Article
Chemistry, Analytical
Zhidan Liang, Morgan B. Giles, Mallory J. Stenslik, Maureen Marsales, James D. Ormes, Ray Seto, Wendy Zhong
Summary: This study presents a novel application of matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for visualizing the drug release process from non-conductive polymeric LAP implants. The researchers developed a new sample surface treatment procedure to obtain homogeneous imaging of non-conductive sample surfaces. This work is the first comprehensive report of a sample preparation methodology tailored for imaging LAP at the molecular level.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Eva Illes-Toth, Oliver J. Hale, James W. Hughes, Nicole Strittmatter, Jonathan Rose, Ben Clayton, Rebecca Sargeant, Stewart Jones, Andreas Dannhorn, Richard J. A. Goodwin, Helen J. Cooper
Summary: This study demonstrates for the first time the in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo, and provides a direct route for target-drug characterization in early stage drug discovery by spatial mapping across liver tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Michael S. Westphall, Kenneth W. Lee, Colin Hemme, Austin Z. Salome, Keaton Mertz, Timothy Grant, Joshua J. Coon
Summary: This article describes an apparatus that can cryogenically land particles from the ion beam of a mass spectrometer onto transmission electron microscope grids for cryo-electron microscopy. This system also allows for the controlled formation of thin films of amorphous ice on the grid surface. The study demonstrates that using this cryogenic landing device significantly improves the structural preservation of deposited protein-protein complexes compared to room temperature landings, and landing under cryogenic conditions can increase the diversity of particle orientations, enabling improved 3D structural interpretation.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Claudia Buhigas, Anne Y. Warren, Wing-Kit Leung, Hayley C. Whitaker, Hayley J. Luxton, Steve Hawkins, Jonathan Kay, Adam Butler, Yaobo Xu, Dan J. Woodcock, Sue Merson, Fiona M. Frame, Atef Sahli, Federico Abascal, Inigo Martincorena, G. Steven Bova, Christopher S. Foster, Peter Campbell, Norman J. Maitland, David E. Neal, Charlie E. Massie, Andy G. Lynch, Rosalind A. Eeles, Colin S. Cooper, David C. Wedge, Daniel S. Brewer
Summary: This study suggests that cells within normal prostate tissue can expand through mechanisms distinct from cancer, likely originating from stromal cells. Compared to cancer, normal tissue has fewer single nucleotide variants, almost no structural variants or copy number alterations. These findings have significant implications for treatment and early detection approaches.
Review
Biochemistry & Molecular Biology
Mallika Khurana, Syed Obaidur Rahman, Shivani Agarwal, Md. Sayeed Akhtar, Mohammed S. Aldughaim, Faheem Hyder Pottoo, Abul Kalam Najmi
Summary: Research on neurodegenerative diseases has highlighted the importance of discovering biomarkers for accurate and reproducible diagnosis and prognosis. Evolving proteomic technologies, such as Mass Spectrometry, offer new avenues for rapidly identifying biomarkers, but further investigation is needed to explore the complexities of the brain and its disorders.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Catia Costa, Veronique Dartois, Firat Kaya, Mark Chambers, Janella de Jesus, Vladimir Palitsin, Roger Webb, Melanie J. Bailey
Summary: This study combines elemental imaging and a new method for spatially resolved lipidomics to investigate the relationship between metals, drugs, and lipids in different areas of tissues. The method was applied to rabbit lung tissues with tuberculosis infection and demonstrated the association between ion accumulation, lipid profiles, and local drug distribution.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Juliana Pereira Lopes Goncalves, Christine Bollwein, Aurelia Noske, Anne Jacob, Paul Jank, Sibylle Loibl, Valentina Nekljudova, Peter A. A. Fasching, Thomas Karn, Frederik Marme, Volkmar Mueller, Christian Schem, Bruno Valentin Sinn, Elmar Stickeler, Marion van Mackelenbergh, Wolfgang D. D. Schmitt, Carsten Denkert, Wilko Weichert, Kristina Schwamborn
Summary: Immunohistochemical evaluation of breast cancer subtypes using estrogen receptor, progesterone receptor, and HER2 status is important for treatment determination. This study utilized mass spectrometry imaging to identify molecular features and accurately classify triple-negative breast cancer (TNBC). The models developed demonstrated high accuracy for hormone receptors and TNBC classification, and vimentin overexpression was found to be a potential target for diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luis-Manuel Quiros-Guerrero, Louis-Felix Nothias, Arnaud Gaudry, Laurence Marcourt, Pierre-Marie Allard, Adriano Rutz, Bruno David, Emerson Ferreira Queiroz, Jean-Luc Wolfender
Summary: Collections of natural extracts have the potential to discover novel natural products, but the prioritization of extracts remains challenging. Inventa is a computational tool that considers mass spectrometry data, spectral annotation, and literature reports to highlight the structural novelty potential within extracts. Application of Inventa to plants from the Celastraceae family identified the roots extract of Pristimera indica as a promising source of potentially novel compounds.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Ondrej Kuda, Oliver Fiehn, Tomas Cajka
Summary: Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts was investigated over a three-day period under various temperature conditions. Results showed that storing dry extracts at -24 degrees C provided comparable results to -80 degrees C, but increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 degrees C and +30 degrees C.
