Review
Biochemical Research Methods
Mattia Furlan, Stefano de Pretis, Mattia Pelizzola
Summary: Despite RNA abundance being commonly assumed as a proxy for transcriptional activity, it is now possible to calculate how synthesis, processing, and degradation rates collectively determine the abundance of each gene's RNA. Different transcriptional outputs can correspond to different combinations of kinetic rates, indicating the existence of markedly different modes of gene expression regulation and their profound effects on gene self-expression regulation.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Pharmacology & Pharmacy
Andrea Cignarella, Carlotta Boscaro, Mattia Albiero, Chiara Bolego, Matthias Barton
Summary: Post-translational and epigenetic regulation are important mechanisms controlling gene and protein function. Estrogens can modulate protein turnover through various pathways, including post-transcriptional and post-translational mechanisms. MicroRNAs play crucial roles in cancer and are regulated by estrogens.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2023)
Review
Biochemistry & Molecular Biology
De-Li Shi
Summary: Myocardial injury leads to cell death and heart failure. While the adult mammalian heart has limited regenerative capacity, early postnatal mammals and adult lower vertebrates can fully regenerate their hearts. RNA-binding proteins play a crucial role in regulating the survival and proliferation of cardiomyocytes following cardiac injury. By reprogramming various cellular processes, such as protein synthesis, metabolism, growth, and plasticity, RNA-binding proteins regulate heart regenerative capacity. Manipulating the activity of RNA-binding proteins may offer potential therapeutic interventions for cardiovascular disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Di Liu, Ruiru Li, Siqi Xu, Maohua Shi, Yu Kuang, Jingnan Wang, Chuyu Shen, Qian Qiu, Liuqin Liang, Youjun Xiao, Hanshi Xu
Summary: The increased expression of SMOC2 in FLSs may contribute to synovial aggression and joint destruction in RA, and SMOC2 may serve as a potential target against RA. Knockdown of SMOC2 can reduce the migration and invasion of RA FLSs by regulating cytoskeleton remodeling and controlling MYO1C expression. Additionally, intra-articular treatment with Ad-shRNA-SMOC2 can attenuate synovial inflammation as well as bone and cartilage erosion in rats with collagen-induced arthritis.
CELL DEATH & DISEASE
(2022)
Review
Cell Biology
Qiuqin Ma, Shihui Long, Zhending Gan, Gianluca Tettamanti, Kang Li, Ling Tian
Summary: Autophagy, a widely conserved process in eukaryotes, is regulated by nutrient deprivation, energy stress, and other unfavorable conditions through multiple pathways. It is primarily governed at the RNA and protein levels in a synergistic manner.
Article
Cell Biology
Chao Yang, Chencheng Yao, Zhiyong Ji, Liangyu Zhao, Huixing Chen, Peng Li, Ruhui Tian, Erlei Zhi, Yuhua Huang, Xia Han, Yan Hong, Zhi Zhou, Zheng Li
Summary: ELAVL2 is a conserved and SSCs-enriched RBP that is down-regulated in NOA, regulating spermatogonia proliferation and apoptosis by promoting protein expression of targets.
CELL PROLIFERATION
(2021)
Review
Immunology
Sohei Makita, Hiroaki Takatori, Hiroshi Nakajima
Summary: Post-transcriptional regulation of inflammatory genes is mediated by ZFP36 family proteins, which bind to mRNA and lead to target mRNA decay. Despite structural similarities, ZFP36 family members have distinct functions and regulate different target mRNAs, likely due to cell-type-specific expression patterns.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Wenhu Chen, Liqiang Hu, Xuemei Lu, Xiaofei Wang, Changan Zhao, Chen Guo, Xiaoyan Li, Yuqin Ding, Hongguang Zhao, Dongdong Tong, Lifang Wang, Chen Huang
Summary: This study reveals that MEX3A is highly expressed in breast cancer tissues and is associated with poor prognosis. Further experiments demonstrate that knockdown of MEX3A inhibits the proliferation and migration of breast cancer cells, as well as the growth of xenograft tumors. Additionally, MEX3A activates the PI3K/AKT signaling pathway by binding to IGFBP4 mRNA, which promotes cell cycle progression and cell migration.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Microbiology
Laura Piel, K. Shanmugha Rajan, Giovanni Bussotti, Hugo Varet, Rachel Legendre, Caroline Proux, Thibaut Douche, Quentin Giai-Gianetto, Thibault Chaze, Thomas Cokelaer, Barbora Vojtkova, Nadav Gordon-Bar, Tirza Doniger, Smadar Cohen-Chalamish, Praveenkumar Rengaraj, Celine Besse, Anne Boland, Jovana Sadlova, Jean-Francois Deleuze, Mariette Matondo, Ron Unger, Petr Volf, Shulamit Michaeli, Pascale Pescher, Gerald Spath
Summary: Genome instability plays a crucial role in the adaptation and regulation of the protozoan parasite Leishmania, utilizing post-transcriptional and epitranscriptomic regulation to adapt to its host environment.
