Article
Environmental Sciences
Jing Tian, Yongjing Li, Huiru Fu, Liting Ren, Yameng He, Shangyu Zhai, Ben Yang, Qi Li, Nannan Liu, Shikai Liu
Summary: This study demonstrated the critical role of CYP17A1-like in Cd detoxification in Pacific oysters, providing a new perspective towards unraveling detoxification mechanisms of bivalves under heavy metal stress. The RNA interference experiments confirmed the importance of CYP17A1-like in Cd detoxification, leading to significant tissue damage in oysters and increased activities of antioxidant enzymes. Dual luciferase reporter assay revealed potential transcriptional regulation of CYP17A1-like by MTF-1 in oysters.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Fisheries
Jiafeng Fang, Chengxun Xu, Qi Li
Summary: This study investigated the effect of inbreeding on gene expression in the Pacific oyster. It was found that there were differentially expressed genes associated with inbreeding depression. This research contributes to a better understanding and management of inbreeding depression in bivalves, and may potentially benefit oyster breeding in aquaculture.
Article
Chemistry, Medicinal
Monika Dhanji-Rapkova, Andrew D. Turner, Craig Baker-Austin, Jim F. Huggett, Jennifer M. Ritchie
Summary: A potent and heat-stable toxin, TTX, accumulates in various marine bivalve species, including Pacific oysters, with the highest concentrations found in the digestive gland. TTX levels vary significantly between different geographical locations and within populations of oysters, suggesting high variability in toxin distribution.
Article
Environmental Sciences
Xuekai Zhang, Chao Fan, Jinlong Li, Xingzhi Zhang, Qiongzhen Li, Zhaoping Wang
Summary: This study analyzed the molecular processes in hybrids of Pacific oyster under hypoxia stress and identified significant differentially expressed genes related to cell survival and immune response.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Huiru Fu, Zexin Jiao, Yongjing Li, Jing Tian, Liting Ren, Fuqiang Zhang, Qi Li, Shikai Liu
Summary: This study identified 66 TRP genes in Pacific oyster with significant gene expansion and tandem duplication. The analysis of RNA-Seq data after acute heat stress revealed a set of heat-inducible TRPs. Further study under chronic heat stress and comparison between two oyster species led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Arno Bringer, Jerome Cachot, Emmanuel Dubillot, Gregoire Prunier, Valerie Huet, Christelle Clerandeau, Louise Evin, Helene Thomas
Summary: This study focused on the impacts of aged aquaculture microplastics (MPs) on Pacific oysters. The results showed that MPs had no significant effect on the growth of adult oysters, but significantly increased the mortality rate of bivalves exposed to MPs. In addition, ingestion of MPs in oyster tissues and the presence in biodeposits were observed. Furthermore, the study found that MPs had effects on the swimming behavior, development, and growth of the offspring of exposed oysters.
ENVIRONMENTAL POLLUTION
(2022)
Article
Chemistry, Applied
Han Li, Xiaoyu Xia, Zhenyu Wang, Jiachen Zang, Ming Du
Summary: The study investigated the structure and stability characteristics of oyster ferritin by adding Fe2+ to it. The results showed that the protein structure of ferritin remained largely unchanged after adding Fe2+ and the sample with 1000 Fe2+ exhibited good stability under storage and high temperature conditions.
Article
Fisheries
Ping Wei, Wenwen Yang, Wei Wang, Yinan Li, Xiaoxue Yan, Wei Wu, Sicong Wang, Jiejie Sun, Lingling Wang, Linsheng Song
Summary: In this study, a MASP-like protein, CgMASPL-2, was identified from the Pacific oyster. CgMASPL-2 was found to be expressed in multiple tissues, with the highest expression in haemolymph. It was shown that CgMASPL-2 can directly sense microbes and regulate the mRNA expressions of inflammatory factors.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Chenyang Yue, Qi Li, Hong Yu
Summary: This study focused on the miRNA and mRNA expression profiles in the gonads of Pacific oyster during different stages of gametogenesis, revealing sex-specific patterns and potential regulatory mechanisms in sexual development. The findings suggest that certain sex-biased miRNAs might participate in the regulation of gametogenesis, providing insights into the molecular mechanisms of bivalve sex differentiation.
Article
Environmental Sciences
Thomas Sol Dourdin, Guillaume Riviere, Alexandre Cormier, Carole Di Poi, Killian Guyomard, Manuella Rabiller, Farida Akcha, Thierno Bah Sadialiou, Pauline Le Monier, Rossana Sussarellu
Summary: Early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life history traits in the Pacific oyster, Crassostrea gigas. The study found that exposure to a combination of 18 pesticides and metals at environmental concentrations during the embryo-larval stage resulted in disturbed swimming behavior, increased size and weight at specific time points, and altered expression of development-related genes. Additionally, DNA methylation analyses showed significant modifications in exposed larvae, marked by a demethylation trend.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Huijuan Li, Qi Li, Hong Yu, Shaojun Du
Summary: Paramyosin, a key component of invertebrate muscles, was isolated from Pacific oyster in this study. Expression analysis revealed strong expression of CgPM during larval myogenesis, particularly in adductor muscles, providing a basis for further functional studies.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Inouye
Summary: The genomic structure of the Cypridina luciferase gene in Vargula hilgendorfii was found to contain multiple exons and introns, with conserved splicing sites but varying intron lengths. PCR amplification and DNA sequence analysis revealed the presence of multiple luciferase gene groups in the genome of a single V. hilgendorfii specimen.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Engineering, Multidisciplinary
Faming Yang, Zhouchi Wang, Di Zhao, Le Hu, Shenghao Cui, Liqi Chen, Tingting Guo, Panpan Pan, Jingdi Chen
Summary: In this study, multifunctional supramolecular materials (CNPs) were prepared from Crassostrea gigas meats and were found to promote dermal wound healing in in vitro and in vivo experiments. The CNPs consisted of peptides that self-assemble into nano-structures and possess procoagulant, antioxidant, and cell proliferation-promoting effects. The administration of CNPs through topical application and oral routes showed promising results in inhibiting inflammation, promoting fibroblast proliferation and revascularization, accelerating the epithelialization process, achieving balanced tissue repair, and promoting hair follicle regeneration. This study presents a simple method for preparing a safe and multifunctional self-assembling peptides material with great potential in soft tissue repair.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Food Science & Technology
Yuyang Ma, Yahui Wang, Suisui Jiang, Mingyong Zeng
Summary: This study investigated the effect of gelatin on the formation of oyster protein gel and the interaction mechanism between gelatin and oyster protein. The results showed that gelatin improved the texture properties of oyster protein gel and enhanced the gelation process. In addition, new hydrogen bonds and beta-sheets structures were formed between gelatin and oyster protein.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Roni Nugraha, Thimo Ruethers, Aya C. Taki, Elecia B. Johnston, Shaymaviswanathan Karnaneedi, Sandip D. Kamath, Andreas L. Lopata
Summary: This study investigates the IgE sensitisation profiles of patients with oyster allergy to raw and heated Pacific oyster extract. Tropomyosin is identified as the major allergen responsible for oyster allergy, and the study also reveals partial cross-reactivity and/or co-sensitisation between oyster, prawn, and dust mite allergens. This research emphasizes the importance of using recombinant tropomyosin in improved diagnostics and immunotherapy.
