Article
Biochemistry & Molecular Biology
Miaohong Lin, Zhuoyan Dong, Hongkai Zhou, Guanyu Wu, Liang Xu, Sheng Ying, Miao Chen
Summary: The MYB gene family plays important roles in plant development and stress response. In this study, 208 PgMYB genes were identified in the pearl millet genome and analyzed for their phylogenetic relationships, gene duplications, and expression patterns. The results provide valuable insights into the molecular features of the MYB family in pearl millet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Madhu, Amandeep Kaur, Shivi Tyagi, Shumayla, Kashmir Singh, Santosh Kumar Upadhyay
Summary: A total of seven GR genes were identified in wheat, revealing their important role in abiotic stress response. Structural characterization and expression profiling provided insights into the characteristics and mechanisms of GR genes through phylogenetic analysis.
PLANT CELL REPORTS
(2022)
Article
Environmental Sciences
Senlin Yang, Jian Zhang, Lianghua Chen
Summary: Pennisetum sp. shows varying growth and physiological responses to different concentrations of Cd and different soil types. Higher Cd concentrations inhibit growth, decrease physiological and biochemical indices, disrupt antioxidative enzyme activity, and limit the production of soluble sugars and proteins.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rajendra Prasad Meena, Gourab Ghosh, Harinder Vishwakarma, Jasdeep Chatrath Padaria
Summary: This study functionally characterized the PgDREB2A gene in pearl millet and found that it can improve plant tolerance to abiotic stresses such as drought and salt stress. This research has potential application value for breeding stress-tolerant crops.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Plant Sciences
Kummari Divya, Sudhakar Reddy Palakolanu, Polavarapu Kavi Kishor, Aishwarya Shankhapal Rajesh, Vincent Vadez, Kiran K. Sharma, Pooja Bhatnagar Mathur
Summary: LEA genes in pearl millet respond differently to abiotic stresses, with PgLEA7 being significantly expressed under high VPD. The promoter regions of PgLEA contain stress-specific cis-acting elements, suggesting a role in abiotic stress tolerance in plants.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Environmental Sciences
Imran Khan, Samrah Afzal Awan, Muhammad Rizwan, Muhammad Adnan Akram, Muhammad Zia-ur-Rehman, Xiaosan Wang, Xinquan Zhang, Linkai Huang
Summary: This study investigated the effect of silver nanoparticles (AgNPs) on the transcriptome response of pearl millet under salinity. The results showed that the combined application of NaCl and AgNPs improved the morphology, physiology, biochemistry, and gene expression pattern of pearl millet, mitigating the negative effects of salt stress.
ENVIRONMENTAL POLLUTION
(2023)
Article
Plant Sciences
Shweta Jha, Jawahar Singh, Chaya Chouhan, Omvir Singh, Rakesh K. Srivastava
Summary: This study focused on dissecting the genetic diversity of 33 pearl millet genotypes for salinity tolerance through in vitro screening, revealing significant differences in growth characteristics among the genotypes. Four diverse genotypes were selected for further biochemical and molecular analysis based on their stress tolerance traits. Physiological studies confirmed that salt tolerance in certain genotypes may be attributed to higher osmolyte content and antioxidant enzyme activity. Genes such as catalase and nitrate reductase were found to play a major role in scavenging reactive oxygen species under salinity stress.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Genetics & Heredity
Jinhang Lv, Yue Xu, Xuming Dan, Yuchen Yang, Chunli Mao, Xixi Ma, Jie Zhu, Min Sun, Yarong Jin, Linkai Huang
Summary: MYB transcription factors play essential roles in plant defense mechanisms and stress responses, in addition to their involvement in developmental and metabolic processes. An analysis of six pearl millet genomes identified 1133 MYB genes, which formed four distinct phylogenetic subgroups. The duplication pattern of MYB genes in pearl millet genomes indicates their conserved evolutionary history. MYB genes were found to be primarily involved in drought and heat stress responses, particularly in root tissues. Through the modulation of abscisic acid-related pathways, circadian rhythms, and histone modification processes, MYB genes mediate abiotic stress responses. Duplicated MYB genes, including PMA5G04432.1 and PMA2G00728.1, exhibit consistent positive expression trends under various abiotic stresses, suggesting their crucial role in the adaptive responses of pearl millet.
