Article
Urology & Nephrology
Marlies Sachs, Sebastian Wetzel, Julia Reichelt, Wiebke Sachs, Lisa Schebsdat, Stephanie Zielinski, Lisa Seipold, Lukas Heintz, Stephan A. Muller, Oliver Kretz, Maja Lindenmeyer, Thorsten Wiech, Tobias B. Huber, Renate Lullmann-Rauch, Stefan F. Lichtenthaler, Paul Saftig, Catherine Meyer-Schwesinger
Summary: ADAM10 mediates podocyte injury by cleaving injury-related cadherins such as N- and P-Cadherin. Induction of podocyte ADAM10 expression is observed in antibody-mediated injury, while ADAM10 deficiency can attenuate the injury process.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Catherine Meyer-Schwesinger, Lisa Seipold, Paul Saftig
Summary: In addition to its role in blood and bone physiology, the kidney is primarily responsible for filtering substances and regulating the electrolyte composition and acid-base balance of body fluids. A Disintegrin and Metalloproteases (ADAMs) play a crucial role in regulating cell adhesion, matrix adhesion, and signaling pathways in kidney diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Fred Armbrust, Kira Bickenbach, Tomas Koudelka, Andreas Tholey, Claus Pietrzik, Christoph Becker-Pauly
Summary: Meprin beta is a zinc-dependent metalloprotease with a unique cleavage specificity for acidic amino acids at the cleavage site. Phosphorylation of meprin beta affects its cell surface activity and substrate cleavage extent, leading to internalization and degradation. The involvement of PKC isoforms in meprin beta phosphorylation was also demonstrated in this study.
Article
Medicine, Research & Experimental
Huw J. Morgan, Elise Rees, Simone Lanfredini, Kate A. Powell, Jasmine Gore, Alex Gibbs, Charlotte Lovatt, Gemma E. Davies, Carlotta Olivero, Boris Y. Shorning, Giusy Tornillo, Alex Tonks, Richard Darley, Eddie C. Y. Wang, Girish K. Patel
Summary: This study found that CD200 protein in basal cell carcinoma may lead to the near absence of NK cells in the tumor microenvironment through ectodomain shedding. CD200 suppresses MAPK signaling in NK cells, blocking indirect and direct cell killing. Blocking CD200 inhibition can restore NK cell killing ability.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Kazuya Tsumagari, Chih-Hsiang Chang, Yasushi Ishihama
Summary: Ectodomain shedding is a proteolytic process that regulates membrane protein levels and functions. Dysregulated shedding is associated with severe diseases such as cancer and Alzheimer's disease. By generating large-scale, cell-type-specific maps of shedding cleavage sites, we identified a significant number of cell-type-specific cleavage sites.
Review
Neurosciences
Dolores Martin-de-Saavedra, Marc Dos Santos, Peter Penzes
Summary: This article provides an overview of the importance of ectodomain shedding in the nervous system, discussing the role of synaptic ectodomain shedding in autocrine and paracrine signaling. The article also highlights the use of cerebrospinal fluid analysis for identifying potential biomarkers for neuropsychiatric disorders.
