Article
Biochemistry & Molecular Biology
Stefania Mitola, Cosetta Ravelli, Michela Corsini, Alessandra Gianoncelli, Federico Galvagni, Kurt Ballmer-Hofer, Marco Presta, Elisabetta Grillo
Summary: In this study, we propose a multi-step approach for the expression and purification of histidine-tagged recombinant dimeric Gremlin-1 protein, which can act as a BMP antagonist and a VEGFR2 agonist, using HEK293T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
A. Young J. Park, Dat Q. Tran, Justin B. Schaal, Mengxi Wang, Michael E. Selsted, Paul M. Beringer
Summary: This study characterized the preclinical pharmacokinetics and safety of intravenous RTD-1. The results showed extensive tissue distribution and excellent safety profile for RTD-1, supporting its clinical investigation for the treatment of COVID-19 or other pulmonary inflammatory diseases.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Joao H. C. Manfrao-Netto, Enzo Bento Queiroz, Ana C. de Oliveira Junqueira, Antonio M. V. Gomes, Daniel Gusmao de Morais, Hugo Costa Paes, Nadia Skorupa Parachin
Summary: Hyaluronic acid, a biopolymer valued at USD 8.9 billion, is traditionally obtained from animal tissues and fermentative cultures. Efforts to engineer safe micro-organisms for HA synthesis have not yet yielded results as efficient as natural producers, prompting continued research in the field.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agronomy
Kai Zhao, Rui Ren, Xingli Ma, Kunkun Zhao, Chengxin Qu, Di Cao, Qian Ma, Yingying Ma, Fangping Gong, Zhongfeng Li, Xingguo Zhang, Dongmei Yin
Summary: This study reveals the role of defensin genes (AhDef) in peanut resistance against bacterial wilt caused by Ralstonia solanacearum. The AhDef genes show conserved architectural features and are associated with phytohormone signaling and defense responses. In particular, the AhDef2.2 gene enhances peanut resistance to R. solanacearum, suggesting its potential value in peanut breeding for disease resistance.
Review
Biochemical Research Methods
Anirban Banerjee, Valerie Ward
Summary: Microalgae is a promising platform for producing therapeutic recombinant proteins, which can be used as edible vaccines or drug delivery vehicles, in addition to their natural abundance as nutraceutical products.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinjun Chen, Zihao Liu, Yisong Liu, Xuewen Zhang, Jianguo Zeng
Summary: The study constructed a non-normalized cDNA library of Macleaya cordata to investigate its pathogenesis-related (PR) proteins, revealing rich active proteins, especially defensins, against pathogens. Recombinant expression of two defensins in yeast and exploration of their antimicrobial activities further confirmed their broad spectrum of antimicrobial properties. This study provides novel insights into the effective components of M. cordata and highlights the importance of PR proteins in its antibacterial and anti-inflammatory activities.
Article
Plant Sciences
Oranicha Hanittinan, Kaewta Rattanapisit, Ashwini Malla, Kittipan Tharakhet, Chutitorn Ketloy, Eakachai Prompetchara, Waranyoo Phoolcharoen
Summary: This study successfully utilized a plant expression system to produce anti-human IgE monoclonal antibodies with comparable functionality to commercial Omalizumab. Although further optimization is needed for antibody yield and purification process, the results demonstrate the promising potential of plant expression system as an effective method for Omalizumab production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Fan Yang, Zhangjun Cao, Can Li, Lin Chen, Guochao Wu, Xingping Zhou, Feng F. Hong
Summary: This study aimed to improve the industrial production of bacterial cellulose (BC) by genetically engineering Komagataeibacter xylinus to efficiently use mannose-rich biomass as a carbon source. Results showed that the recombinant strain cultured in mannose media had an 84% increase in BC yield and improved tensile strength and elongation compared to the wild-type strain. This strategy of modifying the mannose catabolic pathway has significant potential in reducing the production costs of BC from mannose-rich biomass.