Article
Biochemistry & Molecular Biology
Ming Sun, Shoujiang Sun, Zhicheng Jia, Wen Ma, Chunli Mao, Chengming Ou, Juan Wang, Han Zhang, Liu Hong, Manli Li, Shangang Jia, Peisheng Mao
Summary: Abiotic stress affects plant cellular redox homeostasis, leading to inhibition of seed germination and plant growth, which is a critical factor limiting crop yield. This study focuses on the molecular characterization, phylogenetic relationship, and RNA expression changes of glutathione reductase (GR) in Avena sativa (oat) under abiotic stress during seed imbibition. The results showed distinct expression patterns of GR genes and different responses to various stress treatments. Understanding the function of GRs could potentially improve oat stress tolerance and seed vigor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yingwei Qu, Shashi Kumar Gupta, Ambika Dudhate, Harshraj Subhash Shinde, Shenkui Liu, Tetsuo Takano, Daisuke Tsugama
Summary: In this study, 12 DOF family genes (PgDOFs) were identified in pearl millet, and most of them were upregulated under cold, heat, dehydration and salinity stress. These data are important for further characterization of the PgDOFs.
TROPICAL PLANT BIOLOGY
(2023)
Article
Plant Sciences
Yinli Bi, Zike Xue
Summary: The influence of dark septate endophytic (DSE) on the antioxidant activity of Astragalus membranaceus var. mongholicus under heat stress was investigated. DSE inoculation significantly decreased the malondialdehyde (MDA) content during the initial three days of heat stress. The activities of superoxide dismutase (SOD) and peroxidase (POD) of A. membranaceus leaves were significantly enhanced by DSE inoculation under heat stress, with SOD activities being 63-81% higher than in other treatments. The glutathione (GSH) and putrescine (Put) contents accumulated significantly on the third day under heat stress with DSE inoculation.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Horticulture
Shujuan Liu, Lei Yu, Lipan Liu, Aihong Yang, Xingyu Huang, Anfan Zhu, Hua Zhou
Summary: The effect of UV-B irradiation on the antioxidant capacity and growth of lettuce was investigated. UV-B exposure did not decrease lettuce weight but decreased photosynthetic performance initially. AsA, GSH, and H2O2 levels increased with UV-B exposure, and the expression of genes involving AsA recycling was upregulated.
Article
Evolutionary Biology
Faten Dhawi
Summary: This study used advanced next-generation RNA sequencing to investigate the gene expression profiles of pearl millet under heat stress. Through computational analysis, several important genes and signaling pathways related to thermal stress were identified. This research provides important clues for understanding the molecular mechanisms behind pearl millet's ability to withstand heat stress.
EVOLUTIONARY BIOINFORMATICS
(2023)
Article
Biology
Zainab M. Almutairi
Summary: This study isolated and sequenced the RL18/L5e gene from pearl millet, and found that its expression was significantly reduced under cold and drought stress, while highest under heat stress. These findings provide insights into the role of the RL18/L5e gene in adaptation to extreme environments.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shenglan Wang, Xinxin Sun, Xinyue Miao, Fangyu Mo, Tong Liu, Yue Chen
Summary: This study identified eight StGPXL genes in potatoes for the first time through genome-wide bioinformatics analysis and investigated their expression patterns. The results showed that StGPXL1 was upregulated under high-temperature stress, indicating its involvement in potato defense against high temperatures. StGPXL4 and StGPXL5, on the other hand, were downregulated. Under drought stress, StGPXL1, StGPXL2, StGPXL3, and StGPXL6 were upregulated, suggesting their role in potato defense against drought stress. After MeJA hormone treatment, StGPXL6 expression was significantly upregulated, indicating its involvement in the chemical defense mechanism of potatoes. The expression of all StGPXL genes was inhibited under biotic stress, indicating that GPXL is a multifunctional gene family that confers resistance to various stresses. This study deepens the understanding of the potato GPXL gene family's function and provides comprehensive information for further analysis and molecular breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bhaba Krishna Das, Amit Kumar, Sreeshma Nellootil Sreekumar, Kannapiran Ponraj, Kaustubh Gadave, Saravanan Kumar, V. Mohan Murali Achary, Pratima Ray, Malireddy K. Reddy, Arulandu Arockiasamy
