Article
Biochemistry & Molecular Biology
Beth A. Griesel, Satoshi Matsuzaki, Albert Batushansky, Timothy M. Griffin, Kenneth M. Humphries, Ann Louise Olson
Summary: This study using 3T3-L1 adipocytes as a model system found that an increase in glucose uptake during the first 3 days of differentiation promoted the expansion of the adipocyte metabolome and proteome. The positive effects were specifically due to increased expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), highlighting its critical role in regulating cellular metabolic remodeling for adipocyte differentiation and maturation.
Article
Biochemistry & Molecular Biology
Yue Li, Weidong Wang, Hui-Ying Lim
Summary: Through genetic studies, it was found that the regulation of glucose absorption in the Drosophila midgut is mediated by the Solute Carrier 5A5 (dSLC5A5). Genetically knocking down dSLC5A5 in flies leads to decreased glucose and trehalose levels in the system and circulation, while overexpression of dSLC5A5 increases these levels and promotes glucose uptake in enterocytes. Additionally, it was shown that dSLC5A5 undergoes apical endocytosis in a dynamin-dependent manner, crucial for glucose uptake in enterocytes, and that glucose upregulates dSLC5A5 levels in the midgut, influencing systemic glucose homeostasis on a high-sugar diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Edoardo Moretto, Federico Miozzo, Anna Longatti, Caroline Bonnet, Francoise Coussen, Fanny Jaudon, Lorenzo A. Cingolani, Maria Passafaro
Summary: In rat hippocampal neurons, the tetraspanin TSPAN5 was found to promote exocytosis of AMPA receptors without affecting their internalisation. This function is mediated by TSPAN5 interacting with the adaptor protein complex AP4 and Stargazin, possibly using recycling endosomes as a delivery route. This study highlights the importance of TSPAN5 as a new adaptor protein regulating AMPA receptor trafficking.
Article
Biochemistry & Molecular Biology
Tomomi Minemura, Atsunori Fukuhara, Michio Otsuki, Iichiro Shimomura
Summary: The study reveals that LDHA regulates Glut1 expression and basal glucose uptake in adipocytes. Knockout or inhibition of LDHA leads to decreased Glut1 protein levels and reduced glucose uptake.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Monira Obaid, S. M. Nashir Udden, Prasanna Alluri, Subhrangsu S. Mandal
Summary: The study demonstrates that during inflammation, HOTAIR plays a key role in regulating Glut1 expression and glucose uptake by activating NF-kB, thereby controlling metabolic programming in immune cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Konstantina Amoiradaki, Kate R. Bunting, Katherine M. Paine, Josephine E. Ayre, Karen Hogg, Kamilla M. E. Laidlaw, Chris MacDonald
Summary: The study shows that multiple subunits of the Rpd3 lysine deacetylase complex play a crucial role in the recycling of surface proteins, with potential downstream target genes identified through validation and bioinformatics analysis. This suggests that recycling is controlled by Rpd3 at the transcriptional level through multiple downstream target genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hanh M. Truong, Kevin O. Cruz-Colon, Jorge Y. Martinez-Marquez, Jason R. Willer, Amanda M. Travis, Sondip K. Biswas, Woo-Kuen Lo, Hanno J. Bolz, Jillian N. Pearring
Summary: The tectonic complex acts as a barrier in the photoreceptor cilium to prevent unsolicited protein entry, ensuring proper functioning of the photoreceptor.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Ying Liu, Kejia Liu, Rick F. Thorne, Ronghua Shi, Qingyuan Zhang, Mian Wu, Lianxin Liu
Summary: This article investigates the role of SUMOylation and deSUMOylation of Succinate dehydrogenase (SDH) in regulating cellular metabolism. The study reveals that SDHA is SUMOylated by PIAS3 and deSUMOylated by SENP2, which affects the assembly and activity of the SDH complex. Additionally, acetylation of SENP2 by CBP negatively regulates its deSUMOylation activity, thereby regulating the TCA cycle and electron transport chain activity. This mechanism avoids excessive ROS production and promotes cancer cell survival.
Article
Cardiac & Cardiovascular Systems
Kim A. Connelly, C. David Mazer, Pankaj Puar, Hwee Teoh, Chao-Hung Wang, Tamique Mason, Farhad Akhavein, Ching-Wen Chang, Min-Hui Liu, Ning- Yang, Wei-Siang Chen, Yu-Hsiang Juan, Erika Opingari, Yaseen Salyani, William Barbour, Aryan Pasricha, Shamon Ahmed, Andrew Kosmopoulos, Raj Verma, Michael Moroney, Ehab Bakbak, Aishwarya Krishnaraj, Deepak L. Bhatt, Javed Butler, Mikhail N. Kosiborod, Carolyn S. P. Lam, David A. Hess, Otavio Rizzi Coelho-Filho, Myriam Lafreniere-Roula, Kevin E. Thorpe, Adrian Quan, Lawrence A. Leiter, Andrew T. Yan, Subodh Verma
Summary: This study aimed to evaluate the effect of sodium-glucose cotransporter 2 inhibition with empagliflozin on left ventricular mass in individuals without diabetes or significant heart failure. The results showed that after 6 months of treatment with empagliflozin, there was no significant reduction in left ventricular mass index. The findings suggest that empagliflozin may not have meaningful benefits on cardiac remodeling in this population.