Review
Cell Biology
Di Wang, Rong Ye, Zhaokui Cai, Yuanchao Xue
Summary: Pervasive transcription of the human genome generates a large amount of noncoding RNAs that play crucial roles in various biological processes. These RNAs interact with different RNA substrates through RNA-RNA interactions (RRIs), which are facilitated by RNA-binding proteins. High-throughput methods have been developed to study RRIs and these efforts have expanded our understanding of gene regulation.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Review
Biochemistry & Molecular Biology
Marcos Morgan, Lokesh Kumar, Yin Li, Marine Baptissart
Summary: Multiple RNA pathways are essential in producing functional sperm, with a particular focus on 3' end modifications. Studies have shown a correlation between RNA 3' end metabolism and male germ cell differentiation, as well as the importance of RNA pathways in maintaining genomic integrity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Weijin Xu, Jeetayu Biswas, Robert H. Singer, Michael Rosbash
Summary: RNA binding proteins (RBPs) regulate post-transcriptional processes in cells, and it is crucial to identify their in vivo targets for understanding their function. Standard techniques for profiling RBP targets have limitations in certain situations, but new genetic approaches have been developed for such circumstances. These approaches, including TRIBE, RNA tagging, and STAMP, provide useful tools for studying post-transcriptional regulation and RBP identification, with potential therapeutic implications.
Article
Genetics & Heredity
Kendra Reynaud, Molly Brothers, Michael Ly, Nicholas T. Ingolia
Summary: The yeast RNA-binding protein Mrn1 is involved in controlling a gene expression program that links mitochondria with the yeast cell wall, impacting cell wall organization and biogenesis. Mrn1 destabilizes target mRNAs during glucose fermentation, but this repression is relieved when cells switch to respiratory growth, leading to expanded mitochondria and quicker adaptation to respiration in cells lacking MRN1. The SUN domain protein Nca3, a key target of Mrn1, plays a crucial role in sculpting mitochondria and the cell wall in response to changing growth conditions.
Review
Biochemistry & Molecular Biology
Noof Aloufi, Aeshah Alluli, David H. Eidelman, Carolyn J. Baglole
Summary: Chronic obstructive pulmonary disease (COPD) is a respiratory disorder characterized by chronic inflammation and emphysema, primarily caused by cigarette smoke. Various cellular processes are altered by cigarette smoke, including post-transcriptional regulation of mRNAs. RNA-binding proteins, microRNAs, and long non-coding RNAs play key roles in regulating RNA biology and may contribute to the pathogenesis of COPD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
De-Li Shi
Summary: RBM24 is an RNA-binding protein that plays critical roles in embryonic development and tissue homeostasis. It is frequently dysregulated in human cancers and can act as both a tumor suppressor and oncogene, depending on the context. Its activity is regulated by protein-protein interactions and post-translational modifications. However, the molecular mechanisms underlying its function in tumor growth and metastasis are still unclear.
