期刊
MOLECULAR BIOLOGY OF THE CELL
卷 24, 期 18, 页码 2807-2819出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-05-0229
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资金
- Ruth L. Kirschstein National Research Service Award Fellowship, a Williston Postdoctoral Fellowship [F32 AI800742]
- American Cancer Society [PF-12-108-01-CCG]
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [AI075096]
- Pew Charitable Trusts through the Pew Scholars Program in the Biomedical Sciences
- National Institutes of Health [GM100122]
The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore-microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore-microtubule copy number from one to more than one kinetochore-microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.
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