TRENDS IN NEUROSCIENCES
(2022)
Article
Multidisciplinary Sciences
Erik Klapproth, Anke Witt, Pauline Klose, Johanna Wiedemann, Nikitha Vavilthota, Stephan R. Kuenzel, Susanne Kaemmerer, Mario Guenscht, David Sprott, Mathias Lesche, Fabian Rost, Andreas Dahl, Erik Rauch, Lars Kattner, Silvio Weber, Peter Mirtschink, Irakli Kopaliani, Kaomei Guan, Kristina Lorenz, Paul Saftig, Michael Wagner, Ali El-Armouche
Summary: After myocardial infarction, targeting the ADAM10/CX3CL1 axis can help regulate neutrophil recruitment, improve heart function, and reduce scar size by reducing inflammation and neutrophil infiltration.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Shuuichi Mori, Shigeaki Suzuki, Tetsuro Konishi, Naoki Kawaguchi, Masahiko Kishi, Satoshi Kuwabara, Kei Ishizuchi, Heying Zhou, Futoshi Shibasaki, Hiroki Tsumoto, Takuya Omura, Yuri Miura, Seijiro Mori, Mana Higashihara, Shigeo Murayama, Kazuhiro Shigemoto
Summary: Autoantibodies to muscle-specific tyrosine kinase (MuSK) proteins at the neuromuscular junction (NMJ) cause refractory generalized myasthenia gravis (MG) with dyspnea more frequently than other MG subtypes. The secretion of MuSK proteins from muscles into the bloodstream is induced by ectodomain shedding triggered by neuro-muscular junction failure. MuSK may be used as a biomarker for other neuromuscular diseases in preclinical studies, clinical diagnostics, therapeutics, and drug discovery.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Immunology
Leonardo Loch, Thiago Souza Onofre, Joao Paulo Ferreira Rodrigues, Nobuko Yoshida
Summary: Metacyclic trypomastigote forms of Trypanosoma cruzi release stage-specific surface molecules gp82 and gp90 to regulate host cell invasion. The release of vesicles by different strains leads to differences in protein profile and the distribution of gp82 and gp90. Treatments like cholesterol-depleting drugs and phospholipase C inhibitors affect the release of these molecules, impacting host cell invasion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fang-Ling Lin, Jui-Ting Yen, Pin-Wen Fang, Shun-Qiang Xu, Jing-Cyun Lin, Kui-Thong Tan
Summary: This paper describes a new strategy using an affinity-based protein-labeling fluorescent probe to study ectodomain shedding. The study identifies the ADAM17 metalloprotease as responsible for the shedding of carbonic anhydrases and shows that this protein-labeling approach provides a more detailed insight into the mechanism of ectodomain shedding and protein lifetime on the cell surface, compared to current immunological methods.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Johanna Tueshaus, Stephan A. Mueller, Joshua Shrouder, Martina Arends, Mikael Simons, Nikolaus Plesnila, Carl P. Blobel, Stefan F. Lichtenthaler
Summary: iRhom1, crucial for controlling membrane protein shedding in the mouse brain, is widely expressed throughout various brain regions, with a peak in early postnatal development. Proteomic analysis identified several membrane proteins that rely on iRhom1 for shedding, highlighting its importance in this process.
Article
Multidisciplinary Sciences
Emma Petley, Hui-Fern Koay, Melissa A. Henderson, Kevin Sek, Kirsten L. Todd, Simon P. Keam, Junyun Lai, Imran G. House, Jasmine Li, Magnus Zethoven, Amanda X. Y. Chen, Amanda J. Oliver, Jessica Michie, Andrew J. Freeman, Lauren Giuffrida, Jack D. Chan, Angela Pizzolla, Jeffrey Y. W. Mak, Timothy R. McCulloch, Fernando Souza-Fonseca-Guimaraes, Conor J. Kearney, Rosemary Millen, Robert G. Ramsay, Nicholas D. Huntington, James McCluskey, Jane Oliaro, David P. Fairlie, Paul J. Neeson, Dale Godfrey, Paul A. Beavis, Phillip K. Darcy
Summary: The study reveals that activated MAIT cells can induce an IFN-gamma transcriptome in NK cells and enhance NK-dependent anti-cancer immunity in mice, suggesting a new potential avenue for cancer therapy.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Manae Tatsumi, Takayuki Kishi, Satoru Ishida, Hiroki Kawana, Akiharu Uwamizu, Yuki Ono, Kouki Kawakami, Junken Aoki, Asuka Inoue
Summary: A method previously used for detecting GPCR activation has been shown to also detect TRP channel activation. The method is simple, low-cost, and only requires an absorbance microplate reader. Using substrates of ADAM10, such as EGF and BTC, improves the specificity of the assay.
Article
Medicine, Research & Experimental
Sebastian P. Gnosa, Laia Puig Blasco, Krzysztof B. Piotrowski, Marie L. Freiberg, Simonas Savickas, Daniel H. Madsen, Ulrich Auf Dem Keller, Pauliina Kronqvist, Marie Kveiborg
Summary: Macrophages in the tumor microenvironment play a significant role in tumor progression, and ADAM17 is identified as a key mediator in controlling macrophage education. Through a paracrine EGFR ligand-chemokine feedback loop, ADAM17 facilitates cancer cells in hijacking macrophages to promote tumor progression.
