Review
Biochemistry & Molecular Biology
Steven M. Heaton, Paul R. Gorry, Natalie A. Borg
Summary: Antiretroviral therapy (ART) is effective in reducing HIV-1 infection. However, the emergence of treatment-refractory variants remains a challenge. HIV-1 uses cellular helicases, including DDX3X, to facilitate its replication. Exploring these helicases may reveal new therapeutic targets and help prevent viral escape.
TRENDS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shringar Rao, Cynthia Lungu, Raquel Crespo, Thijs H. Steijaert, Alicja Gorska, Robert-Jan Palstra, Henrieke A. B. Prins, Wilfred van Ijcken, Yvonne M. Mueller, Jeroen J. A. van Kampen, Annelies Verbon, Peter D. Katsikis, Charles A. B. Boucher, Casper Rokx, Rob A. Gruters, Tokameh Mahmoudi
Summary: This study demonstrates the selective induction of cell death in latent HIV-1-infected cells by DDX3 inhibitors, and provides evidence for the reduction of the inducible HIV-1 reservoir in CD4+ T cells from PLWHIV. The pharmacological targeting of DDX3 shows promise in reversing HIV-1 latency and eliminating the latent reservoir.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Hung-Hsi Chen, Hsin- Yu, Rudy Rudy, Sim-Lin Lim, Yi-Fen Chen, Shu-Hsing Wu, Shu-Chun Lin, Muh-Hwa Yang, Woan-Yuh Tarn
Summary: The use of antibody arrays revealed that the RNA helicase DDX3 plays a role in modulating the expression of secreted signaling factors in oral squamous cell carcinoma (OSCC) cells. Furthermore, the interaction between DDX3 and the signal recognition particle (SRP) affects the expression of AREG mRNA, contributing to the progression of tumors.
Article
Biochemistry & Molecular Biology
Olga Sergeeva, Tatiana Abakumova, Ilia Kurochkin, Renata Ialchina, Anna Kosyreva, Tatiana Prikazchikova, Varvara Varlamova, Evgeniya Shcherbinina, Timofei Zatsepin
Summary: The study showed that a strong reduction of DDX3 protein leads to similar changes in vitro and in vivo, including cell cycle deregulation and pathway alterations, while a more moderate downregulation resulted in discordant results between in vitro and in vivo, indicating the significant impact of DDX3 protein levels on cell phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mingming Chen, Miwako Asanuma, Mari Takahashi, Yuichi Shichino, Mari Mito, Koichi Fujiwara, Hironori Saito, Stephen N. Floor, Nicholas T. Ingolia, Mikiko Sodeoka, Kosuke Dodo, Takuhiro Ito, Shintaro Iwasaki
Summary: The translation inhibitor Rocaglamide A shows promising antitumor activity by clamping eIF4A and DDX3 onto polypurine RNA for selective translational repression. Analysis of a de novo-assembled transcriptome from the natural source of RocA, plant Aglaia, reveals the amino acid critical for RocA binding. The dominant-negative effect of RocA on eIF4A and DDX3 leads to strong translational repression in cancer cells, indicating its tumor toxicity.
CELL CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jerome Laisney, Vanessa Loczenski Rose, Kayla Watters, Kevin Donohue, Jason M. Unrine
Summary: Research has shown that utilizing cerium oxide nanoparticles as a carrier successfully achieved oral delivery of shRNA, triggering RNA interference in stink bugs. By optimizing the properties of nanoparticles and the loading ratio of shRNA, successful reduction in target gene transcription levels was achieved, demonstrating the potential of nanoparticle delivery system to enhance the delivery of RNA-based biocontrols to stink bugs.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Microbiology
Thomas Kehrer, Anastasija Cupic, Chengjin Ye, Soner Yildiz, Mehdi Bouhaddou, Nicholas A. Crossland, Erika A. Barrall, Phillip Cohen, Anna Tseng, Tolga Cagatay, Raveen Rathnasinghe, Daniel Flores, Sonia Jangra, Fahmida Alam, Ignacio Mena, Sadaf Aslam, Anjali Saqi, Magdalena Rutkowska, Manisha R. Ummadi, Giuseppe Pisanelli, R. Blake Richardson, Ethan C. Veit, Jacqueline M. Fabius, Margaret Soucheray, Benjamin J. Polacco, Baran Ak, Arturo Marin, Matthew J. Evans, Danielle L. Swaney, Ana S. Gonzalez-Reiche, Emilia M. Sordillo, Harm van Bakel, Viviana Simon, Lorena Zuliani-Alvarez, Beatriz M. A. Fontoura, Brad R. Rosenberg, Nevan J. Krogan, Luis Martinez-Sobrido, Adolfo Garcia-Sastre, Lisa Miorin
Summary: The ORF6 protein of SARS-CoV-2 plays a crucial role in antagonizing the host's innate immunity by disrupting nuclear import and inhibiting cellular mRNA export. It also regulates viral protein expression. Mutations in ORF6 affect its interactions with host components and impair immune evasion in certain variants.
