Article
Biochemical Research Methods
Jaeyoung Kang, Weihong Xu, Wout Bittremieux, Niema Moshiri, Tajana Rosing
Summary: Driven by technological advances, mass spectrometry (MS) proteomics experiments have greatly improved in throughput and cost. Spectral library searching is commonly used for annotating experimental mass spectra, but it has the disadvantage of only identifying known peptides. Open modification searching (OMS) is an increasingly popular approach to annotate modified peptides, but it suffers from large search spaces and long runtimes.
Article
Horticulture
Heliofabia Virginia de Vasconcelos Facundo, Gabriela Justamante Handel Schmitz, Thais Regiani Cataldi, Carlos Alberto Labate, Joao Roberto Oliveira do Nascimento, Franco Maria Lajolo
Summary: This study investigated the effect of low temperature storage on the proteome of banana cultivars Nanicao and Prata. The results showed differences in protein expression between the two cultivars in response to low temperatures, which may explain their different tolerance levels.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Ju-Young Jung, Cheol Woo Min, Jeong Woo Jang, Ravi Gupta, Ji-Hyun Kim, Young-Hun Kim, Sung Won Cho, Young Hun Song, Ick-Hyun Jo, Randeep Rakwal, Yu-Jin Kim, Sun Tae Kim
Summary: This study reveals the proteome changes in ginseng leaves in response to salt stress and identifies the salt-responsive protein, PgGH17, which improves salt tolerance when heterologously expressed in Arabidopsis thaliana.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Antonio Maria Fea, Martina Menchini, Alessandro Rossi, Chiara Posarelli, Lorenza Malinverni, Michele Figus
Summary: The study investigated the efficacy and safety of the XEN63 implant in open-angle glaucoma patients, showing significant reduction in intraocular pressure and the need for hypotensive medications, with good short-term safety profile.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
Ya-Hui Wei, Yu Cai, Bonnie N. K. Choy, Bai-Bing Li, Ruo-Shi Li, Chen Xing, Xia Wang, Tian Tian, Yuan Fang, Mei Li, Ying-Zi Pan
Summary: NTG patients showed higher DCR values compared to controls, with some DCRs being negatively correlated with CCT and IOP, while others were positively correlated with these factors. The cornea was more deformable in NTG compared to HTG or controls, indicating a potential biomechanical mechanism underlying glaucomatous optic neuropathy in NTG patients.
CHINESE MEDICAL JOURNAL
(2021)
Article
Ophthalmology
Jing Na He, Tsz Kin Ng, Shi Yao Lu, Pancy Oi Sin Tam, Poemen P. Chan, Clement C. Tham, Chi Pui Pang, Li Jia Chen, Wai Kit Chu
Summary: This study investigated the association between the ANGPT2 gene and primary open-angle glaucoma (POAG) in the Chinese population. The results showed that the SNP rs11775442 was associated with normal tension glaucoma (NTG) in the Hong Kong cohort, while rs13269021 was associated with NTG in males in the Shantou cohort. Further studies are needed to confirm the association between the ANGPT2 locus and NTG in Chinese males.