Article
Hematology
Annalena Fink, Anne-Katrin Rohlfing, Valerie Dicenta, David Schaale, Marcel Kremser, Zoi Laspa, Manuel Sigle, Xiaoqing Fu, Andreas Pelzer, Melina Fischer, Patrick Muenzer, Tatsiana Castor, Karin Anne Lydia Mueller, Oliver Borst, Michael Laemmerhofer, Meinrad Paul Gawaz
Summary: In this study, we found that hemin strongly enhances platelet activation, aggregation, and aggregate formation on immobilized collagen. The hemin-induced decrease in surface expression of GPVI is independent of platelet aggregation. We also discovered that the proprotein convertase furin controls hemin-induced GPVI degradation and platelet function.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Hisayo Nishida-Fukuda, Ryoichi Araki, Masachika Shudou, Hidenori Okazaki, Yasuko Tomono, Hironao Nakayama, Shinji Fukuda, Tomohisa Sakaue, Yuji Shirakata, Koji Sayama, Koji Hashimoto, Michael Detmar, Shigeki Higashiyama, Satoshi Hirakawa
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Shinji Fukuda, Hisayo Nishida-Fukuda, Daisuke Nanba, Koh-ichi Nakashiro, Hironao Nakayama, Hiroyuki Kubota, Shigeki Higashiyama
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Shinji Fukuda, Hisayo Nishida-Fukuda, Hironao Nakayama, Hirofumi Inoue, Shigeki Higashiyama
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2012)
Article
Biochemistry & Molecular Biology
Hironao Nakayama, Shinji Fukuda, Natsuki Matsushita, Hisayo Nishida-Fukuda, Hirofumi Inoue, Yuji Shirakata, Koji Hashimoto, Shigeki Higashiyama
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Correction
Multidisciplinary Sciences
Syed Mukhtar Ahmed, Hisayo Nishida-Fukuda, Yuchong Li, W. Hayes McDonald, Claudiu C. Gradinaru, Ian G. Macara
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Syed Mukhtar Ahmed, Hisayo Nishida-Fukuda, Yuchong Li, W. Hayes McDonald, Claudiu C. Gradinaru, Ian G. Macara
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Hisayo Nishida-Fukuda
Article
Biology
Satoshi Yamanaka, Yuki Shoya, Saya Matsuoka, Hisayo Nishida-Fukuda, Norio Shibata, Tatsuya Sawasaki
COMMUNICATIONS BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Hisayo Nishida-Fukuda, Keizo Tokuhiro, Yukio Ando, Hiroaki Matsushita, Morimasa Wada, Hiromitsu Tanaka
Summary: HASPIN, a kinase regulating mitosis, is associated with tumor malignancy and poor survival in various cancers. CHR-6494, a potent HASPIN inhibitor, showed antiproliferative effects in breast cancer cell lines and mammary epithelial cells, suggesting potential as an anticancer agent in breast cancer patients.
Article
Biochemistry & Molecular Biology
Hironao Nakayama, Hidetaka Ohnuki, Masako Nakahara, Hisayo Nishida-Fukuda, Tomohisa Sakaue, Shinji Fukuda, Shigeki Higashiyama, Yuki Doi, Masahiro Mitsuyoshi, Takashi Okimoto, Giovanna Tosato, Chiaki Kusumoto
Summary: Netrin-1 expression is significantly reduced in colorectal cancer tissues compared to normal tissues, and DNA methylation plays a role in the repression of NTN1 transcription in CRC. NTN1 DNA hypermethylation is associated with advanced CRC disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Hiromitsu Tanaka, Hisayo Nishida-Fukuda, Morimasa Wada, Keizo Tokuhiro, Hiroaki Matsushita, Yukio Ando
Summary: It has been discovered that the HASPIN inhibitor CHR-6494 can suppress the growth of pancreatic cancer cells, making it a potential treatment for pancreatic cancer.
Meeting Abstract
Cell Biology
H. Nishida-Fukuda, I. G. Macara, S. M. Ahmed
MOLECULAR BIOLOGY OF THE CELL
(2017)
Meeting Abstract
Cell Biology
H. Nishida-Fukuda, I. G. Macara, S. M. Ahmed
MOLECULAR BIOLOGY OF THE CELL
(2017)
Meeting Abstract
Dermatology
S. Hirakawa, H. Nishida-Fukuda, M. Detmar, S. Higashiyama, Y. Tokura
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2012)