Article
Food Science & Technology
Nancy Chasquibol, Billy Francisco Gonzales, Rafael Alarcon, Axel Sotelo, Jose Carlos Marquez-Lopez, Noelia M. Rodriguez-Martin, Maria del Carmen Millan-Linares, Francisco Millan, Justo Pedroche
Summary: In this research, the proximal composition, protein solubility, and amino acid profile of scallop (Argopecten purpuratus) visceral meal (SVM) and defatted meal (SVMD) were analyzed. The hydrolyzed proteins isolated from scallop's viscera (SPH) were optimized and characterized using response surface methodology. The optimized protein hydrolysates showed high yield and degree of hydrolysis, and the amino acid composition was balanced and suitable for healthy nutrition.
Article
Multidisciplinary Sciences
Yuan Ren, Jie Yang, Barbara Fujita, Huaizhou Jin, Yongli Zhang, Julien Berro
Summary: Forces play important roles in various cellular processes, but it is challenging to measure them at the molecular scale in vivo. In this study, new in vivo force sensors were used to measure the forces on the protein End4p at different locations, revealing their redistribution across the endocytic machinery.
Article
Multidisciplinary Sciences
Feng Yuan, Haleh Alimohamadi, Brandon Bakka, Andrea N. Trementozzi, Kasey J. Day, Nicolas L. Fawzi, Padmini Rangamani, Jeanne C. Stachowiak
Summary: "Membrane bending is primarily driven by proteins with specific structural features, such as amphipathic helices and crescent-shaped scaffolds. Recent studies show that disordered protein domains can form liquid phases on membrane surfaces, creating compressive stress that leads to membrane curvature and the formation of protein-lined membrane tubules."
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Rahul Bhattacharjee, Aaron R. Hall, MariaSanta C. Mangione, Maya G. Igarashi, Rachel H. Roberts-Galbraith, Jun-Song Chen, Dimitrios Vavylonis, Kathleen L. Gould
Summary: The F-BAR protein Cdc15 is crucial for cytokinesis in yeast and is involved in attaching the cytokinetic ring to the plasma membrane. Various cell polarity kinases, particularly Pom1, regulate the phosphorylation of Cdc15, which in turn affects its localization and ability to interact with the membrane. Coarse-grained simulations suggest that phosphorylation prevents F-BAR oligomerization and membrane interaction, and dephosphorylated Cdc15 undergoes liquid-liquid phase separation to form condensates.
Article
Multidisciplinary Sciences
Kazuya Tsujita, Reiko Satow, Shinobu Asada, Yoshikazu Nakamura, Luis Arnes, Keisuke Sako, Yasuyuki Fujita, Kiyoko Fukami, Toshiki Itoh
Summary: Changes in cell mechanics contribute to cancer cell dissemination. High plasma membrane (PM) tension inhibits cancer dissemination by counteracting mechanosensitive BAR family protein assembly, while restoration of PM tension phenotypically convert malignant cells into a non-motile epithelial cell state.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Hossein Moghimianavval, Chintan Patel, Sonisilpa Mohapatra, Sung-Won Hwang, Tunc Kayikcioglu, Yashar Bashirzadeh, Allen P. Liu, Taekjip Ha
Summary: InterSpy is introduced as a synthetic biology tool for engineering membrane-membrane interfaces. It allows tracking and reconstitution of functional fluorescent protein between apposing synthetic or cell membranes, demonstrating the potential for designing non-native cellular communication pathways and creating synthetic tissues. The technology is tested using a mammalian cell-free expression system and shows promising results.
Article
Biochemistry & Molecular Biology
Madhumalar Subramanian, Charlotte Kielar, Satoru Tsushima, Karim Fahmy, Jana Oertel
Summary: The use of engineered cysteines on membrane-scaffolding protein MSP1D1 allows the formation of multimers of nanodiscs, which is crucial for membrane protein research. Minimal interference with nanodisc formation and structure was demonstrated by various analytical techniques. Transmission electron microscopy showed the growth of submicron-sized tracts of stacked nanodiscs through oligonucleotide attachment to engineered cysteines.
Article
Cell Biology
Joshua Okletey, Dimitrios Angelis, Tia M. Jones, Cristina Montagna, Elias T. Spiliotis
Summary: This study identifies SEPT9_i1 as a component of breast cancer invadopodia, which play a role in the interaction between the nuclear envelope and the extracellular matrix (ECM) degrading structures. Depletion of SEPT9_i1 reduces invadopodium formation and leads to deformed nuclei. The plasticity of the nucleus is believed to be associated with cancer cell invasion.