Article
Pharmacology & Pharmacy
Serika Motoike, Kei Taguchi, Kana Harada, Masaya Asano, Izumi Hide, Shigeru Tanaka, Masahiro Irifune, Norio Sakai
Summary: The study revealed a functional association between SERT and STX3, showing that STX3 overexpression can reduce SERT uptake activity. STX3 knockdown affects SERT glycosylation, while in naturally expressing cells like Caco-2, STX3 positively regulates SERT function.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2021)
Article
Developmental Biology
Kumari Pushpa, Sunayana Dagar, Harsh Kumar, Diksha Pathak, Sivaram V. S. Mylavarapu
Summary: The study demonstrates that the exocyst complex regulates germline stem cell proliferation by modulating Notch signaling autonomously in the nematode Caenorhabditis elegans. Additionally, the exocyst complex interacts with other cellular components to maintain optimal membrane Notch levels and is required for Notch plasma membrane localization and signaling in mammalian cells.
Article
Cell Biology
Minhee Kim, Jingjing Lin, Jeong-Eun Huh, Jin Hee Park, Miyeon Go, Hana Lee, Donghyun Hwang, Han Sung Kim, Taesoo Kim, Daekee Lee, Soo Young Lee
Summary: The study revealed that TSPAN7 plays a crucial role in regulating the bone-resorbing function of osteoclasts by modulating actin ring formation. Deletion of Tm4sf2 led to significant defects in integrin-mediated actin ring formation, resulting in decreased bone resorption. The findings suggest that TSPAN7 acts as a novel modulator in osteoclasts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Developmental Biology
Yue Li, Weidong Wang, Hui-Ying Lim
Summary: The availability of glucose transporter in the small intestine is crucial for glucose uptake and systemic glucose homeostasis. Research on the molecular mechanisms of sodium-glucose linked transporter 1 (SGLT1) regulation in the small intestine is incomplete. This study identified a gene called dTMEM214 that regulates glucose homeostasis and uptake in the midgut of Drosophila. dTMEM214 regulates the subcellular distribution of another protein, dSLC5A5, in the enterocytes, thereby acting as a molecular regulator of glucose transporter in the midgut.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Shuai Ma, Wenzhu Yang, Xiaoqing Liu, Suzhen Li, Ye Li, Jiameng Zhu, Chunyi Zhang, Xiaoduo Lu, Xiaojin Zhou, Rumei Chen
Summary: A newly identified PPR protein CNS1 regulates the 5'-terminal integrity of mitochondrial RNA, affecting the function of mitochondrial complex III and seed development in maize.
Article
Pathology
Chuandong Cheng, Junhong Tu, Zengchun Hu, Yinan Chen, Yue Wang, Ting Zhang, Caihua Zhang, Cong Li, Yang Wang, Chaoshi Niu
Summary: Cholesterol plays a vital role in glioblastoma multiforme (GBM), and its synthesis is regulated by SREBP2, while GLUTs and Rab11s are also involved. The study found that high expression of SREBP2, GLUT1, and GLUT6 is associated with poor prognosis in GBM patients, and SREBP2 and Rab11s regulate GLUT1 and GLUT6, affecting cell viability and migration. The study suggests that the SREBP2/Rab11/GLUT network promotes T98G cell growth, providing potential therapeutic targets for GBM.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Biographical-Item
Urology & Nephrology
Dontscho Kerjaschki, Yashpal Kanwar, Robert A. Orlando, Sanna Lehtonen, Aaro Miettinen
KIDNEY INTERNATIONAL
(2020)
Article
Chemistry, Multidisciplinary
Mika E. A. Berg, Jette-Britt Naams, Laura C. Hautala, Tuomas A. Tolvanen, Jari P. Ahonen, Sanna Lehtonen, Kristiina Wahala
Article
Endocrinology & Metabolism
Hong Wang, Sara Kuusela, Rita Rinnankoski-Tuikka, Vincent Dumont, Rim Bouslama, Usama Abo Ramadan, Jo Waaler, Anni-Maija Linden, Nai-Wen Chi, Stefan Krauss, Eija Pirinen, Sanna Lehtone
INTERNATIONAL JOURNAL OF OBESITY
(2020)
Article
Pathology
Anna Kaisa Kylma, Tuomas Aleksi Tolvanen, Timo Carpen, Caj Haglund, Antti Makitie, Petri S. Mattila, Reidar Grenman, Lauri Jouhi, Timo Sorsa, Sanna Lehtonen, Jaana Hagstrom
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Victoria Ortin-Piqueras, Tobias L. Freitag, Leif C. Andersson, Sanna H. Lehtonen, Seppo K. Meri, Thomas Spillmann, Rafael Frias
Summary: Intestinal permeability tests using iohexol in mouse models show that a 6 h cumulative urine sample may be sufficient to evaluate small intestinal permeability. Increased urinary excretion of iohexol is correlated with increased severity of duodenitis, suggesting potential as a marker for intestinal damage assessment. Reducing cage periods from 24 to 6 hours can improve animal welfare during these tests.
