Article
Oncology
Xisha Chen, Kuansong Wang, Shilong Jiang, Hongyin Sun, Xuanling Che, Minghui Zhang, Jiaying He, Ying Wen, Mengting Liao, Xiangling Li, Xiaoming Zhou, Jianxun Song, Xingcong Ren, Wenjun Yi, Jinming Yang, Xiang Chen, Mingzhu Yin, Yan Cheng
Summary: Our study reveals that eEF2K is correlated with therapeutic response and prognosis in melanoma patients treated with anti-PD-1 therapy. We also show that eEF2K plays a vital role in regulating the tumor immune microenvironment by controlling PD-L1 expression. In addition, combining eEF2K inhibition with ICB therapy may provide a potential combination therapeutic strategy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Cell Biology
Yanan Cao, Qing Ye, Murong Ma, Qing-Bai She
Summary: Increased PD-L1 expression in cancer cells is achieved through internal ribosomal entry site (IRES)-mediated translation upon mTORC1 inhibition. The IRES element in the PD-L1 50-UTR enables cap-independent translation and continuous production of PD-L1 protein despite mTORC1 inhibition. The key protein eIF4A enhances PD-L1 IRES activity and protein production in tumor cells treated with mTOR kinase inhibitors (mTORkis).
Article
Food Science & Technology
Rosario Iglesias, Rosita Russo, Nicola Landi, Mariangela Valletta, Angela Chambery, Antimo Di Maro, Andrea Bolognesi, Jose M. Ferreras, Lucia Citores
Summary: This study isolated and characterized several RIPs and lectins from elderberry leaves, studying their properties and biological activities. The different tissues of elderberry contain a variety of RIPs and lectins, which are of great value in diagnostics, experimental therapy, and agriculture.
Article
Immunology
Lijun Wu, Shiyi Cai, Yiyun Deng, Zhe Zhang, Xiehai Zhou, Yong Su, Dujuan Xu
Summary: The PD-1/PD-L1 axis enhances cisplatin resistance in gastric cancer through PI3K/AKT-mediated P-gp expression.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Chemistry, Medicinal
Yangyang Meng, Cuiping Chu, Xinyu Niu, Liuyang Cheng, Di Wu, Lei Liu, Shaopeng Zhang, Tianqi Li, Yunlei Hou, Yajing Liu, Mingze Qin
Summary: In this study, a novel series of 4-phenylindolines containing a (5-cyanopyridin-3-yl)methoxy moiety were developed, and compounds M17 and M23 exhibited the most potent ability to disrupt the PD-1/PD-L1 interaction, making them promising lead compounds for developing potent inhibitors of the PD-1/PD-L1 axis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Inorganic & Nuclear
Mohammad Ameskal, Rachid Taakili, Ekaterina S. Gulyaeva, Carine Duhayon, Jeremy Willot, Noel Lugan, Christine Lepetit, Dmitry A. Valyaev, Yves Canac
Summary: An efficient synthesis of stable Pd(II) complexes with diverse coordination frameworks is developed. The complex bearing a nonsymmetrical NHC core pincer scaffold shows competitive donor properties. The bonding situation of C- and P-bound phosphonium ylide fragments within the same molecule is studied for the first time.
INORGANIC CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Xiang-Lei Yan, Qiu-Yun Luo, Su-Na Zhou, Wen-Tao Pan, Lin Zhang, Da-Jun Yang, Miao-Zhen Qiu
Summary: miR-375 negatively regulates PD-L1 expression in gastric cancer via the JAK2/STAT3 signaling pathway, showing potential as a promising new therapeutic target in the future treatment of gastric cancer.
CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY
(2021)
Article
Cell Biology
Rui Zhang, Ziqi Meng, Xuwei Wu, Meihua Zhang, Zhengri Piao, Tiefeng Jin
Summary: PD-L1 plays an important role in non-small cell lung cancer and can reverse the malignant progression of tumor cells by inhibiting TAM/M2 polarization.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Oncology
Yuting Zhang, Xianyong Chen, Hongmei Zheng, Yuting Zhan, Jiadi Luo, Yang Yang, Yue Ning, Haihua Wang, Weiyuan Wang, Songqing Fan
Summary: The study revealed elevated expression levels of PD-1, PD-L1, and p-S6 proteins in nasopharyngeal carcinoma tissues, with significantly increased co-expression of PD-1 and PD-L1. Patients with positive expression of PD-L1 showed a significantly higher overall survival rate, while those with positive expression of PD-1 and p-S6 had significantly lower rates. Co-expression of PD-1 and PD-L1 was associated with lower overall survival rate.
