4.5 Article

Time-course analyses of abscisic acid level and the expression of genes involved in abscisic acid biosynthesis in the leaves of Betula platyphylla

期刊

MOLECULAR BIOLOGY REPORTS
卷 39, 期 3, 页码 2505-2513

出版社

SPRINGER
DOI: 10.1007/s11033-011-1002-0

关键词

Betula platyphylla Suk; Abscisic acid; Gene expression; Real time RT-PCR; LC-MS/MS

资金

  1. Key Project of Chinese National Programs for Fundamental Research and Development (973 Program) [2009CB119100]
  2. Fundamental Research Funds for the Central Universities [DL09DA01]
  3. Chinese Natural Science Foundation [30972385]
  4. Heilongjiang Postdoctoral Financial Assistance [LBH-Z09287]

向作者/读者索取更多资源

Abscisic acid (ABA) is a plant hormone regulating the essential physiological processes of plants and plant responses to various environmental stresses. Recent studies revealed that the key enzymes involved in ABA biosynthesis were zeaxanthin epoxidase (ZEP), 9-cis-epoxycarotenoid dioxygenases (NCED), short-chain dehydrogenase/ reductase (SDR), and ABA-aldehyde oxidase (AAO). In this study, we cloned 12 unique genes potentially involved in ABA synthesis, including 5 of BpNCEDs, 1 of BpAAO, and 6 of BpZEPs from birch leaves. We analyzed the time-course expression of these 12 genes during the phase from May to August by real time reverse transcriptase-polymerase chain reaction (RT-PCR). Further, we determined birch endogenous ABA content during this period by liquid chromatography tandem mass spectrometry (LC-MS/MS). The results indicated that ABA level significantly (P < 0.05) increased during the early stages of development and ABA was present in birch leaves throughout the studied period, suggesting that endogenous ABA is necessary for the normal growth and development of plants. The correlation between the expression of above-mentioned genes and ABA levels was analyzed by Pearson correlation analysis. The results revealed that the expression of three genes, namely, BpNCED4, BpNCED5 and BpZE6 significantly (P < 0.05) correlated with the ABA level. Therefore, these three genes may play important roles in ABA biosynthesis.

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