CELL HOST & MICROBE
(2023)
Article
Fisheries
Wen Shi, Na Wang, Bing Han, Mengmeng Zhang, Yanxue Shi, Dandan Zhao, Yanzi Zhang, Fengxia Zhao, Xueting Guan, Jinshan Huang, Min Liu
Summary: The DDX3 gene in chinook salmon plays a role in enhancing innate immunity and antiviral response by activating the type I IFN signaling pathway and inducing the expression of type I IFN. This study reveals the biological role of fish DDX3 in regulating innate immunity and antiviral effect, providing a scientific basis for understanding the antiviral regulation mechanism of innate immunity in fish. These findings can guide the breeding of disease-resistant salmon and prevent viral diseases.
Article
Biochemistry & Molecular Biology
Camila Pereira-Montecinos, Daniela Toro-Ascuy, Catarina Ananias-Saez, Aracelly Gaete-Argel, Cecilia Rojas-Fuentes, Sebastian Riquelme-Barrios, Barbara Rojas-Araya, Francisco Garcia-de-Gracia, Paulina Aguilera-Cortes, Jonas Chnaiderman, Monica L. Acevedo, Fernando Valiente-Echeverria, Ricardo Soto-Rifo
Summary: This study reveals the regulatory role of N-6-methyladenosine (m(6)A) deposition on HIV-1 full-length RNA packaging, as well as the interaction between HIV-1 Gag protein and RNA demethylase FTO. The results show that m(6)A deposition leads to increased Gag synthesis and reduced packaging, while FTO-mediated demethylation promotes the incorporation of the full-length RNA into viral particles.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Virology
Margarita. I. Zarudnaya, Andriy. L. Potyahaylo, Iryna. M. Kolomiets, Leonid. G. Gorb
Summary: HIV-1 RNA dimerization is a crucial step in the virus's life cycle, and it is facilitated by the DIS hairpin. The DIS hairpin plays a role in genome packaging, reverse transcription, and recombination. Through studying HIV-1 M genomes, researchers have identified five common apical loops in the DIS hairpin and classified them into five dimerization groups. Additionally, it was found that the DIS, SD, and Psi hairpins have numerous variants, with only a few occurring frequently. The G x AGG internal loop in the lower part of the DIS hairpin is highly conserved in both HIV-1 and SIV genomes. It is speculated that the G-quadruplex downstream of the Gag start codon may be involved in the switch of HIV-1 leader RNA's conformation.
Article
Virology
Aparajitha Vaidyanathan, Harry E. Taylor, Thomas J. Hope, Richard T. D'Aquila, Elizabeth T. Bartom, Judd F. Hultquist, Marcus E. Peter
Summary: HIV infection leads to depletion of CD4(+) T cells, but some cells can survive and become part of the latent reservoir. Understanding the mechanisms of HIV-mediated cell death could potentially help clear the latent reservoir.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Gatikrushna Singh, Bradley Seufzer, Zhenwei Song, Dora Zucko, Xiao Heng, Kathleen Boris-Lawrie
Summary: This study reveals the relationship between incomplete processing and hypermethylation of HIV-1 transcripts, as well as the role of trimethylguanosine synthetase-1 in HIV-1 infectivity and protein synthesis. The study also uncovers the interaction between nuclear RNA helicase A and the shape of the 5' untranslated region primer binding site, and the relationship between HIV-1 virion protein synthesis and host translation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Junxiu Wang, Tingting Li, Sufang Deng, Enbo Ma, Jianzhen Zhang, Shuping Xing
Summary: The study reveals that LmDDX3 plays a critical role in regulating the transcription of vitellogenin and vitellogenin receptor, two key factors required for ovary development and oocyte maturation in locust. It provides a new potential target for locust biological control.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kannan Balakrishnan, Punnagai Munusami, Krishnaveni Mohareer, U. Deva Priyakumar, Atoshi Banerjee, Tom Luedde, Shekhar C. Mande, Carsten Munk, Sharmistha Banerjee
Summary: Nucleocytoplasmic shuttling of viral elements is essential for HIV replication. In this study, a complex formation between Staufen-2, CRM1, and Rev was identified, and the interaction residues in Staufen-2 were mapped. Mutations disrupting the interaction impaired Rev-RNA export activity and viral production. The findings highlight the importance of these interactions in enhancing RNA export activity and reducing HIV-1 proliferation.
Article
Microbiology
Han Xiao, Emanuel Wyler, Miha Milek, Bastian Grewe, Philipp Kirchner, Arif Ekici, Ana Beatriz Oliveira Villela Silva, Doris Jungnickl, Florian Full, Marco Thomas, Markus Landthaler, Armin Ensser, Klaus Ueberla
Summary: The HIV-1 Rev protein is crucial for nuclear export of unspliced and incompletely spliced HIV-1 RNAs. Depletion of proteins like CRNKL1 can significantly enhance levels of unspliced HIV-1 RNA in the cytoplasm, potentially revealing a cellular mechanism exploited by HIV-1 to regulate its complex splicing pattern.
