Article
Biochemistry & Molecular Biology
Jung Min Kim, Jae Il Lyu, Dong-Gun Kim, Nguyen Ngoc Hung, Jaihyunk Ryu, Jin-Baek Kim, Joon-Woo Ahn, Bo-Keun Ha, Soon-Jae Kwon
Summary: This study developed 58 novel EST-SSR markers from P. frutescens var. crispa and its mutant cultivar Antisperill, which can be used for population genetics and recombinant inbred line mapping analyses. These markers revealed significant genetic diversity and relationships among 94 P. frutescens genotypes, forming two major groups: Group I (var. crispa) and Group II (var. frutescens). The results provide insights into the genetic diversity and relationships of P. frutescens.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Multidisciplinary Sciences
Song Lu
Summary: In this study, a transcriptome analysis of Anoectochilus emeiensis was conducted, resulting in the identification of numerous unigenes and EST-SSR markers. The findings of this study will be valuable for future population genetic studies of A. emeiensis.
Article
Biochemistry & Molecular Biology
Jana Cmejlova, Martina Rejlova, Frantisek Paprstein, Radek Cmejla
Summary: The newly developed Ap17 in. SSR Genotyping Kit combines 17 SSR markers and covers all apple linkage groups in a one-tube reaction format. It has been successfully tested on 880 unique diploid apple germplasm accessions, and can easily discriminate triploid and tetraploid samples. The total probability of identity for the kit and the sample collection used was calculated to be 1.73 x 10(-22).
Article
Forestry
Ju Wang, Shuai Guo, Yongxin Zhang, Feng Zhang, Yufei Yun, Guosheng Zhang
Summary: This study sequenced the transcriptomes of open-pollinated progeny seedlings of Chinese pine from different provenances and analyzed the expression of genes involved in signaling pathways. It also detected EST-SSR loci and developed polymorphic primers for genetic diversity study and marker-assisted breeding.
Article
Plant Sciences
Bin Li, Noor Muhammad, Shufeng Zhang, Yunxin Lan, Yihan Yang, Shoukun Han, Mengjun Liu, Meng Yang
Summary: Jujube is a commercially important tree native to China due to its high nutritional value, widespread distribution, and diverse germplasm resources. The cultivation of jujube holds potential in providing food security and income for people living in arid and semi-arid regions. Simple Sequence Repeat (SSR) markers are essential for evaluating the genetic diversity and germplasm resources of jujube. However, traditional methods of SSR development are time-consuming and inefficient. A novel approach called Multiple-Genome-Based SSR identification (MGB-SSR) was developed to quickly screen for polymorphic SSRs in the jujube genome. This study successfully differentiated jujube germplasm using 12 SSR markers and provided valuable information for jujube researchers and breeders.
Article
Plant Sciences
Vikas Dwivedi, Lalita Pal, Shilpi Singh, Nagendra Pratap Singh, Swarup Kumar Parida, Debasis Chattopadhyay
Summary: This study identified a gene marker (CaWIP2) for regulating lateral root count in chickpea, which can be utilized to develop drought-tolerant, high-yielding cultivars.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Yi Lu, Jianling Chen, Bing Chen, Qianqian Liu, Hanlin Zhang, Liyuan Yang, Zhi Chao, Enwei Tian
Summary: This study provides a comprehensive investigation into the genetic diversity and population structure of F.hirta in southern China. The results reveal high genetic diversity and low population differentiation in this medicinal plant. The causes of the current genetic patterns are clarified, and insights are provided for the exploitation and conservation strategies of F.hirta.
Article
Plant Sciences
Liyi Xu, Pingping Li, Jingjing Su, Ding Wang, Yunbo Kuang, Zuyun Ye, Meixia Chen
Summary: In this study, 360 EST-SSR markers were developed using a transcriptome library. The analysis of genotyping data showed that 20 markers had high fingerprinting power and genetic diversity. These markers were recommended for analyzing the genetic diversity and parentage of 102 F1 hybrid individuals.