Article
Medicine, General & Internal
Michele Lanza, Angelo Leone, Gabriele Scognamiglio, Luigi Serra, Clemente Maria Iodice, Paolo Melillo, Francesca Simonelli
Summary: This study found no significant differences in the efficacy of first-choice medications for primary open-angle glaucoma, but the efficacy may be influenced by various ocular and systemic features in different groups.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Ophthalmology
Brian S. Cole, Harini V. Gudiseva, Maxwell Pistilli, Rebecca Salowe, Caitlin P. McHugh, Michael C. Zody, Venkata R. M. Chavali, Gui Shuang Ying, Jason H. Moore, Joan M. O'Brien
Summary: This study examines the association of autosomal ancestry with POAG risk in a large cohort of self-identified African Americans. The results show that a higher degree of African ancestry is associated with an increased risk of POAG. Additionally, it is found that both known POAG single nucleotide polymorphisms and an omnigenic ancestry effect influence POAG risk.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Medicine, General & Internal
Faisal A. Almobarak, Ahmed Alrubean, Waleed Alsarhani, Abdullah Aljenaidel, Essam A. Osman
Summary: Ultrasound cyclo plasty (UCP) is effective in controlling intraocular pressure (IOP) and reducing the need for antiglaucoma medications in primary open-angle glaucoma (POAG) eyes. However, the success rate is modest, with higher baseline IOP and number of medications associated with a higher risk of failure.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Kinga Golaszewska, Iwona Obuchowska, Joanna Konopinska
Summary: This study evaluated the efficacy and safety profile of iStent bypass implantation and ab externo canaloplasty in POAG patients, and found that both procedures effectively reduced intraocular pressure and the need for anti-glaucoma medications.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Sarah Zhou, Bruce Burkemper, Anmol A. Pardeshi, Galo Apolo, Grace Richter, Xuejuan Jiang, Mina Torres, Roberta McKean-Cowdin, Rohit Varma, Benjamin Y. Xu
Summary: This study analyzed epidemiological data of populations in Latino and Chinese Americans, and assessed the differences in the roles of refractive error (RE) and ocular biometrics as risk factors for primary open-angle glaucoma (POAG) by race/ethnicity. The results showed that while the risks of high myopia and longer axial length were similar for POAG in Latino and Chinese Americans, high myopia was more prevalent among Chinese Americans, resulting in a narrowed difference in POAG prevalence between the two groups.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Plant Sciences
Rungcharn Suksungworn, Sittiruk Roytrakul, Nelson G. M. Gomes, Sutsawat Duangsrisai
Summary: The study revealed that coumarins affect protein expression in giant mimosa seedlings, interfering with proteins related to cellular component organization, metabolic processes, and playing important roles in plant growth and development, cell wall responses, Ca2+ accumulation, and programmed cell death.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Review
Ophthalmology
Harathy Selvan, Shikha Gupta, Janey L. Wiggs, Viney Gupta
Summary: Juvenile-onset open-angle glaucoma (JOAG) is a specific type of glaucoma that is diagnosed before the age of 40. It can be familial or non-familial, with myocilin mutations being the most common association. JOAG is characterized by high intraocular pressures and often requires surgery. Early diagnosis, prompt management, and lifelong monitoring are crucial in preventing disease progression.
SURVEY OF OPHTHALMOLOGY
(2022)
Article
Health Care Sciences & Services
Wei-Yang Lu, Ci-Wen Luo, Shyan-Tarng Chen, Yu-Hsiang Kuan, Shun-Fa Yang, Han-Yin Sun
Summary: The study found that comorbidities such as hypotension, sleep disturbances, peptic ulcers, and allergic rhinitis are more highly associated with NTG than POAG.
Article
Medicine, General & Internal
Maria D. Pinazo-Duran, Vicente Zanon-Moreno, Carolina Garcia-Villanueva, Alessio Martucci, Cristina Peris-Martinez, Jorge Vila-Arteaga, Jose J. Garcia-Medina, Irene Andres-Blasco, Alex Gallego-Martinez, Carlo Nucci, Julian Garcia-Feijoo
Summary: Glaucoma is a chronic neurodegenerative disease and the leading cause of irreversible blindness worldwide. Finding new glaucoma biomarkers, especially in early stages, and monitoring treatment response are crucial for improving vision outcomes.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Beatrice Dufrusine, Verena Damiani, Emily Capone, Damiana Pieragostino, Enrico Dainese, Margot De Marco, Francesca Reppucci, Maria C. Turco, Alessandra Rosati, Liberato Marzullo, Gianluca Sala, Michele Sallese, Vincenzo De Laurenzi
Summary: The study demonstrates that BAG3 activates human fibroblasts to release cytokines supporting the growth and migration of PDAC cells. Blocking the BAG3 axis inhibits cytokine release and migratory phenotype in PDAC cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Damiana Pieragostino, Manuela Lanzini, Ilaria Cicalini, Maria Concetta Cufaro, Verena Damiani, Leonardo Mastropasqua, Vincenzo De Laurenzi, Mario Nubile, Paola Lanuti, Giuseppina Bologna, Luca Agnifili, Piero Del Boccio
Summary: This study used tears proteomics to analyze protein expression during pharmacological treatment of Neurotrophic Keratopathy (NK). The results showed that the treatment induced an endogenous inflammatory response and improved the condition by stimulating protein synthesis and nerve fiber regeneration.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Marina Mingoia, Carmela Conte, Annalisa Di Rienzo, Marilisa Pia Dimmito, Lorella Marinucci, Gloria Magi, Hasan Turkez, Maria Concetta Cufaro, Piero Del Boccio, Antonio Di Stefano, Ivana Cacciatore
Summary: This study reports the synthesis of novel cinnamic acid-based antimicrobials (DM1-11) and their potential as antibacterial drugs for the treatment of ESKAPE-related skin infections. DM2 shows significant antibacterial activity and wound healing properties, making it a potentially safe topical agent for wound treatment.