Article
Biochemistry & Molecular Biology
Nan Wu, Dong Yang, Zhenchao Wu, Mengdie Yan, Pengju Zhang, Yi Liu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Qing Liu, Wei Sheng, Yuan Ma, Junhui Zhen, Satyajit Roy, Chowdhury Alvira Jafar, Wei Xin, Qiang Wan
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Tingting Lv, Yanyan Hu, Yuan Ma, Junhui Zhen, Wei Xin, Qiang Wan
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Cell Biology
Nan Wu, Zhenchao Wu, Jian Sun, Mengdie Yan, Bingbing Wang, Xintong Du, Yi Liu
Article
Biochemistry & Molecular Biology
Yuan Ma, Ruijuan Yan, Qiang Wan, Bo Lv, Ying Yang, Tingting Lv, Wei Xin
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Xia Gu, Yi Liu, Na Wang, Junhui Zhen, Bo Zhang, Shaoshuai Hou, Zhengguo Cui, Qiang Wan, Hong Feng
Summary: In diabetic kidney disease, decreased expression of mitochondrial ribosomal protein L7/L12 (MRPL12) leads to mitochondrial dysfunction, and Nrf2 may act as a potential transcription factor for MRPL12. Therefore, Nrf2 may play a crucial role in regulating mitochondrial function in DKD.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Tingting Lv, Yao Lu, Yi Liu, Hong Feng, Chensheng Li, Wei Sheng, Zhengguo Cui, Suwei Zhu, Xia Gu, Zhe Yang, Qiang Wan
Summary: The study suggests that GCN5L1 plays a critical role in maintaining cellular redox homeostasis in DKD. Deletion of GCN5L1 can effectively ameliorate oxidative stress-induced renal injury in DKD.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Cell Biology
Ying Yang, Chensheng Li, Xia Gu, Junhui Zhen, Suwei Zhu, Tingting Lv, Qiang Wan, Yi Liu
Summary: ING2 plays a crucial role in regulating mitochondrial respiration in tubular epithelial cells, potentially maintaining mitochondrial homeostasis by modulating mtDNA transcription and respiratory chain components. Additionally, ING2 regulates the ubiquitination of a mitochondrial transcription factor MRPL12, and has a protective effect on tubular epithelial cells against acute ischemic injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Yitong Han, Yi Liu, Junhui Zhen, Shaoshuai Hou, Bo Zhang, ZhengGuo Cui, Qiang Wan, Hong Feng
Summary: The well-documented tumor suppressor p53 regulates the mitochondrial biogenesis through the transcriptional regulation of MRPL12. The p53/MRPL12 pathway plays a critical role in maintaining mitochondrial content and coordinating mitochondrial biogenesis and oxidative phosphorylation.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Tingting Lv, Yu Zhang, XingZhao Ji, Shengnan Sun, Li Xu, Weixia Ma, Yi Liu, Qiang Wan
Summary: GCN5L1 regulates the acetylation and intracellular trafficking of TFAM, playing an important role in AKI-associated mitochondrial impairment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Xingzhao Ji, Lingju Chu, Dun Su, Jian Sun, Peng Song, Shengnan Sun, Ying Wang, Qia Mu, Yi Liu, Qiang Wan
Summary: Acute kidney injury (AKI) is a serious disease with no effective treatment. Abnormal opening of mitochondrial permeability transition pore (MPTP) is an important pathological process in AKI. MRPL12, a mitochondrial ribosomal protein, plays a crucial role in stabilizing MPTP and maintaining mitochondrial membrane homeostasis in renal tubular epithelial cells (TECs). Reduced expression of MRPL12 in TECs during AKI leads to MPTP abnormal opening and cell apoptosis. MRPL12 overexpression protects TECs from MPTP abnormal opening and apoptosis during hypoxia/reoxygenation (H/R). MRPL12-ANT3 axis could be a potential intervention target for AKI treatment.
Article
Biochemistry & Molecular Biology
Xingzhao Ji, Xiaoli Yang, Xia Gu, Lingju Chu, Shengnan Sun, Jian Sun, Peng Song, Qian Mu, Ying Wang, Xiaoming Sun, Dun Su, Tong Su, Shaoshuai Hou, Yao Lu, Chen Ma, Mingqiang Liu, Tianyi Zhang, Weiying Zhang, Yi Liu, Qiang Wan
Summary: Diabetic kidney disease (DKD) is the leading cause of chronic kidney disease and mortality in diabetic patients. This study reveals that mitochondrial ribosomal protein L12 (MRPL12) plays a crucial role in DKD by affecting mitochondrial function. Cullin3 (CUL3), an E3 ubiquitin ligase, has been identified to mediate the ubiquitination of MRPL12, leading to mitochondrial biosynthesis dysfunction. The findings suggest a potential therapeutic strategy for DKD by targeting the CUL3-MRPL12 pathway.
Article
Biology
Yao Lu, Xia Gu, Qiang Wan, Hong Feng, Yi Liu
Summary: Immunofluorescence is a technique for visualizing specific molecules in cells using antibodies; optimizing antibody concentrations for different cell types is necessary; a variety of antibodies from different species can be used to analyze protein co-localization and expression.
Article
Biochemical Research Methods
Xia Gu, Yuan Ma, Yi Liu, Qiang Wan
Summary: Mitochondria play crucial roles in cellular energy metabolism, generating most of the intracellular ATP through respiration. The Cell Mito Stress Test is a common method for measuring key parameters of mitochondrial respiration, demonstrated here using the human cell line HK-2 as an example.
Article
Respiratory System
Jian Sun, Xianmin Gu, Nan Wu, Pengju Zhang, Yi Liu, Shujuan Jiang
RESPIRATORY RESEARCH
(2018)