Article
Neurosciences
Khris G. Griffis, Katherine E. Fehlhaber, Fred Rieke, Alapakkam P. Sampath
Summary: The sensitivity of retinal cells is altered in response to background light. The sensitivity adjustments in rods and postsynaptic modulation of the transduction cascade in rod bipolar cells are involved in this adaptation. The background illumination affects both rod and rod bipolar cell sensitivity.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Engineering, Industrial
Kaige Su, Jianhua Liu, Hui Xiong
Summary: Bearing fault diagnosis is crucial for the operation of mechanical equipment. Traditional deep-learning methods perform well when training and testing samples have similar distributions, but they fail to adapt to changing conditions. To address this, a new branch multi-level adaptation model based on convolutional neural networks is proposed, enabling hierarchical diagnosis of bearing faults and reducing distributional distances among multiple levels. Experimental results show its robust adaptability and superior performance compared to other methods.
JOURNAL OF MANUFACTURING SYSTEMS
(2022)
Article
Engineering, Multidisciplinary
Baokun Han, Xiao Zhang, Jinrui Wang, Zenghui An, Sixiang Jia, Guowei Zhang
Summary: This study introduces a novel transfer learning method called hybrid distance-guided adversarial network (HDAN) to address the issue of degradation in diagnostic accuracy caused by data shift in intelligent fault diagnosis. Experimental results demonstrate that HDAN can achieve better feature cluster performance of the same class in different domains.
Article
Biochemical Research Methods
Min Yan, Mei X. Wu
Summary: This study demonstrates that pre-treatment with low-level light therapy can significantly enhance myotube formation and myoblast differentiation by increasing mitochondrial mass, myogenin expression, and reducing lactate production under hypoxic conditions, providing valuable insights for the timing and potential effects of LLLT on muscle strength in conjunction with anaerobic exercise.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Engineering, Geological
Bo Li, Ruyi Bao, Ye Wang, Richeng Liu, Cheng Zhao
Summary: The numerical modeling study of the permeability evolution of fracture networks during shearing under specific boundary conditions reveals a three-stage behavior, influenced by factors such as confining pressure, shear displacement, and fractal dimension. Hydraulic pressure tends to open fractures and enhance permeability, with a magnitude of enhancement similar to the increase in hydraulic pressure.
ROCK MECHANICS AND ROCK ENGINEERING
(2021)
Article
Engineering, Environmental
Shanmuganathan Venkatesan, Tzu-Hsien Hsu, Xin-Wen Wong, Hsisheng Teng, Yuh-Lang Lee
Summary: By using transparent photoelectrodes and counter electrodes in tandem DSSCs, and regulating the film thicknesses of the top and bottom cells, both light harvest and charge transfer performance can be improved simultaneously, leading to an increased conversion efficiency of the solar cells.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Tianfu Li, Zhibin Zhao, Chuang Sun, Ruqiang Yan, Xuefeng Chen
Summary: The study introduces a domain adversarial graph convolutional network (DAGCN) that has made significant progress in mechanical fault diagnosis, modeling class labels, domain labels, and data structures to achieve unsupervised domain adaptation.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Engineering, Chemical
Georges Bastin, Veronique Chotteau, Alain Vande Wouwer
Summary: This study fills a gap in the analysis of flux distribution in VERO cell metabolism by providing an analysis of metabolic networks under different culture conditions. The calculation of unique flux distributions based on known macromolecular composition gives insights into metabolic changes triggered by culture conditions, which could support the design of feedback control strategies in bioreactors.
Article
Neurosciences
Rikard Frederiksen, Ala Morshedian, Sonia A. Tripathy, Tongzhou Xu, Gabriel H. Travis, Gordon L. Fain, Alapakkam P. Sampath
Summary: Rod photoreceptors can become saturated by bright background light, but recent experiments have shown that they can also recover sensitivity and maintain responses even under prolonged exposure to bright light. The reduction of translocation of G protein transducin in transgenic mice may affect the recovery ability, and a novel mechanism of visual-pigment regeneration within the rod itself enables them to remain responsive. This allows rods to avoid extended closure of transduction channels, which could lead to photoreceptor degeneration.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Horticulture
Yawen He, Vivek Yadav, Shijian Bai, Jiuyun Wu, Xiaoming Zhou, Wen Zhang, Shouan Han, Min Wang, Bin Zeng, Xinyu Wu, Haixia Zhong, Fuchun Zhang
Summary: The evaluation and comparison of photosynthetic characteristics of five Chinese hybrid varieties revealed that 'Jing Hongbao' and 'Ruidu Cuixia' demonstrated better photosynthetic performance, indicating their adaptability to the climate conditions in Xinjiang.