Article
Microbiology
Gema Lozano Terol, Julia Gallego-Jara, Rosa Alba Sola Martinez, Adrian Martinez Vivancos, Manuel Canovas Diaz, Teresa de Diego Puente
Summary: The research evaluated the expression of YFP reporter protein under the control of different strength promoters and replication origins, with different E. coli strains and carbon sources. It was found that metabolic burden leads to decreased expression of recombinant proteins, highlighting the importance of balancing plasmid copy number and promoter strength for optimal expression. The results represent a significant advance in determining the most suitable expression system for improving both the quantity and quality of recombinant proteins in bioproduction engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mansour A. Dughbaj, Jordanna G. Jayne, A. Young J. Park, Timothy J. Bensman, Marquerita Algorri, Andre J. Ouellette, Michael E. Selsted, Paul M. Beringer
Summary: RTD-1 exhibited potent anti-inflammatory activity in a murine model of chronic P. aeruginosa lung infection, reducing lung white blood cell counts and proinflammatory gene expression, particularly inflammasome-related genes. This immunomodulatory peptide may be a promising therapeutic for CF-associated lung disease.
Article
Biochemistry & Molecular Biology
Paulina Diaz-Garrido, Rosa Elena Cardenas-Guerra, Ignacio Martinez, Sebastian Poggio, Karla Rodriguez-Hernandez, Lucio Rivera-Santiago, Jaime Ortega-Lopez, Sergio Sanchez-Esquivel, Bertha Espinoza
Summary: This study analyzed the trypanocidal activity of a type 1 recombinant defensin (rDef1.3) identified in a specific species from Mexico against various parasites. The results showed that rDef1.3 has important antimicrobial effects against Trypanosoma cruzi and Leishmania species, particularly Leishmania major, suggesting its potential as a treatment for neglected tropical diseases transmitted by insect vectors.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bikash K. Bhandari, Chun Shen Lim, Paul P. Gardner
Summary: Experiments planned using accurate prediction algorithms can reduce failures in recombinant protein production. TISIGNER offers three web services specialized in synonymous optimization, solubility, and signal peptide analysis, which are interconnected for users to switch between when optimizing genes of interest.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell Biology
Prasad Tongaonkar, Katie K. Trinh, Andre J. Ouellette, Michael E. Selsted
Summary: Theta- (theta-) defensins are host defense peptides with antimicrobial and immune-modulating activities. Rhesus theta-defensin-1 (RTD-1) inhibits proinflammatory gene expression and cytokine secretion by suppressing the NF-kappa B and MAP kinase pathways in immune-stimulated cells. RTD-1 also suppresses endotoxin tolerance and enhances TNF-alpha secretion and NF-kappa B activity upon secondary LPS stimulation by downregulating miR-146a expression and stabilizing IRAK1 protein. These findings reveal a novel role for RTD-1 in the innate immune response and its potential as an anti-inflammatory agent.
MEDIATORS OF INFLAMMATION
(2023)
Article
Biotechnology & Applied Microbiology
Oliver Goldbeck, Dominique N. Desef, Kirill V. Ovchinnikov, Fernando Perez-Garcia, Jens Christmann, Peter Sinner, Peter Crauwels, Dominik Weixler, Peng Cao, Judith Becker, Michael Kohlstedt, Julian Kager, Bernhard J. Eikmanns, Gerd M. Seibold, Christoph Herwig, Christoph Wittmann, Nadav S. Bar, Dzung B. Diep, Christian U. Riedel
Summary: Bacteriocins are antimicrobial peptides produced by bacteria that have emerged as commercial food preservatives and potential alternatives to antibiotics. Shifting production to recombinant microorganisms can improve production efficiency and product purity. Corynebacterium glutamicum has been identified as a suitable host for producing bacteriocin pediocin PA-1.
METABOLIC ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Nathalia Mayumi Noda-Nicolau, Mariana de Castro Silva, Giovana Fernanda Cosi Bento, Jeniffer Sena Baptista Ferreira, Juliano Novak, Julia Andrade Pessoa Morales, Julia Abbade Tronco, Aline Nascimento Bolpetti, Gabriel Vitor Silva Pinto, Jossimara Polettini, Camila Marconi, Marcia Guimaraes da Silva
Summary: This study found lower levels of hBDs1-3 in cervicovaginal fluids of women with bacterial vaginosis compared to those with normal microbiota. Lower levels of hBDs1-3 were independently associated with bacterial vaginosis.