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Weiyu Li, Chunyan Hou, Yaoxiang Li, Ci Wu, Junfeng Ma
Summary: Protein glycosylation is important for regulating biological processes, and mass spectrometry-based analysis is crucial for understanding these events. However, distinguishing structural isomers of glycans and glycopeptides remains challenging. In this study, a binary logistic regression model was established to classify O-GlcNAc/O-GalNAc peptides, achieving high accuracy (>87%). A corresponding web server, HexNAcQuest, was developed for easy integration in glycoproteomics workflows.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Environmental Sciences
Borhan Mansouri, Zana Ramezani, Vahid Yousefinejad, Samaneh Nakhaee, Namamali Azadi, Peyman Khaledi, Bahram Nikkhoo, Kambiz Hassanzadeh, Azin Rahimi
Summary: The study revealed significantly elevated levels of Pb, Cd, As, Cr, Cu, and Se in breast cancerous tissues, while Zn was higher in non-cancerous tissues. Weak to moderate correlations between elements were observed in the cancerous group, with no significant correlations found in the non-cancerous group. Raw risk differences indicated a significant effect of Pb, Cd, As, Ag, Cr, Se, and Zn on the development of breast cancer.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Martina Zandl-Lang, Barbara Plecko, Harald Koefeler
Summary: Although Next-Generation Sequencing (NGS) has helped identify disease-associated mutations, the diagnostic rate of rare diseases is still low. Improving the diagnostic rate and reducing time to diagnosis in children with unclear diseases is crucial. Analyzing lipids in biological samples provides important insights into their functional role in health and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Nicole Strittmatter, Frances M. Richards, Alan M. Race, Stephanie Ling, Daniel Sutton, Anna Nilsson, Yann Wallez, Jennifer Barnes, Gareth Maglennon, Aarthi Gopinathan, Rebecca Brais, Edmond Wong, Maria Paola Serra, James Atkinson, Aaron Smith, Joanne Wilson, Gregory Hamm, Timothy Johnson, Charles R. Dunlop, Brajesh P. Kaistha, Josephine Bunch, Owen J. Sansom, Zoltan Takats, Per E. Andren, Alan Lau, Simon T. Barry, Richard J. A. Goodwin, Duncan Jodrell
Summary: Gemcitabine distribution and metabolism were assessed using a multimodal imaging approach, revealing that active metabolites primarily reached tumor cell compartments, while an ATR inhibitor targeted nonviable tumor regions.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Wenxing Gu, Shenhua Li, Yajie Yang, Shumin Wang, Kai Li, Yongsheng Zhao, Jing Mu, Xiaoyuan Chen
Summary: This review summarizes recent advances in ROS-responsive probes for in vivo imaging and diagnosis of various physiological system diseases, and discusses the current challenges and perspectives in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Kelly M. Hines, Josi Herron, Libin Xu
JOURNAL OF LIPID RESEARCH
(2017)
Article
Microbiology
Kelly M. Hines, Adam Waalkes, Kelsi Penewit, Elizabeth A. Holmes, Stephen J. Salipante, Brian J. Werth, Libin Xu
Correction
Multidisciplinary Sciences
Steven J. Fliesler, Neal S. Peachey, Josi Herron, Kelly M. Hines, Nadav I. Weinstock, Sriganesh Ramachandra Rao, Libin Xu
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Steven J. Fliesler, Neal S. Peachey, Josi Herron, Kelly M. Hines, Nadav I. Weinstock, Sriganesh Ramachandra Rao, Libin Xu
