Article
Immunology
Satomi Mishima, Marin Sakamoto, Hikaru Kioka, Yuka Nagata, Ryo Suzuki
Summary: This study comprehensively examined the role of VAMP3 in mast cell function and the molecular mechanisms underlying it. The results suggest that VAMP3 mediates granule fusion and promotes granule exocytosis in mast cells. The loss of VAMP3 disrupts plasma membrane homeostasis and enhances Fc epsilon RI-mediated signal transduction. VAMP3 plays a multifunctional role in complex secretory responses in mast cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Xia Li, Dongmei Liu, Eric Griffis, Peter Novick
Summary: Bidirectional vesicular traffic is linked to both exocytic and endocytic pathways. Rab GTPases, including Sec4, are involved in determining the direction of vesicular transport. This study demonstrates that the directional specificity of Rab GTPase can be redirected, and that cells have a certain tolerance towards misdirection of Rab proteins.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Neurosciences
Yuuta Imoto, Sumana Raychaudhuri, Ye Ma, Pascal Fenske, Eduardo Sandoval, Kie Itoh, Eva-Maria Blumrich, Hideaki T. Matsubayashi, Lauren Mamer, Fereshteh Zarebidaki, Berit Sohl-Kielczynski, Thorsten Trimbuch, Shraddha Nayak, Janet H. Iwasa, Jian Liu, Bin Wu, Taekjip Ha, Takanari Inoue, Erik M. Jorgensen, Michael A. Cousin, Christian Rosenmund, Shigeki Watanabe
Summary: This study demonstrates that Dynamin 1 is pre-recruited to endocytic sites for ultrafast endocytosis. Syndapin 1 acts as an adaptor by binding the plasma membrane and storing Dynamin 1xA at endocytic sites, bypassing the recruitment step and accelerating endocytosis. These findings reveal the mechanism of endocytosis and provide new insights into cellular processes.
Article
Cell Biology
Nathan M. Willy, Joshua P. Ferguson, Ata Akatay, Scott Huber, Umidahan Djakbarova, Salih Silahli, Cemal Cakez, Farah Hasan, Henry C. Chang, Alex Travesset, Siyu Li, Roya Zandi, Dong Li, Eric Betzig, Emanuele Cocucci, Comert Kural
Summary: Research has shown that the footprint of clathrin coats increases during the formation of pits in endocytosis, while CALM adaptors form clusters and AP2 distribution is more homogeneous. These findings suggest that CALM clusters may increase strain on clathrin lattices, leading to rupture and completion of pits.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Sushila Kumari, Prasanta K. Dash, Tripti Kumari, Ming-Lei Guo, Jimut Kanti Ghosh, Shilpa J. Buch, Raj Kamal Tripathi
Summary: This study reveals that HIV-1 Nef differentially regulates the expression of Rab proteins in infected cells to manipulate host intracellular trafficking, enhancing viral replication and HIV-1 pathogenesis. The study emphasizes the essential role of HIV-1 protein Nef in various aspects of intracellular trafficking regulated by Rab GTPases, explaining how HIV-1 Nef hijacks membrane trafficking pathways in infected cells.
BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Ludek Koreny, Brandon N. Mercado-Saavedra, Christen M. Klinger, Konstantin Barylyuk, Simon Butterworth, Jennifer Hirst, Yolanda Rivera-Cuevas, Nathan R. Zaccai, Victoria J. C. Holzer, Andreas Klingl, Joel B. Dacks, Vern B. Carruthers, Margaret S. Robinson, Simon Gras, Ross F. Waller
Summary: Apicomplexan parasites have unique, stable endocytic structures that differ from other eukaryotes. These structures play a crucial role in maintaining plasma membrane homeostasis in these parasites. The origin of these specialized endocytic structures can be traced back to early adaptations in the complex cell pellicle of the infrakingdom Alveolata.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Peter Lonn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Bjorkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren
Summary: The study developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA), expanding the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bijeta Prasai, Gideon J. Haber, Marie-Paule Strub, Regina Ahn, John A. Ciemniecki, Kem A. Sochacki, Justin W. Taraska
Summary: By utilizing correlative super-resolution light and electron microscopy, researchers have mapped the location and organization of Rab-GTPases and their effectors on exocytic vesicles, shedding light on their functional roles within cells.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Mario Pinar, Miguel A. Penalva
Summary: RAB GTPases, as major determinants of membrane identity, have been widely used as specific reporters for studying intracellular traffic. Systematic studies on their localization and physiological role, particularly in Aspergillus nidulans, have influenced the field of intracellular trafficking beyond fungal specialists. These studies have revealed important roles of RABs in intracellular traffic.
