Article
Agronomy
Hsien-Tzu Wang, Yu-Jung Cheng, Jhih-Ting Hsiao, Fuu Sheu, Yen-Chou Kuan
Summary: This study investigated the expression of broccoli HSP and the effect of 17-AAG on broccoli senescence. Through RNA-sequencing, 11 HSP and related genes were identified, with 10 nM 17-AAG treatment significantly alleviating yellowing of broccoli and enhancing stress tolerance. This research highlights the potential of using HSP inducers like 17-AAG to improve shelf life and quality of postharvest crops.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Ophthalmology
Romain Touze, Marc M. Abitbol, Dominique Bremond-Gignac, Matthieu P. Robert
Summary: This study confirms the dysfunction of the retinal pigment epithelium (RPE) in patients with neurofibromatosis type 1 (NF1), characterized by a lower dark trough (DT) and a corresponding higher light peak (LP)/DT ratio. This pattern may be due to a dysregulation of melanocytogenesis, leading to disrupted calcium ion flux and abnormal polarization of the RPE.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Pharmacology & Pharmacy
Song Yang, Jun Zhou, Dengwen Li
Summary: The retinal pigment epithelium is crucial for visual functions, but damage to its structure and function can lead to various retinopathies with no cure currently available. Understanding the development, function, and pathobiology of the retinal pigment epithelium is essential for preventing and treating retinopathies. This review discusses potential strategies for treatment and provides new perspectives for future research in this field.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jyh-Cheng Chen, Jen-Chung Ko, Yong-Cing Taso, Hsiang-Hung Cheng, Tzu-Ying Chen, Ting-Chuan Yen, Yun-Wei Lin
Summary: The study revealed the regulatory effect of bevacizumab on XPC protein expression, and the synergistic cytotoxic effect of 17-AAG in combination with bevacizumab. These results provide a rationale for combining bevacizumab with Hsp90 inhibitors to enhance therapeutic effects for lung cancer.
Article
Materials Science, Multidisciplinary
Biola F. Egbowon, Enzo Fornari, Joseph M. Pally, Alan J. Hargreaves, Bob Stevens, T. Martin Mcginnity, Barbara K. Pierscionek
Summary: This study demonstrates that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without or with fluocinolone acetonide (FA) treatment. The cells survived and maintained their morphology for up to 150 days with FA-treated ENS, showing an epithelial phenotype with biomarkers critical for retinal characteristics. This technique has potential for subretinal transplantation.
MATERIALS & DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Jongmin Kim, Jae Yon Won
Summary: As the world ages, the number of age-related diseases, particularly those related to retinal pigment epithelium (RPE) degeneration, has increased. Photobiomodulation (PBM), a non-invasive treatment using red and near-infrared light, has shown potential as a new treatment for ocular diseases. Recent studies have demonstrated its antioxidant effect, making it a promising approach to cure RPE degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jeremie Canonica, Min Zhao, Tatiana Favez, Emmanuelle Gelize, Laurent Jonet, Laura Kowalczuk, Justine Guegan, Damien Le Menuet, Say Viengchareun, Marc Lombes, Eric Pussard, Yvan Arsenijevic, Francine Behar-Cohen
Summary: Glucocorticoids are commonly used drugs to treat retinal diseases, but the transcriptional regulations induced by GR and MR activation in RPE cells are not well understood. The study found that in the human eye, cortisol is the main ligand for GR and MR, and genes regulated by aldosterone and cortisol + RU-486 can mimic an imbalance toward MR activation. This activation in RPE cells may contribute to retinal pathology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Mohamed A. Faynus, Jeffrey K. Bailey, Britney O. Pennington, Mika Katsura, Duncan A. Proctor, Ashley K. Yeh, Sneha Menon, Dylan G. Choi, Jane S. Lebkowski, Lincoln Johnson, Dennis O. Clegg
Summary: Dry age-related macular degeneration (AMD) is a common eye disease with no current treatment options. Using microcarrier technology for cell culture allows efficient large-scale production of therapeutic retinal pigmented epithelial cells, fulfilling the treatment demands.
BIOENGINEERING-BASEL
(2022)
Article
Genetics & Heredity
Xiuxiu Jin, Jingyang Liu, Weiping Wang, Jiangfeng Li, Guangming Liu, Ruiqi Qiu, Mingzhu Yang, Meng Liu, Lin Yang, Xiaofeng Du, Bo Lei
Summary: This study analyzed the molecular changes of retinal pigment epithelium (RPE) cells during aging through quantitative proteomic analysis. The findings suggest that protein ubiquitination related to RNF123 and RNF149 plays a crucial role in protecting RPE cells from oxidative damage.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Masaaki Ishida, Sunao Sugita, Kenichi Makabe, Shota Fujii, Yoko Futatsugi, Hiroyuki Kamao, Suguru Yamasaki, Noriko Sakai, Akiko Maeda, Michiko Mandai, Masayo Takahashi
Summary: Research has shown that the addition of Y-27632 in vitro can suppress apoptosis, promote cell adhesion and proliferation, and increase the survival rate of transplants. In vivo experiments with human iPS-RPE cell transplantation into monkey eyes showed no obvious retinal toxicity caused by Y-27632.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
