Article
Microbiology
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu
Summary: This study tested the stability of ten classic housekeeping genes under the pressure of eight commonly used antibiotics in the laboratory. Results showed that the stability of gene expression was indeed affected by the types of antibiotics added, and the best reference gene set varied for different antibiotics. This study provides a comprehensive summary of the effects of laboratory antibiotics on the stability of housekeeping genes in P. aeruginosa, highlighting the necessity to select housekeeping genes according to the type of antibiotics used in the initial stage of experiment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Conglong Lian, Bao Zhang, Jingfan Yang, JinXu Lan, Hao Yang, Kaihua Guo, Jingjing Li, Suiqing Chen
Summary: This study evaluated the expression stability of 12 candidate reference genes in different tissues and under different abiotic stresses in Isodon rubescens. The results identified the most stable reference genes for different tissues and treatments, and highlighted the importance of using appropriate reference genes in gene expression studies. This study provides valuable information for gene functional research in I. rubescens.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Gothandapani Sellamuthu, Jan Bily, Mallikarjuna Reddy Joga, Jiri Synek, Amit Roy
Summary: This study evaluated the stability of reference genes in Ips typographus under different experimental conditions and identified ribosomal protein 3a (RPS3-a) as the best reference gene. The findings contribute to future genomic and functional genomic research.
SCIENTIFIC REPORTS
(2022)
Article
Toxicology
Xiaoqi Zhao, Haiyun Chen, Yanan Zhu, Yi Liu, Lili Gao, Hui Wang, Ying Ao
Summary: This study aimed to screen suitable housekeeping genes for rat fetal kidney and identified stable housekeeping genes in the PDE-induced IUGR model as ACTB, GAPDH, TBP, and HMBS for males; and ACTB, YWHAZ, and GAPDH for females. The results suggest that ACTB + GAPDH are the best compound housekeeping genes for male fetal kidney studies, and ACTB + YWHAZ for females, providing experimental evidence for the selection of housekeeping genes in RT-qPCR experiments in renal development toxicology-related models.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lais Stehling de Queiroz Nascimento, Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Vinicius Carius Souza, Diego Silva Batista, Marcelo de Oliveira Santos, Wagner Campos Otoni, Lyderson Facio Viccini
Summary: Experimental conditions affect the expression of reference genes. NADH is the most stable gene under saline, exogenous hormones, wounding, and cold stress; whereas TUB is most stable under osmotic stress. Lippia alba is a medicinal plant with commercial value as a source of essential oils. RT-qPCR is a useful tool for studying gene expression and selecting improved essential oil composition. TUB and NADH are recommended as reference genes for expression studies in L. alba.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Multidisciplinary Sciences
Yang Hu, Qi Jiang, Xiang Zhai, Liang Liu, Yuntian Hong
Summary: This study screened and verified the optimal panel of reference genes in colorectal cancer and normal colonic cell lines. The results showed that the expression levels of candidate reference genes varied in different cell lines, but the optimal panel of reference genes always consisted of two genes. Moreover, the optimal combination of reference genes varied among different cell lines.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Joanna L. Fiddler, Stephen L. Clarke
Summary: This study assessed the stability of gene expression in various tissues obtained from iron-deficient and iron-replete pair-fed rats, finding that certain commonly used reference genes exhibited less stability compared to other candidate genes under different experimental conditions.
GENES AND NUTRITION
(2021)
Article
Multidisciplinary Sciences
Pramoda Kumar Sahoo, Sonali Parida, Subhadarshini Parida, Priyashree Parida, Anirban Paul
Summary: Argulosis is a economically significant freshwater fish ectoparasitic disease. Proper selection or normalization of reference genes is crucial for the accuracy of gene expression studies using real-time PCR. This study evaluated seven candidate reference genes and conducted comprehensive gene expression analysis in rohu carp infected with Argulus siamensis. The results identified stable and unstable reference genes and showed the role of immune modulation process in governing infection.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Gilar Gorji-Bahri, Niloofar Moradtabrizi, Atieh Hashemi
Summary: The study identified YWHAZ, UBC, and GAPDH as the most stable reference genes for breast cancer cell lines, while TBP exhibited the lowest stability in hepatic cancer cell lines. B2M and ACTB were identified as the least stable reference genes in breast cancer cell lines, while a panel of ACTB, HPRT1, UBC, YWHAZ, and B2M showed higher stability in hepatic cancer cell lines. The study's findings will assist researchers in selecting the most suitable combination of reference genes for experiments involving breast and hepatic cancer cell lines, with consideration of stability rankings provided.
Article
Multidisciplinary Sciences
Xiaoxing Yang, Guangxiang Tong, Le Dong, Ting Yan, Huan Xu, Guopan Tang, Yongquan Zhang, Kai Ma, Jiasheng Yin, Youyi Kuang
Summary: This study evaluated the stability of 10 commonly used candidate reference genes in taimen and identified RPS29 and RPL19 as the optimal reference genes for qRT-PCR analysis of gene expression in taimen.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Junliang Yin, Lu Hou, Xinchen Jiang, Jie Yang, Yang He, Xiaokang Zhou, Xiongmeng Zhu, Andong Gong, Yongxing Zhu, Zhongyi Chen
Summary: Alligatorweed is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance. This study identified stable reference genes for gene expression analysis in A. philoxeroides, laying a foundation for further research on gene expression profiling and invasive weed management.
Article
Biochemistry & Molecular Biology
Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Lais Stehling de Queiroz Nascimento, Lyderson Facio Viccini
Summary: Lippia alba is a well-studied plant species known for its medicinal properties, with gene expression studies requiring careful consideration for species with different ploidal levels. This study evaluated the stability of nine candidate reference genes in Lippia alba and identified CIT gene as a suitable stable housekeeping gene for polyploid studies. The results could be used as a reference not only for further gene expression studies in L. alba but also for other polyploid complexes.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Multidisciplinary Sciences
Manish Tiwari, Monika Sodhi, Preeti Verma, Prince Vivek, Ranjit S. Kataria, Saket K. Niranjan, Vijay K. Bharti, Nampher Masharing, Gayatri Gujar, Divya Chanda, Manishi Mukesh
Summary: This study evaluated the expression stability of 10 candidate reference genes in livestock species adapted to high altitude hypoxia conditions. Different panels of reference genes were identified for each species using various statistical algorithms. The findings of this study are important for understanding the molecular basis of high altitude adaptation in native livestock populations.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Matilde Calderoni, Maddalena Altare, Luca Mastracci, Federica Grillo, Laura Cornara, Aldo Pagano
Summary: This study demonstrates a method for qualitative identification of Peganum harmala seeds using optical and electron microscopy, as well as the development of a real-time qPCR test that can detect Peganum harmala DNA in quantities as low as less than 1 pg. In addition to species specificity and high sensitivity, this method accurately quantifies the presence of Peganum harmala seeds or seed parts in complex herbal mixtures, providing an indication of the danger level of the product.
Article
Multidisciplinary Sciences
Rong Li, Kaiwen Cui, Quanliang Xie, Shuangquan Xie, Xifeng Chen, Lu Zhuo, Aiping Cao, Haitao Shen, Xiang Jin, Fei Wang, Hongbin Li
Summary: This study identified stable reference genes from the transcriptome data of S. pennata, which play important roles in drought stress and development process. These results provide a reliable foundation for further investigations into the genetic responses of plants to abiotic stress.
SCIENTIFIC REPORTS
(2021)