MOLECULAR MICROBIOLOGY
(2021)
Article
Astronomy & Astrophysics
Olivia H. Wilkins, P. Brandon Carroll, Geoffrey A. Blake
Summary: Using high-angular-resolution observations with ALMA, we investigated the structure and variation of physical and chemical components in the Orion KL nebula on small spatial scales. We found that subregions of the Hot Core have higher excitation temperatures in smaller scales, indicating possible internal heating sources. Additionally, a bipolar feature with a southeast-northwest orientation was observed in the velocity field, suggesting the presence of an outflow. Evidence of a possible internal heating source toward the Northwest Clump was also identified. The Hot Core-SW region was identified as a particularly heterogeneous region bridging the Hot Core and Compact Ridge.
ASTROPHYSICAL JOURNAL
(2022)
Article
Astronomy & Astrophysics
Jun-Zhang Wang, Dian-Yong Chen, Xiang Liu, Takayuki Matsuki
Summary: The study introduces a Critical Energy induced Enhancement (CEE) mechanism for three-body open-charm processes in e(+)e(-) collisions, utilizing the unique kinematic behavior of e(+)e(-) annihilation involving three-body final states. By analyzing the process e(+)e(-) -> BC -> B(C -> DE), a reflection peak of an intermediate C-state near the invariant mass distribution threshold is found at the critical relation root s = m(B) + m(C), suggesting the presence of charmoniumlike structures. Recommendations are made for optimal center-of-mass energy points for exploring new charmoniumlike structures in three-body open-charm processes, accessible at BESIII and BelleII.
Review
Environmental Sciences
Mishkah Abrahams, Mbulisi Sibanda, Timothy Dube, Vimbayi G. P. Chimonyo, Tafadzwanashe Mabhaudhi
Summary: This study systematically reviewed the literature on the remote sensing of the spatial distribution and health of neglected and underutilised crop species (NUS). The findings showed slow progress in using unmanned aerial vehicles (UAVs) for mapping NUS, particularly in the Global South, due to high costs and restrictive regulations.
Article
Nanoscience & Nanotechnology
Evan Lammertse, Nikhil Koditala, Martin Sauzade, Hongxiao Li, Qiang Li, Luc Anis, Jun Kong, Eric Brouzes
Summary: This research presents a simple defocusing approach based on brightfield microscopy and open-source software to map micro-flows in 3D at high spatial and temporal resolution. The method is validated on three microfluidic examples, revealing novel structures and patterns in displacement structures and droplet microfluidics.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Mathematics, Interdisciplinary Applications
Jose Balsa-Barreiro, Alfredo J. Morales, Ruben C. Lois-Gonzalez
Summary: Currently, around half of the global population resides in urban areas, a figure expected to rise to two-thirds by 2050. While many studies focus on urban dynamics, it is crucial to analyze population distribution at different spatial scales.
Article
Biotechnology & Applied Microbiology
Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima, Archibald Enninful, Xiaoyu Qin, Yanxiang Deng, Jungmin Nam, Fu Gao, Pino Bordignon, Marco Cassano, Mary Tomayko, Mina Xu, Stephanie Halene, Joseph E. E. Craft, David Hafler, Rong Fan
Summary: In this study, the researchers extended the co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and successfully mapped a large number of proteins and the whole transcriptome. They applied this method to analyze multiple mouse tissue types and human tissues, revealing spatially distinct reactions and immune activation. The co-indexing of transcriptomes and epitopes in the spatial dimension with large protein panels proved to be effective.
NATURE BIOTECHNOLOGY
(2023)
Review
Cell Biology
Claudia Matthaeus, Justin W. Taraska
Summary: Caveolae are plasma membrane invaginations with a diameter of 70-100 nm, abundant in specific cell types and characterized by specific lipid binding proteins. They provide support for proteins involved in cell-specific signaling pathways and play an important role in lipid trafficking and metabolism through their ability to detach from the plasma membrane and move through the cytosol.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Physics, Multidisciplinary
Nicolas T. Chartier, Arghyadip Mukherjee, Julia Pfanzelter, Sebastian Furthauer, Ben T. Larson, Anatol W. Fritsch, Rana Amini, Moritz Kreysing, Frank Julicher, Stephan W. Grill
Summary: In the nematode Caenorhabditis elegans, the decision between life and death in the germline is determined by a hydraulic symmetry-breaking transition, where some germ cells grow while others shrink due to a hydraulic instability amplifying volume differences. This phenomenon is linked to the mechanical cell-fate decision and tissue hydraulics. The shrinking germ cells are extruded and die, demonstrating the importance of tissue hydraulics in cell development.