Summary: Ascorbate is an important cellular antioxidant that can be easily oxidized to dehydroascorbate (DHA). Recycling of DHA is crucial for maintaining cellular homeostasis and preventing oxidative stress. Plant DHARs, in conjunction with GSH, play a vital role in this process, and their kinetics relative to human counterparts need further investigation. This study reports the highest turnover rate for a plant DHAR from stress adapted Pennisetum glaucum (PgDHAR) and shows that HsCLICs have a significantly lower reduction rate of DHA. Furthermore, the catalytic cysteine from both homologs is susceptible to oxidation to varying degrees.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Sahil Mehta, A. Kumar, V. Mohan Murali Achary, Prakash Ganesan, Asharani Patel, Asmita Singh, Neelmani Rathi, T. K. Das, Shambhu Krishan Lal, Malireddy K. Reddy
Summary: This study found that potassium phosphite (Phi) has antifungal and defense elicitor activity against rice blast disease, suggesting its potential as an alternative to toxic fungicides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Varakumar Panditi, Dhirendra Fartyal, Vijay Sheri, Aakrati Agarwal, Sahil Mehta, Shambhu Krishan Lal, Ajay Kumar Venkatapuram, Hemangini Parmar, Sangeetha Karippadakam, Afreen Rashid, Malireddy K. Reddy, V. Mohan Murali Achary
Summary: This research confirms the moderate tolerance of the bacterial gene igrA against glyphosate in transgenic rice plants. The study highlights the modest detoxification efficiency of the igrA gene, regardless of its cellular localization. The potential application of directed evolution strategies may lead to the development of crops with higher glyphosate tolerance.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Plant Sciences
Banavath Jayanna Naik, Ganesh Shimoga, Seong-Cheol Kim, Mekapogu Manjulatha, Chinreddy Subramanyam Reddy, Ramasubba Reddy Palem, Manu Kumar, Sang-Youn Kim, Soo-Hong Lee
Summary: The CRISPR/Cas9 method, combined with nanotechnology, has great potential in crop development, offering solutions to global food scarcity and improving crop yield and quality.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Agronomy
Anirudha Chattopadhyay, Jyotika Purohit, Sahil Mehta, Hemangini Parmar, Sangeetha Karippadakam, Afreen Rashid, Alexander Balamurugan, Shilpi Bansal, Ganesan Prakash, V. Mohan Murali Achary, Malireddy K. Reddy
Summary: In the face of the current food crisis, improving rice productivity per hectare is a top priority for scientists and breeders. Traditional breeding techniques have played a significant role in developing desired rice varieties, but are insufficient to meet the projected food security needs of the future. Precise crop genome editing using tools such as TALENs and CRISPR/Cas9 holds the potential to address this multifaceted crisis. This article summarizes the advancements in rice genome editing tools over the past decade, particularly in enhancing biotic stress tolerance.
Article
Plant Sciences
Md Sifatul Islam, Munira Mohtasim, Tahmina Islam, Ajit Ghosh
Summary: This study identified 19 ALDH genes in Sorghum bicolor and found that they clustered with monocot rice and maize in the phylogenetic tree. Expression analysis revealed that several SbALDH genes were expressed in various tissues, developmental stages, and in response to abiotic stresses, with the majority of the genes showing high responsiveness to drought stress. The presence of cis-acting elements in the promoter region of these genes might play a significant role in drought tolerance.
Article
Plant Sciences
Ajit Ghosh, Md Sifatul Islam, Nazmir Binta Alam, Ananda Mustafiz, Tahmina Islam
Summary: This study provides a comprehensive analysis of glutathione (GSH) metabolizing genes in Arabidopsis and rice, revealing their involvement in plant response to stress and developmental changes. The presence of stress-specific cis-regulatory elements in the promoter region of these genes suggests their direct regulation under stress conditions. Additionally, exogenous GSH has been shown to regulate the expression of GSH metabolizing genes. This research highlights the potential of GSH metabolizing genes as a genetic resource for improving plant stress tolerance.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Multidisciplinary Sciences
Tahmina Islam, M. K. Reddy
Summary: Non-essential toxic heavy metals like cadmium (Cd2+) have adverse effects on the growth and development of plants and can potentially harm human health. This study focused on the role of a peroxidase gene family member, PgGPx, in rice under Cd2+ stress. The overexpression of PgGPx enhanced Cd2+ tolerance in transgenic rice plants and improved oxidative stress response by increasing the activity of antioxidant enzymes. These findings provide insights for developing strategies to engineer Cd2+ stress tolerance in economically important crop plants.