Editorial Material
Biochemistry & Molecular Biology
Maurine Fucito, Damiana Pieragostino
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Verena Damiani, Maria Concetta Cufaro, Maurine Fucito, Beatrice Dufrusine, Claudia Rossi, Piero Del Boccio, Luca Federici, Maria Caterina Turco, Michele Sallese, Damiana Pieragostino, Vincenzo De Laurenzi
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer mortality worldwide. This study investigated the crosstalk between PDAC cells and associated fibroblasts. Proteomic analysis was used to identify proteins released from PDAC cells and detect proteostatic changes in normal fibroblasts treated with PDAC conditioned medium. The results suggest that the activation of migration, vasculogenesis, cellular homeostasis, metabolism of amino acids, and reduced apoptosis may be regulated by ITGB3 and TGFB1/2 followed by SMAD3, STAT3, and BAG3 activation.
Article
Biochemistry & Molecular Biology
Ilaria Cela, Maria Concetta Cufaro, Maurine Fucito, Damiana Pieragostino, Paola Lanuti, Michele Sallese, Piero Del Boccio, Adele Di Matteo, Nerino Allocati, Vincenzo De Laurenzi, Luca Federici
Summary: The role of nucleostemin in acute myeloid leukemia (AML) is unclear. Using a proteomics approach, researchers found that silencing nucleostemin in AML cells had effects on DNA repair and increased DNA damage rates, indicating its potential importance in AML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Simone Vespa, Pasquale Simeone, Giulia Catitti, Davide Buca, Domenico De Bellis, Laura Pierdomenico, Damiana Pieragostino, Ilaria Cicalini, Piero Del Boccio, Luca Natale, Trevor Owens, Reza Khorooshi, Vincenzo De Laurenzi, Liborio Stuppia, Paola Lanuti
Summary: Recent studies have focused on the protective and/or pathological role of virus-specific T cells in SARS-CoV-2 infection. This study found that the immune response produced by natural infection is similar to that induced by vaccination. All vaccinated individuals showed the production of memory T cells, which exhibited sustained polyfunctionality against the virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Paola Simeone, Rossella Liani, Romina Tripaldi, Sonia Ciotti, Antonio Recchiuti, Vittorio Abbonante, Benedetta Porro, Piero Del Boccio, Augusto di Castelnuovo, Paola Lanuti, Marina Camera, Damiana Pieragostino, Melissa Lee-Sundlov, Myriam Luongo, Raffaella Auciello, Giuseppina Bologna, Maria Concetta Cufaro, Elena Tremoli, Karin M. Hoffmeister, Francesco Cipollone, Alessandra Balduini, Francesca Santilli
Summary: Prevention of cardiovascular disease with low-dose aspirin may be less effective in patients with faster recovery of platelet cyclooxygenase-1 activity. Patients with accelerated COX-1 recovery exhibit reduced platelet glycoprotein Ib alpha shedding, leading to increased thrombopoietin production and the formation of platelets that are not inhibited by aspirin over 24 hours. Clinical markers including age, non-alcoholic fatty liver disease, visceral obesity, and TPO/GC ratio can accurately predict faster COX-1 recovery and suboptimal aspirin response. The TPO/GC ratio may serve as a simple tool to identify patients who would benefit from more frequent aspirin dosing.