Article
Multidisciplinary Sciences
Bijeta Prasai, Gideon J. Haber, Marie-Paule Strub, Regina Ahn, John A. Ciemniecki, Kem A. Sochacki, Justin W. Taraska
Summary: By utilizing correlative super-resolution light and electron microscopy, researchers have mapped the location and organization of Rab-GTPases and their effectors on exocytic vesicles, shedding light on their functional roles within cells.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Marco A. Alfonzo-Mendez, Kem A. Sochacki, Marie-Paule Strub, Justin W. Taraska
Summary: The study reveals the interaction between growth factor signaling and cell adhesion, and highlights the role of flat clathrin lattices in enhancing signal transduction.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Ruthellen H. Anderson, Kem A. Sochacki, Harika Vuppula, Brandon L. Scott, Elizabeth M. Bailey, Maycie M. Schultz, Jason G. Kerkvliet, Justin W. Taraska, Adam D. Hoppe, Kevin R. Francis
Summary: Clathrin-mediated endocytosis (CME) is crucial for cellular signal transduction, receptor recycling, and membrane homeostasis, while cholesterol depletion can disrupt CME; sterol structural requirements play an important role in efficient CME, and reduced cholesterol levels may contribute to defects in CME-mediated substance internalization.
Correction
Cell Biology
Ruthellen H. Anderson, Kem A. Sochacki, Harika Vuppula, Brandon L. Scott, Elizabeth M. Bailey, Maycie M. Schultz, Jason G. Kerkvliet, Justin W. Taraska, Adam D. Hoppe, Kevin R. Francis
Article
Multidisciplinary Sciences
Gianvito Arpino, Agile Somasundaram, Wonchul Shin, Lihao Ge, Seth Villareal, Chung Yu Chan, Uri Ashery, Oleg Shupliakov, Justin W. Taraska, Ling-Gang Wu
Summary: Clathrin-mediated endocytosis collaborates closely with bulk endocytosis in creating invaginations to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance in various cell types throughout the body.
Meeting Abstract
Biochemistry & Molecular Biology
Claudia Matthaeus, Kem Sochacki, Dmytro Puchkov, Volker Haucke, Martin Lehmann, Justin Taraska
Review
Cell Biology
Michael M. Kozlov, Justin W. Taraska
Summary: Membrane curvature is crucial for the formation of membrane structures and plays a key role in membrane trafficking. Protein-based mechanisms, organized by protein coats, are involved in membrane bending. The generation of curved membranes requires dynamic protein complexes and relies on fundamental mechanisms such as hydrophobic insertion, scaffolding, and crowding. Coats, such as clathrin, caveolar coat complex, COPI and COPII coats, integrate and coordinate curvature-generating factors to shape membranes for intracellular organelles and vesicular structures. This dynamic process is essential for membrane trafficking within cells.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Liu Yang, Margret A. Fye, Bingyuan Yang, Zihan Tang, Yue Zhang, Sander Haigh, Brittney A. Covington, Kai Bracey, Justin W. Taraska, Irina Kaverina, Shen Qu, Wenbiao Chen
Summary: Through a genome-wide CRISPR/Cas9 knockout screen, we identified members of the COMMD family as positive regulators of basal insulin secretion. They enhance basal insulin secretion by promoting the docking of insulin granules to the plasma membrane through ITGB1 recycling.
MOLECULAR METABOLISM
(2022)
Article
Multidisciplinary Sciences
Claudia Matthaeus, Kem A. Sochacki, Andrea M. Dickey, Dmytro Puchkov, Volker Haucke, Martin Lehmann, Justin W. Taraska
Summary: This study investigates the key proteins that regulate the curvature of caveolae using correlative super-resolution light and electron microscopy. The authors discover that caveolins, cavins, and EHD2 form a cohesive structural unit, regulated by intermittent associations with Pacsin2 and EHBP1, to control the curvature of caveolae.
NATURE COMMUNICATIONS
(2022)
Article
Zoology
Ben T. Larson
Summary: Cells are the basic building blocks of biological organization and possess remarkable complexity in their behaviors. By studying protists from the perspective of cellular biophysics, we can gain insights into general principles of morphogenesis, navigation and motility, and decision making. Applying biophysical approaches to evolutionary cell biology offers exciting opportunities, as integrating and expanding biophysical studies across protist diversity will enhance our understanding of underlying principles.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2023)
Meeting Abstract
Biophysics
Kazuki Obashi, Kem A. Sochacki, Marie-Paule Strub, Justin W. Taraska
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Bijeta Prasai, Gideon Haber, John Ciemniecki, Marie-Paule Strub, Kem A. Sochacki, Justin W. Taraska
BIOPHYSICAL JOURNAL
(2021)
Meeting Abstract
Biophysics
John Jimah, Nidhi Kundu, Kem A. Sochacki, Abigail Stanton, Lieza M. Chan, Justin W. Taraska, Jenny E. Hinshaw
BIOPHYSICAL JOURNAL
(2021)