Article
Oncology
Junling Ren, Xiao Han, Hannah Lohner, Rosalie G. Hoyle, Jiong Li, Shuang Liang, Huizhi Wang
Summary: New research reveals that Porphyromonas gingivalis (Pg) infection enhances PD-L1 expression on dendritic cells (DCs), suppresses CD8+ T-cell cytotoxicity, and exacerbates oral cancer growth. This suggests that targeting Pg and/or its mediated signaling could potentially improve checkpoint blockade immunotherapy.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Oncology
Daisuke Yoneta, Junji Kato, Takafumi Kamiya, Kohei Horimoto, Sayuri Sato, Masahide Sawada, Tomoyuki Minowa, Tokimasa Hida, Shintaro Sugita, Hisashi Uhara
Summary: In this study, the differences in PD-L1 expression in melanoma tissues among commonly used PD-L1 antibodies were investigated. The positive rates of three antibodies (E1L3N, SP142, and 28-8) were within the acceptable range, with E1L3N and SP142 showing slightly higher rates than 28-8. Additionally, CD4+ TILs and CD8+ TILs were quantitatively correlated with PD-L1-positive tumor cells.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Oncology
Mohsin Shah, Ronac Mamtani, Melina E. Marmarelis, Sean Hennessy
Summary: In this cohort study, chemoimmunotherapy did not show an overall benefit over immunotherapy alone in the first-line treatment of advanced non-small cell lung cancer (aNSCLC). However, it did provide an early survival advantage in both the overall cohort and the subgroup of patients with a high PD-L1 expression (>90%). Future studies should focus on identifying higher risk patients who may benefit from the addition of chemotherapy.
CLINICAL LUNG CANCER
(2023)
Article
Respiratory System
Xianfeng Chen, Juntao Hu, Jie Lai, Zhiyong Zhang, Zhanhong Tang
Summary: Dexmedetomidine (DEX), a novel alpha-2 adrenoceptor agonist, exhibits anti-inflammatory properties. This study found that DEX treatment increased miR-140-3p expression, leading to the inhibition of PD-L1 expression and the alleviation of inflammation and alveolar type II cell injury.
CANADIAN RESPIRATORY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Xuanzhen Chen, Meng Wang, Xiaowei Wang, Junyi Liu, Zhili Zhang, Chao Tian
Summary: In this study, a series of 2-[p-fluorophenyl]-6-substituted-9H-purine analogues were synthesized and designed as CHK1 inhibitors. Compound b22 showed significant antitumor potentiation on HT29 cells and reversed the upregulation of PD-L1 induced by Gem.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Chemistry, Medicinal
Jeffrey Yang, Subhadwip Basu, Longqin Hu
Summary: Small-molecule inhibitors targeting the PD-1/PD-L1 immune checkpoint axis are being explored as potential alternative therapies. In this study, a series of new derivatives were designed and evaluated for their inhibitory activity against the PD-1/PD-L1 protein-protein interaction. The inhibitors with specific appendages showed improved activity.