Article
Multidisciplinary Sciences
Kenta Shigetomi, Yumiko Ono, Kenji Matsuzawa, Junichi Ikenouchi
Summary: It has been found that cholesterol accumulation is essential for the formation of tight junctions, which are crucial for the epithelial barrier. Despite the absence of tight junctions, cholesterol still accumulates normally in the vicinity of the apical junctions. Moreover, a claudin mutant that cannot bind to Zonula Occludens (ZO) proteins can still form tight junction strands. ZO proteins are not only scaffolds for claudins, but also promote the formation of cholesterol-rich membrane domains at apical junctions through their effect on the junctional actomyosin cytoskeleton.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Jun Zhang, Jennifer F. Pinello, Ignacio Fernandez, Eduard Baquero, Juliette Fedry, Felix A. Rey, William J. Snell
Summary: Recognition and fusion between gametes during fertilization is an ancient process. Protein HAP2, a structural homolog of viral class II fusion proteins, undergoes an obligate conformational rearrangement on minus gametes for successful fusion in Chlamydomonas. This rearrangement is triggered only by species-specific adhesion between the two gamete membranes.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Steven J. Del Signore, Charlotte F. Kelley, Emily M. Messelaar, Tania Lemos, Michelle F. Marchan, Biljana Ermanoska, Markus Mund, Thomas G. Fai, Marko Kaksonen, Avital Adah Rodal
Summary: This study reveals a mechanism by which autoinhibition limits actin assembly of presynaptic endocytic machinery, promoting membrane-coupled productive endocytosis. Interaction between Drosophila endocytic proteins helps to constrain actin dynamics at synapses and drive efficient membrane remodeling in response to physiological cues.
Article
Microbiology
Wei He, Gangjin Yu, Tianpeng Li, Ling Bai, Yuanyuan Yang, Zixiao Xue, Yonghao Pang, Dana Reichmann, Sebastian Hiller, Lichun He, Maili Liu, Shu Quan
Summary: Our research demonstrates the role of the periplasmic chaperone Spy in maintaining the homeostasis of certain outer membrane proteins (OMPs). Spy utilizes a unique chaperone mechanism to bind OmpX, allowing it to form a partially folded beta-strand secondary structure in a dynamic exchange of conformations. This mechanism differs from that of other E. coli periplasmic chaperones such as Skp and SurA, highlighting the differences in the mechanisms of ATP-independent chaperones.
Review
Biochemistry & Molecular Biology
Magdalena Kulma, Gregor Anderluh
Summary: Pore-forming proteins (PFPs) alter membrane permeability by forming transmembrane pores, destroying target cells and potentially manipulating cellular functions. Recent evidence suggests that PFPs also induce extensive membrane reorganization, including lateral rearrangement and deformation, which can disrupt cell function and lead to death. These modifications are also part of a membrane repair system that protects cells from the lethal effects of pore formation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Shinya Yuge, Koichi Nishiyama, Yuichiro Arima, Yasuyuki Hanada, Eri Oguri-Nakamura, Sanshiro Hanada, Tomohiro Ishii, Yuki Wakayama, Urara Hasegawa, Kazuya Tsujita, Ryuji Yokokawa, Takashi Miura, Toshiki Itoh, Kenichi Tsujita, Naoki Mochizuki, Shigetomo Fukuhara
Summary: Blood flow-driven intraluminal pressure (IP) plays a crucial role in regulating wound angiogenesis. F-BAR proteins TOCA1 and CIP4 are key actin regulatory proteins that control endothelial cell migration and sense mechanical cell stretching to regulate wound angiogenesis.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Chemical
Yufeng Song, Lixin Feng, Sagnik Basuray, Kamalesh K. Sirkar, S. R. Wickramasinghe
Summary: The study investigated the separation and purification of a binary protein mixture of hemoglobin and bovine serum albumin using ultrafiltration membranes. It demonstrated that the internally staged ultrafiltration (ISUF) technique with adjusted pH and low applied pressure difference can effectively prevent membrane fouling and achieve high purity of the proteins. The results suggest that the ISUF technique may be applicable for practical protein separation and purification systems.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kim Adameyko, Anton Burakov, Alexander D. Finoshin, Kirill Mikhailov, Oksana Kravchuk, Olga S. Kozlova, Nicolay G. Gornostaev, Alexander Cherkasov, Pavel A. Erokhov, Maria I. Indeykina, Anna E. Bugrova, Alexey S. Kononikhin, Andrey Moiseenko, Olga S. Sokolova, Artem N. Bonchuk, Irina Zhegalova, Anton A. Georgiev, Victor S. Mikhailov, Natalia E. Gogoleva, Guzel R. Gazizova, Elena Shagimardanova, Oleg A. Gusev, Yulia Lyupina
