期刊
MOLECULAR BIOLOGY REPORTS
卷 36, 期 6, 页码 1633-1636出版社
SPRINGER
DOI: 10.1007/s11033-008-9362-9
关键词
Arabidopsis thaliana; Genomic DNA; PCR; Protocol; Restriction enzymes
Protocols for genomic DNA extraction from plants are generally lengthily, since they require that tissues be ground in liquid nitrogen, followed by a precipitation step, washing and drying of the DNA pellet, etc. This represents a major challenge especially when several hundred samples must be screened/analyzed within a working day. There is therefore a need for a rapid and simple procedure, which will produce DNA quality suitable for various analyses. Here, we describe a time and cost efficient protocol for genomic DNA isolation from plants suitable for all routine genetic screening/analyses. The protocol is free from hazardous reagents and therefore safe to be executed by non-specialists. With this protocol more than 100 genomic DNA samples could manually be extracted within a working day, making it a promising alternative in genetic studies of large-scale genomic screening projects.
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