Review
Virology
Yu Chen, Qianyun Liu, Deyin Guo
JOURNAL OF MEDICAL VIROLOGY
(2020)
Letter
Medicine, General & Internal
Shibo Jiang, Zhengli Shi, Yuelong Shu, Jingdong Song, George F. Gao, Wenjie Tan, Deyin Guo
Article
Virology
Deyin Guo
Article
Cell Biology
Panpan Hou, Kongxiang Yang, Penghui Jia, Lan Liu, Yuxin Lin, Zibo Li, Jun Li, Shuliang Chen, Shuting Guo, Junyu Wu, Hong Peng, Weijie Zeng, Chunmei Li, Yingfang Liu, Deyin Guo
Summary: CCDC50 is a newly discovered autophagy receptor that negatively regulates the type I interferon signaling pathway by targeting K63-polyubiquitinated RLRs for autophagic degradation, which enhances viral resistance and survival rates in mouse models.
Article
Virology
Junwei Li, Mingyue Song, Deyin Guo, Yongxiang Yi
Article
Biochemistry & Molecular Biology
Yuxin Lin, Zibo Li, Yicheng Wang, Tian Tian, Penghui Jia, Yu Ye, Miao He, Zixiao Yang, Chunmei Li, Deyin Guo, Panpan Hou
Summary: This study identifies a novel autophagy cargo receptor, CCDC50, which negatively regulates NLRP3 inflammasome assembly and the release of related inflammatory factors by degrading NLRP3. CCDC50 deficiency results in upregulation of signaling pathways associated with autoinflammatory diseases and enhances gut inflammation response.
Review
Virology
Wenhao Wang, Xiaoxue Peng, Yunyun Jin, Ji-An Pan, Deyin Guo
Summary: The ongoing COVID-19 pandemic has led to severe public health crises and economic losses. Limited knowledge about the SARS-CoV-2 virus calls for more research. Reverse genetics systems, like viral infectious clones and replicons, provide powerful platforms for various viral research projects. The development of noninfectious replicons allows for safer operations in more widely available Biosafety Level 2 (BSL2) facilities. After the outbreak, multiple reverse genetics systems for SARS-CoV-2 have been developed, offering researchers various options for their studies.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Jingshu Xiao, Jiaming Liang, Junjie Fan, Panpan Hou, Xiaodong Li, Haipeng Zhang, Kai Li, Lang Bu, Ping Li, Miao He, Yongheng Zhong, Liping Guo, Penghui Jia, Qiaoqiao Xiao, Junyu Wu, Hong Peng, Chunmei Li, Fan Xing, Deyin Guo
Summary: This study presents a promising strategy for the treatment of aggressive glioblastoma (GBM) by combining CDK4/6 inhibitors with oncolytic viruses (OV). CDK4/6 inhibition enhances the efficacy of OV by promoting autophagic degradation of MAVS, impairing antiviral responses, and amplifying oncolysis. Furthermore, CDK4/6 inhibitors and OV synergistically induce immuno-genic cell death and boost antitumor immunity, inhibiting tumor growth and prolonging survival in GBM models.
Letter
Cell Biology
Lihong Liu, Birong Zheng, Manhui Luo, Jie Du, Fang Yang, Chuanxin Huang, Zengyi Ma, Chunmei Li, Deyin Guo, Hong Peng
Article
Microbiology
Hongchang Dong, Wencheng Wu, Jingjing Li, Yilei Ma, Xiaomei Deng, Deyin Guo, Pei Xu
Summary: This study provides detailed insights into the signal transduction pathways of PML bodies in response to IFN and virus infection. It reveals the involvement of the constitutive component Sp100A in mediating these processes and highlights the importance of nuclear importation of Sp100A in activating essential antiviral genes.
Letter
Biochemistry & Molecular Biology
Liping Guo, Xue Liu, Yuchen Gu, Jinyan Jiang, Ziyue Yang, Qiuying Lv, Deyin Guo, Yang Yang, Hongzhou Lu, Jing Yuan
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Qifan Zhou, Sidi Yang, Liu Cao, Yang Yang, Tiefeng Xu, Qishu Chen, Hongzhou Lu, Yingjun Li, Deyin Guo, Xumu Zhang
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Xiangpeng Sheng, Fang Zhu, Hong Peng, Fan Yang, Yi Yang, Cong Yang, Zhen Wang, Wei Chen, Deyin Guo, Ronggui Hu
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Lang Bu, Huan Wang, Ji-an Pan, Lang Chen, Fan Xing, Junyu Wu, Shun Li, Deyin Guo
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Letter
Biochemistry & Molecular Biology
Fan Xing, Jingshu Xiao, Junyu Wu, Jiaming Liang, Xiaoyu Lu, Liping Guo, Ping Li, Panpan Hou, Chunmei Li, Deyin Guo
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)