Summary: Ferritins are a conservative family of proteins found in all species, playing essential roles in resistance to redox stress, immune response, and cell differentiation. The study characterized ferritins in two cold-water sponges, revealing their regulation by cellular iron and oxygen levels, and their importance in various biological processes. Further research is needed to explore the function of atypical ferritins lacking iron-binding capacity in invertebrate species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Rylie B. Walsh, Erica C. Dresselhaus, Agata N. Becalska, Matthew J. Zunitch, Cassandra R. Blanchette, Amy L. Scalera, Tania Lemos, So Min Lee, Julia Apiki, ShiYu Wang, Berith Isaac, Anna Yeh, Kate Koles, Avital A. Rodal
Summary: The loss of the endosomal retromer complex in Drosophila motor neurons leads to accumulation of cargoes in neuronal extracellular vesicles (EVs), while mutations in regulators of endosome-plasma membrane recycling such as rab11 and rab4 cause reduced EV cargo levels. The study suggests that EV traffic is influenced by a balance between Rab4/Rab11 recycling and retromer-dependent removal from EV precursor compartments, shedding light on competing pathways in Alzheimer's disease and the potential role of misregulated EV traffic as an underlying defect.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Anatomy & Morphology
Dmitry Bagrov, Grigory S. Glukhov, Andrey Moiseenko, Maria G. Karlova, Daniil S. Litvinov, Petr A. Zaitsev, Liubov Kozlovskaya, Anna A. Shishova, Anastasia A. Kovpak, Yury Y. Ivin, Anastasia N. Piniaeva, Alexey S. Oksanich, Viktor P. Volok, Dmitry Osolodkin, Aydar A. Ishmukhametov, Alexey M. Egorov, Konstantin Shaitan, Mikhail P. Kirpichnikov, Olga S. Sokolova
Summary: This study characterized the beta-propiolactone inactivated SARS-CoV-2 virions using TEM and AFM, finding that samples prepared using SEC and IEC retained more spikes on the surface and the S proteins were in the pre-fusion conformation. Analytical TEM showed that the inactivated virions retained nucleic acid, demonstrating their potential as a vaccine candidate.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Plant Sciences
Ethan Weiner, Justine M. Pinskey, Daniela Nicastro, Marisa S. Otegui
Summary: Recent developments in instrumentation and image analysis algorithms have advanced the capabilities of 3D-EM, allowing for automated image collections and near-atomic resolution imaging of macromolecular complexes. This review focuses on the applications of cryo-ET in plant and algal cell biology research, highlighting its unique ability to reveal the organization of eukaryotic organelles. Challenges in sample preparation for cryo-ET are also discussed, along with the development of correlative light and electron microscopy approaches.
Correction
Crystallography
I. S. Panina, A. A. Mamchur, I. A. Yaroshevich, D. V. Zlenko, E. B. Pichkur, S. S. Kudryavtseva, V. I. Muronetz, O. S. Sokolova, T. B. Stanishneva-Konovalova
CRYSTALLOGRAPHY REPORTS
(2022)
Article
Multidisciplinary Sciences
Yanhe Zhao, Huafeng Wang, Caroline Wiesehoefer, Naman B. Shah, Evan Reetz, Jae Yeon Hwang, Xiaofang Huang, Tse-en Wang, Polina V. Lishko, Karen M. Davies, Gunther Wennemuth, Daniela Nicastro, Jean-Ju Chung
Summary: The study visualizes the native in-cell 3D structure and higher-order organization of the CatSper calcium channels using cryo-electron tomography. The authors discover that the CatSper channels form long zigzag rows along the sperm tail and interact with other molecules. Moreover, the loss of EFCAB9-CATSPER zeta alters the architecture and interactions of the channels, disrupting flagellar movement in sperm.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Sean Rogers, Long Gui, Anastasiia Kovalenko, Valeria Zoni, Maxime Carpentier, Kamran Ramji, Kalthoum Ben Mbarek, Amelie Bacle, Patrick Fuchs, Pablo Campomanes, Evan Reetz, Natalie Ortiz Speer, Emma Reynolds, Abdou Rachid Thiam, Stefano Vanni, Daniela Nicastro, W. Mike Henne
Summary: The study reveals that yeast glucose restriction can induce liquid crystalline phase transitions and lipid demixing in lipid droplets, leading to selective remodeling of their proteome.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Olga S. Sokolova, Evgeny B. Pichkur, Ekaterina S. Maslova, Lidia P. Kurochkina, Pavel Semenyuk, Petr Konarev, Valeriya R. Samygina, Tatiana B. Stanishneva-Konovalova
Summary: Chaperonins are a family of molecular chaperones that assist in protein folding. They are categorized into two groups, and a potential new group has recently been identified in giant bacteriophages. Using cryo-EM, researchers determined the structure of a new chaperonin encoded by gene 228 of phage AR9 B. subtilis, revealing similarities and differences with known chaperonins.
