Article
Forestry
Shaofei Dang, Lifeng Zhang, Suying Han, Liwang Qi
Summary: A simple and efficient protocol for the genetic transformation of Japanese larch was developed and multiple transgenic cell lines were obtained. However, the expression levels of exogenous genes in the transgenic system were relatively low, possibly due to the large genome of Japanese larch.
Article
Plant Sciences
Xudong Ye, Zarir Vaghchhipawala, Edward J. Williams, Changlin Fu, Jinyuan Liu, Fengming Lu, Erin L. Hall, Shirley X. Guo, LaRee Frank, Larry A. Gilbertson
Summary: Efficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre recombinase expression. Different crops require diverse promoters to drive Cre expression, and the efficiency of marker-free progeny generation varies among different crops.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Xudong Ye, Ashok Shrawat, Lorena Moeller, Rebecca Rode, Anatoly Rivlin, David Kelm, Brian J. Martinell, Edward J. Williams, Anthony Paisley, David R. Duncan, Charles L. Armstrong
Summary: Transgenic wheat plants with fertility have been successfully regenerated through direct transformation of mechanically isolated mature embryos from field-grown seed. Centrifugation of the embryos in the presence of Agrobacterium is important for efficient T-DNA delivery. The transformed embryos formed multiple buds/shoots on high-cytokinin medium and directly regenerated into transgenic shoots on hormone-free medium. Rooted transgenic plantlets were obtained within 10-12 weeks. Optimization of the protocol reduced the occurrence of chimeric plants to below 5%.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hua Qi, Xue Lei, Yao Wang, Shan Yu, Ting Liu, Shun-Kang Zhou, Jin-Yu Chen, Qin-Fang Chen, Rong-Liang Qiu, Liwen Jiang, Shi Xiao
Summary: 14-3-3 proteins, specifically 14-3-3k and 14-3-3j, modulate autophagy dynamics by promoting the proteolysis of ATG13 through SINAT-mediated degradation. These proteins directly interact with SINATs and ATG13 in both vitro and vivo. Their association with phosphorylated ATG13 is crucial for ATG13 stability and formation of the ATG1-ATG13 complex.
Article
Biochemistry & Molecular Biology
Shabnam K. Saifi, Nishat Passricha, Renu Tuteja, Manoj Nath, Ritu Gill, Sarvajeet Singh Gill, Narendra Tuteja
Summary: This study provides the first direct evidence of the novel function of RuvBL in boosting abiotic stress tolerance in plants. By genetically engineering the OsRuvBL gene into rice genome, the transgenic lines showed better performance under abiotic stresses. This approach has successfully developed abiotic stress resilient smart crops.
Article
Biochemistry & Molecular Biology
Qiyu Luo, Shu Chen, Hai Nian, Qibing Ma, Yuyao Ding, Qinwen Hao, Jiping Wei, Jinesh D. Patel, Joseph Scott McElroy, Yaoguang Liu, Yong Chen
Summary: In this study, a successful genetic transformation method for goosegrass was established. Conditions for seed germination and callus induction were optimized. The overexpression of the paraquat-resistant EiKCS gene in paraquat-susceptible goosegrass showed stable inheritance of paraquat resistance. This study is valuable for future research on herbicide resistance using similar methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Elena A. A. Vasyutkina, Yulia A. A. Yugay, Valeria P. P. Grigorchuk, Olga V. V. Grishchenko, Maria R. R. Sorokina, Yulia L. L. Yaroshenko, Olesya D. D. Kudinova, Varvara D. D. Stepochkina, Victor P. P. Bulgakov, Yury N. N. Shkryl
Summary: This study evaluated the effects of abiotic stresses and chemical elicitors on CQAs biosynthesis and cT-DNA gene expression in sweet potato cell culture. Results showed that cT-DNA genes were not expressed in cell culture, and the studied conditions weakly affected their transcriptional levels. However, overaccumulation of the Ib-rolB/C gene increased the content of CQAs in sweet potatoes. The study provides new insights into the molecular mechanisms of CQAs biosynthesis in cell culture of naturally transgenic food crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Lei Yan, Liu Zeng, Ali Raza, Yan Lv, Xiaoyu Ding, Yong Cheng, Xiling Zou
Summary: This study aimed to investigate the beneficial role of exogenous inositol in improving cold stress tolerance in rapeseed. The results revealed that inositol positively regulated cold tolerance by increasing calcium ion influx in rapeseed. The inositol-Ca2+ pathway was found to play a key role in cold tolerance. This study provides new insights into the mechanism of rapeseed cold tolerance and introduces a feasible method for improving rapeseed cold tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Aparajita Choudhury, Manchikatla Rajam
Summary: Genetic transformation in legume plants is an important area of agricultural research. Despite some progress in improving crop yields, challenges such as recalcitrance and genotype-dependence still exist. Future research may focus on overcoming these obstacles and further increasing yields.
