期刊
MOLECULAR BIOLOGY REPORTS
卷 40, 期 1, 页码 159-169出版社
SPRINGER
DOI: 10.1007/s11033-012-2045-6
关键词
Citrus reticulata Blanco; Self-incompatibility; SSH; Pistil; Expression analysis
资金
- National Natural Science Foundation of China [31000899]
- Research Fund for the Doctoral Program of Higher Education of China [20104404120015, 20114404110018]
- Guangdong Province Science Foundation of China [06025843]
- Science and Technology Planning Project of Guangzhou [2010r1-C771]
- 211 Construction Fund for Key Subjects of College of Horticulture, South China Agricultural University
Self-incompatibility (SI) is one important factor that can result in Citrus seedlessness. However, the molecular mechanism of SI in Citrus is not clear yet. To isolate the pistil's SI-related genes, a suppression subtractive hybridization library was constructed using mature pistils of 'Wuzishatangju' mandarin (SI) as the tester and mature pistils of 'Shatangju' mandarin (self-compatibility, SC) as the driver. 229 differentially expressed cDNA clones from 967 positive clones were sequenced and identified. Differentially expressed ESTs are possibly involved in the SI reaction of 'Wuzishatangju' through a regulating signaling pathway, serine/threonine phosphatase activity, receptor kinase, embryonic development, gibberellin stimulus, or transcription. 11 out of 36 SI candidate genes displayed different expression patterns in various tissues and stages after self- and cross-pollination of 'Wuzishatangju'. The expression of CaBP (WY65), a senescence-protease (WY372), an unknown gene (WY283), and a WRKY (WY17) were up-regulated in the styles of 'Wuzishatangju' while higher expression of WY190 was observed in styles of 'Shatangju'. Highest expression levels of WY65, WY372, an annexin (WY598), the zinc-finger protein (WY376), a C2-protein (WY291), and an unknown gene (WY318) were detected in styles at 3 days after self-pollination of 'Wuzishatangju' while lowest levels were observed in styles at 3 days after cross-pollination of 'Wuzishatangju' x 'Shatangju'. The potential involvement of these genes in the SI reaction is discussed.
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