Article
Chemistry, Analytical
Wangpeng He, Jing Chen, Yue Zhou, Xuan Liu, Binqiang Chen, Baolong Guo
Summary: In this paper, a novel fault diagnosis method combining a generative adversarial network and transfer learning is proposed to address the problems of insufficient data and data distribution variations under different working conditions. The method generates dummy samples and extracts transfer fault characteristics to improve the accuracy and applicability of the diagnostic model.
Article
Chemistry, Multidisciplinary
Younghwa Yoon, Ken-ichi Katsumata, Norihiro Suzuki, Kazuya Nakata, Chiaki Terashima, Kyung Hwan Kim, Akira Fujishima, Jeongsoo Hong
Summary: In this study, beta-phase iron oxyhydroxide (beta-FeOOH) was synthesized using a hydrothermal method, and its photofunctional properties were investigated. Hydrogen production was successfully achieved by irradiating ultraviolet light on beta-FeOOH.
Article
Thermodynamics
Kenichi Katono, Kiyoshi Fujimoto, Takahiro Arai, Shuichiro Miwa, Takashi Hibiki
Summary: This study investigated the void fraction distributions in 5 x 5 rod-bundle test sections and obtained 3D void fraction distributions using a newly developed measurement system. The existing correlations showed good prediction of void fraction at high mass flux conditions but poorer performance at low mass flux conditions in narrow rod test sections. Additionally, the study assessed the effect of rod pitch lengths on void fraction distribution.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2022)
Article
Automation & Control Systems
Tongtong Jin, Chuliang Yan, Chuanhai Chen, Zhaojun Yang, Hailong Tian, Jinyan Guo
Summary: This paper introduces a novel fault diagnosis method using deep learning models to learn from collected signals and addresses the issue of inconsistency in feature distribution between training and test datasets. Experimental results demonstrate the reliability and stability of the proposed method.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2023)
Article
Developmental Biology
Hannah Lufkin, Diana Flores, Zachary Raider, Manoj Madhavan, Madeline Dawson, Anna Coronel, Dhruv Sharma, Ripla Arora
Summary: The movement of pre-implantation embryos is vital for successful pregnancy, but the influence of ovarian hormones on embryo movement is not fully understood. In this study, two mouse models were used to investigate the effects of altered hormonal environment on embryo location. It was found that both progesterone (P4) and estrogen (E2) can modulate embryo movement, and understanding the natural hormonal adaptation in diapause could provide insights into implantation success in hormonally altered conditions. This knowledge has potential applications in improving pregnancy survival and implantation success in clinical settings.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Seher Abbas, Valerio Marino, Nicole Weisschuh, Sinja Kieninger, Maria Solaki, Daniele Dell'Orco, Karl-Wilhelm Koch
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Valerio Marino, Matteo Riva, Davide Zamboni, Karl-Wilhelm Koch, Daniele Dell'Orco
Summary: This study investigates the mechanism by which the calcium-sensor protein recoverin regulates the activity of rhodopsin kinase in photoreceptors. The presence of both GRK1 and membrane is crucial for the physiological response to changes in intracellular calcium levels, driving the structural transitions of recoverin via a conformational selection mechanism. These findings may have implications for other sensory transduction systems involving protein complexes and biological membranes.
Editorial Material
Biochemistry & Molecular Biology
Daniele Dell'Orco
Summary: The study investigated the interaction between calmodulin and peptides mimicking different structural regions of the cardiac ryanodine receptor at varying calcium concentrations. It identified the specific CaM domains that bind to the CaM-binding domain in RyR2, showing that the interaction occurs under Ca2+-saturating conditions. This methodology may have broad applicability to the study of Ca2+ signaling and diseases associated with mutations in CaM-encoding genes.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ulrich Kellner, Nicole Weisschuh, Silke Weinitz, Ghazaleh Farmand, Sebastian Deutsch, Friederike Kortum, Pascale Mazzola, Karin Schaferhoff, Valerio Marino, Daniele Dell'Orco
Summary: A novel missense variant in the C1QTNF5 gene (p.(Q180E)) was found to cause autosomal dominant gyrate atrophy-like choroidal dystrophy (adGALCD) with unique clinical features compared to late-onset retinal degeneration (LORD). Protein tertiary and quaternary structure destabilization due to the variant was proposed as the mechanism behind the specific phenotype observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Valerio Marino, Giuditta Dal Cortivo, Paolo Enrico Maltese, Giorgio Placidi, Elisa De Siena, Benedetto Falsini, Matteo Bertelli, Daniele Dell'Orco
Summary: This study presents a novel GCAP1 variant associated with cone dystrophy (COD), characterized by severe alterations in electroretinogram under both scotopic and photopic conditions. Biochemical and biophysical analysis revealed compromised Ca2+-sensitivity of the variant, leading to constitutive activation of guanylate cyclase at physiological Ca2+ levels. The dysregulation of the GCAP1-cyclase complex not only affects second messenger accumulation and cell death, but also disrupts synaptic communication with bipolar cells, ultimately altering the ON-pathway related to b-wave generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Giuditta Dal Cortivo, Carlo Giorgio Barracchia, Valerio Marino, Mariapina D'Onofrio, Daniele Dell'Orco