PLANT CELL REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Saleem Ur Rahman, Muhammad Omar Khan, Rahim Ullah, Fayaz Ahmad, Ghulam Raza
Summary: Plant transformation through Agrobacterium-mediated transformation is a technique that mimics the natural agrobacterium system to introduce gene(s) into crops. It has shown successful genetic transformation in various crop species for different traits. Although it has advantages like stable transgene integration and cost-effectiveness, limitations such as crop recalcitrance, low transformation efficiency, transgene integration, and off-target effects exist. Therefore, it is crucial to explore the limitations and possible solutions to enhance the efficiency of Agrobacterium-mediated transformation.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Biotechnology & Applied Microbiology
Xiaoyu Zhang, Gencheng Xu, Chaohua Cheng, Lei Lei, Jian Sun, Ying Xu, Canhui Deng, Zhigang Dai, Zemao Yang, Xiaojun Chen, Chan Liu, Qing Tang, Jianguang Su
Summary: This study successfully generated gene-edited plants in Cannabis sativa using an Agrobacterium-mediated transformation method, improving shoot regeneration efficiency and editing the phytoene desaturase gene with CRISPR/Cas9 technology.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li
Summary: In this study, the effects of different factors on the genetic transformation efficiency of broccoli were explored. Hypocotyl explants were found to be more suitable for transgene and gene editing. The usage of hygromycin B at a concentration of 5 mg/L was more advantageous for selecting resistant broccoli sprouts, and genotype 19B42 exhibited the highest transformation rate. Additionally, subcellular localization confirmed the expression of glucoraphanin metabolism-related gene Bol031350 and clubroot resistance gene CRa in the cytoplasm and nucleus. These findings contribute to the improvement of genetic transformation and molecular breeding in Brassica oleracea crops.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel, Jose M. Segui-Simarro
Summary: Brassica napus is a species of high agronomic interest commonly used for studying various processes, including microspore embryogenesis. DH4079 and DH12075 lines, with high and low embryogenic response respectively, are ideal for investigating the mechanisms controlling embryogenesis induction. This study examined the genetic transformation ability of DH4079 plants and compared it with the already studied DH12075 plants. Results showed that DH12075 plants responded to genetic transformation, while DH4079 plants remained recalcitrant, failing to produce any regenerant. The study also tested transient transformation with Agrobacterium rhizogenes, where only DH12075 showed a response. These findings indicate that DH4079 is recalcitrant to Agrobacterium-mediated transformation.
Article
Biochemistry & Molecular Biology
Zhelong Lin, Lei Chen, Shanjie Tang, Mengjie Zhao, Tong Li, Jia You, Changqing You, Boshu Li, Qinghua Zhao, Dongmei Zhang, Jianli Wang, Zhongbao Shen, Xianwei Song, Shuaibin Zhang, Xiaofeng Cao
Summary: The lack of genome editing platforms has hindered the study and improvement of forage crops that can be grown on lands not suitable for other crops. This study established an efficient CRISPR/Cas9 genome editing method in a stress-tolerant forage grass called sheepgrass, and knocking out the TB1 gene significantly increased tiller number and biomass.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)