Article
Biochemistry & Molecular Biology
Beatrice Dufrusine, Silvia Valentinuzzi, Sandra Bibbo, Verena Damiani, Paola Lanuti, Damiana Pieragostino, Piero Del Boccio, Ersilia D'Alessandro, Alberto Rabottini, Alessandro Berghella, Nerino Allocati, Katia Falasca, Claudio Ucciferri, Francesco Mucedola, Marco Di Perna, Laura Martino, Jacopo Vecchiet, Vincenzo De Laurenzi, Enrico Dainese
Summary: This study identified significant changes in proteins involved in iron metabolism in COVID-19 patients and long-COVID patients. The activation of 5-LOX was also observed, possibly through an iron-dependent mechanism. Leukotriene B4 and lipocalin 2 were suggested as potential markers for COVID-19 and long-COVID.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Simone Vespa, Pietro Del Biondo, Pasquale Simeone, Enrico Cavallucci, Giulia Catitti, Raffaella Auciello, Domenico De Bellis, Isotta Altomare, Laura Pierdomenico, Barbara Canonico, Ilaria Cicalini, Ilaria Angilletta, Piero Del Boccio, Damiana Pieragostino, Francesca Santilli, Andrea Urbani, Vincenzo De Laurenzi, Liborio Stuppia, Paola Lanuti
Summary: Allergic reactions to COVID-19 vaccine components are rare but should be considered. Skin tests combined with the basophil activation test (BAT) using PEG2000, PEG4000, and DMG-PEG2000 can help evaluate allergic reactions to the vaccine. The study suggests that even when the BAT is negative, a high stimulation index (SI) in basophil activation may indicate a low-grade reaction to PEG.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Giulia Catitti, Maria Concetta Cufaro, Domenico De Bellis, Ilaria Cicalini, Simone Vespa, Federico Tonelli, Giulia Miscia, Lorenzo Secondi, Pasquale Simeone, Vincenzo De Laurenzi, Damiana Pieragostino, Piero Del Boccio, Paola Lanuti
Summary: Platelet-derived extracellular vesicles (EVs) may play a significant role in tissue repair and contribute to the regeneration potential of platelet-rich plasma (PRP) preparations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Ilaria Cicalini, Samanta Moffa, Maria Lucia Tommolini, Silvia Valentinuzzi, Mirco Zucchelli, Ines Bucci, Piero Chiacchiaretta, Antonella Fontana, Luca Federici, Vincenzo De Laurenzi, Piero Del Boccio, Claudia Rossi, Damiana Pieragostino
Summary: This study developed a questionnaire to investigate the eating habits of 109 women during pregnancy and statistically correlated the results with the data obtained by the newborn screening laboratory. The results showed that maternal lifestyle, diet, and drug intake during pregnancy may affect the neonatal metabolic profile, potentially leading to false positive or false negative results in the newborn screening test. The findings highlight the importance of understanding maternal nutrition and lifestyle in preventing misinterpretations of the neonatal metabolic profile, reducing unnecessary stress for newborns and their parents, and limiting costs for the healthcare system.
Article
Oncology
Simone De Fabritiis, Silvia Valentinuzzi, Gianluca Piras, Ilaria Cicalini, Damiana Pieragostino, Sara Pagotto, Silvia Perconti, Mirco Zucchelli, Alberto Schena, Elisa Taschin, Gloria Simona Bertesteanu, Diana Liberata Esposito, Antonio Stigliano, Vincenzo De Laurenzi, Francesca Schiavi, Mario Sanna, Piero Del Boccio, Fabio Verginelli, Renato Mariani-Costantini
Summary: Head and neck paragangliomas (HNPGLs) are rare chemoresistant tumors curable only with surgery and are strongly influenced by genetic predisposition. Patients and relatives require lifetime follow-up with MRI and/or PET-CT, resulting in exposure to electromagnetic/ionizing radiation, costs, and organizational challenges.
Article
Medicine, General & Internal
Verena Damiani, Erika Pizzinato, Ilaria Cicalini, Gianmaria Demattia, Mirco Zucchelli, Luca Natale, Claudia Palmarini, Claudia Di Marzio, Luca Federici, Vincenzo De Laurenzi, Damiana Pieragostino
Summary: The aim of this study was to improve the detection of SARS-CoV-2 nucleocapsid antibodies by developing a simple and reliable DELFIA immunoassay on dried blood spots (DBSs). The results showed that the DBS-DELFIA had a wider dynamic range and higher sensitivity for detecting SARS-CoV-2 nucleocapsid antibodies. Furthermore, a strong correlation was found between the DBS-DELFIA and ELISA immunoassays. Therefore, the combination of dried blood sampling with DELFIA technology provides an easier, minimally invasive, and accurate measurement of SARS-CoV-2 nucleocapsid antibodies in previously infected individuals.