Article
Plant Sciences
Badri Nath Singh, V. Mohan Murali Achary, Ajay Kumar Venkatapuram, Hemangini Parmar, Sangeetha Karippadakam, Sudhir Kumar Sopory, Malireddy K. Reddy
Summary: This study investigated plant DNA topoisomerase II (Topo II) and found that truncated polypeptides of Nicotiana tabacum topo II (NtTopoII) failed to target the yeast nucleus and did not rescue the temperature-sensitive phenotype. However, full-length NtTopoII was able to restore the phenotype and localized to the yeast nucleus, suggesting the presence of a potent nuclear localization signal (NLS). Biochemical characterization revealed the ATP binding and hydrolysis region of NtTopoII located at the N-terminal region and that all truncated NtTopoII polypeptides were detrimental for in vitro supercoiled DNA relaxation and/or DNA nicking and ligation activity. The disruption of coordinated macromolecular interface movements and dimer interactions in truncated NtTopoII may be responsible for the loss of topoisomerase activity.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Saima Akhter, Asif Ahmed Sami, Tamanna Islam Toma, Bushrat Jahan, Tahmina Islam
Summary: This study carries out in-depth in-silico characterization of the CCoAOMT gene family in two jute species, identifying 6 CCoAOMT gene members in each species and revealing high syntenic conservation between them. The study also shows how the identified CCoAOMT genes form four clusters in the phylogenetic tree, and sheds light on lignin deposition patterns in jute stems and their response to abiotic stresses. Expression profiling using qPCR highlights considerable alterations of CCoAOMT transcripts under various abiotic stresses and hormonal treatment.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Bhaba Krishna Das, Wajahat Ali Khan, Sreeshma Nellootil Sreekumar, Kannapiran Ponraj, V. Mohan Murali Achary, Elluri Seetharami Reddy, D. Balasubramaniam, Anmol Chandele, Malireddy K. Reddy, Arulandu Arockiasamy
Summary: Plant dehydroascorbate reductases (DHARs) are soluble antioxidant enzymes that protect plants from oxidative stress. Our study reveals the existence of a novel membrane-integrated form of DHAR in plants, which can also conduct ions and is translocated to the plasma membrane under induced oxidative stress.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Kirti Shila Sonkar, V. Mohan Murali Achary, Sibasis Sahoo, Malireddy K. Reddy, Arulandu Arockiasamy
Summary: In this study, we isolated a gene encoding the MDHAR enzyme from heat stress-adapted pearl millet and characterized it using enzyme kinetics, thermal stability assays, and crystal structure determination. Our results indicate that PgMDHAR is a more robust enzyme than its rice counterpart (OsMDHAR). We solved the crystal structure of PgMDHAR and found that the enzyme has a more compact structure and greater stability than OsMDHAR.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Goutam Kumar Tanti, Prachi Pandey, Smriti Shreya, Buddhi Prakash Jain
Summary: The Striatin family of proteins, including Striatin, SG2NA, and Zinedin, functions as signaling scaffolds with multiple protein-protein interaction domains. Zinedin and SG2NA are involved in cancer cell proliferation. SG2NA undergoes alternative splicing and gives rise to different isoforms regulated by tissues. In addition to alternative splicing, SG2NA and Striatin both play significant roles in estrogen signaling, neuroprotection, cancer, and cell cycle regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Plant Sciences
Shambhu Krishan Lal, Sahil Mehta, Dhandapani Raju, V. Mohan Murali Achary, Ajay Kumar Venkatapuram, Shashank Kumar Yadav, Hemangini Parmar, Rakesh Pandey, Varakumar Panditi, Vijay Sheri, Anil Kumar Singh, Viswanathan Chinnusamy, Malireddy K. K. Reddy
Summary: Nitrogen is an essential macronutrient for crop productivity, but its low use efficiency leads to environmental degradation and higher input costs for farmers. This study developed a genetic strategy to improve nitrogen use efficiency in rice plants by enhancing the activity of glutamine synthetase (GS), resulting in enhanced growth and productivity. The increased nitrogen assimilation positively affected photosynthetic efficiency and C/N balance in the transgenic rice plants. However, insufficient C supply may reduce the positive effect of GS overexpression, suggesting the need for refined strategies to improve both N and C assimilation simultaneously.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Shilpi Bansal, Mallana Gowdra Mallikarjuna, Bhaskar Reddy, Alexander Balamurugan, V. Mohan Murali Achary, Malireddy K. Reddy, Aundy Kumar, Ganesan Prakash
Summary: Blast disease caused by Magnaporthe species is a major threat to agricultural cereal crops, and understanding the molecular basis of pathogen virulence factors is crucial for developing long-term resistance. This study characterized the virulence factors from two major species of Magnaporthe, M. oryzae and M. grisea, and identified their potential target genes and functional roles. PCR analysis confirmed the presence of these virulence factors in Magnaporthe isolates infecting different host crops. This research provides valuable insights for studying Magnaporthe-host interactions and developing resistant cultivars through genetic modification.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)