Review
Biochemical Research Methods
Alexander Shekhtman, Ravichandran Ramasamy, Ann Marie Schmidt
EXPERT REVIEW OF PROTEOMICS
(2017)
Article
Biochemistry & Molecular Biology
Tao Bi, Yilong Li, Alexander Shekhtman, Julio A. Camarero
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Multidisciplinary
Subhabrata Majumder, Michael T. Jones, Michael Kimmel, Arun Alphonse Ignatius
PHARMACEUTICAL RESEARCH
(2018)
Review
Biochemistry & Molecular Biology
Dipankar Chaudhuri, Teshome Aboye, Julio A. Camarero
BIOCHEMICAL JOURNAL
(2019)
Article
Chemistry, Medicinal
Subhabrata Majumder, Wei Wang, Arun Alphonse Ignatius
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Corina Sarmiento, Julio A. Camarero
CURRENT PROTEIN & PEPTIDE SCIENCE
(2019)
Article
Chemistry, Multidisciplinary
Subhabrata Majumder, Andrew Saati, Shibu Philip, Lucy L. Liu, Elaine Stephens, Jason C. Rouse, Arun Alphonse Ignatius
PHARMACEUTICAL RESEARCH
(2019)
Article
Chemistry, Multidisciplinary
Rajasekaran Ganesan, Mansour A. Dughbaj, Lisa Ramirez, Steven Beringer, Teshome L. Aboye, Alexander Shekhtman, Paul M. Beringer, Julio A. Camarero
Summary: This study reports the development of a novel engineered cyclotide with effective broad-spectrum antibacterial activity against several ESKAPE bacterial strains and clinical isolates. The most active antibacterial cyclotide was extremely stable in serum, showed little hemolytic activity, and provided protection in vivo in a murine model of P. aeruginosa peritonitis. These results highlight the potential of the cyclotide scaffold for the development of novel antimicrobial therapeutic leads for the treatment of bacteremia.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Organic
Dipankar Chaudhuri, Rajasekaran Ganesan, Alicia Vogelaar, Mansour A. Dughbaj, Paul M. Beringer, Julio A. Camarero
Summary: A new epimerization-free approach for the Fmoc-based synthesis of murepavadin using intramolecular native chemical ligation and desulfurization reaction has been developed, resulting in a high yield of bioactive murepavadin in two steps. The synthetic peptide antibiotic showed potent activity against different clinical isolates of P. aeruginosa. This approach can be easily adapted for the production of murepavadin analogues and other backbone-cyclized peptides.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Subhabrata Majumder, Deep S. Bhattacharya, Alex Langford, Arun Alphonse Ignatius
Summary: In this study, the viscosity behavior of a set of Pfizer mAbs was correlated using Dynamic Light Scattering (DLS) and 2D NMR based methyl fingerprinting. Methyl fingerprinting allowed for differentiation of viscosity prone mAbs from well behaved ones even at high concentrations. The study provides important insights for stability, processing, and drug product development of therapeutic monoclonal antibodies.
PHARMACEUTICAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Rachayita Nag, Srishti Joshi, Anurag Singh Rathore, Subhabrata Majumder
Summary: In this study, the activity of L-asparaginase II (MW 135 kDa) from E. coli was observed using NMR technology. It was found that a flexible loop structure of the enzyme played a key role in its activity. Combining isothermal titration calorimetry (ITC) and NMR, it was demonstrated that changes in protein conformation could result in loss of function. The results indicate that 2D NMR can be reliably used to evaluate the structure-function relationship of this enzyme without the need for protein labeling.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biochemistry & Molecular Biology
Julio A. Camarero, Maria Jose Campbell
Review
Chemistry, Medicinal
Julio A. Camarero
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Biochemistry & Molecular Biology
Christopher M. DeMott, Subhabrata Majumder, David S. Burz, Sergey Reverdatto, Alexander Shekhtman