MEDICINAL CHEMISTRY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Flavia Squeglia, Daniela Marasco, Alessia Ruggiero, Genni Testa, Luciana Esposito, Rita Berisio
Summary: This study explores the suitability of RipA, a key cell division protein of Mycobacterium tuberculosis, for whole cell biosensing of Gram-positive bacteria. The research findings show the potential of the engineered RipA molecule to be integrated in the development of early warning biosensors for Gram-positive contamination in clinical diagnosis or food-borne infections.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Paola Fortugno, Rosanna Monetta, Valeria Cinquina, Chiara Rigon, Francesca Boaretto, Chiara De Luca, Nicoletta Zoppi, Luana Di Leandro, Emanuela De Domenico, Arianna Di Daniele, Rodolfo Ippoliti, Francesco Angelucci, Ernesto Di Cesare, Ruggero De Paulis, Leonardo Salviati, Marina Colombi, Francesco Brancati, Marco Ritelli
Summary: Pathogenic variants in TGFBR1 are a common cause of Loeys-Dietz syndrome (LDS) characterized by life-threatening cardiovascular diseases. In this study, two novel variants in TGFBR1 were identified in LDS patients, resulting in truncated TGFBR1 proteins. These variants escaped nonsense-mediated mRNA decay and exhibited enhanced TGF beta signaling. The findings emphasize the importance of functional studies for accurate clinical diagnosis.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Chemistry, Applied
Nicola Landi, Maja Grundner, Sara Ragucci, Miha Pavsic, Martina Mravinec, Paolo Pedone, Kristina Sepcic, Antimo Di Maro
Summary: This study reports the characterization and cytotoxic effects of four novel ribotoxin-like proteins (RL-Ps), called eryngitins, found in the king oyster mushroom. These proteins show enzymatic activity and exhibit cytotoxic action against insect cells, suggesting their potential use as bioinsecticides in biotechnological applications.
Article
Food Science & Technology
Nicola Landi, Vincenza Ciaramella, Sara Ragucci, Angela Chambery, Fortunato Ciardiello, Paolo V. Pedone, Teresa Troiani, Antimo Di Maro
Summary: This study aimed to improve the therapeutic action of cetuximab by conjugating it with the RIP quinoin isolated from quinoa seeds. The novel quinoin cetuximab immunoconjugate showed significant dose-dependent cytotoxicity against cetuximab-resistant cells, suggesting its potential as a candidate for targeted therapy in the treatment of cetuximab-resistant metastatic colorectal cancer.
Review
Cell Biology
Maria Romano, Flavia Squeglia, Eliza Kramarska, Giovanni Barra, Han-Gyu Choi, Hwa-Jung Kim, Alessia Ruggiero, Rita Berisio
Summary: Tuberculosis remains the leading infectious bacterial cause of death worldwide. Developing a new vaccine with better coverage than the current BCG vaccine is vital to stop its spread. Structural Vaccinology and the development of vaccine adjuvants can enhance the immunostimulating effects of subunit vaccines, accelerating the development of a safer and more effective tuberculosis vaccine.
Article
Biochemistry & Molecular Biology
Sara Ragucci, Hafiza Zumra Fatima Hussain, Andrea Bosso, Nicola Landi, Angela Clemente, Paolo Vincenzo Pedone, Elio Pizzo, Antimo Di Maro
Summary: White button mushroom is a widely consumed edible mushroom with rich nutrients and bioactive molecules. A novel ribotoxin-like protein (RL-P) with specific ribonucleolytic activity has been purified and characterized from this mushroom, showing lower thermal stability compared to RL-Ps from other mushrooms.
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Alessia Ruggiero, Emanuela Iaccarino, Angela Oliver, Flavia Squeglia, Miguel Moreira, Luciana Esposito, Menotti Ruvo, Rita Berisio
Summary: CD59 is an abundant protein in humans that protects cells from damage by inhibiting the complement system. It inhibits the assembly of the Membrane Attack Complex (MAC) and is incorporated by pathogenic viruses like HIV-1 to escape complement-mediated virolysis. CD59 is also overexpressed in cancer cells to resist complement attack. In this study, bioinformatics and computational tools were used to identify CD59 interactions and design paratope-mimicking peptides that target CD59.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucia Citores, Sara Ragucci, Rosita Russo, Claudia C. Gay, Angela Chambery, Antimo Di Maro, Rosario Iglesias, Jose M. Ferreras
Summary: We have isolated and purified a cytotoxic protein called Ledodin from shiitake mushrooms, which consists of a 197 amino acid chain. Ledodin showed N-glycosylase activity on mammalian 28S rRNA and inhibited protein synthesis, but had no effect on insect, fungal, and bacterial ribosomes. It is believed to have a catalytic mechanism similar to that of DNA glycosylases and plant ribosome-inactivating proteins. Ledodin could be the first representative of a new family of enzymes widely distributed among basidiomycetes. These proteins are of interest due to their potential toxicity in edible mushrooms and their applications in medicine and biotechnology.