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Kelly M. Hines, Libin Xu
CHEMISTRY AND PHYSICS OF LIPIDS
(2019)
Article
Biochemistry & Molecular Biology
David R. Raleigh, Navdar Sever, Pervinder K. Choksi, Monika Abedin Sigg, Kelly M. Hines, Bonne M. Thompson, Daniel Elnatan, Priyadarshini Jaishankar, Paola Bisignano, Francesc R. Garcia-Gonzalo, Alexis Leigh Krup, Markus Eberl, Eamon F. X. Byrne, Christian Siebold, Sunny Y. Wong, Adam R. Renslo, Michael Grabe, Jeffrey G. McDonald, Libin Xu, Philip A. Beachy, Jeremy F. Reiter
Article
Toxicology
Josi M. Herron, Kelly M. Hines, Hideaki Tomita, Ryan P. Seguin, Julia Yue Cui, Libin Xu
TOXICOLOGICAL SCIENCES
(2019)
Article
Microbiology
Aurore Fleurie, Abdelrahim Zoued, Laura Alvarez, Kelly M. Hines, Felipe Cava, Libin Xu, Brigid M. Davis, Matthew K. Waldor
Article
Biochemistry & Molecular Biology
Marley Brimberry, Marina Ana Toma, Kelly M. Hines, William N. Lanzilotta
Summary: In response to increasing antibiotic resistance and growing recognition of the human microbiome, new therapeutic targets need to be identified. This study investigated the unique metabolic pathways of Vibrio cholerae, particularly the catalytic role of HutW in the anaerobic opening of the porphyrin ring of heme.
Article
Biochemical Research Methods
Christian Freeman, Hannah M. Hynds, Jana M. Carpenter, Keerthi Appala, Kingsley Bimpeh, Shannon Barbarek, Craig Gatto, Brian J. Wilkinson, Kelly M. Hines
Summary: Up to 80% of Staphylococcus aureus membrane lipids consist of branched-chain fatty acids, which may have either even or odd numbers of carbons with different branch positions. This complexity makes it difficult to differentiate isomeric fatty acid species with the same number of carbons using mass spectrometry.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Kelly M. Hines, Hannah M. Hynd
Summary: The use of halogenated PB reagents and ion mobility technique improves the identification of PB-derivatized lipids. 4'-chloro-2', 6'-difluoroacetophenone (CDFAP) shows the best performance, reducing the collision cross-section (CCS) values of derivatized lipids and yielding sufficient intensity for both the derivatized lipid and its diagnostic ions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Kingsley Bimpeh, Kelly M. Hines
Summary: The lipid membrane plays a critical role in the emergence of antibiotic resistance. The commonly used two-phase liquid-liquid extractions for lipid recovery in bacteria are cumbersome and can lead to poor reproducibility or contaminant introduction. This research proposes a simple, single-phase lipid extraction method called the MAW method, which achieves comparable recoveries to the Bligh & Dyer method but with the benefit of easier detection of lipids from lower amounts of bacteria, suitable for high-throughput studies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Dylan H. Ross, Jang Ho Cho, Rutan Zhang, Kelly M. Hines, Libin Xu
ANALYTICAL CHEMISTRY
(2020)
Article
Medicine, Research & Experimental
Elijah J. Weber, Kevin A. Lidberg, Lu Wang, Theo K. Bammler, James W. MacDonald, Mavis J. Li, Michelle Redhair, William M. Atkins, Cecilia Tran, Kelly M. Hines, Josi Herron, Libin Xu, Maria Beatriz Monteiro, Susanne Ramm, Vishal Vaidya, Martti Vaara, Timo Vaara, Jonathan Himmelfarb, Edward J. Kelly
Article
Chemistry, Analytical
Kelly M. Hines, Dylan H. Ross, Kimberly L. Davidson, Matthew F. Bush, Libin Xu
ANALYTICAL CHEMISTRY
(2017)