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
(2023)
Article
Genetics & Heredity
Bin Yin, Haiyang Wang, Shaoping Weng, Sedong Li, Jianguo He, Chaozheng Li
Summary: This study explores the potential of a (CT)n-SSR marker in disease resistance breeding and the hereditary property of disease resistance traits in shrimp offspring. The study finds that shrimp with shorter (CT)n repeats exhibit higher resistance to viral pathogens, while those with longer (CT)n repeats show higher tolerance to bacterial infection. These findings suggest that the (CT)n-SSR marker can be used for selective breeding for resistance to both viruses and bacteria in shrimp.
FRONTIERS IN GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Douyue Li, Saichao Pan, Hongxi Zhang, Yongzhuo Fu, Zhuli Peng, Liang Zhang, Shan Peng, Fei Xu, Hanrou Huang, Ruixue Shi, Heping Zheng, Yousong Peng, Zhongyang Tan
Summary: The study established 540 maps displaying the distributional features of SSRs in human Y-DNA at chromosomal level. The maps revealed significant SSR accumulation in certain bins, as well as sparse distributions in others, challenging the widely accepted view of SSR-rich regions in noncoding and intergenic regions of the Y-DNA. Furthermore, the study suggests biological significance and calls for further analysis of the huge number of SSRs in human Y-DNA.
Article
Plant Sciences
Luxian Liu, Yonghua Zhang, Pan Li
Summary: This study analyzed phylogenetic relationships within the Celtis genus and developed genome resources using genome skimming data from five Celtis species. The plastomes of Celtis showed a relatively conserved content and structure, with no signs of gene loss, translocations, inversions, or genome rearrangement. Additionally, plastid hotspot regions, polymorphic nuclear SSRs, and low or single-copy gene fragments were identified within Celtis.
Article
Agronomy
Nawel Malek, Meriem Miyassa Aci, Khalil Khamassi, Antonio Lupini, Mustapha Rouissi, Leila Hanifi-Mekliche
Summary: The study evaluated the genetic variability of 14 Algerian faba bean accessions through agro-morphological traits and SSR markers, finding significant phenotypic variation and genetic diversity among accessions. The accessions were not clustered based on geographical pattern, and most of the genetic variation was due to differentiation among accessions. Additionally, there was no substantial correlation between molecular and agro-morphological traits. These findings suggest the potential for establishment of faba bean breeding programs in Algeria with optimal agro- and molecular diversity.
Article
Entomology
Ali Milton, Dennis Muhanguzi, Allan Male, Ali Kajubi, Stephen Buah, Jerome Kubiriba, Robooni Tumuhimbise
Summary: This study aimed to develop a cheap and easily accessible genotyping method for the banana weevil. By using transcriptome-derived SSR markers, the genetic diversity of banana weevil populations in different regions of Uganda was analyzed, providing valuable information for integrated pest management strategies.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Meiqi Zhao, Guoping Shu, Yanhong Hu, Gangqiang Cao, Yibo Wang
Summary: This study used high-quality de novo-sequenced maize genomes to investigate the distribution and polymorphism of SSRs. The results showed that SSRs were more densely present in the telomeric region than the centromeric region, and were more abundant in genic sequences than intergenic sequences. The length and density of SSRs were found to be under the control of unknown evolutionary forces. The SSR polymorphism observed in different chromosome regions and motifs can be used as valuable genome resources for maize genetic research and molecular breeding.
Article
Plant Sciences
Moldir Yermagambetova, Shyryn Almerekova, Orzimat Turginov, Ormon Sultangaziev, Saule Abugalieva, Yerlan Turuspekov
Summary: The genetic diversity and population structure of 15 populations of Juniperus seravschanica from Central Asia were studied using 11 SSR markers. The results showed high genetic diversity and a relatively dispersed population structure, providing valuable data for future conservation strategies.