Article
Biochemistry & Molecular Biology
Csaba Revesz, Anita A. Wasik, Maria Godo, Pal Tod, Sanna Lehtonen, Gabor Szenasi, Peter Hamar
Summary: The study reveals that perfusion with ice-cold saline can cause more severe injury than ischemia-reperfusion at body temperature. Saline independently causes serious injury, while preservation solutions are more beneficial especially at 4 degrees Celsius. Therefore, using preservation solutions in kidney transplantation can help improve ischemic recovery.
Review
Chemistry, Medicinal
Sanna Lehtonen
Article
Endocrinology & Metabolism
Sonja Lindfors, Zydrune Polianskyte-Prause, Rim Bouslama, Eero Lehtonen, Miia Mannerla, Harry Nisen, Jukka Tienari, Hanne Salmenkari, Richard Forsgard, Tuomas Mirtti, Markku Lehto, Per-Henrik Groop, Sanna Lehtonen
Summary: AdipoRon can alleviate renal inflammation in HFD-fed mice and improve glomerular inflammation and injury by reducing the expression of proinflammatory cytokines and inhibiting inflammatory signaling pathways, thereby decreasing apoptosis, migration, and inflammatory response.
Review
Physiology
Vincent Dumont, Sanna Lehtonen
Summary: PACSINs are a family of proteins involved in the regulation of cell cytoskeleton, intracellular trafficking and signaling. They play important roles in various physiological and tissue repair processes, and are associated with certain diseases.
Article
Biochemistry & Molecular Biology
Zydrune Polianskyte-Prause, Tuomas A. Tolvanen, Sonja Lindfors, Kanta Kon, Laura C. Hautala, Hong Wang, Tsutomu Wada, Hiroshi Tsuneki, Toshiyasu Sasaoka, Sanna Lehtonen
Summary: Ebselen is a potential treatment for diabetes-related disorders. It inhibits SHIP2 to ameliorate insulin resistance and act as an antioxidant. Ebselen directly binds to and inhibits the activity of SHIP2, improving insulin sensitivity, protecting liver tissue, and improving glucose tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Virology
Luz E. Cabrera, Constanze Schmotz, Moin A. Saleem, Sanna Lehtonen, Olli Vapalahti, Antti Vaheri, Satu Makela, Jukka Mustonen, Tomas Strandin
Summary: This study found that HPSE levels in urine were significantly increased during the acute phase of HFRS and strongly associated with kidney injury and disease severity. Furthermore, increased expression of HPSE was detected in orthohantavirus-infected podocytes. These findings suggest that HPSE may play a role in disrupting the endothelial glycocalyx in the kidneys of orthohantavirus-infected patients, leading to proteinuria.
Article
Biochemistry & Molecular Biology
Lan Zhao, Shuyuan Wang, Marit Ilves, Sanna Lehtonen, Leena Saikko, Hani El-Nezami, Harri Alenius, Piia Karisola
Summary: Consumers and manufacturers are exposed to nanosized zinc oxide (nZnO) and silver particles (nAg) via airways, but their biological effects are still not fully elucidated. This study used mice to examine the immune effects of nZnO and nAg exposure in the lungs. The results showed that the immune responses and gene expression profiles varied over time, and nZnO caused acute inflammation while nAg had long-lasting effects on both innate and adaptive immune pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Rim Bouslama, Vincent Dumont, Sonja Lindfors, Lassi Paavolainen, Jukka Tienari, Harry Nisen, Tuomas Mirtti, Moin A. Saleem, Daniel Gordin, Per-Henrik Groop, Shiro Suetsugu, Sanna Lehtonen
Summary: Changes in the podocytes, the glomerular epithelial cells, can lead to kidney dysfunction. The phosphorylation of PACSIN2 at serine 313 (S313) is increased in the glomeruli of rats with diabetic kidney disease, and this phosphorylation is associated with kidney dysfunction and increased free fatty acids. PACSIN2 phosphorylation is a dynamic process that regulates cell morphology and cytoskeletal arrangement in cooperation with N-WASP. The phosphorylation of PACSIN2 at S313 is important for regulating cytoskeletal reorganization.