, Chih-Yung Hu, Chu-Bin Liao, Chen-Hsuan Ho, Yu-Ping Chou, Yi-Hsuan Hsu, Yu-Hsun Lo, Edward Chern
Summary: In this study, a large and highly diverse synthetic human scFv antibody library was constructed, and novel TIM-3-neutralizing antibodies with immunomodulatory functions were identified. These findings demonstrate the potential of this library for biomedical research and the therapeutic potential of the three fully human TIM-3-neutralizing antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yaqi Yang, Siji Nian, Lin Li, Xue Wen, Qin Liu, Bo Zhang, Yu Lan, Qing Yuan, Yingchun Ye
Summary: Enhanced EphA2 expression is a crucial target for anti-tumor therapy, with high affinity scFvs against EphA2 successfully screened from an immune library constructed using PBMCs from cancer patients. Modified antibodies showed improved affinity and the ability to bind to EphA2, tumor cells, and tissues, inhibiting tumor growth to some extent. This study highlights the potential of immune libraries from cancer patients in screening for high-affinity antibodies with therapeutic effects.
Article
Chemistry, Analytical
Kui Gu, Peng Ma, Zengxu Song, Ming Yang, Xue Yang, Chao Li, Changyu Zhou, Zijing Ju, Yu Zhao, Hao Li, Xin Yang, Changwei Lei, Hongning Wang
Summary: African swine fever (ASF) is a highly contagious and lethal hemorrhagic disease, causing significant distress and economic losses to the world pig industry. The lack of approved vaccines and effective treatment makes accurate early detection of ASFV antigen and antibody crucial in preventing the outbreak and spread of ASFV. In this study, specific nanobodies against the ASFV-P30 protein were screened from an immunized Bactrian camel library and fused with horseradish peroxidase (HRP) for expression in HEK293T cells. A novel competitive ELISA (cELISA) based on ferritin-displayed P30 nanoparticles protein (P30-Fn) and nanobody-horseradish peroxidase fusions (P30-Nb71-vHRP) was developed for rapid and sensitive detection of ASFV antibody in serum samples, showing high sensitivity and specificity.
Review
Biochemical Research Methods
Rui Wang, Hui-Da Li, Ying Cao, Zi-Yi Wang, Ting Yang, Jian-Hua Wang
Summary: Viruses, specifically M13 phage, are being utilized in biosensing and biomedicine due to their versatility, specific reactivities, and responsiveness to genetic modifications. M13 phages can be engineered and modified to create a multifunctional analysis platform, with different functional regions performing their tasks without interference. The unique filamentous morphology and flexibility of M13 phages enhance target affinity and signal amplification in analytical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Toxicology
Hamideh Rouhani Nejad, Jalil Fallah Mehrabadi, Pardis Saeedi, Saeed Zanganeh
Summary: A novel, high specific, low-cost, and sensitive ScFv antibody was developed for rapid and specific detection of tetanus toxin in clinical diagnosis, suspicious foods, and water monitoring.
TOXICOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kui Gu, Zengxu Song, Peng Ma, Ziwei Liao, Ming Yang, Changyu Zhou, Chao Li, Yu Zhao, Hao Li, Xin Yang, Changwei Lei, Hongning Wang
Summary: A rapid, simple, sensitive, and specific competitive ELISA (cELISA) was developed in this study for the detection of Avian coronavirus-infectious bronchitis virus (AvCoV-IBV) antibodies. The developed cELISA showed high consistency with commercial ELISA, fast operation, and high sensitivity, making it potential for disease monitoring and control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Long Li, Shuangmin Wu, Yu Si, Huaming Li, Xiaoyang Yin, Dapeng Peng
Summary: The single-chain fragment variable (scFv) is a reliable, efficient, and accurate method for analyzing small-molecule harmful substances in food. It offers advantages over traditional antibodies and can be further improved through efficient construction, mutation, and expression measures.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Chemistry, Analytical
Yanbo Wang, Mingyang Wang, Haipeng Yu, Ge Wang, Pengxin Ma, Shuang Pang, Yiming Jiao, Aihua Liu
Summary: PSA is an important index for prostate cancer diagnosis and treatment. In this study, a biopanning method for PSA was established using a pIII phage display library. Two phage monoclonal antibodies specific to PSA were obtained, and an ELISA system was constructed for detecting tPSA with high accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Multidisciplinary
Jin Wang, Xing Shen, Peng Zhong, Zhaodong Li, Qiushi Tang, Xinan Huang, Anatoly V. Zherdev, Boris B. Dzantiev, Sergei A. Eremin, Zhili Xiao, Hongtao Lei, Xiangmei Li
Summary: A heterologous immunoassay strategy was designed to enhance the sensitivity for the detection of banned dye RB in fraudulent food in this study. The proposed immunoassay can be used for the surveillance screening of RB in a range of seasoning foods, indicating the heterologous strategy is an effective approach to enhance sensitivity in an immunoassay.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2021)