Article
Biology
Justine M. Pinskey, Adhya Lagisetty, Long Gui, Nhan Phan, Evan Reetz, Amirrasoul Tavakoli, Gang Fu, Daniela Nicastro
Summary: This study used cryo-electron microscopy to investigate flagella in the aquatic eukaryote choanoflagellates, and found that their flagellar structures are more similar to those of multicellular organisms. Additionally, unique features of choanoflagellate flagella were described, and unknown functional structures were reported on the extracellular surface of the flagellar membrane.
Article
Biochemistry & Molecular Biology
Aleksandra A. Mamchur, Tatiana B. Stanishneva-Konovalova, Yuliana A. Mokrushina, Viktoria A. Abrikosova, Yu Guo, Hongkai Zhang, Stanislav S. Terekhov, Ivan V. Smirnov, Igor A. Yaroshevich
Summary: Researchers explored a computational approach based on molecular dynamics simulations and conformational clusterization techniques to study the wild-type and omicron variants of RBD of SARS-CoV-2 spike protein. Biochemical experiments supported the presence of multiple conformational states within the RBD assembly. The development of such an approach will aid in selecting neutralization drugs with higher affinity based on the primary structure of the target antigen.
Article
Crystallography
I. S. Panina, A. A. Mamchur, I. A. Yaroshevich, D. V. Zlenko, E. B. Pichkur, S. S. Kudryavtseva, V. I. Muronetz, O. S. Sokolova, T. B. Stanishneva-Konovalova
Summary: The three-dimensional reconstruction of GroEL apo-form obtained by cryo-EM and MD calculations reveals a diverse mobility of the protein subunit domains, providing important insights into the mechanism of protein folding in the GroEL cavity.
CRYSTALLOGRAPHY REPORTS
(2021)
Article
Crystallography
E. B. Pichkur, V. I. Mikirtumov, O. V. Tikhonova, N. I. Derkacheva, L. P. Kurochkina, O. S. Sokolova
Summary: The roughly purified extract of E. coli proteins has been studied by cryoelectron microscopy, and the 2D projections of two proteins have been distinguished, identified in the extract by tandem mass spectrometry. The structures of these proteins have been solved at near-atomic resolution, with de novo simulation revealing differences in the positions of some amino acid residues in the active center compared to known crystal structures.
CRYSTALLOGRAPHY REPORTS
(2021)
Article
Immunology
Liubov Kozlovskaya, Anastasia N. Piniaeva, Georgy M. Ignatyev, Ilya Gordeychuk, Viktor P. Volok, Yulia Rogova, Anna A. Shishova, Anastasia A. Kovpak, Yury Yu Ivin, Liliya P. Antonova, Kirill M. Mefyod, Lyubov S. Prokosheva, Anna S. Sibirkina, Yuliya Yu Tarasova, Ekaterina O. Bayurova, Olga S. Gancharova, Victoria V. Illarionova, Grigory S. Glukhov, Olga S. Sokolova, Konstantin Shaitan, Anastasia M. Moysenovich, Stanislav A. Gulyaev, Tatiana Gulyaeva, Andrey Moroz, Larissa Gmyl, Elena G. Ipatova, Mikhail P. Kirpichnikov, Alexey M. Egorov, Aleksandra A. Siniugina, Aydar A. Ishmukhametov
Summary: The CoviVac vaccine showed good safety, protective efficacy, and immunogenicity in animal models, providing encouraging data for further evaluation in clinical trials.
EMERGING MICROBES & INFECTIONS
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
O. Volokh, A. Sivkina, M. Karlova, E. Kotova, V. Studitsky, O. S. Sokolova
Meeting Abstract
Biochemistry & Molecular Biology
Y. Kacher, M. Karlova, V. Rusinova, D. Abramochkin, E. Zaklyazminskaya, O. S. Sokolova