期刊
MOLECULAR BIOLOGY REPORTS
卷 37, 期 4, 页码 2125-2133出版社
SPRINGER
DOI: 10.1007/s11033-009-9682-4
关键词
Plasmodium; snRNAs; 5 ' and 3 ' modifications; RNA caps; Adapter ligation RT-PCR
资金
- Department of Biotechnology, Govt. of India
- Wellcome Trust
- HHMI
- BIOTEC. PB
- Council for Scientific and Industrial Research
- University Grants Commission (Govt. of India)
5' caps provide recognition sequences for the nuclear import of snRNAs. The 5' and 3' ends of snRNAs were studied in Plasmodium falciparum with a modified adapter ligation method, which showed that 5' ends of U1, U2, U4, U5 and U6 snRNAs are capped. In P. falciparum, the 3' ends of U1, U2, U4 and U5 snRNAs have free hydroxyl groups whereas U6 snRNA has a blocked 3' end. An immunoprecipitation assay for trimethyl guanosine caps shows that the cap structures of parasite U1-U5 snRNAs are hypermethylated while U6 snRNA may be gamma-mono-methylated. Bioinformatics analysis of proteins involved in hypermethylation and trafficking of snRNAs indicates that the methyltransferase TGS1 is present in the P. falciparum genome. PfTGS1 is larger than its orthologs and may have transmembrane domains in the C-terminus. Surprisingly, the snRNA trafficking protein Snurportin is absent from the P. falciparum genome suggesting that reminiscent of yeast, parasite snRNAs may be retained in the nucleus.
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