Summary: Mutations associated with lethal arrhythmia in Calmodulin alter protein-protein interaction with Ryanodine receptor 2. Mutants show common alterations in recognition and faster dissociation rate than wild-type CaM. CaM's N-terminal lobe interacts with the target through allosteric interactions initiated by Ca2+.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nicole Weisschuh, Valerio Marino, Karin Schaeferhoff, Paul Richter, Joohyun Park, Tobias B. Haack, Daniele Dell'Orco
Summary: Exonic variants in disease-associated genes can have pathogenic effects by altering amino acid sequences or affecting pre-mRNA splicing. This study focused on analyzing nine exonic nucleotide changes in the GTPase-encoding domain of OPA1, a major gene underlying dominant optic atrophy. Different outcomes were observed for variants at codon 438, with some destabilizing dimerization and others causing exon skipping. The combination of RNA analysis and protein modeling proved valuable in assigning patients to precision therapies.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Amedeo Biasi, Valerio Marino, Giuditta Dal Cortivo, Paolo Enrico Maltese, Antonio Mattia Modarelli, Matteo Bertelli, Leonardo Colombo, Daniele Dell'Orco
Summary: This study identified a novel variant of GUCA1A causing an amino acid substitution in GCAP1, leading to increased affinity with GC and a doubled IC50 value, potentially resulting in hyper-activity of GC and toxic accumulation of cGMP and Ca2+, ultimately triggering cell death in photoreceptor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Paola Tesolin, Sofia Fiorino, Stefania Lenarduzzi, Elisa Rubinato, Elisabetta Cattaruzzi, Lydie Ammar, Veronica Castro, Eva Orzan, Claudio Granata, Daniele Dell'Orco, Anna Morgan, Giorgia Girotto
Summary: Pendred syndrome is characterized by sensorineural hearing loss, inner ear malformations, and goiter, primarily caused by mutations in the SLC26A4 gene. This study differentiated patient types through deep evaluation, with five patients carrying SLC26A4 variants showing characteristic audiograms and five others carrying different variants. In one patient negative for the SLC26A4 gene, a variant in the MYO5C gene was identified as a potential new candidate gene for hearing loss.
Article
Ophthalmology
Rosa Longo, Anna Avesani, Giulia Dalla Mura, Daniele Dell'Orco, Stefano Manfredini, Giacomo Panozzo
Summary: Treatment with U-HACL tear substitute resulted in significant improvement of ocular surface status in dry eye patients, as evidenced by improvements in symptoms, tear film parameters, and corneal/conjunctival damage scores.
EXPERT REVIEW OF OPHTHALMOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Giuditta Dal Cortivo, Daniele Dell'Orco
Summary: CIB2 is a protein capable of binding calcium and magnesium ions, playing an important role in cochlear hair cells and being associated with non-syndromic deafness. Besides its involvement in auditory physiology, CIB2 has diverse functions in various processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anna Avesani, Laura Bielefeld, Nicole Weisschuh, Valerio Marino, Pascale Mazzola, Katarina Stingl, Tobias B. Haack, Karl-Wilhelm Koch, Daniele Dell'Orco
Summary: This study characterizes the biochemical and biophysical properties of human GCAP3 and identifies a mutation in GUCA1C that may contribute to retinitis pigmentosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jan-Philipp Bodenbender, Valerio Marino, Leon Bethge, Katarina Stingl, Tobias B. B. Haack, Saskia Biskup, Susanne Kohl, Laura Kuehlewein, Daniele Dell'Orco, Nicole Weisschuh
Summary: This study analyzed clinical heterogeneity and characterized the underlying biallelic variants in TULP1 in 17 patients. The majority of patients exhibited severe rod-driven inherited retinal degeneration, while a fraction of the patients had cone-driven disease. The findings support the hypothesis that TULP1 variants lead to misfolding and trigger unfolded protein response, resulting in photoreceptor death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sabrina Asteriti, Valerio Marino, Anna Avesani, Amedeo Biasi, Giuditta Dal Cortivo, Lorenzo Cangiano, Daniele Dell'Orco
Summary: This study explored the possibility of modulating the phototransduction cascade in mouse rods using direct or liposome-mediated administration of a recombinant protein. The results showed that the exogenous protein was fully incorporated into the mouse retina and induced a disease-like electrophysiological phenotype in the presence of a specific mutation. These findings are important for the treatment of autosomal dominant genetic diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna Avesani, Valerio Marino, Serena Zanzoni, Karl-Wilhelm Koch, Daniele Dell'Orco
Summary: Human GCAP2 and its G157R variant exhibit differences in biochemical properties and may be associated with inherited retinal degeneration.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)