Article
Biochemistry & Molecular Biology
Eliza Kramarska, Flavio De Maio, Giovanni Delogu, Rita Berisio
Summary: This study used AlphaFold2.0 computations to model the polymorphic forms of the PE_PGRS33 protein. Through sequence distribution, phylogenetic and antigenic prediction analyses, the structural impact of the observed mutations was predicted. The results correlated well with the observed frequency and phenotypic features, and protein variants associated with bacterial evolution were identified.
Article
Biology
Giovanni Smaldone, Giovanni Pecoraro, Katia Pane, Monica Franzese, Alessia Ruggiero, Luigi Vitagliano, Marco Salvatore
Summary: The KCTD protein family has been traditionally recognized as playing important roles in neurological physiopathology, but recent studies have shown their involvement in various biological processes, including cancers. This study focused on KCTD1 and its role in colorectal cancer. Knocking down KCTD1 in SW480 cells led to significant effects on their motility and stemness, indicating an oncosuppressor role for this protein. Similar effects were also observed with KCTD12 misregulation. These results expand the understanding of KCTD1's involvement in different physiological and pathological processes and suggest analogous activities among KCTD protein family members.
Article
Food Science & Technology
Mariangela Valletta, Nicoletta Campolattano, Ida De Chiara, Rosangela Marasco, Vikram Pratap Singh, Lidia Muscariello, Paolo Vincenzo Pedone, Angela Chambery, Rosita Russo
Summary: Due to the increasing consumer attention to functional foods with probiotics, the market for fermented milk beverage Kefir is growing. There is a demand for new methods to certify the authenticity of Kefir products. In this study, an innovative nanoLC-ESI-MS/MS-based methodology was developed to profile lactic acid bacteria in Kefir products and grains at different levels. This methodology enables the authentication of probiotic starters and provides insights into the composition of lactic acid bacteria in unlabelled Kefir, highlighting the potential of LC-MS/MS as a sensitive tool for assessing the authenticity of fermented foods.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Sara Ragucci, Stefany Castaldi, Nicola Landi, Rachele Isticato, Antimo Di Maro
Summary: Ageritin from poplar mushrooms is an antifungal enzyme that inhibits the growth of various fungi affecting crops. It specifically hydrolyzes a phosphodiester bond in the large rRNA, blocking protein synthesis. The antifungal activity of ageritin was demonstrated against Botrytis cinerea, Colletotrichum truncatum, and Alternaria alternata, while no effect was observed on Trichoderma harzianum. In vitro studies revealed that ageritin can damage fungal ribosomes, and further experiments in liquid medium confirmed its concentration-dependent antifungal effect on B. cinerea and T. harzianum.
Article
Food Science & Technology
Nicola Landi, Laura Alberico, Angela Clemente, Stefania Peddio, Hafiza. Z. F. Hussain, Sara Ragucci, Paolo Zucca, Pasqualina Woodrow, Antimo Di Maro
Summary: Italy is rich in uncharacterized common bean landraces. This study evaluated the composition and profiles of 'Cerato' and 'Curniciello' dry beans cultivated in Caserta, Southern Italy, and compared them with other local dry beans. 'Cerato' beans had lower levels of proteins, lipids, and amino acids compared to 'Curniciello' beans. Fatty acid analysis showed that linoleic acid, alpha-linolenic acid, and oleic acid were the most abundant in both beans. The inhibitory activities of enzymes related to protein and carbohydrate intake were also investigated. AFLP analysis provided useful information for authentication and traceability. Overall, this study contributes to the preservation of local culinary traditions and Italian biodiversity.
Article
Microbiology
Michela Di Napoli, Brigida Silvestri, Giusy Castagliuolo, Andrea Carpentieri, Giuseppina Luciani, Antimo Di Maro, Sergio Sorbo, Alessandro Pezzella, Anna Zanfardino, Mario Varcamonti
Summary: In this study, a fungal species, Cladosporium halotolerans, is isolated from Galleria mellonella larvae. It is found that this fungus is capable of interacting with and degrading polyethylene.
FEMS MICROBIOLOGY ECOLOGY
(2023)